Restriction map for
pF1KE0120
(Circular) (Six-base) MAPSORT of: pF1KE0120.seq Check: 3891 from: 1 to: 3974
pF1KE0120 3974 bp
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AccI GT'mk_AC
Cuts at: 683 884 1012 683
Size: 201 128 3645
Fragments arranged by size:
3645 201 128
Acc65I G'GTAC_C
Cuts at: 990 990
Size: 3974
AclI AA'CG_TT
Cuts at: 3814 3814
Size: 3974
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 394 1799 2231 2978 3246 394
Size: 1405 432 747 268 1122
Fragments arranged by size:
1405 1122 747 432 268
AflII C'TTAA_G
Cuts at: 919 919
Size: 3974
AflIII A'CryG_T
Cuts at: 1326 2430 3407 1326
Size: 1104 977 1893
Fragments arranged by size:
1893 1104 977
AgeI A'CCGG_T
Cuts at: 2362 2362
Size: 3974
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 362 362
Size: 3974
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 145 177 145
Size: 32 3942
AlwNI CAG_nnn'CTG
Cuts at: 399 822 950 1279 2846 399
Size: 423 128 329 1567 1527
Fragments arranged by size:
1567 1527 423 329 128
ApaI G_GGCC'C
Cuts at: 272 272
Size: 3974
ApaLI G'TGCA_C
Cuts at: 187 838 2744 187
Size: 651 1906 1417
Fragments arranged by size:
1906 1417 651
ApoI r'AATT_y
Cuts at: 978 3327 3583 978
Size: 2349 256 1369
Fragments arranged by size:
2349 1369 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3974
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3974
AvaI C'yCGr_G
Cuts at: 613 994 613
Size: 381 3593
AvrII C'CTAG_G
Cuts at: 294 294
Size: 3974
BamHI G'GATC_C
Cuts at: 999 999
Size: 3974
BanI G'GyrC_C
Cuts at: 990 1404 1652 1687 990
Size: 414 248 35 3277
Fragments arranged by size:
3277 414 248 35
BanII G_rGCy'C
Cuts at: 256 272 576 814 988 2018 256
Size: 16 304 238 174 1030 2212
Fragments arranged by size:
2212 1030 304 238 174 16
BbeI G_GCGC'C
Cuts at: 1408 1656 1408
Size: 248 3726
BbvCI CC'TCA_GC
Cuts at: 352 527 352
Size: 175 3799
BciVI GTATCCnnnnn_n'
Cuts at: 1872 2633 3974 1872
Size: 761 1341 1872
Fragments arranged by size:
1872 1341 761
BclI T'GATC_A
Cuts at: 1494 1494
Size: 3974
BglII A'GATC_T
Cuts at: 929 1489 3526 929
Size: 560 2037 1377
Fragments arranged by size:
2037 1377 560
BlpI GC'TnA_GC
Cuts at: 1086 1086
Size: 3974
Bme1580I G_kGCm'C
Cuts at: 191 272 842 1599 1692 2748 191
Size: 81 570 757 93 1056 1417
Fragments arranged by size:
1417 1056 757 570 93 81
BmrI ACTGGGnnnn_n'
Cuts at: 1186 1267 1602 3746 1186
Size: 81 335 2144 1414
Fragments arranged by size:
2144 1414 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 828 2341 828
Size: 1513 2461
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 172 844 927 972 172
Size: 672 83 45 3174
Fragments arranged by size:
3174 672 83 45
Bpu10I CC'TnA_GC
Cuts at: 352 527 352
Size: 175 3799
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 205 520 1150 2029 2521 2819 205
Size: 315 630 879 492 298 1360
Fragments arranged by size:
1360 879 630 492 315 298
BsaI GGTCTCn'nnnn_
Cuts at: 3678 3678
Size: 3974
BsaAI yAC'GTr
Cuts at: 837 1327 837
Size: 490 3484
BsaBI GATnn'nnATC
Cuts at: 6 1488 3525 6
Size: 1482 2037 455
Fragments arranged by size:
2037 1482 455
BsaHI Gr'CG_yC
Cuts at: 875 1405 1653 2355 3851 875
Size: 530 248 702 1496 998
Fragments arranged by size:
1496 998 702 530 248
BsaWI w'CCGG_w
Cuts at: 1163 1684 2362 2636 2783 3194 1163
Size: 521 678 274 147 411 1943
Fragments arranged by size:
1943 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 145 175 916 946 145
Size: 30 741 30 3173
Fragments arranged by size:
3173 741 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 169 172 169
Size: 3 3971
BseYI C'CCAG_C
Cuts at: 762 766 1399 2157 2734 3230 762
Size: 4 633 758 577 496 1506
Fragments arranged by size:
1506 758 633 577 496 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 160 385 160
Size: 225 3749
BsiEI CG_ry'CG
Cuts at: 104 1516 1562 2770 104
Size: 1412 46 1208 1308
Fragments arranged by size:
1412 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 191 842 988 1766 1956 2748 191
Size: 651 146 778 190 792 1417
Fragments arranged by size:
1417 792 778 651 190 146
BsmBI CGTCTCn'nnnn_
Cuts at: 900 900
Size: 3974
Bsp1286I G_dGCh'C
Cuts at: 191 256 272 576 814 842 988 1599 1692
Size: 65 16 304 238 28 146 611 93
Cuts at: 1692 1766 1956 2018 2748 191
Size: 74 190 62 730 1417
Fragments arranged by size:
1417 730 611 304 238 190 146 93
74 65 62 28 16
BspEI T'CCGG_A
Cuts at: 3194 3194
Size: 3974
BspHI T'CATG_A
Cuts at: 3150 3150
Size: 3974
BspMI ACCTGCnnnn'nnnn_
Cuts at: 491 677 1024 1238 1540 1921 491
Size: 186 347 214 302 381 2544
Fragments arranged by size:
2544 381 347 302 214 186
BsrBI CCG'CTC
Cuts at: 50 141 2266 2320 3804 3969 50
Size: 91 2125 54 1484 165 55
Fragments arranged by size:
2125 1484 165 91 55 54
BsrDI GCAATG_nn'
Cuts at: 1886 1886
Size: 3974
BsrFI r'CCGG_y
Cuts at: 581 1972 2153 2362 581
Size: 1391 181 209 2193
Fragments arranged by size:
2193 1391 209 181
BssSI C'ACGA_G
Cuts at: 2245 2603 2245
Size: 358 3616
BstAPI GCAn_nnn'nTGC
Cuts at: 732 1351 732
Size: 619 3355
BstBI TT'CG_AA
Cuts at: 2335 2335
Size: 3974
BstXI CCAn_nnnn'nTGG
Cuts at: 120 120
Size: 3974
BstYI r'GATC_y
Cuts at: 1 443 929 999 1466 1489 1824 2070 3082
Size: 442 486 70 467 23 335 246 1012
Cuts at: 3082 3168 3180 3526 1
Size: 86 12 346 449
Fragments arranged by size:
1012 486 467 449 442 346 335 246
86 70 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3974
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1924 2105 2268 1924
Size: 181 163 3630
Fragments arranged by size:
3630 181 163
BtsI GCAGTG_nn'
Cuts at: 433 557 1217 433
Size: 124 660 3190
Fragments arranged by size:
3190 660 124
DraI TTT'AAA
Cuts at: 973 973
Size: 3974
DraIII CAC_nnn'GTG
Cuts at: 195 846 195
Size: 651 3323
DrdI GACnn_nn'nnGTC
Cuts at: 673 880 1680 2538 673
Size: 207 800 858 2109
Fragments arranged by size:
2109 858 800 207
EaeI y'GGCC_r
Cuts at: 110 1559 1733 2124 2151 3496 110
Size: 1449 174 391 27 1345 588
Fragments arranged by size:
1449 1345 588 391 174 27
EagI C'GGCC_G
Cuts at: 1559 1559
Size: 3974
EarI CTCTTCn'nnn_
Cuts at: 1997 2207 1997
Size: 210 3764
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 151 376 748 2182 2492 2638 3784 151
Size: 225 372 1434 310 146 1146 341
Fragments arranged by size:
1434 1146 372 341 310 225 146
EcoICRI GAG'CTC
Cuts at: 986 986
Size: 3974
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 172 394 844 927 972 1799 2231 2978 3246
Size: 222 450 83 45 827 432 747 268
Cuts at: 3246 172
Size: 900
Fragments arranged by size:
900 827 747 450 432 268 222 83
45
EcoNI CCTnn'n_nnAGG
Cuts at: 223 355 223
Size: 132 3842
EcoO109I rG'GnC_Cy
Cuts at: 268 531 1113 268
Size: 263 582 3129
Fragments arranged by size:
3129 582 263
EcoRI G'AATT_C
Cuts at: 978 978
Size: 3974
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1993 1993
Size: 3974
FspI TGC'GCA
Cuts at: 1755 1755
Size: 3974
HaeII r_GCGC'y
Cuts at: 527 822 1408 1656 2678 3657 527
Size: 295 586 248 1022 979 844
Fragments arranged by size:
1022 979 844 586 295 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 916 948 1816 1846 1848 1878 916
Size: 32 868 30 2 30 3012
Fragments arranged by size:
3012 868 32 30 30 2
HincII GTy'rAC
Cuts at: 678 1013 678
Size: 335 3639
KasI G'GCGC_C
Cuts at: 1404 1652 1404
Size: 248 3726
KpnI G_GTAC'C
Cuts at: 994 994
Size: 3974
MluI A'CGCG_T
Cuts at: 3407 3407
Size: 3974
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 689 2645 2829 689
Size: 1956 184 1834
Fragments arranged by size:
1956 1834 184
MscI TGG'CCA
Cuts at: 112 1735 112
Size: 1623 2351
MslI CAynn'nnrTG
Cuts at: 118 640 1963 2090 118
Size: 522 1323 127 2002
Fragments arranged by size:
2002 1323 522 127
MspA1I CmG'CkG
Cuts at: 167 207 302 396 564 629 723 766 1085
Size: 40 95 94 168 65 94 43 319
Cuts at: 1085 1399 1759 2772 3017 167
Size: 314 360 1013 245 1124
Fragments arranged by size:
1124 1013 360 319 314 245 168 95
94 94 65 43 40
NaeI GCC'GGC
Cuts at: 2155 2155
Size: 3974
NarI GG'CG_CC
Cuts at: 1405 1653 1405
Size: 248 3726
NcoI C'CATG_G
Cuts at: 107 107
Size: 3974
NgoMIV G'CCGG_C
Cuts at: 2153 2153
Size: 3974
NspI r_CATG'y
Cuts at: 730 1027 1527 2434 730
Size: 297 500 907 2270
Fragments arranged by size:
2270 907 500 297
PasI CC'CwG_GG
Cuts at: 790 790
Size: 3974
PciI A'CATG_T
Cuts at: 2430 2430
Size: 3974
PflMI CCAn_nnn'nTGG
Cuts at: 950 950
Size: 3974
PfoI T'CCnGG_A
Cuts at: 413 446 413
Size: 33 3941
PmeI GTTT'AAAC
Cuts at: 973 973
Size: 3974
PmlI CAC'GTG
Cuts at: 837 837
Size: 3974
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 145 177 3113 3145 145
Size: 32 2936 32 974
Fragments arranged by size:
2936 974 32 32
PpuMI rG'GwC_Cy
Cuts at: 531 531
Size: 3974
PspOMI G'GGCC_C
Cuts at: 268 268
Size: 3974
PstI C_TGCA'G
Cuts at: 212 341 440 563 737 905 1021 1706 212
Size: 129 99 123 174 168 116 685 2480
Fragments arranged by size:
2480 685 174 168 129 123 116 99
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3974
PvuII CAG'CTG
Cuts at: 207 302 396 564 723 766 1399 1759 207
Size: 95 94 168 159 43 633 360 2422
Fragments arranged by size:
2422 633 360 168 159 95 94 43
RsrII CG'GwC_CG
Cuts at: 2169 2169
Size: 3974
SacI G_AGCT'C
Cuts at: 988 988
Size: 3974
SalI G'TCGA_C
Cuts at: 1011 1011
Size: 3974
SapI GCTCTTCn'nnn_
Cuts at: 1997 2207 1997
Size: 210 3764
SbfI CC_TGCA'GG
Cuts at: 1021 1021
Size: 3974
ScaI AGT'ACT
Cuts at: 3491 3491
Size: 3974
SexAI A'CCwGG_T
Cuts at: 314 314
Size: 3974
SfcI C'TryA_G
Cuts at: 33 208 337 436 559 733 901 957 1017
Size: 175 129 99 123 174 168 56 60
Cuts at: 1017 1702 2695 2886 33
Size: 685 993 191 1121
Fragments arranged by size:
1121 993 685 191 175 174 168 129
123 99 60 56
SfoI GGC'GCC
Cuts at: 1406 1654 1406
Size: 248 3726
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3974
SmaI CCC'GGG
Cuts at: 996 996
Size: 3974
SmlI C'TyrA_G
Cuts at: 220 535 919 1129 2044 2536 2798 220
Size: 315 384 210 915 492 262 1396
Fragments arranged by size:
1396 915 492 384 315 262 210
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3974
SphI G_CATG'C
Cuts at: 730 1027 1527 730
Size: 297 500 3177
Fragments arranged by size:
3177 500 297
StuI AGG'CCT
Cuts at: 229 229
Size: 3974
StyI C'CwwG_G
Cuts at: 107 294 1108 1462 107
Size: 187 814 354 2619
Fragments arranged by size:
2619 814 354 187
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1557 1690 2149 2358 1557
Size: 133 459 209 3173
Fragments arranged by size:
3173 459 209 133
TatI w'GTAC_w
Cuts at: 3489 3489
Size: 3974
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 412 444 3491 3523 412
Size: 32 3047 32 863
Fragments arranged by size:
3047 863 32 32
Tth111I GACn'n_nGTC
Cuts at: 1771 1771
Size: 3974
XbaI T'CTAG_A
Cuts at: 1005 1005
Size: 3974
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1469 1469
Size: 3974
XmaI C'CCGG_G
Cuts at: 994 994
Size: 3974
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlfI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
AvrII BamHI BanI BanII BbeI BbvCI BciVI BclI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AscI BaeI BbsI
BcgI BfrBI BglI BmgBI BmtI BsiWI BsmI BsrGI
BssHII BstEII BstZ17I Bsu36I ClaI CspCI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PshAI PsiI PspXI PsrI SacII SanDI
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI XmnI
ZraI