Restriction map for
pF1KE0127
(Circular) (Six-base) MAPSORT of: pF1KE0127.seq Check: 2129 from: 1 to: 4241
pF1KE0127 4241 bp
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AccI GT'mk_AC
Cuts at: 1279 1279
Size: 4241
Acc65I G'GTAC_C
Cuts at: 113 1257 113
Size: 1144 3097
AclI AA'CG_TT
Cuts at: 4081 4081
Size: 4241
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 130 178 658 764 2066 2498 3245 3513 130
Size: 48 480 106 1302 432 747 268 858
Fragments arranged by size:
1302 858 747 480 432 268 106 48
AflIII A'CryG_T
Cuts at: 1593 2697 3674 1593
Size: 1104 977 2160
Fragments arranged by size:
2160 1104 977
AgeI A'CCGG_T
Cuts at: 2629 2629
Size: 4241
AhdI GACnn_n'nnGTC
Cuts at: 434 1187 434
Size: 753 3488
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 931 931
Size: 4241
AlwNI CAG_nnn'CTG
Cuts at: 1546 3113 1546
Size: 1567 2674
ApaLI G'TGCA_C
Cuts at: 3011 3011
Size: 4241
ApoI r'AATT_y
Cuts at: 970 1245 3594 3850 970
Size: 275 2349 256 1361
Fragments arranged by size:
2349 1361 275 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4241
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4241
AvaI C'yCGr_G
Cuts at: 1261 1261
Size: 4241
BamHI G'GATC_C
Cuts at: 1266 1266
Size: 4241
BanI G'GyrC_C
Cuts at: 113 230 960 1257 1671 1919 1954 113
Size: 117 730 297 414 248 35 2400
Fragments arranged by size:
2400 730 414 297 248 117 35
BanII G_rGCy'C
Cuts at: 860 1255 2285 860
Size: 395 1030 2816
Fragments arranged by size:
2816 1030 395
BbeI G_GCGC'C
Cuts at: 1675 1923 1675
Size: 248 3993
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 326 360 326
Size: 34 4207
BciVI GTATCCnnnnn_n'
Cuts at: 387 2139 2900 4241 387
Size: 1752 761 1341 387
Fragments arranged by size:
1752 1341 761 387
BclI T'GATC_A
Cuts at: 1097 1761 1097
Size: 664 3577
BglII A'GATC_T
Cuts at: 1756 3793 1756
Size: 2037 2204
BlpI GC'TnA_GC
Cuts at: 351 1353 351
Size: 1002 3239
Bme1580I G_kGCm'C
Cuts at: 1866 1959 3015 1866
Size: 93 1056 3092
Fragments arranged by size:
3092 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1453 1534 1869 4013 1453
Size: 81 335 2144 1681
Fragments arranged by size:
2144 1681 335 81
BmtI G_CTAG'C
Cuts at: 820 820
Size: 4241
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2608 2608
Size: 4241
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 774 774
Size: 4241
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 686 1417 2296 2788 3086 686
Size: 731 879 492 298 1841
Fragments arranged by size:
1841 879 731 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 332 439 3945 332
Size: 107 3506 628
Fragments arranged by size:
3506 628 107
BsaAI yAC'GTr
Cuts at: 1594 1594
Size: 4241
BsaBI GATnn'nnATC
Cuts at: 6 1755 3792 6
Size: 1749 2037 455
Fragments arranged by size:
2037 1749 455
BsaHI Gr'CG_yC
Cuts at: 1672 1920 2622 4118 1672
Size: 248 702 1496 1795
Fragments arranged by size:
1795 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1430 1951 2629 2903 3050 3461 1430
Size: 521 678 274 147 411 2210
Fragments arranged by size:
2210 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 179 970 1137 179
Size: 791 167 3283
Fragments arranged by size:
3283 791 167
BseYI C'CCAG_C
Cuts at: 1666 2424 3001 3497 1666
Size: 758 577 496 2410
Fragments arranged by size:
2410 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1783 1829 3037 104
Size: 1679 46 1208 1308
Fragments arranged by size:
1679 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 710 1255 2033 2223 3015 710
Size: 545 778 190 792 1936
Fragments arranged by size:
1936 792 778 545 190
BsmI GAATG_Cn'
Cuts at: 570 676 866 570
Size: 106 190 3945
Fragments arranged by size:
3945 190 106
BsmBI CGTCTCn'nnnn_
Cuts at: 1203 1203
Size: 4241
Bsp1286I G_dGCh'C
Cuts at: 710 860 1255 1866 1959 2033 2223 2285 3015
Size: 150 395 611 93 74 190 62 730
Cuts at: 3015 710
Size: 1936
Fragments arranged by size:
1936 730 611 395 190 150 93 74
62
BspEI T'CCGG_A
Cuts at: 3461 3461
Size: 4241
BspHI T'CATG_A
Cuts at: 3417 3417
Size: 4241
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1291 1505 1807 2188 1291
Size: 214 302 381 3344
Fragments arranged by size:
3344 381 302 214
BsrBI CCG'CTC
Cuts at: 50 350 2533 2587 4071 4236 50
Size: 300 2183 54 1484 165 55
Fragments arranged by size:
2183 1484 300 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2153 2153
Size: 4241
BsrFI r'CCGG_y
Cuts at: 2239 2420 2629 2239
Size: 181 209 3851
Fragments arranged by size:
3851 209 181
BssSI C'ACGA_G
Cuts at: 2512 2870 2512
Size: 358 3883
BstAPI GCAn_nnn'nTGC
Cuts at: 1618 1618
Size: 4241
BstBI TT'CG_AA
Cuts at: 2602 2602
Size: 4241
BstXI CCAn_nnnn'nTGG
Cuts at: 438 1045 438
Size: 607 3634
BstYI r'GATC_y
Cuts at: 1 1266 1733 1756 2091 2337 3349 3435 3447
Size: 1265 467 23 335 246 1012 86 12
Cuts at: 3447 3793 1
Size: 346 449
Fragments arranged by size:
1265 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 965 965
Size: 4241
BtgI C'CryG_G
Cuts at: 1075 1075
Size: 4241
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2191 2372 2535 2191
Size: 181 163 3897
Fragments arranged by size:
3897 181 163
BtsI GCAGTG_nn'
Cuts at: 342 800 913 1144 1484 342
Size: 458 113 231 340 3099
Fragments arranged by size:
3099 458 340 231 113
DraI TTT'AAA
Cuts at: 1240 1240
Size: 4241
DrdI GACnn_nn'nnGTC
Cuts at: 1947 2805 1947
Size: 858 3383
EaeI y'GGCC_r
Cuts at: 466 1826 2000 2391 2418 3763 466
Size: 1360 174 391 27 1345 944
Fragments arranged by size:
1360 1345 944 391 174 27
EagI C'GGCC_G
Cuts at: 1826 1826
Size: 4241
EarI CTCTTCn'nnn_
Cuts at: 2264 2474 2264
Size: 210 4031
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2449 2759 2905 4051 2449
Size: 310 146 1146 2639
Fragments arranged by size:
2639 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1253 1253
Size: 4241
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 130 178 658 764 774 2066 2498 3245 3513
Size: 48 480 106 10 1292 432 747 268
Cuts at: 3513 130
Size: 858
Fragments arranged by size:
1292 858 747 480 432 268 106 48
10
EcoO109I rG'GnC_Cy
Cuts at: 1380 1380
Size: 4241
EcoRI G'AATT_C
Cuts at: 1245 1245
Size: 4241
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2260 2260
Size: 4241
FspI TGC'GCA
Cuts at: 2022 2022
Size: 4241
HaeII r_GCGC'y
Cuts at: 1675 1923 2945 3924 1675
Size: 248 1022 979 1992
Fragments arranged by size:
1992 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 420 452 1173 1205 2083 2113 2115 2145 420
Size: 32 721 32 878 30 2 30 2516
Fragments arranged by size:
2516 878 721 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1131 1280 1131
Size: 149 4092
HindIII A'AGCT_T
Cuts at: 204 204
Size: 4241
KasI G'GCGC_C
Cuts at: 1671 1919 1671
Size: 248 3993
KpnI G_GTAC'C
Cuts at: 117 1261 117
Size: 1144 3097
MluI A'CGCG_T
Cuts at: 3674 3674
Size: 4241
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1002 2912 3096 1002
Size: 1910 184 2147
Fragments arranged by size:
2147 1910 184
MscI TGG'CCA
Cuts at: 468 2002 468
Size: 1534 2707
MslI CAynn'nnrTG
Cuts at: 871 1155 2230 2357 871
Size: 284 1075 127 2755
Fragments arranged by size:
2755 1075 284 127
MspA1I CmG'CkG
Cuts at: 1352 1666 2026 3039 3284 1352
Size: 314 360 1013 245 2309
Fragments arranged by size:
2309 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2422 2422
Size: 4241
NarI GG'CG_CC
Cuts at: 1672 1920 1672
Size: 248 3993
NcoI C'CATG_G
Cuts at: 1075 1075
Size: 4241
NgoMIV G'CCGG_C
Cuts at: 2420 2420
Size: 4241
NheI G'CTAG_C
Cuts at: 816 816
Size: 4241
NspI r_CATG'y
Cuts at: 1294 1794 2701 1294
Size: 500 907 2834
Fragments arranged by size:
2834 907 500
PciI A'CATG_T
Cuts at: 2697 2697
Size: 4241
PmeI GTTT'AAAC
Cuts at: 1240 1240
Size: 4241
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3380 3412 3380
Size: 32 4209
PstI C_TGCA'G
Cuts at: 1288 1973 1288
Size: 685 3556
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4241
PvuII CAG'CTG
Cuts at: 1666 2026 1666
Size: 360 3881
RsrII CG'GwC_CG
Cuts at: 2436 2436
Size: 4241
SacI G_AGCT'C
Cuts at: 1255 1255
Size: 4241
SalI G'TCGA_C
Cuts at: 1278 1278
Size: 4241
SapI GCTCTTCn'nnn_
Cuts at: 2264 2474 2264
Size: 210 4031
SbfI CC_TGCA'GG
Cuts at: 1288 1288
Size: 4241
ScaI AGT'ACT
Cuts at: 3758 3758
Size: 4241
SfcI C'TryA_G
Cuts at: 33 1284 1969 2962 3153 33
Size: 1251 685 993 191 1121
Fragments arranged by size:
1251 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1673 1921 1673
Size: 248 3993
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4241
SmaI CCC'GGG
Cuts at: 1263 1263
Size: 4241
SmlI C'TyrA_G
Cuts at: 701 1396 2311 2803 3065 701
Size: 695 915 492 262 1877
Fragments arranged by size:
1877 915 695 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4241
SphI G_CATG'C
Cuts at: 1294 1794 1294
Size: 500 3741
SspI AAT'ATT
Cuts at: 1178 1178
Size: 4241
StyI C'CwwG_G
Cuts at: 1075 1375 1729 1075
Size: 300 354 3587
Fragments arranged by size:
3587 354 300
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1824 1957 2416 2625 1824
Size: 133 459 209 3440
Fragments arranged by size:
3440 459 209 133
TatI w'GTAC_w
Cuts at: 3756 3756
Size: 4241
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 109 141 3758 3790 109
Size: 32 3617 32 560
Fragments arranged by size:
3617 560 32 32
Tth111I GACn'n_nGTC
Cuts at: 2038 2038
Size: 4241
XbaI T'CTAG_A
Cuts at: 1272 1272
Size: 4241
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 819 1736 819
Size: 917 3324
XmaI C'CCGG_G
Cuts at: 1261 1261
Size: 4241
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BcgI BciVI BclI BglII BlpI Bme1580I
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NheI NspI PciI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AloI ApaI AscI
AvrII BaeI BbsI BbvCI BfrBI BglI BmgBI Bpu10I
BsaXI BsgI BsiWI BsrGI BssHII BstEII BstZ17I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HpaI MfeI
NdeI NotI NruI NsiI PacI PasI PflMI PfoI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI StuI SwaI
XhoI XmnI ZraI