Restriction map for
pF1KE0124
(Circular) (Six-base) MAPSORT of: pF1KE0124.seq Check: 4006 from: 1 to: 4094
pF1KE0124 4094 bp
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 706 706
Size: 4094
AccI GT'mk_AC
Cuts at: 1132 1132
Size: 4094
Acc65I G'GTAC_C
Cuts at: 1110 1110
Size: 4094
AclI AA'CG_TT
Cuts at: 494 3934 494
Size: 3440 654
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 989 1919 2351 3098 3366 989
Size: 930 432 747 268 1717
Fragments arranged by size:
1717 930 747 432 268
AflIII A'CryG_T
Cuts at: 1446 2550 3527 1446
Size: 1104 977 2013
Fragments arranged by size:
2013 1104 977
AgeI A'CCGG_T
Cuts at: 2482 2482
Size: 4094
AleI CACnn'nnGTG
Cuts at: 337 337
Size: 4094
AlwNI CAG_nnn'CTG
Cuts at: 1399 2966 1399
Size: 1567 2527
ApaLI G'TGCA_C
Cuts at: 2864 2864
Size: 4094
ApoI r'AATT_y
Cuts at: 421 574 1098 3447 3703 421
Size: 153 524 2349 256 812
Fragments arranged by size:
2349 812 524 256 153
AseI AT'TA_AT
Cuts at: 20 20
Size: 4094
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4094
AvaI C'yCGr_G
Cuts at: 1114 1114
Size: 4094
BamHI G'GATC_C
Cuts at: 751 1119 751
Size: 368 3726
BanI G'GyrC_C
Cuts at: 1110 1524 1772 1807 1110
Size: 414 248 35 3397
Fragments arranged by size:
3397 414 248 35
BanII G_rGCy'C
Cuts at: 1108 2138 1108
Size: 1030 3064
BbeI G_GCGC'C
Cuts at: 1528 1776 1528
Size: 248 3846
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4060
BciVI GTATCCnnnnn_n'
Cuts at: 1992 2753 4094 1992
Size: 761 1341 1992
Fragments arranged by size:
1992 1341 761
BclI T'GATC_A
Cuts at: 1614 1614
Size: 4094
BglII A'GATC_T
Cuts at: 668 1609 3646 668
Size: 941 2037 1116
Fragments arranged by size:
2037 1116 941
BlpI GC'TnA_GC
Cuts at: 801 1206 801
Size: 405 3689
Bme1580I G_kGCm'C
Cuts at: 1719 1812 2868 1719
Size: 93 1056 2945
Fragments arranged by size:
2945 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1306 1387 1722 3866 1306
Size: 81 335 2144 1534
Fragments arranged by size:
2144 1534 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2461 2461
Size: 4094
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1270 2149 2641 2939 1270
Size: 879 492 298 2425
Fragments arranged by size:
2425 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3798 3798
Size: 4094
BsaAI yAC'GTr
Cuts at: 1447 1447
Size: 4094
BsaBI GATnn'nnATC
Cuts at: 6 1608 3645 6
Size: 1602 2037 455
Fragments arranged by size:
2037 1602 455
BsaHI Gr'CG_yC
Cuts at: 1525 1773 2475 3971 1525
Size: 248 702 1496 1648
Fragments arranged by size:
1648 1496 702 248
BsaWI w'CCGG_w
Cuts at: 194 1283 1804 2482 2756 2903 3314 194
Size: 1089 521 678 274 147 411 974
Fragments arranged by size:
1089 974 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 638 1060 638
Size: 422 3672
BseYI C'CCAG_C
Cuts at: 1519 2277 2854 3350 1519
Size: 758 577 496 2263
Fragments arranged by size:
2263 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 740 997 740
Size: 257 3837
BsiEI CG_ry'CG
Cuts at: 104 1636 1682 2890 104
Size: 1532 46 1208 1308
Fragments arranged by size:
1532 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1108 1886 2076 2868 1108
Size: 778 190 792 2334
Fragments arranged by size:
2334 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 1108 1719 1812 1886 2076 2138 2868 1108
Size: 611 93 74 190 62 730 2334
Fragments arranged by size:
2334 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 194 3314 194
Size: 3120 974
BspHI T'CATG_A
Cuts at: 3270 3270
Size: 4094
BspMI ACCTGCnnnn'nnnn_
Cuts at: 686 706 825 1144 1358 1660 2041 686
Size: 20 119 319 214 302 381 2739
Fragments arranged by size:
2739 381 319 302 214 119 20
BsrBI CCG'CTC
Cuts at: 50 361 2386 2440 3924 4089 50
Size: 311 2025 54 1484 165 55
Fragments arranged by size:
2025 1484 311 165 55 54
BsrDI GCAATG_nn'
Cuts at: 296 2006 296
Size: 1710 2384
BsrFI r'CCGG_y
Cuts at: 2092 2273 2482 2092
Size: 181 209 3704
Fragments arranged by size:
3704 209 181
BssSI C'ACGA_G
Cuts at: 973 1015 2365 2723 973
Size: 42 1350 358 2344
Fragments arranged by size:
2344 1350 358 42
BstAPI GCAn_nnn'nTGC
Cuts at: 207 1471 207
Size: 1264 2830
BstBI TT'CG_AA
Cuts at: 2455 2455
Size: 4094
BstYI r'GATC_y
Cuts at: 1 668 751 1119 1586 1609 1944 2190 3202
Size: 667 83 368 467 23 335 246 1012
Cuts at: 3202 3288 3300 3646 1
Size: 86 12 346 449
Fragments arranged by size:
1012 667 467 449 368 346 335 246
86 83 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2044 2225 2388 2044
Size: 181 163 3750
Fragments arranged by size:
3750 181 163
BtsI GCAGTG_nn'
Cuts at: 1337 1337
Size: 4094
DraI TTT'AAA
Cuts at: 326 462 1093 326
Size: 136 631 3327
Fragments arranged by size:
3327 631 136
DraIII CAC_nnn'GTG
Cuts at: 207 207
Size: 4094
DrdI GACnn_nn'nnGTC
Cuts at: 1800 2658 1800
Size: 858 3236
EaeI y'GGCC_r
Cuts at: 1679 1853 2244 2271 3616 1679
Size: 174 391 27 1345 2157
Fragments arranged by size:
2157 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1679 1679
Size: 4094
EarI CTCTTCn'nnn_
Cuts at: 394 1025 1076 2117 2327 394
Size: 631 51 1041 210 2161
Fragments arranged by size:
2161 1041 631 210 51
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2302 2612 2758 3904 2302
Size: 310 146 1146 2492
Fragments arranged by size:
2492 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1106 1106
Size: 4094
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 989 1919 2351 3098 3366 989
Size: 930 432 747 268 1717
Fragments arranged by size:
1717 930 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1233 1233
Size: 4094
EcoRI G'AATT_C
Cuts at: 1098 1098
Size: 4094
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 806 2113 806
Size: 1307 2787
FspI TGC'GCA
Cuts at: 1875 1875
Size: 4094
HaeII r_GCGC'y
Cuts at: 1528 1776 2798 3777 1528
Size: 248 1022 979 1845
Fragments arranged by size:
1845 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 278 304 310 336 1936 1966 1968 1998 278
Size: 26 6 26 1600 30 2 30 2374
Fragments arranged by size:
2374 1600 30 30 26 26 6 2
HincII GTy'rAC
Cuts at: 1133 1133
Size: 4094
KasI G'GCGC_C
Cuts at: 1524 1772 1524
Size: 248 3846
KpnI G_GTAC'C
Cuts at: 1114 1114
Size: 4094
MluI A'CGCG_T
Cuts at: 3527 3527
Size: 4094
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2765 2949 2765
Size: 184 3910
MscI TGG'CCA
Cuts at: 1855 1855
Size: 4094
MslI CAynn'nnrTG
Cuts at: 337 382 2083 2210 337
Size: 45 1701 127 2221
Fragments arranged by size:
2221 1701 127 45
MspA1I CmG'CkG
Cuts at: 1205 1519 1879 2892 3137 1205
Size: 314 360 1013 245 2162
Fragments arranged by size:
2162 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2275 2275
Size: 4094
NarI GG'CG_CC
Cuts at: 1525 1773 1525
Size: 248 3846
NgoMIV G'CCGG_C
Cuts at: 2273 2273
Size: 4094
NspI r_CATG'y
Cuts at: 387 1147 1647 2554 387
Size: 760 500 907 1927
Fragments arranged by size:
1927 907 760 500
PciI A'CATG_T
Cuts at: 2550 2550
Size: 4094
PflMI CCAn_nnn'nTGG
Cuts at: 378 378
Size: 4094
PfoI T'CCnGG_A
Cuts at: 164 539 164
Size: 375 3719
PmeI GTTT'AAAC
Cuts at: 1093 1093
Size: 4094
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3233 3265 3233
Size: 32 4062
PstI C_TGCA'G
Cuts at: 1141 1826 1141
Size: 685 3409
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4094
PvuII CAG'CTG
Cuts at: 1519 1879 1519
Size: 360 3734
RsrII CG'GwC_CG
Cuts at: 2289 2289
Size: 4094
SacI G_AGCT'C
Cuts at: 1108 1108
Size: 4094
SalI G'TCGA_C
Cuts at: 1131 1131
Size: 4094
SapI GCTCTTCn'nnn_
Cuts at: 394 1025 2117 2327 394
Size: 631 1092 210 2161
Fragments arranged by size:
2161 1092 631 210
SbfI CC_TGCA'GG
Cuts at: 1141 1141
Size: 4094
ScaI AGT'ACT
Cuts at: 3611 3611
Size: 4094
SfcI C'TryA_G
Cuts at: 33 225 614 1137 1822 2815 3006 33
Size: 192 389 523 685 993 191 1121
Fragments arranged by size:
1121 993 685 523 389 192 191
SfoI GGC'GCC
Cuts at: 1526 1774 1526
Size: 248 3846
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4094
SmaI CCC'GGG
Cuts at: 1116 1116
Size: 4094
SmlI C'TyrA_G
Cuts at: 1249 2164 2656 2918 1249
Size: 915 492 262 2425
Fragments arranged by size:
2425 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4094
SphI G_CATG'C
Cuts at: 1147 1647 1147
Size: 500 3594
StyI C'CwwG_G
Cuts at: 1228 1582 1228
Size: 354 3740
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1677 1810 2269 2478 1677
Size: 133 459 209 3293
Fragments arranged by size:
3293 459 209 133
TatI w'GTAC_w
Cuts at: 3609 3609
Size: 4094
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3611 3643 3611
Size: 32 4062
Tth111I GACn'n_nGTC
Cuts at: 1891 1891
Size: 4094
XbaI T'CTAG_A
Cuts at: 1125 1125
Size: 4094
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1589 1589
Size: 4094
XmaI C'CCGG_G
Cuts at: 1114 1114
Size: 4094
XmnI GAAnn'nnTTC
Cuts at: 449 449
Size: 4094
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BcgI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PciI PflMI PfoI
PmeI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI ApaI AscI
AvrII BaeI BbsI BbvCI BfrBI BglI BmgBI BmtI
BpmI Bpu10I BsaXI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I BtgI ClaI CspCI EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI
NheI NotI NruI NsiI PacI PasI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
ZraI