Restriction map for
pF1KE0123
(Circular) (Six-base) MAPSORT of: pF1KE0123.seq Check: 176 from: 1 to: 3980
pF1KE0123 3980 bp
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AccI GT'mk_AC
Cuts at: 1018 1018
Size: 3980
Acc65I G'GTAC_C
Cuts at: 996 996
Size: 3980
AclI AA'CG_TT
Cuts at: 3820 3820
Size: 3980
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 988 1805 2237 2984 3252 988
Size: 817 432 747 268 1716
Fragments arranged by size:
1716 817 747 432 268
AfeI AGC'GCT
Cuts at: 180 180
Size: 3980
AflIII A'CryG_T
Cuts at: 1332 2436 3413 1332
Size: 1104 977 1899
Fragments arranged by size:
1899 1104 977
AgeI A'CCGG_T
Cuts at: 2368 2368
Size: 3980
AlwNI CAG_nnn'CTG
Cuts at: 891 1285 2852 891
Size: 394 1567 2019
Fragments arranged by size:
2019 1567 394
ApaI G_GGCC'C
Cuts at: 870 900 870
Size: 30 3950
ApaLI G'TGCA_C
Cuts at: 2750 2750
Size: 3980
ApoI r'AATT_y
Cuts at: 984 3333 3589 984
Size: 2349 256 1375
Fragments arranged by size:
2349 1375 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3980
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3980
AvaI C'yCGr_G
Cuts at: 218 547 671 1000 218
Size: 329 124 329 3198
Fragments arranged by size:
3198 329 329 124
BamHI G'GATC_C
Cuts at: 582 1005 582
Size: 423 3557
BanI G'GyrC_C
Cuts at: 913 996 1410 1658 1693 913
Size: 83 414 248 35 3200
Fragments arranged by size:
3200 414 248 83 35
BanII G_rGCy'C
Cuts at: 548 870 900 994 2024 548
Size: 322 30 94 1030 2504
Fragments arranged by size:
2504 1030 322 94 30
BbeI G_GCGC'C
Cuts at: 1414 1662 1414
Size: 248 3732
BciVI GTATCCnnnnn_n'
Cuts at: 396 1878 2639 3980 396
Size: 1482 761 1341 396
Fragments arranged by size:
1482 1341 761 396
BclI T'GATC_A
Cuts at: 1500 1500
Size: 3980
BglII A'GATC_T
Cuts at: 1495 3532 1495
Size: 2037 1943
BlpI GC'TnA_GC
Cuts at: 1092 1092
Size: 3980
Bme1580I G_kGCm'C
Cuts at: 870 900 1605 1698 2754 870
Size: 30 705 93 1056 2096
Fragments arranged by size:
2096 1056 705 93 30
BmrI ACTGGGnnnn_n'
Cuts at: 1192 1273 1608 3752 1192
Size: 81 335 2144 1420
Fragments arranged by size:
2144 1420 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2347 2347
Size: 3980
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 197 844 197
Size: 647 3333
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 733 1156 2035 2527 2825 733
Size: 423 879 492 298 1888
Fragments arranged by size:
1888 879 492 423 298
BsaI GGTCTCn'nnnn_
Cuts at: 3684 3684
Size: 3980
BsaAI yAC'GTr
Cuts at: 1333 1333
Size: 3980
BsaBI GATnn'nnATC
Cuts at: 6 1494 3531 6
Size: 1488 2037 455
Fragments arranged by size:
2037 1488 455
BsaHI Gr'CG_yC
Cuts at: 144 1411 1659 2361 3857 144
Size: 1267 248 702 1496 267
Fragments arranged by size:
1496 1267 702 267 248
BsaWI w'CCGG_w
Cuts at: 1169 1690 2368 2642 2789 3200 1169
Size: 521 678 274 147 411 1949
Fragments arranged by size:
1949 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 134 164 817 847 134
Size: 30 653 30 3267
Fragments arranged by size:
3267 653 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 129 556 841 844 129
Size: 427 285 3 3265
Fragments arranged by size:
3265 427 285 3
BseYI C'CCAG_C
Cuts at: 175 352 395 652 743 1405 2163 2740 3236
Size: 177 43 257 91 662 758 577 496
Cuts at: 3236 175
Size: 919
Fragments arranged by size:
919 758 662 577 496 257 177 91
43
BsiEI CG_ry'CG
Cuts at: 104 163 1522 1568 2776 104
Size: 59 1359 46 1208 1308
Fragments arranged by size:
1359 1308 1208 59 46
BsiHKAI G_wGCw'C
Cuts at: 683 946 994 1772 1962 2754 683
Size: 263 48 778 190 792 1909
Fragments arranged by size:
1909 792 778 263 190 48
BsmBI CGTCTCn'nnnn_
Cuts at: 154 215 154
Size: 61 3919
Bsp1286I G_dGCh'C
Cuts at: 548 683 870 900 946 994 1605 1698 1772
Size: 135 187 30 46 48 611 93 74
Cuts at: 1772 1962 2024 2754 548
Size: 190 62 730 1774
Fragments arranged by size:
1774 730 611 190 187 135 93 74
62 48 46 30
BspEI T'CCGG_A
Cuts at: 3200 3200
Size: 3980
BspHI T'CATG_A
Cuts at: 3156 3156
Size: 3980
BspMI ACCTGCnnnn'nnnn_
Cuts at: 791 1030 1244 1546 1927 791
Size: 239 214 302 381 2844
Fragments arranged by size:
2844 381 302 239 214
BsrBI CCG'CTC
Cuts at: 50 2272 2326 3810 3975 50
Size: 2222 54 1484 165 55
Fragments arranged by size:
2222 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 368 1892 368
Size: 1524 2456
BsrFI r'CCGG_y
Cuts at: 162 910 1978 2159 2368 162
Size: 748 1068 181 209 1774
Fragments arranged by size:
1774 1068 748 209 181
BssSI C'ACGA_G
Cuts at: 2251 2609 2251
Size: 358 3622
BstAPI GCAn_nnn'nTGC
Cuts at: 807 891 1357 807
Size: 84 466 3430
Fragments arranged by size:
3430 466 84
BstBI TT'CG_AA
Cuts at: 2341 2341
Size: 3980
BstXI CCAn_nnnn'nTGG
Cuts at: 375 645 375
Size: 270 3710
BstYI r'GATC_y
Cuts at: 1 582 839 1005 1472 1495 1830 2076 3088
Size: 581 257 166 467 23 335 246 1012
Cuts at: 3088 3174 3186 3532 1
Size: 86 12 346 449
Fragments arranged by size:
1012 581 467 449 346 335 257 246
166 86 23 12
BtgI C'CryG_G
Cuts at: 755 755
Size: 3980
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1930 2111 2274 1930
Size: 181 163 3636
Fragments arranged by size:
3636 181 163
BtsI GCAGTG_nn'
Cuts at: 380 1223 380
Size: 843 3137
DraI TTT'AAA
Cuts at: 979 979
Size: 3980
DraIII CAC_nnn'GTG
Cuts at: 280 440 280
Size: 160 3820
DrdI GACnn_nn'nnGTC
Cuts at: 1686 2544 1686
Size: 858 3122
EaeI y'GGCC_r
Cuts at: 160 365 1565 1739 2130 2157 3502 160
Size: 205 1200 174 391 27 1345 638
Fragments arranged by size:
1345 1200 638 391 205 174 27
EagI C'GGCC_G
Cuts at: 160 1565 160
Size: 1405 2575
EarI CTCTTCn'nnn_
Cuts at: 241 925 2003 2213 241
Size: 684 1078 210 2008
Fragments arranged by size:
2008 1078 684 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2188 2498 2644 3790 2188
Size: 310 146 1146 2378
Fragments arranged by size:
2378 1146 310 146
EcoICRI GAG'CTC
Cuts at: 992 992
Size: 3980
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 197 844 988 1805 2237 2984 3252 197
Size: 647 144 817 432 747 268 925
Fragments arranged by size:
925 817 747 647 432 268 144
EcoNI CCTnn'n_nnAGG
Cuts at: 826 970 826
Size: 144 3836
EcoO109I rG'GnC_Cy
Cuts at: 360 649 1119 360
Size: 289 470 3221
Fragments arranged by size:
3221 470 289
EcoRI G'AATT_C
Cuts at: 984 984
Size: 3980
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1999 1999
Size: 3980
FspI TGC'GCA
Cuts at: 1761 1761
Size: 3980
HaeII r_GCGC'y
Cuts at: 182 1414 1662 2684 3663 182
Size: 1232 248 1022 979 499
Fragments arranged by size:
1232 1022 979 499 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 134 166 240 272 641 673 1822 1852 1854
Size: 32 74 32 369 32 1149 30 2
Cuts at: 1854 1884 134
Size: 30 2230
Fragments arranged by size:
2230 1149 369 74 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1019 1019
Size: 3980
KasI G'GCGC_C
Cuts at: 1410 1658 1410
Size: 248 3732
KpnI G_GTAC'C
Cuts at: 1000 1000
Size: 3980
MluI A'CGCG_T
Cuts at: 3413 3413
Size: 3980
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2651 2835 2651
Size: 184 3796
MscI TGG'CCA
Cuts at: 367 1741 367
Size: 1374 2606
MslI CAynn'nnrTG
Cuts at: 373 875 1969 2096 373
Size: 502 1094 127 2257
Fragments arranged by size:
2257 1094 502 127
MspA1I CmG'CkG
Cuts at: 486 1091 1405 1765 2778 3023 486
Size: 605 314 360 1013 245 1443
Fragments arranged by size:
1443 1013 605 360 314 245
NaeI GCC'GGC
Cuts at: 164 912 2161 164
Size: 748 1249 1983
Fragments arranged by size:
1983 1249 748
NarI GG'CG_CC
Cuts at: 1411 1659 1411
Size: 248 3732
NcoI C'CATG_G
Cuts at: 755 755
Size: 3980
NgoMIV G'CCGG_C
Cuts at: 162 910 2159 162
Size: 748 1249 1983
Fragments arranged by size:
1983 1249 748
NspI r_CATG'y
Cuts at: 1033 1533 2440 1033
Size: 500 907 2573
Fragments arranged by size:
2573 907 500
PciI A'CATG_T
Cuts at: 2436 2436
Size: 3980
PmeI GTTT'AAAC
Cuts at: 979 979
Size: 3980
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3119 3151 3119
Size: 32 3948
PpuMI rG'GwC_Cy
Cuts at: 649 649
Size: 3980
PspOMI G'GGCC_C
Cuts at: 866 896 866
Size: 30 3950
PstI C_TGCA'G
Cuts at: 887 1027 1712 887
Size: 140 685 3155
Fragments arranged by size:
3155 685 140
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3980
PvuII CAG'CTG
Cuts at: 486 1405 1765 486
Size: 919 360 2701
Fragments arranged by size:
2701 919 360
RsrII CG'GwC_CG
Cuts at: 2175 2175
Size: 3980
SacI G_AGCT'C
Cuts at: 994 994
Size: 3980
SalI G'TCGA_C
Cuts at: 1017 1017
Size: 3980
SanDI GG'GwC_CC
Cuts at: 649 649
Size: 3980
SapI GCTCTTCn'nnn_
Cuts at: 2003 2213 2003
Size: 210 3770
SbfI CC_TGCA'GG
Cuts at: 1027 1027
Size: 3980
ScaI AGT'ACT
Cuts at: 415 3497 415
Size: 3082 898
SfcI C'TryA_G
Cuts at: 33 531 883 1023 1708 2701 2892 33
Size: 498 352 140 685 993 191 1121
Fragments arranged by size:
1121 993 685 498 352 191 140
SfoI GGC'GCC
Cuts at: 1412 1660 1412
Size: 248 3732
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3980
SmaI CCC'GGG
Cuts at: 220 1002 220
Size: 782 3198
SmlI C'TyrA_G
Cuts at: 748 1135 2050 2542 2804 748
Size: 387 915 492 262 1924
Fragments arranged by size:
1924 915 492 387 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3980
SphI G_CATG'C
Cuts at: 1033 1533 1033
Size: 500 3480
SrfI GCCC'GGGC
Cuts at: 220 220
Size: 3980
StyI C'CwwG_G
Cuts at: 755 767 1114 1468 755
Size: 12 347 354 3267
Fragments arranged by size:
3267 354 347 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1563 1696 2155 2364 1563
Size: 133 459 209 3179
Fragments arranged by size:
3179 459 209 133
TatI w'GTAC_w
Cuts at: 413 3495 413
Size: 3082 898
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3497 3529 3497
Size: 32 3948
Tth111I GACn'n_nGTC
Cuts at: 146 1777 146
Size: 1631 2349
XbaI T'CTAG_A
Cuts at: 1011 1011
Size: 3980
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 360 593 642 1475 360
Size: 233 49 833 2865
Fragments arranged by size:
2865 833 233 49
XmaI C'CCGG_G
Cuts at: 218 1000 218
Size: 782 3198
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PmeI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SrfI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BsgI BsiWI BsmI BsrGI BssHII BstEII
BstZ17I Bsu36I ClaI CspCI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmlI PshAI PsiI PspXI PsrI
SacII SexAI SfiI SgrAI SnaBI SspI StuI SwaI
XhoI XmnI ZraI