Restriction map for
pF1KE0125
(Circular) (Six-base) MAPSORT of: pF1KE0125.seq Check: 3351 from: 1 to: 3476
pF1KE0125 3476 bp
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AccI GT'mk_AC
Cuts at: 443 514 443
Size: 71 3405
Acc65I G'GTAC_C
Cuts at: 492 492
Size: 3476
AclI AA'CG_TT
Cuts at: 3316 3316
Size: 3476
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1301 1733 2480 2748 1301
Size: 432 747 268 2029
Fragments arranged by size:
2029 747 432 268
AfeI AGC'GCT
Cuts at: 217 217
Size: 3476
AflIII A'CryG_T
Cuts at: 828 1932 2909 828
Size: 1104 977 1395
Fragments arranged by size:
1395 1104 977
AgeI A'CCGG_T
Cuts at: 1864 1864
Size: 3476
AlwNI CAG_nnn'CTG
Cuts at: 781 2348 781
Size: 1567 1909
ApaLI G'TGCA_C
Cuts at: 2246 2246
Size: 3476
ApoI r'AATT_y
Cuts at: 480 2829 3085 480
Size: 2349 256 871
Fragments arranged by size:
2349 871 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3476
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3476
AvaI C'yCGr_G
Cuts at: 496 496
Size: 3476
BamHI G'GATC_C
Cuts at: 501 501
Size: 3476
BanI G'GyrC_C
Cuts at: 311 492 906 1154 1189 311
Size: 181 414 248 35 2598
Fragments arranged by size:
2598 414 248 181 35
BanII G_rGCy'C
Cuts at: 490 1520 490
Size: 1030 2446
BbeI G_GCGC'C
Cuts at: 315 910 1158 315
Size: 595 248 2633
Fragments arranged by size:
2633 595 248
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3442
BciVI GTATCCnnnnn_n'
Cuts at: 1374 2135 3476 1374
Size: 761 1341 1374
Fragments arranged by size:
1374 1341 761
BclI T'GATC_A
Cuts at: 206 996 206
Size: 790 2686
BglII A'GATC_T
Cuts at: 991 3028 991
Size: 2037 1439
BlpI GC'TnA_GC
Cuts at: 588 588
Size: 3476
Bme1580I G_kGCm'C
Cuts at: 1101 1194 2250 1101
Size: 93 1056 2327
Fragments arranged by size:
2327 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 688 769 1104 3248 688
Size: 81 335 2144 916
Fragments arranged by size:
2144 916 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1843 1843
Size: 3476
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 652 1531 2023 2321 652
Size: 879 492 298 1807
Fragments arranged by size:
1807 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3180 3180
Size: 3476
BsaAI yAC'GTr
Cuts at: 829 829
Size: 3476
BsaBI GATnn'nnATC
Cuts at: 6 990 3027 6
Size: 984 2037 455
Fragments arranged by size:
2037 984 455
BsaHI Gr'CG_yC
Cuts at: 312 907 1155 1857 3353 312
Size: 595 248 702 1496 435
Fragments arranged by size:
1496 702 595 435 248
BsaWI w'CCGG_w
Cuts at: 665 1186 1864 2138 2285 2696 665
Size: 521 678 274 147 411 1445
Fragments arranged by size:
1445 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 190 901 1659 2236 2732 190
Size: 711 758 577 496 934
Fragments arranged by size:
934 758 711 577 496
BsiEI CG_ry'CG
Cuts at: 104 153 1018 1064 2272 104
Size: 49 865 46 1208 1308
Fragments arranged by size:
1308 1208 865 49 46
BsiHKAI G_wGCw'C
Cuts at: 490 1268 1458 2250 490
Size: 778 190 792 1716
Fragments arranged by size:
1716 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 490 1101 1194 1268 1458 1520 2250 490
Size: 611 93 74 190 62 730 1716
Fragments arranged by size:
1716 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2696 2696
Size: 3476
BspHI T'CATG_A
Cuts at: 2652 2652
Size: 3476
BspMI ACCTGCnnnn'nnnn_
Cuts at: 526 740 1042 1423 526
Size: 214 302 381 2579
Fragments arranged by size:
2579 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1768 1822 3306 3471 50
Size: 1718 54 1484 165 55
Fragments arranged by size:
1718 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1388 1388
Size: 3476
BsrFI r'CCGG_y
Cuts at: 119 1474 1655 1864 119
Size: 1355 181 209 1731
Fragments arranged by size:
1731 1355 209 181
BssSI C'ACGA_G
Cuts at: 1747 2105 1747
Size: 358 3118
BstAPI GCAn_nnn'nTGC
Cuts at: 853 853
Size: 3476
BstBI TT'CG_AA
Cuts at: 1837 1837
Size: 3476
BstXI CCAn_nnnn'nTGG
Cuts at: 264 264
Size: 3476
BstYI r'GATC_y
Cuts at: 1 173 501 968 991 1326 1572 2584 2670
Size: 172 328 467 23 335 246 1012 86
Cuts at: 2670 2682 3028 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 346 335 328 246 172
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 375 1426 1607 1770 375
Size: 1051 181 163 2081
Fragments arranged by size:
2081 1051 181 163
BtsI GCAGTG_nn'
Cuts at: 719 719
Size: 3476
DraI TTT'AAA
Cuts at: 475 475
Size: 3476
DrdI GACnn_nn'nnGTC
Cuts at: 1182 2040 1182
Size: 858 2618
EaeI y'GGCC_r
Cuts at: 1061 1235 1626 1653 2998 1061
Size: 174 391 27 1345 1539
Fragments arranged by size:
1539 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1061 1061
Size: 3476
EarI CTCTTCn'nnn_
Cuts at: 1499 1709 1499
Size: 210 3266
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 153 1684 1994 2140 3286 153
Size: 1531 310 146 1146 343
Fragments arranged by size:
1531 1146 343 310 146
EcoICRI GAG'CTC
Cuts at: 488 488
Size: 3476
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1301 1733 2480 2748 1301
Size: 432 747 268 2029
Fragments arranged by size:
2029 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 615 615
Size: 3476
EcoRI G'AATT_C
Cuts at: 480 480
Size: 3476
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1495 1495
Size: 3476
FspI TGC'GCA
Cuts at: 1257 1257
Size: 3476
HaeII r_GCGC'y
Cuts at: 219 315 910 1158 2180 3159 219
Size: 96 595 248 1022 979 536
Fragments arranged by size:
1022 979 595 536 248 96
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1318 1348 1350 1380 1318
Size: 30 2 30 3414
Fragments arranged by size:
3414 30 30 2
HincII GTy'rAC
Cuts at: 282 515 282
Size: 233 3243
KasI G'GCGC_C
Cuts at: 311 906 1154 311
Size: 595 248 2633
Fragments arranged by size:
2633 595 248
KpnI G_GTAC'C
Cuts at: 496 496
Size: 3476
MluI A'CGCG_T
Cuts at: 2909 2909
Size: 3476
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2147 2331 2147
Size: 184 3292
MscI TGG'CCA
Cuts at: 1237 1237
Size: 3476
MslI CAynn'nnrTG
Cuts at: 1465 1592 1465
Size: 127 3349
MspA1I CmG'CkG
Cuts at: 135 155 237 324 587 901 1261 2274 2519
Size: 20 82 87 263 314 360 1013 245
Cuts at: 2519 135
Size: 1092
Fragments arranged by size:
1092 1013 360 314 263 245 87 82
20
NaeI GCC'GGC
Cuts at: 1657 1657
Size: 3476
NarI GG'CG_CC
Cuts at: 312 907 1155 312
Size: 595 248 2633
Fragments arranged by size:
2633 595 248
NgoMIV G'CCGG_C
Cuts at: 1655 1655
Size: 3476
NspI r_CATG'y
Cuts at: 529 1029 1936 529
Size: 500 907 2069
Fragments arranged by size:
2069 907 500
PciI A'CATG_T
Cuts at: 1932 1932
Size: 3476
PfoI T'CCnGG_A
Cuts at: 245 269 245
Size: 24 3452
PmeI GTTT'AAAC
Cuts at: 475 475
Size: 3476
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2615 2647 2615
Size: 32 3444
PstI C_TGCA'G
Cuts at: 323 523 1208 323
Size: 200 685 2591
Fragments arranged by size:
2591 685 200
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3476
PvuII CAG'CTG
Cuts at: 237 324 901 1261 237
Size: 87 577 360 2452
Fragments arranged by size:
2452 577 360 87
RsrII CG'GwC_CG
Cuts at: 1671 1671
Size: 3476
SacI G_AGCT'C
Cuts at: 490 490
Size: 3476
SalI G'TCGA_C
Cuts at: 513 513
Size: 3476
SapI GCTCTTCn'nnn_
Cuts at: 1499 1709 1499
Size: 210 3266
SbfI CC_TGCA'GG
Cuts at: 523 523
Size: 3476
ScaI AGT'ACT
Cuts at: 2993 2993
Size: 3476
SfcI C'TryA_G
Cuts at: 33 319 519 1204 2197 2388 33
Size: 286 200 685 993 191 1121
Fragments arranged by size:
1121 993 685 286 200 191
SfoI GGC'GCC
Cuts at: 313 908 1156 313
Size: 595 248 2633
Fragments arranged by size:
2633 595 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3476
SmaI CCC'GGG
Cuts at: 498 498
Size: 3476
SmlI C'TyrA_G
Cuts at: 631 1546 2038 2300 631
Size: 915 492 262 1807
Fragments arranged by size:
1807 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3476
SphI G_CATG'C
Cuts at: 529 1029 529
Size: 500 2976
StuI AGG'CCT
Cuts at: 340 340
Size: 3476
StyI C'CwwG_G
Cuts at: 610 964 610
Size: 354 3122
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1059 1192 1651 1860 1059
Size: 133 459 209 2675
Fragments arranged by size:
2675 459 209 133
TatI w'GTAC_w
Cuts at: 2991 2991
Size: 3476
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2993 3025 2993
Size: 32 3444
Tth111I GACn'n_nGTC
Cuts at: 277 1273 277
Size: 996 2480
XbaI T'CTAG_A
Cuts at: 507 507
Size: 3476
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 971 971
Size: 3476
XmaI C'CCGG_G
Cuts at: 496 496
Size: 3476
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BcgI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PciI PfoI PmeI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BfrBI BglI BmgBI
BmtI BpmI Bpu10I BsaXI BseRI BsgI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I BtgI ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI XmnI ZraI