Restriction map for
pF1KE0133
(Circular) (Six-base) MAPSORT of: pF1KE0133.seq Check: 8448 from: 1 to: 3896
pF1KE0133 3896 bp
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AccI GT'mk_AC
Cuts at: 934 934
Size: 3896
Acc65I G'GTAC_C
Cuts at: 912 912
Size: 3896
AclI AA'CG_TT
Cuts at: 3736 3736
Size: 3896
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 382 1721 2153 2900 3168 382
Size: 1339 432 747 268 1110
Fragments arranged by size:
1339 1110 747 432 268
AflIII A'CryG_T
Cuts at: 368 886 1248 2352 3329 368
Size: 518 362 1104 977 935
Fragments arranged by size:
1104 977 935 518 362
AgeI A'CCGG_T
Cuts at: 2284 2284
Size: 3896
AlwNI CAG_nnn'CTG
Cuts at: 411 642 1201 2768 411
Size: 231 559 1567 1539
Fragments arranged by size:
1567 1539 559 231
ApaLI G'TGCA_C
Cuts at: 136 2666 136
Size: 2530 1366
ApoI r'AATT_y
Cuts at: 900 3249 3505 900
Size: 2349 256 1291
Fragments arranged by size:
2349 1291 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3896
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3896
AvaI C'yCGr_G
Cuts at: 916 916
Size: 3896
BamHI G'GATC_C
Cuts at: 921 921
Size: 3896
BanI G'GyrC_C
Cuts at: 234 431 494 912 1326 1574 1609 234
Size: 197 63 418 414 248 35 2521
Fragments arranged by size:
2521 418 414 248 197 63 35
BanII G_rGCy'C
Cuts at: 910 1940 910
Size: 1030 2866
BbeI G_GCGC'C
Cuts at: 238 435 1330 1578 238
Size: 197 895 248 2556
Fragments arranged by size:
2556 895 248 197
BbsI GAAGACnn'nnnn_
Cuts at: 889 889
Size: 3896
BciVI GTATCCnnnnn_n'
Cuts at: 1794 2555 3896 1794
Size: 761 1341 1794
Fragments arranged by size:
1794 1341 761
BclI T'GATC_A
Cuts at: 1416 1416
Size: 3896
BglI GCCn_nnn'nGGC
Cuts at: 262 779 262
Size: 517 3379
BglII A'GATC_T
Cuts at: 1411 3448 1411
Size: 2037 1859
BlpI GC'TnA_GC
Cuts at: 1008 1008
Size: 3896
Bme1580I G_kGCm'C
Cuts at: 140 1521 1614 2670 140
Size: 1381 93 1056 1366
Fragments arranged by size:
1381 1366 1056 93
BmgBI CAC'GTC
Cuts at: 369 807 369
Size: 438 3458
BmrI ACTGGGnnnn_n'
Cuts at: 616 1108 1189 1524 3668 616
Size: 492 81 335 2144 844
Fragments arranged by size:
2144 844 492 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 567 2263 567
Size: 1696 2200
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 796 796
Size: 3896
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 562 712 1072 1951 2443 2741 562
Size: 150 360 879 492 298 1717
Fragments arranged by size:
1717 879 492 360 298 150
BsaI GGTCTCn'nnnn_
Cuts at: 3600 3600
Size: 3896
BsaAI yAC'GTr
Cuts at: 141 618 1249 141
Size: 477 631 2788
Fragments arranged by size:
2788 631 477
BsaBI GATnn'nnATC
Cuts at: 6 1410 3447 6
Size: 1404 2037 455
Fragments arranged by size:
2037 1404 455
BsaHI Gr'CG_yC
Cuts at: 235 432 759 1327 1575 2277 3773 235
Size: 197 327 568 248 702 1496 358
Fragments arranged by size:
1496 702 568 358 327 248 197
BsaWI w'CCGG_w
Cuts at: 1085 1606 2284 2558 2705 3116 1085
Size: 521 678 274 147 411 1865
Fragments arranged by size:
1865 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 306 345 1321 2079 2656 3152 306
Size: 39 976 758 577 496 1050
Fragments arranged by size:
1050 976 758 577 496 39
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 307 424 307
Size: 117 3779
BsiEI CG_ry'CG
Cuts at: 104 1438 1484 2692 104
Size: 1334 46 1208 1308
Fragments arranged by size:
1334 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 140 224 615 704 812 910 1688 1878 2670
Size: 84 391 89 108 98 778 190 792
Cuts at: 2670 140
Size: 1366
Fragments arranged by size:
1366 792 778 391 190 108 98 89
84
BsmBI CGTCTCn'nnnn_
Cuts at: 555 766 555
Size: 211 3685
Bsp1286I G_dGCh'C
Cuts at: 140 224 615 704 812 910 1521 1614 1688
Size: 84 391 89 108 98 611 93 74
Cuts at: 1688 1878 1940 2670 140
Size: 190 62 730 1366
Fragments arranged by size:
1366 730 611 391 190 108 98 93
89 84 74 62
BspEI T'CCGG_A
Cuts at: 3116 3116
Size: 3896
BspHI T'CATG_A
Cuts at: 3072 3072
Size: 3896
BspMI ACCTGCnnnn'nnnn_
Cuts at: 946 1160 1462 1843 946
Size: 214 302 381 2999
Fragments arranged by size:
2999 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2188 2242 3726 3891 50
Size: 2138 54 1484 165 55
Fragments arranged by size:
2138 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1808 1808
Size: 3896
BsrFI r'CCGG_y
Cuts at: 151 244 1894 2075 2284 151
Size: 93 1650 181 209 1763
Fragments arranged by size:
1763 1650 209 181 93
BssSI C'ACGA_G
Cuts at: 283 2167 2525 283
Size: 1884 358 1654
Fragments arranged by size:
1884 1654 358
BstAPI GCAn_nnn'nTGC
Cuts at: 411 610 642 1273 411
Size: 199 32 631 3034
Fragments arranged by size:
3034 631 199 32
BstBI TT'CG_AA
Cuts at: 2257 2257
Size: 3896
BstXI CCAn_nnnn'nTGG
Cuts at: 874 874
Size: 3896
BstYI r'GATC_y
Cuts at: 1 921 1388 1411 1746 1992 3004 3090 3102
Size: 920 467 23 335 246 1012 86 12
Cuts at: 3102 3448 1
Size: 346 449
Fragments arranged by size:
1012 920 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 146 167 239 380 440 485 510 146
Size: 21 72 141 60 45 25 3532
Fragments arranged by size:
3532 141 72 60 45 25 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 352 1846 2027 2190 352
Size: 1494 181 163 2058
Fragments arranged by size:
2058 1494 181 163
BtsI GCAGTG_nn'
Cuts at: 412 433 463 1139 412
Size: 21 30 676 3169
Fragments arranged by size:
3169 676 30 21
DraI TTT'AAA
Cuts at: 895 895
Size: 3896
DraIII CAC_nnn'GTG
Cuts at: 610 621 610
Size: 11 3885
DrdI GACnn_nn'nnGTC
Cuts at: 1602 2460 1602
Size: 858 3038
EaeI y'GGCC_r
Cuts at: 143 149 242 263 557 623 1481 1655 2046
Size: 6 93 21 294 66 858 174 391
Cuts at: 2046 2073 3418 143
Size: 27 1345 621
Fragments arranged by size:
1345 858 621 391 294 174 93 66
27 21 6
EagI C'GGCC_G
Cuts at: 1481 1481
Size: 3896
EarI CTCTTCn'nnn_
Cuts at: 769 1919 2129 769
Size: 1150 210 2536
Fragments arranged by size:
2536 1150 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2104 2414 2560 3706 2104
Size: 310 146 1146 2294
Fragments arranged by size:
2294 1146 310 146
EcoICRI GAG'CTC
Cuts at: 908 908
Size: 3896
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 382 796 1721 2153 2900 3168 382
Size: 414 925 432 747 268 1110
Fragments arranged by size:
1110 925 747 432 414 268
EcoO109I rG'GnC_Cy
Cuts at: 349 1035 349
Size: 686 3210
EcoRI G'AATT_C
Cuts at: 900 900
Size: 3896
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1915 1915
Size: 3896
FseI GG_CCGG'CC
Cuts at: 248 248
Size: 3896
FspI TGC'GCA
Cuts at: 646 1677 646
Size: 1031 2865
HaeII r_GCGC'y
Cuts at: 238 435 643 1330 1578 2600 3579 238
Size: 197 208 687 248 1022 979 555
Fragments arranged by size:
1022 979 687 555 248 208 197
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1738 1768 1770 1800 1738
Size: 30 2 30 3834
Fragments arranged by size:
3834 30 30 2
HincII GTy'rAC
Cuts at: 935 935
Size: 3896
KasI G'GCGC_C
Cuts at: 234 431 1326 1574 234
Size: 197 895 248 2556
Fragments arranged by size:
2556 895 248 197
KpnI G_GTAC'C
Cuts at: 916 916
Size: 3896
MluI A'CGCG_T
Cuts at: 886 3329 886
Size: 2443 1453
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2567 2751 2567
Size: 184 3712
MscI TGG'CCA
Cuts at: 265 559 1657 265
Size: 294 1098 2504
Fragments arranged by size:
2504 1098 294
MslI CAynn'nnrTG
Cuts at: 1885 2012 1885
Size: 127 3769
MspA1I CmG'CkG
Cuts at: 442 512 628 1007 1321 1681 2694 2939 442
Size: 70 116 379 314 360 1013 245 1399
Fragments arranged by size:
1399 1013 379 360 314 245 116 70
NaeI GCC'GGC
Cuts at: 246 2077 246
Size: 1831 2065
NarI GG'CG_CC
Cuts at: 235 432 1327 1575 235
Size: 197 895 248 2556
Fragments arranged by size:
2556 895 248 197
NcoI C'CATG_G
Cuts at: 380 380
Size: 3896
NgoMIV G'CCGG_C
Cuts at: 244 2075 244
Size: 1831 2065
NspI r_CATG'y
Cuts at: 949 1449 2356 949
Size: 500 907 2489
Fragments arranged by size:
2489 907 500
PasI CC'CwG_GG
Cuts at: 588 588
Size: 3896
PciI A'CATG_T
Cuts at: 2352 2352
Size: 3896
PmeI GTTT'AAAC
Cuts at: 895 895
Size: 3896
PmlI CAC'GTG
Cuts at: 141 618 141
Size: 477 3419
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3035 3067 3035
Size: 32 3864
PpuMI rG'GwC_Cy
Cuts at: 349 349
Size: 3896
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 280 312 280
Size: 32 3864
PstI C_TGCA'G
Cuts at: 943 1628 943
Size: 685 3211
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3896
PvuII CAG'CTG
Cuts at: 1321 1681 1321
Size: 360 3536
RsrII CG'GwC_CG
Cuts at: 2091 2091
Size: 3896
SacI G_AGCT'C
Cuts at: 910 910
Size: 3896
SacII CC_GC'GG
Cuts at: 443 513 443
Size: 70 3826
SalI G'TCGA_C
Cuts at: 933 933
Size: 3896
SanDI GG'GwC_CC
Cuts at: 349 349
Size: 3896
SapI GCTCTTCn'nnn_
Cuts at: 1919 2129 1919
Size: 210 3686
SbfI CC_TGCA'GG
Cuts at: 943 943
Size: 3896
ScaI AGT'ACT
Cuts at: 3413 3413
Size: 3896
SfcI C'TryA_G
Cuts at: 33 939 1624 2617 2808 33
Size: 906 685 993 191 1121
Fragments arranged by size:
1121 993 906 685 191
SfoI GGC'GCC
Cuts at: 236 433 1328 1576 236
Size: 197 895 248 2556
Fragments arranged by size:
2556 895 248 197
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3896
SmaI CCC'GGG
Cuts at: 918 918
Size: 3896
SmlI C'TyrA_G
Cuts at: 541 727 1051 1966 2458 2720 541
Size: 186 324 915 492 262 1717
Fragments arranged by size:
1717 915 492 324 262 186
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3896
SphI G_CATG'C
Cuts at: 949 1449 949
Size: 500 3396
StuI AGG'CCT
Cuts at: 130 130
Size: 3896
StyI C'CwwG_G
Cuts at: 380 794 1030 1384 380
Size: 414 236 354 2892
Fragments arranged by size:
2892 414 354 236
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 598 664 872 1479 1612 2071 2280 598
Size: 66 208 607 133 459 209 2214
Fragments arranged by size:
2214 607 459 209 208 133 66
TatI w'GTAC_w
Cuts at: 3411 3411
Size: 3896
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 775 807 873 905 3413 3445 775
Size: 32 66 32 2508 32 1226
Fragments arranged by size:
2508 1226 66 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1693 1693
Size: 3896
XbaI T'CTAG_A
Cuts at: 927 927
Size: 3896
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 658 1391 658
Size: 733 3163
XmaI C'CCGG_G
Cuts at: 916 916
Size: 3896
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FseI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PmeI PmlI PpiI PpuMI
PsrI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BmtI
Bpu10I BsaXI BseRI BsiWI BsmI BsrGI BssHII BstEII
BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PflMI PfoI PshAI PsiI PspOMI PspXI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI