Restriction map for
pF1KE0121
(Circular) (Six-base) MAPSORT of: pF1KE0121.seq Check: 2760 from: 1 to: 3494
pF1KE0121 3494 bp
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AccI GT'mk_AC
Cuts at: 532 532
Size: 3494
Acc65I G'GTAC_C
Cuts at: 510 510
Size: 3494
AclI AA'CG_TT
Cuts at: 3334 3334
Size: 3494
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1319 1751 2498 2766 1319
Size: 432 747 268 2047
Fragments arranged by size:
2047 747 432 268
AflIII A'CryG_T
Cuts at: 846 1950 2927 846
Size: 1104 977 1413
Fragments arranged by size:
1413 1104 977
AgeI A'CCGG_T
Cuts at: 1882 1882
Size: 3494
AlwNI CAG_nnn'CTG
Cuts at: 799 2366 799
Size: 1567 1927
ApaLI G'TGCA_C
Cuts at: 2264 2264
Size: 3494
ApoI r'AATT_y
Cuts at: 498 2847 3103 498
Size: 2349 256 889
Fragments arranged by size:
2349 889 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3494
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3494
AvaI C'yCGr_G
Cuts at: 125 181 514 125
Size: 56 333 3105
Fragments arranged by size:
3105 333 56
BamHI G'GATC_C
Cuts at: 519 519
Size: 3494
BanI G'GyrC_C
Cuts at: 121 510 924 1172 1207 121
Size: 389 414 248 35 2408
Fragments arranged by size:
2408 414 389 248 35
BanII G_rGCy'C
Cuts at: 243 508 1538 243
Size: 265 1030 2199
Fragments arranged by size:
2199 1030 265
BbeI G_GCGC'C
Cuts at: 928 1176 928
Size: 248 3246
BciVI GTATCCnnnnn_n'
Cuts at: 1392 2153 3494 1392
Size: 761 1341 1392
Fragments arranged by size:
1392 1341 761
BclI T'GATC_A
Cuts at: 1014 1014
Size: 3494
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 3494
BglII A'GATC_T
Cuts at: 1009 3046 1009
Size: 2037 1457
BlpI GC'TnA_GC
Cuts at: 606 606
Size: 3494
Bme1580I G_kGCm'C
Cuts at: 126 1119 1212 2268 126
Size: 993 93 1056 1352
Fragments arranged by size:
1352 1056 993 93
BmrI ACTGGGnnnn_n'
Cuts at: 706 787 1122 3266 706
Size: 81 335 2144 934
Fragments arranged by size:
2144 934 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1861 1861
Size: 3494
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 670 1549 2041 2339 670
Size: 879 492 298 1825
Fragments arranged by size:
1825 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3198 3198
Size: 3494
BsaAI yAC'GTr
Cuts at: 847 847
Size: 3494
BsaBI GATnn'nnATC
Cuts at: 6 1008 3045 6
Size: 1002 2037 455
Fragments arranged by size:
2037 1002 455
BsaHI Gr'CG_yC
Cuts at: 925 1173 1875 3371 925
Size: 248 702 1496 1048
Fragments arranged by size:
1496 1048 702 248
BsaWI w'CCGG_w
Cuts at: 683 1204 1882 2156 2303 2714 683
Size: 521 678 274 147 411 1463
Fragments arranged by size:
1463 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 145 145
Size: 3494
BseYI C'CCAG_C
Cuts at: 919 1677 2254 2750 919
Size: 758 577 496 1663
Fragments arranged by size:
1663 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 116 1036 1082 2290 104
Size: 12 920 46 1208 1308
Fragments arranged by size:
1308 1208 920 46 12
BsiHKAI G_wGCw'C
Cuts at: 508 1286 1476 2268 508
Size: 778 190 792 1734
Fragments arranged by size:
1734 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 126 243 508 1119 1212 1286 1476 1538 2268
Size: 117 265 611 93 74 190 62 730
Cuts at: 2268 126
Size: 1352
Fragments arranged by size:
1352 730 611 265 190 117 93 74
62
BspEI T'CCGG_A
Cuts at: 2714 2714
Size: 3494
BspHI T'CATG_A
Cuts at: 2670 2670
Size: 3494
BspMI ACCTGCnnnn'nnnn_
Cuts at: 205 303 544 758 1060 1441 205
Size: 98 241 214 302 381 2258
Fragments arranged by size:
2258 381 302 241 214 98
BsrBI CCG'CTC
Cuts at: 50 1786 1840 3324 3489 50
Size: 1736 54 1484 165 55
Fragments arranged by size:
1736 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1406 1406
Size: 3494
BsrFI r'CCGG_y
Cuts at: 118 149 1492 1673 1882 118
Size: 31 1343 181 209 1730
Fragments arranged by size:
1730 1343 209 181 31
BsrGI T'GTAC_A
Cuts at: 200 200
Size: 3494
BssSI C'ACGA_G
Cuts at: 1765 2123 1765
Size: 358 3136
BstAPI GCAn_nnn'nTGC
Cuts at: 871 871
Size: 3494
BstBI TT'CG_AA
Cuts at: 1855 1855
Size: 3494
BstXI CCAn_nnnn'nTGG
Cuts at: 386 386
Size: 3494
BstYI r'GATC_y
Cuts at: 1 519 986 1009 1344 1590 2602 2688 2700
Size: 518 467 23 335 246 1012 86 12
Cuts at: 2700 3046 1
Size: 346 449
Fragments arranged by size:
1012 518 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1444 1625 1788 1444
Size: 181 163 3150
Fragments arranged by size:
3150 181 163
BtsI GCAGTG_nn'
Cuts at: 737 737
Size: 3494
DraI TTT'AAA
Cuts at: 493 493
Size: 3494
DrdI GACnn_nn'nnGTC
Cuts at: 1200 2058 1200
Size: 858 2636
EaeI y'GGCC_r
Cuts at: 113 287 1079 1253 1644 1671 3016 113
Size: 174 792 174 391 27 1345 591
Fragments arranged by size:
1345 792 591 391 174 174 27
EagI C'GGCC_G
Cuts at: 113 1079 113
Size: 966 2528
EarI CTCTTCn'nnn_
Cuts at: 1517 1727 1517
Size: 210 3284
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1702 2012 2158 3304 1702
Size: 310 146 1146 1892
Fragments arranged by size:
1892 1146 310 146
EcoICRI GAG'CTC
Cuts at: 506 506
Size: 3494
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1319 1751 2498 2766 1319
Size: 432 747 268 2047
Fragments arranged by size:
2047 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 217 633 217
Size: 416 3078
EcoRI G'AATT_C
Cuts at: 498 498
Size: 3494
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1513 1513
Size: 3494
FspI TGC'GCA
Cuts at: 1275 1275
Size: 3494
HaeII r_GCGC'y
Cuts at: 928 1176 2198 3177 928
Size: 248 1022 979 1245
Fragments arranged by size:
1245 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1336 1366 1368 1398 1336
Size: 30 2 30 3432
Fragments arranged by size:
3432 30 30 2
HincII GTy'rAC
Cuts at: 533 533
Size: 3494
KasI G'GCGC_C
Cuts at: 924 1172 924
Size: 248 3246
KpnI G_GTAC'C
Cuts at: 514 514
Size: 3494
MluI A'CGCG_T
Cuts at: 2927 2927
Size: 3494
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2165 2349 2165
Size: 184 3310
MscI TGG'CCA
Cuts at: 289 1255 289
Size: 966 2528
MslI CAynn'nnrTG
Cuts at: 1483 1610 1483
Size: 127 3367
MspA1I CmG'CkG
Cuts at: 605 919 1279 2292 2537 605
Size: 314 360 1013 245 1562
Fragments arranged by size:
1562 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1675 1675
Size: 3494
NarI GG'CG_CC
Cuts at: 925 1173 925
Size: 248 3246
NgoMIV G'CCGG_C
Cuts at: 1673 1673
Size: 3494
NspI r_CATG'y
Cuts at: 547 1047 1954 547
Size: 500 907 2087
Fragments arranged by size:
2087 907 500
PciI A'CATG_T
Cuts at: 1950 1950
Size: 3494
PmeI GTTT'AAAC
Cuts at: 493 493
Size: 3494
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2633 2665 2633
Size: 32 3462
PpuMI rG'GwC_Cy
Cuts at: 217 217
Size: 3494
PstI C_TGCA'G
Cuts at: 300 541 1226 300
Size: 241 685 2568
Fragments arranged by size:
2568 685 241
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3494
PvuII CAG'CTG
Cuts at: 919 1279 919
Size: 360 3134
RsrII CG'GwC_CG
Cuts at: 1689 1689
Size: 3494
SacI G_AGCT'C
Cuts at: 508 508
Size: 3494
SalI G'TCGA_C
Cuts at: 531 531
Size: 3494
SapI GCTCTTCn'nnn_
Cuts at: 1517 1727 1517
Size: 210 3284
SbfI CC_TGCA'GG
Cuts at: 541 541
Size: 3494
ScaI AGT'ACT
Cuts at: 3011 3011
Size: 3494
SfcI C'TryA_G
Cuts at: 33 296 338 537 1222 2215 2406 33
Size: 263 42 199 685 993 191 1121
Fragments arranged by size:
1121 993 685 263 199 191 42
SfoI GGC'GCC
Cuts at: 926 1174 926
Size: 248 3246
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3494
SgrAI Cr'CCGG_yG
Cuts at: 118 118
Size: 3494
SmaI CCC'GGG
Cuts at: 516 516
Size: 3494
SmlI C'TyrA_G
Cuts at: 649 1564 2056 2318 649
Size: 915 492 262 1825
Fragments arranged by size:
1825 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3494
SphI G_CATG'C
Cuts at: 547 1047 547
Size: 500 2994
StuI AGG'CCT
Cuts at: 131 131
Size: 3494
StyI C'CwwG_G
Cuts at: 628 982 628
Size: 354 3140
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1077 1210 1669 1878 1077
Size: 133 459 209 2693
Fragments arranged by size:
2693 459 209 133
TatI w'GTAC_w
Cuts at: 200 3009 200
Size: 2809 685
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3011 3043 3011
Size: 32 3462
Tth111I GACn'n_nGTC
Cuts at: 1291 1291
Size: 3494
XbaI T'CTAG_A
Cuts at: 525 525
Size: 3494
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 251 989 251
Size: 738 2756
XmaI C'CCGG_G
Cuts at: 514 514
Size: 3494
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PciI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BmgBI BmtI BpmI Bpu10I BsaXI BsgI BsiWI BsmI
BsmBI BssHII BstEII BstZ17I Bsu36I BtgI ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmlI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SnaBI SrfI SspI SwaI
XhoI XmnI ZraI