Restriction map for
pF1KE0130
(Circular) (Six-base) MAPSORT of: pF1KE0130.seq Check: 2755 from: 1 to: 4310
pF1KE0130 4310 bp
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AccI GT'mk_AC
Cuts at: 1348 1348
Size: 4310
Acc65I G'GTAC_C
Cuts at: 1326 1326
Size: 4310
AclI AA'CG_TT
Cuts at: 1304 4150 1304
Size: 2846 1464
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2135 2567 3314 3582 2135
Size: 432 747 268 2863
Fragments arranged by size:
2863 747 432 268
AflIII A'CryG_T
Cuts at: 1662 2766 3743 1662
Size: 1104 977 2229
Fragments arranged by size:
2229 1104 977
AgeI A'CCGG_T
Cuts at: 2698 2698
Size: 4310
AhdI GACnn_n'nnGTC
Cuts at: 813 813
Size: 4310
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 184 184
Size: 4310
AlwNI CAG_nnn'CTG
Cuts at: 570 1615 3182 570
Size: 1045 1567 1698
Fragments arranged by size:
1698 1567 1045
ApaI G_GGCC'C
Cuts at: 370 987 370
Size: 617 3693
ApaLI G'TGCA_C
Cuts at: 733 3080 733
Size: 2347 1963
ApoI r'AATT_y
Cuts at: 208 1314 3663 3919 208
Size: 1106 2349 256 599
Fragments arranged by size:
2349 1106 599 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4310
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4310
AvaI C'yCGr_G
Cuts at: 223 1145 1330 223
Size: 922 185 3203
Fragments arranged by size:
3203 922 185
BamHI G'GATC_C
Cuts at: 1335 1335
Size: 4310
BanI G'GyrC_C
Cuts at: 134 478 560 1141 1153 1326 1740 1988 2023
Size: 344 82 581 12 173 414 248 35
Cuts at: 2023 134
Size: 2421
Fragments arranged by size:
2421 581 414 344 248 173 82 35
12
BanII G_rGCy'C
Cuts at: 314 370 987 1070 1122 1262 1277 1324 2354
Size: 56 617 83 52 140 15 47 1030
Cuts at: 2354 314
Size: 2270
Fragments arranged by size:
2270 1030 617 140 83 56 52 47
15
BbeI G_GCGC'C
Cuts at: 482 1744 1992 482
Size: 1262 248 2800
Fragments arranged by size:
2800 1262 248
BbvCI CC'TCA_GC
Cuts at: 1253 1253
Size: 4310
BciVI GTATCCnnnnn_n'
Cuts at: 2208 2969 4310 2208
Size: 761 1341 2208
Fragments arranged by size:
2208 1341 761
BclI T'GATC_A
Cuts at: 1830 1830
Size: 4310
BglI GCCn_nnn'nGGC
Cuts at: 298 766 298
Size: 468 3842
BglII A'GATC_T
Cuts at: 1825 3862 1825
Size: 2037 2273
BlpI GC'TnA_GC
Cuts at: 851 1422 851
Size: 571 3739
Bme1580I G_kGCm'C
Cuts at: 370 392 737 987 1146 1158 1935 2028 3084
Size: 22 345 250 159 12 777 93 1056
Cuts at: 3084 370
Size: 1596
Fragments arranged by size:
1596 1056 777 345 250 159 93 22
12
BmrI ACTGGGnnnn_n'
Cuts at: 371 1522 1603 1938 4082 371
Size: 1151 81 335 2144 599
Fragments arranged by size:
2144 1151 599 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 891 2677 891
Size: 1786 2524
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1216 1249 1309 1216
Size: 33 60 4217
Fragments arranged by size:
4217 60 33
Bpu10I CC'TnA_GC
Cuts at: 399 1253 399
Size: 854 3456
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1261 1486 2365 2857 3155 1261
Size: 225 879 492 298 2416
Fragments arranged by size:
2416 879 492 298 225
BsaI GGTCTCn'nnnn_
Cuts at: 424 675 1046 4014 424
Size: 251 371 2968 720
Fragments arranged by size:
2968 720 371 251
BsaAI yAC'GTr
Cuts at: 1663 1663
Size: 4310
BsaBI GATnn'nnATC
Cuts at: 6 1824 3861 6
Size: 1818 2037 455
Fragments arranged by size:
2037 1818 455
BsaHI Gr'CG_yC
Cuts at: 479 1741 1989 2691 4187 479
Size: 1262 248 702 1496 602
Fragments arranged by size:
1496 1262 702 602 248
BsaWI w'CCGG_w
Cuts at: 1499 2020 2698 2972 3119 3530 1499
Size: 521 678 274 147 411 2279
Fragments arranged by size:
2279 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 295 421 625 699 702 705 1219 1252 1270
Size: 126 204 74 3 3 514 33 18
Cuts at: 1270 295
Size: 3335
Fragments arranged by size:
3335 514 204 126 74 33 18 3
3
BseYI C'CCAG_C
Cuts at: 1062 1735 2493 3070 3566 1062
Size: 673 758 577 496 1806
Fragments arranged by size:
1806 758 673 577 496
BsiEI CG_ry'CG
Cuts at: 104 318 426 954 1852 1898 3106 104
Size: 214 108 528 898 46 1208 1308
Fragments arranged by size:
1308 1208 898 528 214 108 46
BsiHKAI G_wGCw'C
Cuts at: 314 737 1262 1324 2102 2292 3084 314
Size: 423 525 62 778 190 792 1540
Fragments arranged by size:
1540 792 778 525 423 190 62
BsmBI CGTCTCn'nnnn_
Cuts at: 877 877
Size: 4310
Bsp1286I G_dGCh'C
Cuts at: 314 370 392 737 987 1070 1122 1146 1158
Size: 56 22 345 250 83 52 24 12
Cuts at: 1158 1262 1277 1324 1935 2028 2102 2292 2354
Size: 104 15 47 611 93 74 190 62
Cuts at: 2354 3084 314
Size: 730 1540
Fragments arranged by size:
1540 730 611 345 250 190 104 93
83 74 62 56 52 47 24 22
15 12
BspEI T'CCGG_A
Cuts at: 3530 3530
Size: 4310
BspHI T'CATG_A
Cuts at: 3486 3486
Size: 4310
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1360 1574 1876 2257 1360
Size: 214 302 381 3413
Fragments arranged by size:
3413 381 302 214
BsrBI CCG'CTC
Cuts at: 50 850 2602 2656 4140 4305 50
Size: 800 1752 54 1484 165 55
Fragments arranged by size:
1752 1484 800 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2222 2222
Size: 4310
BsrFI r'CCGG_y
Cuts at: 137 475 1138 2308 2489 2698 137
Size: 338 663 1170 181 209 1749
Fragments arranged by size:
1749 1170 663 338 209 181
BssSI C'ACGA_G
Cuts at: 730 2581 2939 730
Size: 1851 358 2101
Fragments arranged by size:
2101 1851 358
BstAPI GCAn_nnn'nTGC
Cuts at: 506 732 1687 506
Size: 226 955 3129
Fragments arranged by size:
3129 955 226
BstBI TT'CG_AA
Cuts at: 2671 2671
Size: 4310
BstEII G'GTnAC_C
Cuts at: 114 114
Size: 4310
BstXI CCAn_nnnn'nTGG
Cuts at: 802 802
Size: 4310
BstYI r'GATC_y
Cuts at: 1 1335 1802 1825 2160 2406 3418 3504 3516
Size: 1334 467 23 335 246 1012 86 12
Cuts at: 3516 3862 1
Size: 346 449
Fragments arranged by size:
1334 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 235 964 235
Size: 729 3581
BtgI C'CryG_G
Cuts at: 107 296 370 917 107
Size: 189 74 547 3500
Fragments arranged by size:
3500 547 189 74
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2260 2441 2604 2260
Size: 181 163 3966
Fragments arranged by size:
3966 181 163
BtsI GCAGTG_nn'
Cuts at: 1553 1553
Size: 4310
DraI TTT'AAA
Cuts at: 1309 1309
Size: 4310
DrdI GACnn_nn'nnGTC
Cuts at: 322 2016 2874 322
Size: 1694 858 1758
Fragments arranged by size:
1758 1694 858
EaeI y'GGCC_r
Cuts at: 473 935 1031 1895 2069 2460 2487 3832 473
Size: 462 96 864 174 391 27 1345 951
Fragments arranged by size:
1345 951 864 462 391 174 96 27
EagI C'GGCC_G
Cuts at: 1895 1895
Size: 4310
EarI CTCTTCn'nnn_
Cuts at: 2333 2543 2333
Size: 210 4100
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 763 936 2518 2828 2974 4120 763
Size: 173 1582 310 146 1146 953
Fragments arranged by size:
1582 1146 953 310 173 146
EcoICRI GAG'CTC
Cuts at: 312 1260 1322 312
Size: 948 62 3300
Fragments arranged by size:
3300 948 62
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1216 1249 1309 2135 2567 3314 3582 1216
Size: 33 60 826 432 747 268 1944
Fragments arranged by size:
1944 826 747 432 268 60 33
EcoNI CCTnn'n_nnAGG
Cuts at: 793 1231 1267 793
Size: 438 36 3836
Fragments arranged by size:
3836 438 36
EcoO109I rG'GnC_Cy
Cuts at: 165 366 930 983 1204 1224 1449 165
Size: 201 564 53 221 20 225 3026
Fragments arranged by size:
3026 564 225 221 201 53 20
EcoRI G'AATT_C
Cuts at: 208 1314 208
Size: 1106 3204
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2329 2329
Size: 4310
FspI TGC'GCA
Cuts at: 499 2091 499
Size: 1592 2718
HaeII r_GCGC'y
Cuts at: 482 1744 1992 3014 3993 482
Size: 1262 248 1022 979 799
Fragments arranged by size:
1262 1022 979 799 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 799 831 2152 2182 2184 2214 799
Size: 32 1321 30 2 30 2895
Fragments arranged by size:
2895 1321 32 30 30 2
HincII GTy'rAC
Cuts at: 153 327 1349 153
Size: 174 1022 3114
Fragments arranged by size:
3114 1022 174
KasI G'GCGC_C
Cuts at: 478 1740 1988 478
Size: 1262 248 2800
Fragments arranged by size:
2800 1262 248
KpnI G_GTAC'C
Cuts at: 1330 1330
Size: 4310
MluI A'CGCG_T
Cuts at: 3743 3743
Size: 4310
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2981 3165 2981
Size: 184 4126
MscI TGG'CCA
Cuts at: 937 2071 937
Size: 1134 3176
MslI CAynn'nnrTG
Cuts at: 112 646 832 2299 2426 112
Size: 534 186 1467 127 1996
Fragments arranged by size:
1996 1467 534 186 127
MspA1I CmG'CkG
Cuts at: 298 1421 1735 2095 3108 3353 298
Size: 1123 314 360 1013 245 1255
Fragments arranged by size:
1255 1123 1013 360 314 245
NaeI GCC'GGC
Cuts at: 477 2491 477
Size: 2014 2296
NarI GG'CG_CC
Cuts at: 479 1741 1989 479
Size: 1262 248 2800
Fragments arranged by size:
2800 1262 248
NcoI C'CATG_G
Cuts at: 107 917 107
Size: 810 3500
NgoMIV G'CCGG_C
Cuts at: 475 2489 475
Size: 2014 2296
NspI r_CATG'y
Cuts at: 658 1363 1863 2770 658
Size: 705 500 907 2198
Fragments arranged by size:
2198 907 705 500
PasI CC'CwG_GG
Cuts at: 344 483 542 604 344
Size: 139 59 62 4050
Fragments arranged by size:
4050 139 62 59
PciI A'CATG_T
Cuts at: 2766 2766
Size: 4310
PflMI CCAn_nnn'nTGG
Cuts at: 570 570
Size: 4310
PfoI T'CCnGG_A
Cuts at: 185 440 583 1265 1286 185
Size: 255 143 682 21 3209
Fragments arranged by size:
3209 682 255 143 21
PmeI GTTT'AAAC
Cuts at: 1309 1309
Size: 4310
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3449 3481 3449
Size: 32 4278
PpuMI rG'GwC_Cy
Cuts at: 1204 1204
Size: 4310
PspOMI G'GGCC_C
Cuts at: 366 983 366
Size: 617 3693
PspXI vC'TCGA_Gb
Cuts at: 223 223
Size: 4310
PstI C_TGCA'G
Cuts at: 128 728 911 1357 2042 128
Size: 600 183 446 685 2396
Fragments arranged by size:
2396 685 600 446 183
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4310
PvuII CAG'CTG
Cuts at: 1735 2095 1735
Size: 360 3950
RsrII CG'GwC_CG
Cuts at: 2505 2505
Size: 4310
SacI G_AGCT'C
Cuts at: 314 1262 1324 314
Size: 948 62 3300
Fragments arranged by size:
3300 948 62
SacII CC_GC'GG
Cuts at: 299 299
Size: 4310
SalI G'TCGA_C
Cuts at: 1347 1347
Size: 4310
SapI GCTCTTCn'nnn_
Cuts at: 2333 2543 2333
Size: 210 4100
SbfI CC_TGCA'GG
Cuts at: 1357 1357
Size: 4310
ScaI AGT'ACT
Cuts at: 3827 3827
Size: 4310
SfcI C'TryA_G
Cuts at: 33 124 724 907 1353 2038 3031 3222 33
Size: 91 600 183 446 685 993 191 1121
Fragments arranged by size:
1121 993 685 600 446 191 183 91
SfoI GGC'GCC
Cuts at: 480 1742 1990 480
Size: 1262 248 2800
Fragments arranged by size:
2800 1262 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4310
SmaI CCC'GGG
Cuts at: 1147 1332 1147
Size: 185 4125
SmlI C'TyrA_G
Cuts at: 223 1276 1465 2380 2872 3134 223
Size: 1053 189 915 492 262 1399
Fragments arranged by size:
1399 1053 915 492 262 189
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4310
SphI G_CATG'C
Cuts at: 1363 1863 1363
Size: 500 3810
SrfI GCCC'GGGC
Cuts at: 1147 1147
Size: 4310
StuI AGG'CCT
Cuts at: 240 240
Size: 4310
StyI C'CwwG_G
Cuts at: 107 917 1444 1798 107
Size: 810 527 354 2619
Fragments arranged by size:
2619 810 527 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 406 412 475 514 534 1893 2026 2485 2694
Size: 6 63 39 20 1359 133 459 209
Cuts at: 2694 406
Size: 2022
Fragments arranged by size:
2022 1359 459 209 133 63 39 20
6
TatI w'GTAC_w
Cuts at: 3825 3825
Size: 4310
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3827 3859 3827
Size: 32 4278
Tth111I GACn'n_nGTC
Cuts at: 2107 2107
Size: 4310
XbaI T'CTAG_A
Cuts at: 1341 1341
Size: 4310
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 300 1805 300
Size: 1505 2805
XhoI C'TCGA_G
Cuts at: 223 223
Size: 4310
XmaI C'CCGG_G
Cuts at: 1145 1330 1145
Size: 185 4125
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PspOMI PspXI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SrfI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AloI AscI AvrII
BaeI BbsI BcgI BfrBI BmgBI BmtI BsaXI BsgI
BsiWI BsmI BsrGI BssHII BstZ17I ClaI CspCI DraIII
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PmlI PshAI PsiI PsrI
SanDI SexAI SfiI SgrAI SnaBI SspI SwaI XmnI
ZraI