Restriction map for
pF1KE0135
(Circular) (Six-base) MAPSORT of: pF1KE0135.seq Check: 9618 from: 1 to: 4130
pF1KE0135 4130 bp
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AccI GT'mk_AC
Cuts at: 1168 1168
Size: 4130
Acc65I G'GTAC_C
Cuts at: 1146 1146
Size: 4130
AclI AA'CG_TT
Cuts at: 3970 3970
Size: 4130
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 699 804 1955 2387 3134 3402 699
Size: 105 1151 432 747 268 1427
Fragments arranged by size:
1427 1151 747 432 268 105
AflIII A'CryG_T
Cuts at: 1482 2586 3563 1482
Size: 1104 977 2049
Fragments arranged by size:
2049 1104 977
AgeI A'CCGG_T
Cuts at: 2518 2518
Size: 4130
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 790 1042 790
Size: 252 3878
AlwNI CAG_nnn'CTG
Cuts at: 722 786 1076 1435 3002 722
Size: 64 290 359 1567 1850
Fragments arranged by size:
1850 1567 359 290 64
ApaLI G'TGCA_C
Cuts at: 2900 2900
Size: 4130
ApoI r'AATT_y
Cuts at: 1134 3483 3739 1134
Size: 2349 256 1525
Fragments arranged by size:
2349 1525 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4130
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4130
AvaI C'yCGr_G
Cuts at: 799 1150 799
Size: 351 3779
BamHI G'GATC_C
Cuts at: 1155 1155
Size: 4130
BanI G'GyrC_C
Cuts at: 124 737 1146 1560 1808 1843 124
Size: 613 409 414 248 35 2411
Fragments arranged by size:
2411 613 414 409 248 35
BanII G_rGCy'C
Cuts at: 287 1144 2174 287
Size: 857 1030 2243
Fragments arranged by size:
2243 1030 857
BbeI G_GCGC'C
Cuts at: 128 1564 1812 128
Size: 1436 248 2446
Fragments arranged by size:
2446 1436 248
BbvCI CC'TCA_GC
Cuts at: 829 1030 829
Size: 201 3929
BciVI GTATCCnnnnn_n'
Cuts at: 2028 2789 4130 2028
Size: 761 1341 2028
Fragments arranged by size:
2028 1341 761
BclI T'GATC_A
Cuts at: 875 1650 875
Size: 775 3355
BglII A'GATC_T
Cuts at: 455 1645 3682 455
Size: 1190 2037 903
Fragments arranged by size:
2037 1190 903
BlpI GC'TnA_GC
Cuts at: 1242 1242
Size: 4130
Bme1580I G_kGCm'C
Cuts at: 740 1755 1848 2904 740
Size: 1015 93 1056 1966
Fragments arranged by size:
1966 1056 1015 93
BmrI ACTGGGnnnn_n'
Cuts at: 583 732 1342 1423 1758 3902 583
Size: 149 610 81 335 2144 811
Fragments arranged by size:
2144 811 610 335 149 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2497 2497
Size: 4130
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 430 444 499 430
Size: 14 55 4061
Fragments arranged by size:
4061 55 14
Bpu10I CC'TnA_GC
Cuts at: 829 1030 829
Size: 201 3929
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 271 1306 2185 2677 2975 271
Size: 1035 879 492 298 1426
Fragments arranged by size:
1426 1035 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 310 611 3834 310
Size: 301 3223 606
Fragments arranged by size:
3223 606 301
BsaAI yAC'GTr
Cuts at: 1483 1483
Size: 4130
BsaBI GATnn'nnATC
Cuts at: 6 706 1644 3681 6
Size: 700 938 2037 455
Fragments arranged by size:
2037 938 700 455
BsaHI Gr'CG_yC
Cuts at: 125 986 1561 1809 2511 4007 125
Size: 861 575 248 702 1496 248
Fragments arranged by size:
1496 861 702 575 248 248
BsaWI w'CCGG_w
Cuts at: 1319 1840 2518 2792 2939 3350 1319
Size: 521 678 274 147 411 2099
Fragments arranged by size:
2099 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 232 496 517 526 544 547 640 643 646
Size: 264 21 9 18 3 93 3 3
Cuts at: 646 712 1047 232
Size: 66 335 3315
Fragments arranged by size:
3315 335 264 93 66 21 18 9
3 3 3
BseYI C'CCAG_C
Cuts at: 1076 1555 2313 2890 3386 1076
Size: 479 758 577 496 1820
Fragments arranged by size:
1820 758 577 496 479
BsiEI CG_ry'CG
Cuts at: 104 144 1672 1718 2926 104
Size: 40 1528 46 1208 1308
Fragments arranged by size:
1528 1308 1208 46 40
BsiHKAI G_wGCw'C
Cuts at: 287 1144 1922 2112 2904 287
Size: 857 778 190 792 1513
Fragments arranged by size:
1513 857 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 287 740 1144 1755 1848 1922 2112 2174 2904
Size: 453 404 611 93 74 190 62 730
Cuts at: 2904 287
Size: 1513
Fragments arranged by size:
1513 730 611 453 404 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3350 3350
Size: 4130
BspHI T'CATG_A
Cuts at: 227 960 3306 227
Size: 733 2346 1051
Fragments arranged by size:
2346 1051 733
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1180 1394 1696 2077 1180
Size: 214 302 381 3233
Fragments arranged by size:
3233 381 302 214
BsrBI CCG'CTC
Cuts at: 50 357 2422 2476 3960 4125 50
Size: 307 2065 54 1484 165 55
Fragments arranged by size:
2065 1484 307 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2042 2042
Size: 4130
BsrFI r'CCGG_y
Cuts at: 139 740 2128 2309 2518 139
Size: 601 1388 181 209 1751
Fragments arranged by size:
1751 1388 601 209 181
BsrGI T'GTAC_A
Cuts at: 857 857
Size: 4130
BssSI C'ACGA_G
Cuts at: 307 2401 2759 307
Size: 2094 358 1678
Fragments arranged by size:
2094 1678 358
BstAPI GCAn_nnn'nTGC
Cuts at: 786 1507 786
Size: 721 3409
BstBI TT'CG_AA
Cuts at: 2491 2491
Size: 4130
BstXI CCAn_nnnn'nTGG
Cuts at: 300 300
Size: 4130
BstYI r'GATC_y
Cuts at: 1 455 803 1155 1622 1645 1980 2226 3238
Size: 454 348 352 467 23 335 246 1012
Cuts at: 3238 3324 3336 3682 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 454 449 352 348 346 335
246 86 23 12
BtgI C'CryG_G
Cuts at: 107 299 107
Size: 192 3938
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 195 2080 2261 2424 195
Size: 1885 181 163 1901
Fragments arranged by size:
1901 1885 181 163
BtsI GCAGTG_nn'
Cuts at: 1373 1373
Size: 4130
DraI TTT'AAA
Cuts at: 1129 1129
Size: 4130
DrdI GACnn_nn'nnGTC
Cuts at: 910 1836 2694 910
Size: 926 858 2346
Fragments arranged by size:
2346 926 858
EaeI y'GGCC_r
Cuts at: 141 371 518 1715 1889 2280 2307 3652 141
Size: 230 147 1197 174 391 27 1345 619
Fragments arranged by size:
1345 1197 619 391 230 174 147 27
EagI C'GGCC_G
Cuts at: 141 1715 141
Size: 1574 2556
EarI CTCTTCn'nnn_
Cuts at: 485 503 542 559 644 925 949 2153 2363
Size: 18 39 17 85 281 24 1204 210
Cuts at: 2363 485
Size: 2252
Fragments arranged by size:
2252 1204 281 210 85 39 24 18
17
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 759 2338 2648 2794 3940 759
Size: 1579 310 146 1146 949
Fragments arranged by size:
1579 1146 949 310 146
EcoICRI GAG'CTC
Cuts at: 285 1142 285
Size: 857 3273
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 430 444 499 699 804 1955 2387 3134 3402
Size: 14 55 200 105 1151 432 747 268
Cuts at: 3402 430
Size: 1158
Fragments arranged by size:
1158 1151 747 432 268 200 105 55
14
EcoNI CCTnn'n_nnAGG
Cuts at: 481 790 481
Size: 309 3821
EcoO109I rG'GnC_Cy
Cuts at: 1269 1269
Size: 4130
EcoRI G'AATT_C
Cuts at: 1134 1134
Size: 4130
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 888 1077 2149 888
Size: 189 1072 2869
Fragments arranged by size:
2869 1072 189
FseI GG_CCGG'CC
Cuts at: 143 143
Size: 4130
FspI TGC'GCA
Cuts at: 260 1911 260
Size: 1651 2479
HaeII r_GCGC'y
Cuts at: 128 799 1564 1812 2834 3813 128
Size: 671 765 248 1022 979 445
Fragments arranged by size:
1022 979 765 671 445 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 274 306 817 849 867 899 1972 2002 2004
Size: 32 511 32 18 32 1073 30 2
Cuts at: 2004 2034 274
Size: 30 2370
Fragments arranged by size:
2370 1073 511 32 32 32 30 30
18 2
HincII GTy'rAC
Cuts at: 594 1169 594
Size: 575 3555
HindIII A'AGCT_T
Cuts at: 415 600 415
Size: 185 3945
KasI G'GCGC_C
Cuts at: 124 1560 1808 124
Size: 1436 248 2446
Fragments arranged by size:
2446 1436 248
KpnI G_GTAC'C
Cuts at: 1150 1150
Size: 4130
MluI A'CGCG_T
Cuts at: 3563 3563
Size: 4130
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 260 2801 2985 260
Size: 2541 184 1405
Fragments arranged by size:
2541 1405 184
MscI TGG'CCA
Cuts at: 373 1891 373
Size: 1518 2612
MslI CAynn'nnrTG
Cuts at: 965 2119 2246 965
Size: 1154 127 2849
Fragments arranged by size:
2849 1154 127
MspA1I CmG'CkG
Cuts at: 369 1076 1241 1555 1915 2928 3173 369
Size: 707 165 314 360 1013 245 1326
Fragments arranged by size:
1326 1013 707 360 314 245 165
NaeI GCC'GGC
Cuts at: 141 2311 141
Size: 2170 1960
NarI GG'CG_CC
Cuts at: 125 1561 1809 125
Size: 1436 248 2446
Fragments arranged by size:
2446 1436 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4130
NgoMIV G'CCGG_C
Cuts at: 139 2309 139
Size: 2170 1960
NspI r_CATG'y
Cuts at: 1183 1683 2590 1183
Size: 500 907 2723
Fragments arranged by size:
2723 907 500
PciI A'CATG_T
Cuts at: 2586 2586
Size: 4130
PflMI CCAn_nnn'nTGG
Cuts at: 1076 1076
Size: 4130
PmeI GTTT'AAAC
Cuts at: 1129 1129
Size: 4130
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3269 3301 3269
Size: 32 4098
PstI C_TGCA'G
Cuts at: 401 791 1177 1862 401
Size: 390 386 685 2669
Fragments arranged by size:
2669 685 390 386
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4130
PvuII CAG'CTG
Cuts at: 369 1555 1915 369
Size: 1186 360 2584
Fragments arranged by size:
2584 1186 360
RsrII CG'GwC_CG
Cuts at: 2325 2325
Size: 4130
SacI G_AGCT'C
Cuts at: 287 1144 287
Size: 857 3273
SalI G'TCGA_C
Cuts at: 1167 1167
Size: 4130
SapI GCTCTTCn'nnn_
Cuts at: 644 2153 2363 644
Size: 1509 210 2411
Fragments arranged by size:
2411 1509 210
SbfI CC_TGCA'GG
Cuts at: 1177 1177
Size: 4130
ScaI AGT'ACT
Cuts at: 3647 3647
Size: 4130
SfcI C'TryA_G
Cuts at: 33 397 787 1173 1858 2851 3042 33
Size: 364 390 386 685 993 191 1121
Fragments arranged by size:
1121 993 685 390 386 364 191
SfoI GGC'GCC
Cuts at: 126 1562 1810 126
Size: 1436 248 2446
Fragments arranged by size:
2446 1436 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4130
SgrAI Cr'CCGG_yG
Cuts at: 740 740
Size: 4130
SmaI CCC'GGG
Cuts at: 1152 1152
Size: 4130
SmlI C'TyrA_G
Cuts at: 286 799 1285 2200 2692 2954 286
Size: 513 486 915 492 262 1462
Fragments arranged by size:
1462 915 513 492 486 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4130
SphI G_CATG'C
Cuts at: 1183 1683 1183
Size: 500 3630
StyI C'CwwG_G
Cuts at: 107 389 917 1264 1618 107
Size: 282 528 347 354 2619
Fragments arranged by size:
2619 528 354 347 282
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1713 1846 2305 2514 1713
Size: 133 459 209 3329
Fragments arranged by size:
3329 459 209 133
TatI w'GTAC_w
Cuts at: 857 1112 3645 857
Size: 255 2533 1342
Fragments arranged by size:
2533 1342 255
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3647 3679 3647
Size: 32 4098
Tth111I GACn'n_nGTC
Cuts at: 1927 1927
Size: 4130
XbaI T'CTAG_A
Cuts at: 1161 1161
Size: 4130
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1625 1625
Size: 4130
XhoI C'TCGA_G
Cuts at: 799 799
Size: 4130
XmaI C'CCGG_G
Cuts at: 1150 1150
Size: 4130
XmnI GAAnn'nnTTC
Cuts at: 817 817
Size: 4130
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbvCI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FseI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PflMI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbsI BcgI BfrBI BglI BmgBI
BmtI BsaXI BsgI BsiWI BsmI BsmBI BssHII BstEII
BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FspAI HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SnaBI SrfI SspI StuI
SwaI ZraI