Restriction map for
pF1KE0134
(Circular) (Six-base) MAPSORT of: pF1KE0134.seq Check: 6889 from: 1 to: 3755
pF1KE0134 3755 bp
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AccI GT'mk_AC
Cuts at: 793 793
Size: 3755
Acc65I G'GTAC_C
Cuts at: 771 771
Size: 3755
AclI AA'CG_TT
Cuts at: 3595 3595
Size: 3755
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 314 1580 2012 2759 3027 314
Size: 1266 432 747 268 1042
Fragments arranged by size:
1266 1042 747 432 268
AflIII A'CryG_T
Cuts at: 1107 2211 3188 1107
Size: 1104 977 1674
Fragments arranged by size:
1674 1104 977
AgeI A'CCGG_T
Cuts at: 134 2143 134
Size: 2009 1746
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 547 547
Size: 3755
AlwNI CAG_nnn'CTG
Cuts at: 1060 2627 1060
Size: 1567 2188
ApaI G_GGCC'C
Cuts at: 741 741
Size: 3755
ApaLI G'TGCA_C
Cuts at: 2525 2525
Size: 3755
ApoI r'AATT_y
Cuts at: 314 646 759 3108 3364 314
Size: 332 113 2349 256 705
Fragments arranged by size:
2349 705 332 256 113
AseI AT'TA_AT
Cuts at: 20 20
Size: 3755
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3755
AvaI C'yCGr_G
Cuts at: 775 775
Size: 3755
BamHI G'GATC_C
Cuts at: 780 780
Size: 3755
BanI G'GyrC_C
Cuts at: 457 771 1185 1433 1468 457
Size: 314 414 248 35 2744
Fragments arranged by size:
2744 414 314 248 35
BanII G_rGCy'C
Cuts at: 741 769 1799 741
Size: 28 1030 2697
Fragments arranged by size:
2697 1030 28
BbeI G_GCGC'C
Cuts at: 1189 1437 1189
Size: 248 3507
BbsI GAAGACnn'nnnn_
Cuts at: 659 659
Size: 3755
BciVI GTATCCnnnnn_n'
Cuts at: 1653 2414 3755 1653
Size: 761 1341 1653
Fragments arranged by size:
1653 1341 761
BclI T'GATC_A
Cuts at: 1275 1275
Size: 3755
BglI GCCn_nnn'nGGC
Cuts at: 304 304
Size: 3755
BglII A'GATC_T
Cuts at: 1270 3307 1270
Size: 2037 1718
BlpI GC'TnA_GC
Cuts at: 867 867
Size: 3755
Bme1580I G_kGCm'C
Cuts at: 481 741 1380 1473 2529 481
Size: 260 639 93 1056 1707
Fragments arranged by size:
1707 1056 639 260 93
BmgBI CAC'GTC
Cuts at: 393 393
Size: 3755
BmrI ACTGGGnnnn_n'
Cuts at: 704 967 1048 1383 3527 704
Size: 263 81 335 2144 932
Fragments arranged by size:
2144 932 335 263 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2122 2122
Size: 3755
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 322 322
Size: 3755
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 931 1810 2302 2600 931
Size: 879 492 298 2086
Fragments arranged by size:
2086 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3459 3459
Size: 3755
BsaAI yAC'GTr
Cuts at: 1108 1108
Size: 3755
BsaBI GATnn'nnATC
Cuts at: 6 604 1269 3306 6
Size: 598 665 2037 455
Fragments arranged by size:
2037 665 598 455
BsaHI Gr'CG_yC
Cuts at: 1186 1434 2136 3632 1186
Size: 248 702 1496 1309
Fragments arranged by size:
1496 1309 702 248
BsaWI w'CCGG_w
Cuts at: 134 944 1465 2143 2417 2564 2975 134
Size: 810 521 678 274 147 411 914
Fragments arranged by size:
914 810 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 424 1180 1938 2515 3011 424
Size: 756 758 577 496 1168
Fragments arranged by size:
1168 758 756 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 727 727
Size: 3755
BsiEI CG_ry'CG
Cuts at: 104 1297 1343 2551 104
Size: 1193 46 1208 1308
Fragments arranged by size:
1308 1208 1193 46
BsiHKAI G_wGCw'C
Cuts at: 769 1547 1737 2529 769
Size: 778 190 792 1995
Fragments arranged by size:
1995 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 481 741 769 1380 1473 1547 1737 1799 2529
Size: 260 28 611 93 74 190 62 730
Cuts at: 2529 481
Size: 1707
Fragments arranged by size:
1707 730 611 260 190 93 74 62
28
BspEI T'CCGG_A
Cuts at: 2975 2975
Size: 3755
BspHI T'CATG_A
Cuts at: 2931 2931
Size: 3755
BspMI ACCTGCnnnn'nnnn_
Cuts at: 805 1019 1321 1702 805
Size: 214 302 381 2858
Fragments arranged by size:
2858 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2047 2101 3585 3750 50
Size: 1997 54 1484 165 55
Fragments arranged by size:
1997 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1667 1667
Size: 3755
BsrFI r'CCGG_y
Cuts at: 134 1753 1934 2143 134
Size: 1619 181 209 1746
Fragments arranged by size:
1746 1619 209 181
BssSI C'ACGA_G
Cuts at: 2026 2384 2026
Size: 358 3397
BstAPI GCAn_nnn'nTGC
Cuts at: 1132 1132
Size: 3755
BstBI TT'CG_AA
Cuts at: 2116 2116
Size: 3755
BstYI r'GATC_y
Cuts at: 1 599 780 1247 1270 1605 1851 2863 2949
Size: 598 181 467 23 335 246 1012 86
Cuts at: 2949 2961 3307 1
Size: 12 346 449
Fragments arranged by size:
1012 598 467 449 346 335 246 181
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3755
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1705 1886 2049 1705
Size: 181 163 3411
Fragments arranged by size:
3411 181 163
BtsI GCAGTG_nn'
Cuts at: 998 998
Size: 3755
DraI TTT'AAA
Cuts at: 754 754
Size: 3755
DraIII CAC_nnn'GTG
Cuts at: 264 264
Size: 3755
DrdI GACnn_nn'nnGTC
Cuts at: 1461 2319 1461
Size: 858 2897
EaeI y'GGCC_r
Cuts at: 255 485 501 588 1340 1514 1905 1932 3277
Size: 230 16 87 752 174 391 27 1345
Cuts at: 3277 255
Size: 733
Fragments arranged by size:
1345 752 733 391 230 174 87 27
16
EagI C'GGCC_G
Cuts at: 1340 1340
Size: 3755
EarI CTCTTCn'nnn_
Cuts at: 1778 1988 1778
Size: 210 3545
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 234 1963 2273 2419 3565 234
Size: 1729 310 146 1146 424
Fragments arranged by size:
1729 1146 424 310 146
EcoICRI GAG'CTC
Cuts at: 767 767
Size: 3755
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 314 322 1580 2012 2759 3027 314
Size: 8 1258 432 747 268 1042
Fragments arranged by size:
1258 1042 747 432 268 8
EcoO109I rG'GnC_Cy
Cuts at: 558 738 894 558
Size: 180 156 3419
Fragments arranged by size:
3419 180 156
EcoRI G'AATT_C
Cuts at: 759 759
Size: 3755
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 186 639 1774 186
Size: 453 1135 2167
Fragments arranged by size:
2167 1135 453
FspI TGC'GCA
Cuts at: 565 1536 565
Size: 971 2784
HaeII r_GCGC'y
Cuts at: 1189 1437 2459 3438 1189
Size: 248 1022 979 1506
Fragments arranged by size:
1506 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 271 303 1597 1627 1629 1659 271
Size: 32 1294 30 2 30 2367
Fragments arranged by size:
2367 1294 32 30 30 2
HincII GTy'rAC
Cuts at: 794 794
Size: 3755
KasI G'GCGC_C
Cuts at: 1185 1433 1185
Size: 248 3507
KpnI G_GTAC'C
Cuts at: 775 775
Size: 3755
MluI A'CGCG_T
Cuts at: 3188 3188
Size: 3755
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2426 2610 2426
Size: 184 3571
MscI TGG'CCA
Cuts at: 257 487 503 1516 257
Size: 230 16 1013 2496
Fragments arranged by size:
2496 1013 230 16
MslI CAynn'nnrTG
Cuts at: 1744 1871 1744
Size: 127 3628
MspA1I CmG'CkG
Cuts at: 285 866 1180 1540 2553 2798 285
Size: 581 314 360 1013 245 1242
Fragments arranged by size:
1242 1013 581 360 314 245
NaeI GCC'GGC
Cuts at: 1936 1936
Size: 3755
NarI GG'CG_CC
Cuts at: 1186 1434 1186
Size: 248 3507
NcoI C'CATG_G
Cuts at: 107 107
Size: 3755
NgoMIV G'CCGG_C
Cuts at: 1934 1934
Size: 3755
NspI r_CATG'y
Cuts at: 453 550 808 1308 2215 453
Size: 97 258 500 907 1993
Fragments arranged by size:
1993 907 500 258 97
PasI CC'CwG_GG
Cuts at: 474 474
Size: 3755
PciI A'CATG_T
Cuts at: 2211 2211
Size: 3755
PmeI GTTT'AAAC
Cuts at: 754 754
Size: 3755
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2894 2926 2894
Size: 32 3723
PspOMI G'GGCC_C
Cuts at: 737 737
Size: 3755
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 139 171 139
Size: 32 3723
PstI C_TGCA'G
Cuts at: 536 802 1487 536
Size: 266 685 2804
Fragments arranged by size:
2804 685 266
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3755
PvuII CAG'CTG
Cuts at: 285 1180 1540 285
Size: 895 360 2500
Fragments arranged by size:
2500 895 360
RsrII CG'GwC_CG
Cuts at: 1950 1950
Size: 3755
SacI G_AGCT'C
Cuts at: 769 769
Size: 3755
SalI G'TCGA_C
Cuts at: 792 792
Size: 3755
SapI GCTCTTCn'nnn_
Cuts at: 1778 1988 1778
Size: 210 3545
SbfI CC_TGCA'GG
Cuts at: 802 802
Size: 3755
ScaI AGT'ACT
Cuts at: 3272 3272
Size: 3755
SfcI C'TryA_G
Cuts at: 33 532 798 1483 2476 2667 33
Size: 499 266 685 993 191 1121
Fragments arranged by size:
1121 993 685 499 266 191
SfoI GGC'GCC
Cuts at: 1187 1435 1187
Size: 248 3507
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3755
SmaI CCC'GGG
Cuts at: 777 777
Size: 3755
SmlI C'TyrA_G
Cuts at: 910 1825 2317 2579 910
Size: 915 492 262 2086
Fragments arranged by size:
2086 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3755
SphI G_CATG'C
Cuts at: 453 808 1308 453
Size: 355 500 2900
Fragments arranged by size:
2900 500 355
StyI C'CwwG_G
Cuts at: 107 889 1243 107
Size: 782 354 2619
Fragments arranged by size:
2619 782 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1338 1471 1930 2139 1338
Size: 133 459 209 2954
Fragments arranged by size:
2954 459 209 133
TatI w'GTAC_w
Cuts at: 329 701 3270 329
Size: 372 2569 814
Fragments arranged by size:
2569 814 372
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3272 3304 3272
Size: 32 3723
Tth111I GACn'n_nGTC
Cuts at: 1552 1552
Size: 3755
XbaI T'CTAG_A
Cuts at: 786 786
Size: 3755
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 330 1250 330
Size: 920 2835
XmaI C'CCGG_G
Cuts at: 775 775
Size: 3755
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PmeI PpiI PspOMI PsrI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI AscI
AvrII BaeI BbvCI BcgI BfrBI BmtI Bpu10I BsaXI
BseRI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PflMI PfoI PmlI PpuMI PshAI PsiI PspXI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI