Restriction map for
pF1KE0137
(Circular) (Six-base) MAPSORT of: pF1KE0137.seq Check: 4852 from: 1 to: 3629
pF1KE0137 3629 bp
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AccI GT'mk_AC
Cuts at: 667 667
Size: 3629
Acc65I G'GTAC_C
Cuts at: 645 645
Size: 3629
AclI AA'CG_TT
Cuts at: 3469 3469
Size: 3629
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 206 286 1454 1886 2633 2901 206
Size: 80 1168 432 747 268 934
Fragments arranged by size:
1168 934 747 432 268 80
AflIII A'CryG_T
Cuts at: 489 981 2085 3062 489
Size: 492 1104 977 1056
Fragments arranged by size:
1104 1056 977 492
AgeI A'CCGG_T
Cuts at: 2017 2017
Size: 3629
AlwNI CAG_nnn'CTG
Cuts at: 934 2501 934
Size: 1567 2062
ApaI G_GGCC'C
Cuts at: 285 285
Size: 3629
ApaLI G'TGCA_C
Cuts at: 2399 2399
Size: 3629
ApoI r'AATT_y
Cuts at: 633 2982 3238 633
Size: 2349 256 1024
Fragments arranged by size:
2349 1024 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3629
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3629
AvaI C'yCGr_G
Cuts at: 649 649
Size: 3629
BamHI G'GATC_C
Cuts at: 654 654
Size: 3629
BanI G'GyrC_C
Cuts at: 244 645 1059 1307 1342 244
Size: 401 414 248 35 2531
Fragments arranged by size:
2531 414 401 248 35
BanII G_rGCy'C
Cuts at: 169 285 643 1673 169
Size: 116 358 1030 2125
Fragments arranged by size:
2125 1030 358 116
BbeI G_GCGC'C
Cuts at: 1063 1311 1063
Size: 248 3381
BciVI GTATCCnnnnn_n'
Cuts at: 1527 2288 3629 1527
Size: 761 1341 1527
Fragments arranged by size:
1527 1341 761
BclI T'GATC_A
Cuts at: 1149 1149
Size: 3629
BglII A'GATC_T
Cuts at: 1144 3181 1144
Size: 2037 1592
BlpI GC'TnA_GC
Cuts at: 741 741
Size: 3629
Bme1580I G_kGCm'C
Cuts at: 285 1254 1347 2403 285
Size: 969 93 1056 1511
Fragments arranged by size:
1511 1056 969 93
BmrI ACTGGGnnnn_n'
Cuts at: 470 841 922 1257 3401 470
Size: 371 81 335 2144 698
Fragments arranged by size:
2144 698 371 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1996 1996
Size: 3629
Bpu10I CC'TnA_GC
Cuts at: 170 176 170
Size: 6 3623
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 805 1684 2176 2474 805
Size: 879 492 298 1960
Fragments arranged by size:
1960 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3333 3333
Size: 3629
BsaAI yAC'GTr
Cuts at: 982 982
Size: 3629
BsaBI GATnn'nnATC
Cuts at: 6 1143 3180 6
Size: 1137 2037 455
Fragments arranged by size:
2037 1137 455
BsaHI Gr'CG_yC
Cuts at: 1060 1308 2010 3506 1060
Size: 248 702 1496 1183
Fragments arranged by size:
1496 1183 702 248
BsaWI w'CCGG_w
Cuts at: 818 1339 2017 2291 2438 2849 818
Size: 521 678 274 147 411 1598
Fragments arranged by size:
1598 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 541 541
Size: 3629
BseYI C'CCAG_C
Cuts at: 1054 1812 2389 2885 1054
Size: 758 577 496 1798
Fragments arranged by size:
1798 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1171 1217 2425 104
Size: 1067 46 1208 1308
Fragments arranged by size:
1308 1208 1067 46
BsiHKAI G_wGCw'C
Cuts at: 643 1421 1611 2403 643
Size: 778 190 792 1869
Fragments arranged by size:
1869 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 169 285 643 1254 1347 1421 1611 1673 2403
Size: 116 358 611 93 74 190 62 730
Cuts at: 2403 169
Size: 1395
Fragments arranged by size:
1395 730 611 358 190 116 93 74
62
BspEI T'CCGG_A
Cuts at: 2849 2849
Size: 3629
BspHI T'CATG_A
Cuts at: 602 2805 602
Size: 2203 1426
BspMI ACCTGCnnnn'nnnn_
Cuts at: 679 893 1195 1576 679
Size: 214 302 381 2732
Fragments arranged by size:
2732 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1921 1975 3459 3624 50
Size: 1871 54 1484 165 55
Fragments arranged by size:
1871 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 281 1541 281
Size: 1260 2369
BsrFI r'CCGG_y
Cuts at: 1627 1808 2017 1627
Size: 181 209 3239
Fragments arranged by size:
3239 209 181
BssSI C'ACGA_G
Cuts at: 1900 2258 1900
Size: 358 3271
BstAPI GCAn_nnn'nTGC
Cuts at: 1006 1006
Size: 3629
BstBI TT'CG_AA
Cuts at: 1990 1990
Size: 3629
BstXI CCAn_nnnn'nTGG
Cuts at: 504 504
Size: 3629
BstYI r'GATC_y
Cuts at: 1 203 654 1121 1144 1479 1725 2737 2823
Size: 202 451 467 23 335 246 1012 86
Cuts at: 2823 2835 3181 1
Size: 12 346 449
Fragments arranged by size:
1012 467 451 449 346 335 246 202
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3629
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1579 1760 1923 1579
Size: 181 163 3285
Fragments arranged by size:
3285 181 163
BtsI GCAGTG_nn'
Cuts at: 872 872
Size: 3629
DraI TTT'AAA
Cuts at: 123 628 123
Size: 505 3124
DrdI GACnn_nn'nnGTC
Cuts at: 1335 2193 1335
Size: 858 2771
EaeI y'GGCC_r
Cuts at: 494 1214 1388 1779 1806 3151 494
Size: 720 174 391 27 1345 972
Fragments arranged by size:
1345 972 720 391 174 27
EagI C'GGCC_G
Cuts at: 1214 1214
Size: 3629
EarI CTCTTCn'nnn_
Cuts at: 182 1652 1862 182
Size: 1470 210 1949
Fragments arranged by size:
1949 1470 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1837 2147 2293 3439 1837
Size: 310 146 1146 2027
Fragments arranged by size:
2027 1146 310 146
EcoICRI GAG'CTC
Cuts at: 641 641
Size: 3629
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 206 286 1454 1886 2633 2901 206
Size: 80 1168 432 747 268 934
Fragments arranged by size:
1168 934 747 432 268 80
EcoO109I rG'GnC_Cy
Cuts at: 281 282 768 281
Size: 1 486 3142
Fragments arranged by size:
3142 486 1
EcoRI G'AATT_C
Cuts at: 633 633
Size: 3629
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1648 1648
Size: 3629
FspI TGC'GCA
Cuts at: 598 1410 598
Size: 812 2817
HaeII r_GCGC'y
Cuts at: 1063 1311 2333 3312 1063
Size: 248 1022 979 1380
Fragments arranged by size:
1380 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1471 1501 1503 1533 1471
Size: 30 2 30 3567
Fragments arranged by size:
3567 30 30 2
HincII GTy'rAC
Cuts at: 668 668
Size: 3629
HindIII A'AGCT_T
Cuts at: 212 431 212
Size: 219 3410
KasI G'GCGC_C
Cuts at: 1059 1307 1059
Size: 248 3381
KpnI G_GTAC'C
Cuts at: 649 649
Size: 3629
MluI A'CGCG_T
Cuts at: 489 3062 489
Size: 2573 1056
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 140 2300 2484 140
Size: 2160 184 1285
Fragments arranged by size:
2160 1285 184
MscI TGG'CCA
Cuts at: 496 1390 496
Size: 894 2735
MslI CAynn'nnrTG
Cuts at: 1618 1745 1618
Size: 127 3502
MspA1I CmG'CkG
Cuts at: 740 1054 1414 2427 2672 740
Size: 314 360 1013 245 1697
Fragments arranged by size:
1697 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1810 1810
Size: 3629
NarI GG'CG_CC
Cuts at: 1060 1308 1060
Size: 248 3381
NcoI C'CATG_G
Cuts at: 107 107
Size: 3629
NgoMIV G'CCGG_C
Cuts at: 1808 1808
Size: 3629
NspI r_CATG'y
Cuts at: 682 1182 2089 682
Size: 500 907 2222
Fragments arranged by size:
2222 907 500
PasI CC'CwG_GG
Cuts at: 285 285
Size: 3629
PciI A'CATG_T
Cuts at: 2085 2085
Size: 3629
PfoI T'CCnGG_A
Cuts at: 206 206
Size: 3629
PmeI GTTT'AAAC
Cuts at: 628 628
Size: 3629
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 574 606 2768 2800 574
Size: 32 2162 32 1403
Fragments arranged by size:
2162 1403 32 32
PspOMI G'GGCC_C
Cuts at: 281 281
Size: 3629
PstI C_TGCA'G
Cuts at: 536 676 1361 536
Size: 140 685 2804
Fragments arranged by size:
2804 685 140
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3629
PvuII CAG'CTG
Cuts at: 1054 1414 1054
Size: 360 3269
RsrII CG'GwC_CG
Cuts at: 1824 1824
Size: 3629
SacI G_AGCT'C
Cuts at: 643 643
Size: 3629
SalI G'TCGA_C
Cuts at: 666 666
Size: 3629
SapI GCTCTTCn'nnn_
Cuts at: 182 1652 1862 182
Size: 1470 210 1949
Fragments arranged by size:
1949 1470 210
SbfI CC_TGCA'GG
Cuts at: 676 676
Size: 3629
ScaI AGT'ACT
Cuts at: 3146 3146
Size: 3629
SfcI C'TryA_G
Cuts at: 33 532 672 1357 2350 2541 33
Size: 499 140 685 993 191 1121
Fragments arranged by size:
1121 993 685 499 191 140
SfoI GGC'GCC
Cuts at: 1061 1309 1061
Size: 248 3381
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3629
SmaI CCC'GGG
Cuts at: 651 651
Size: 3629
SmlI C'TyrA_G
Cuts at: 784 1699 2191 2453 784
Size: 915 492 262 1960
Fragments arranged by size:
1960 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3629
SphI G_CATG'C
Cuts at: 682 1182 682
Size: 500 3129
StyI C'CwwG_G
Cuts at: 107 497 763 1117 107
Size: 390 266 354 2619
Fragments arranged by size:
2619 390 354 266
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 496 1212 1345 1804 2013 496
Size: 716 133 459 209 2112
Fragments arranged by size:
2112 716 459 209 133
TatI w'GTAC_w
Cuts at: 3144 3144
Size: 3629
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3146 3178 3146
Size: 32 3597
Tth111I GACn'n_nGTC
Cuts at: 1426 1426
Size: 3629
XbaI T'CTAG_A
Cuts at: 660 660
Size: 3629
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1124 1124
Size: 3629
XmaI C'CCGG_G
Cuts at: 649 649
Size: 3629
XmnI GAAnn'nnTTC
Cuts at: 433 433
Size: 3629
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PfoI PmeI PpiI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI BpmI BsaXI BsgI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PflMI PmlI PpuMI PshAI
PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI ZraI