Restriction map for
pF1KE0126
(Circular) (Six-base) MAPSORT of: pF1KE0126.seq Check: 5400 from: 1 to: 3488
pF1KE0126 3488 bp
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AccI GT'mk_AC
Cuts at: 526 526
Size: 3488
Acc65I G'GTAC_C
Cuts at: 504 504
Size: 3488
AclI AA'CG_TT
Cuts at: 3328 3328
Size: 3488
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1313 1745 2492 2760 1313
Size: 432 747 268 2041
Fragments arranged by size:
2041 747 432 268
AflIII A'CryG_T
Cuts at: 840 1944 2921 840
Size: 1104 977 1407
Fragments arranged by size:
1407 1104 977
AgeI A'CCGG_T
Cuts at: 1876 1876
Size: 3488
AlwNI CAG_nnn'CTG
Cuts at: 793 2360 793
Size: 1567 1921
ApaLI G'TGCA_C
Cuts at: 2258 2258
Size: 3488
ApoI r'AATT_y
Cuts at: 492 2841 3097 492
Size: 2349 256 883
Fragments arranged by size:
2349 883 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3488
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3488
AvaI C'yCGr_G
Cuts at: 508 508
Size: 3488
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 210 243 210
Size: 33 3455
BamHI G'GATC_C
Cuts at: 513 513
Size: 3488
BanI G'GyrC_C
Cuts at: 504 918 1166 1201 504
Size: 414 248 35 2791
Fragments arranged by size:
2791 414 248 35
BanII G_rGCy'C
Cuts at: 296 502 1532 296
Size: 206 1030 2252
Fragments arranged by size:
2252 1030 206
BbeI G_GCGC'C
Cuts at: 922 1170 922
Size: 248 3240
BbsI GAAGACnn'nnnn_
Cuts at: 127 127
Size: 3488
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3454
BciVI GTATCCnnnnn_n'
Cuts at: 1386 2147 3488 1386
Size: 761 1341 1386
Fragments arranged by size:
1386 1341 761
BclI T'GATC_A
Cuts at: 1008 1008
Size: 3488
BglII A'GATC_T
Cuts at: 1003 3040 1003
Size: 2037 1451
BlpI GC'TnA_GC
Cuts at: 600 600
Size: 3488
Bme1580I G_kGCm'C
Cuts at: 1113 1206 2262 1113
Size: 93 1056 2339
Fragments arranged by size:
2339 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 700 781 1116 3260 700
Size: 81 335 2144 928
Fragments arranged by size:
2144 928 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1855 1855
Size: 3488
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 664 1543 2035 2333 664
Size: 879 492 298 1819
Fragments arranged by size:
1819 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3192 3192
Size: 3488
BsaAI yAC'GTr
Cuts at: 841 841
Size: 3488
BsaBI GATnn'nnATC
Cuts at: 6 1002 3039 6
Size: 996 2037 455
Fragments arranged by size:
2037 996 455
BsaHI Gr'CG_yC
Cuts at: 919 1167 1869 3365 919
Size: 248 702 1496 1042
Fragments arranged by size:
1496 1042 702 248
BsaWI w'CCGG_w
Cuts at: 677 1198 1876 2150 2297 2708 677
Size: 521 678 274 147 411 1457
Fragments arranged by size:
1457 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 913 1671 2248 2744 913
Size: 758 577 496 1657
Fragments arranged by size:
1657 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1030 1076 2284 104
Size: 926 46 1208 1308
Fragments arranged by size:
1308 1208 926 46
BsiHKAI G_wGCw'C
Cuts at: 296 502 1280 1470 2262 296
Size: 206 778 190 792 1522
Fragments arranged by size:
1522 792 778 206 190
BsmBI CGTCTCn'nnnn_
Cuts at: 384 451 384
Size: 67 3421
Bsp1286I G_dGCh'C
Cuts at: 296 502 1113 1206 1280 1470 1532 2262 296
Size: 206 611 93 74 190 62 730 1522
Fragments arranged by size:
1522 730 611 206 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2708 2708
Size: 3488
BspHI T'CATG_A
Cuts at: 2664 2664
Size: 3488
BspMI ACCTGCnnnn'nnnn_
Cuts at: 538 752 1054 1435 538
Size: 214 302 381 2591
Fragments arranged by size:
2591 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1780 1834 3318 3483 50
Size: 1730 54 1484 165 55
Fragments arranged by size:
1730 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1400 1400
Size: 3488
BsrFI r'CCGG_y
Cuts at: 1486 1667 1876 1486
Size: 181 209 3098
Fragments arranged by size:
3098 209 181
BsrGI T'GTAC_A
Cuts at: 434 434
Size: 3488
BssSI C'ACGA_G
Cuts at: 1759 2117 1759
Size: 358 3130
BstAPI GCAn_nnn'nTGC
Cuts at: 865 865
Size: 3488
BstBI TT'CG_AA
Cuts at: 146 1849 146
Size: 1703 1785
BstYI r'GATC_y
Cuts at: 1 513 980 1003 1338 1584 2596 2682 2694
Size: 512 467 23 335 246 1012 86 12
Cuts at: 2694 3040 1
Size: 346 449
Fragments arranged by size:
1012 512 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1438 1619 1782 1438
Size: 181 163 3144
Fragments arranged by size:
3144 181 163
BtsI GCAGTG_nn'
Cuts at: 731 731
Size: 3488
DraI TTT'AAA
Cuts at: 487 487
Size: 3488
DrdI GACnn_nn'nnGTC
Cuts at: 1194 2052 1194
Size: 858 2630
EaeI y'GGCC_r
Cuts at: 1073 1247 1638 1665 3010 1073
Size: 174 391 27 1345 1551
Fragments arranged by size:
1551 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1073 1073
Size: 3488
EarI CTCTTCn'nnn_
Cuts at: 1511 1721 1511
Size: 210 3278
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1696 2006 2152 3298 1696
Size: 310 146 1146 1886
Fragments arranged by size:
1886 1146 310 146
EcoICRI GAG'CTC
Cuts at: 294 500 294
Size: 206 3282
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1313 1745 2492 2760 1313
Size: 432 747 268 2041
Fragments arranged by size:
2041 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 627 627
Size: 3488
EcoRI G'AATT_C
Cuts at: 492 492
Size: 3488
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1507 1507
Size: 3488
FspI TGC'GCA
Cuts at: 1269 1269
Size: 3488
HaeII r_GCGC'y
Cuts at: 138 318 922 1170 2192 3171 138
Size: 180 604 248 1022 979 455
Fragments arranged by size:
1022 979 604 455 248 180
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1330 1360 1362 1392 1330
Size: 30 2 30 3426
Fragments arranged by size:
3426 30 30 2
HincII GTy'rAC
Cuts at: 282 527 282
Size: 245 3243
KasI G'GCGC_C
Cuts at: 918 1166 918
Size: 248 3240
KpnI G_GTAC'C
Cuts at: 508 508
Size: 3488
MluI A'CGCG_T
Cuts at: 2921 2921
Size: 3488
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 95 192 214 2159 2343 95
Size: 97 22 1945 184 1240
Fragments arranged by size:
1945 1240 184 97 22
MscI TGG'CCA
Cuts at: 1249 1249
Size: 3488
MslI CAynn'nnrTG
Cuts at: 1477 1604 1477
Size: 127 3361
MspA1I CmG'CkG
Cuts at: 599 913 1273 2286 2531 599
Size: 314 360 1013 245 1556
Fragments arranged by size:
1556 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1669 1669
Size: 3488
NarI GG'CG_CC
Cuts at: 919 1167 919
Size: 248 3240
NgoMIV G'CCGG_C
Cuts at: 1667 1667
Size: 3488
NspI r_CATG'y
Cuts at: 541 1041 1948 541
Size: 500 907 2081
Fragments arranged by size:
2081 907 500
PciI A'CATG_T
Cuts at: 1944 1944
Size: 3488
PmeI GTTT'AAAC
Cuts at: 487 487
Size: 3488
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2627 2659 2627
Size: 32 3456
PstI C_TGCA'G
Cuts at: 535 1220 535
Size: 685 2803
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3488
PvuII CAG'CTG
Cuts at: 913 1273 913
Size: 360 3128
RsrII CG'GwC_CG
Cuts at: 1683 1683
Size: 3488
SacI G_AGCT'C
Cuts at: 296 502 296
Size: 206 3282
SalI G'TCGA_C
Cuts at: 525 525
Size: 3488
SapI GCTCTTCn'nnn_
Cuts at: 1511 1721 1511
Size: 210 3278
SbfI CC_TGCA'GG
Cuts at: 535 535
Size: 3488
ScaI AGT'ACT
Cuts at: 3005 3005
Size: 3488
SfcI C'TryA_G
Cuts at: 33 531 1216 2209 2400 33
Size: 498 685 993 191 1121
Fragments arranged by size:
1121 993 685 498 191
SfoI GGC'GCC
Cuts at: 920 1168 920
Size: 248 3240
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3488
SmaI CCC'GGG
Cuts at: 510 510
Size: 3488
SmlI C'TyrA_G
Cuts at: 643 1558 2050 2312 643
Size: 915 492 262 1819
Fragments arranged by size:
1819 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3488
SphI G_CATG'C
Cuts at: 541 1041 541
Size: 500 2988
StuI AGG'CCT
Cuts at: 340 340
Size: 3488
StyI C'CwwG_G
Cuts at: 622 976 622
Size: 354 3134
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1071 1204 1663 1872 1071
Size: 133 459 209 2687
Fragments arranged by size:
2687 459 209 133
TatI w'GTAC_w
Cuts at: 434 3003 434
Size: 2569 919
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3005 3037 3005
Size: 32 3456
Tth111I GACn'n_nGTC
Cuts at: 277 1285 277
Size: 1008 2480
XbaI T'CTAG_A
Cuts at: 519 519
Size: 3488
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 983 983
Size: 3488
XmaI C'CCGG_G
Cuts at: 508 508
Size: 3488
XmnI GAAnn'nnTTC
Cuts at: 124 124
Size: 3488
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PciI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BbvCI BfrBI BglI BmgBI BmtI
BpmI Bpu10I BsaXI BseRI BsgI BsiWI BsmI BssHII
BstEII BstXI BstZ17I Bsu36I BtgI ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI ZraI