Restriction map for
pF1KE0143
(Circular) (Six-base) MAPSORT of: pF1KE0143.seq Check: 9172 from: 1 to: 4043
pF1KE0143 4043 bp
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AccI GT'mk_AC
Cuts at: 1081 1081
Size: 4043
Acc65I G'GTAC_C
Cuts at: 270 1059 270
Size: 789 3254
AclI AA'CG_TT
Cuts at: 3883 3883
Size: 4043
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1868 2300 3047 3315 1868
Size: 432 747 268 2596
Fragments arranged by size:
2596 747 432 268
AflIII A'CryG_T
Cuts at: 1395 2499 3476 1395
Size: 1104 977 1962
Fragments arranged by size:
1962 1104 977
AgeI A'CCGG_T
Cuts at: 2431 2431
Size: 4043
AlwNI CAG_nnn'CTG
Cuts at: 1348 2915 1348
Size: 1567 2476
ApaI G_GGCC'C
Cuts at: 247 261 247
Size: 14 4029
ApaLI G'TGCA_C
Cuts at: 2813 2813
Size: 4043
ApoI r'AATT_y
Cuts at: 408 1047 3396 3652 408
Size: 639 2349 256 799
Fragments arranged by size:
2349 799 639 256
AseI AT'TA_AT
Cuts at: 20 899 20
Size: 879 3164
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4043
AvaI C'yCGr_G
Cuts at: 201 1063 201
Size: 862 3181
BamHI G'GATC_C
Cuts at: 145 1068 145
Size: 923 3120
BanI G'GyrC_C
Cuts at: 129 163 270 347 1059 1473 1721 1756 129
Size: 34 107 77 712 414 248 35 2416
Fragments arranged by size:
2416 712 414 248 107 77 35 34
BanII G_rGCy'C
Cuts at: 247 261 728 1057 2087 247
Size: 14 467 329 1030 2203
Fragments arranged by size:
2203 1030 467 329 14
BbeI G_GCGC'C
Cuts at: 167 1477 1725 167
Size: 1310 248 2485
Fragments arranged by size:
2485 1310 248
BbsI GAAGACnn'nnnn_
Cuts at: 328 985 328
Size: 657 3386
BciVI GTATCCnnnnn_n'
Cuts at: 1941 2702 4043 1941
Size: 761 1341 1941
Fragments arranged by size:
1941 1341 761
BclI T'GATC_A
Cuts at: 1563 1563
Size: 4043
BglI GCCn_nnn'nGGC
Cuts at: 112 121 112
Size: 9 4034
BglII A'GATC_T
Cuts at: 1558 3595 1558
Size: 2037 2006
BlpI GC'TnA_GC
Cuts at: 1155 1155
Size: 4043
Bme1580I G_kGCm'C
Cuts at: 247 261 1668 1761 2817 247
Size: 14 1407 93 1056 1473
Fragments arranged by size:
1473 1407 1056 93 14
BmrI ACTGGGnnnn_n'
Cuts at: 1255 1336 1671 3815 1255
Size: 81 335 2144 1483
Fragments arranged by size:
2144 1483 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2410 2410
Size: 4043
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 373 373
Size: 4043
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1219 2098 2590 2888 1219
Size: 879 492 298 2374
Fragments arranged by size:
2374 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3747 3747
Size: 4043
BsaAI yAC'GTr
Cuts at: 1396 1396
Size: 4043
BsaBI GATnn'nnATC
Cuts at: 6 1557 3594 6
Size: 1551 2037 455
Fragments arranged by size:
2037 1551 455
BsaHI Gr'CG_yC
Cuts at: 164 1474 1722 2424 3920 164
Size: 1310 248 702 1496 287
Fragments arranged by size:
1496 1310 702 287 248
BsaWI w'CCGG_w
Cuts at: 1232 1753 2431 2705 2852 3263 1232
Size: 521 678 274 147 411 2012
Fragments arranged by size:
2012 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 370 373 370
Size: 3 4040
BseYI C'CCAG_C
Cuts at: 266 1468 2226 2803 3299 266
Size: 1202 758 577 496 1010
Fragments arranged by size:
1202 1010 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 116 281 1585 1631 2839 104
Size: 12 165 1304 46 1208 1308
Fragments arranged by size:
1308 1304 1208 165 46 12
BsiHKAI G_wGCw'C
Cuts at: 728 1057 1835 2025 2817 728
Size: 329 778 190 792 1954
Fragments arranged by size:
1954 792 778 329 190
BsmBI CGTCTCn'nnnn_
Cuts at: 806 806
Size: 4043
Bsp1286I G_dGCh'C
Cuts at: 247 261 728 1057 1668 1761 1835 2025 2087
Size: 14 467 329 611 93 74 190 62
Cuts at: 2087 2817 247
Size: 730 1473
Fragments arranged by size:
1473 730 611 467 329 190 93 74
62 14
BspEI T'CCGG_A
Cuts at: 3263 3263
Size: 4043
BspHI T'CATG_A
Cuts at: 3219 3219
Size: 4043
BspMI ACCTGCnnnn'nnnn_
Cuts at: 282 1093 1307 1609 1990 282
Size: 811 214 302 381 2335
Fragments arranged by size:
2335 811 381 302 214
BsrBI CCG'CTC
Cuts at: 50 241 2335 2389 3873 4038 50
Size: 191 2094 54 1484 165 55
Fragments arranged by size:
2094 1484 191 165 55 54
BsrDI GCAATG_nn'
Cuts at: 881 1955 881
Size: 1074 2969
BsrFI r'CCGG_y
Cuts at: 233 2041 2222 2431 233
Size: 1808 181 209 1845
Fragments arranged by size:
1845 1808 209 181
BssSI C'ACGA_G
Cuts at: 2314 2672 2314
Size: 358 3685
BstAPI GCAn_nnn'nTGC
Cuts at: 1420 1420
Size: 4043
BstBI TT'CG_AA
Cuts at: 2404 2404
Size: 4043
BstXI CCAn_nnnn'nTGG
Cuts at: 771 771
Size: 4043
BstYI r'GATC_y
Cuts at: 1 145 1068 1535 1558 1893 2139 3151 3237
Size: 144 923 467 23 335 246 1012 86
Cuts at: 3237 3249 3595 1
Size: 12 346 449
Fragments arranged by size:
1012 923 467 449 346 335 246 144
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 860 860
Size: 4043
BtgI C'CryG_G
Cuts at: 107 116 107
Size: 9 4034
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1993 2174 2337 1993
Size: 181 163 3699
Fragments arranged by size:
3699 181 163
BtsI GCAGTG_nn'
Cuts at: 192 776 1286 192
Size: 584 510 2949
Fragments arranged by size:
2949 584 510
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 431 466 863 898 431
Size: 35 397 35 3576
Fragments arranged by size:
3576 397 35 35
DraI TTT'AAA
Cuts at: 1005 1042 1005
Size: 37 4006
DrdI GACnn_nn'nnGTC
Cuts at: 989 1749 2607 989
Size: 760 858 2425
Fragments arranged by size:
2425 858 760
EaeI y'GGCC_r
Cuts at: 113 176 278 716 935 1628 1802 2193 2220
Size: 63 102 438 219 693 174 391 27
Cuts at: 2220 3565 113
Size: 1345 591
Fragments arranged by size:
1345 693 591 438 391 219 174 102
63 27
EagI C'GGCC_G
Cuts at: 113 278 1628 113
Size: 165 1350 2528
Fragments arranged by size:
2528 1350 165
EarI CTCTTCn'nnn_
Cuts at: 2066 2276 2066
Size: 210 3833
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2251 2561 2707 3853 2251
Size: 310 146 1146 2441
Fragments arranged by size:
2441 1146 310 146
EcoICRI GAG'CTC
Cuts at: 726 1055 726
Size: 329 3714
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 373 1868 2300 3047 3315 373
Size: 1495 432 747 268 1101
Fragments arranged by size:
1495 1101 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 355 671 718 355
Size: 316 47 3680
Fragments arranged by size:
3680 316 47
EcoO109I rG'GnC_Cy
Cuts at: 137 286 865 1016 1182 137
Size: 149 579 151 166 2998
Fragments arranged by size:
2998 579 166 151 149
EcoRI G'AATT_C
Cuts at: 1047 1047
Size: 4043
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1000 1012 2062 1000
Size: 12 1050 2981
Fragments arranged by size:
2981 1050 12
FspI TGC'GCA
Cuts at: 1824 1824
Size: 4043
HaeII r_GCGC'y
Cuts at: 167 1477 1725 2747 3726 167
Size: 1310 248 1022 979 484
Fragments arranged by size:
1310 1022 979 484 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1885 1915 1917 1947 1885
Size: 30 2 30 3981
Fragments arranged by size:
3981 30 30 2
HincII GTy'rAC
Cuts at: 1082 1082
Size: 4043
HindIII A'AGCT_T
Cuts at: 947 947
Size: 4043
KasI G'GCGC_C
Cuts at: 163 1473 1721 163
Size: 1310 248 2485
Fragments arranged by size:
2485 1310 248
KpnI G_GTAC'C
Cuts at: 274 1063 274
Size: 789 3254
MfeI C'AATT_G
Cuts at: 545 545
Size: 4043
MluI A'CGCG_T
Cuts at: 3476 3476
Size: 4043
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 994 2714 2898 994
Size: 1720 184 2139
Fragments arranged by size:
2139 1720 184
MscI TGG'CCA
Cuts at: 718 937 1804 718
Size: 219 867 2957
Fragments arranged by size:
2957 867 219
MslI CAynn'nnrTG
Cuts at: 706 2032 2159 706
Size: 1326 127 2590
Fragments arranged by size:
2590 1326 127
MspA1I CmG'CkG
Cuts at: 118 1154 1468 1828 2841 3086 118
Size: 1036 314 360 1013 245 1075
Fragments arranged by size:
1075 1036 1013 360 314 245
NaeI GCC'GGC
Cuts at: 235 2224 235
Size: 1989 2054
NarI GG'CG_CC
Cuts at: 164 1474 1722 164
Size: 1310 248 2485
Fragments arranged by size:
2485 1310 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4043
NgoMIV G'CCGG_C
Cuts at: 233 2222 233
Size: 1989 2054
NotI GC'GGCC_GC
Cuts at: 113 113
Size: 4043
NspI r_CATG'y
Cuts at: 157 1096 1596 2503 157
Size: 939 500 907 1697
Fragments arranged by size:
1697 939 907 500
PasI CC'CwG_GG
Cuts at: 261 508 261
Size: 247 3796
PciI A'CATG_T
Cuts at: 2499 2499
Size: 4043
PflMI CCAn_nnn'nTGG
Cuts at: 792 792
Size: 4043
PmeI GTTT'AAAC
Cuts at: 1042 1042
Size: 4043
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3182 3214 3182
Size: 32 4011
PpuMI rG'GwC_Cy
Cuts at: 286 865 286
Size: 579 3464
PsiI TTA'TAA
Cuts at: 430 430
Size: 4043
PspOMI G'GGCC_C
Cuts at: 243 257 243
Size: 14 4029
PstI C_TGCA'G
Cuts at: 1090 1775 1090
Size: 685 3358
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4043
PvuII CAG'CTG
Cuts at: 1468 1828 1468
Size: 360 3683
RsrII CG'GwC_CG
Cuts at: 2238 2238
Size: 4043
SacI G_AGCT'C
Cuts at: 728 1057 728
Size: 329 3714
SacII CC_GC'GG
Cuts at: 119 119
Size: 4043
SalI G'TCGA_C
Cuts at: 1080 1080
Size: 4043
SanDI GG'GwC_CC
Cuts at: 286 286
Size: 4043
SapI GCTCTTCn'nnn_
Cuts at: 2066 2276 2066
Size: 210 3833
SbfI CC_TGCA'GG
Cuts at: 1090 1090
Size: 4043
ScaI AGT'ACT
Cuts at: 3560 3560
Size: 4043
SfcI C'TryA_G
Cuts at: 33 306 822 1086 1771 2764 2955 33
Size: 273 516 264 685 993 191 1121
Fragments arranged by size:
1121 993 685 516 273 264 191
SfoI GGC'GCC
Cuts at: 165 1475 1723 165
Size: 1310 248 2485
Fragments arranged by size:
2485 1310 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4043
SmaI CCC'GGG
Cuts at: 203 1065 203
Size: 862 3181
SmlI C'TyrA_G
Cuts at: 1198 2113 2605 2867 1198
Size: 915 492 262 2374
Fragments arranged by size:
2374 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4043
SphI G_CATG'C
Cuts at: 157 1096 1596 157
Size: 939 500 2604
Fragments arranged by size:
2604 939 500
StuI AGG'CCT
Cuts at: 832 832
Size: 4043
StyI C'CwwG_G
Cuts at: 107 140 719 833 1177 1531 107
Size: 33 579 114 344 354 2619
Fragments arranged by size:
2619 579 354 344 114 33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1626 1759 2218 2427 1626
Size: 133 459 209 3242
Fragments arranged by size:
3242 459 209 133
TatI w'GTAC_w
Cuts at: 3558 3558
Size: 4043
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 362 394 504 536 3560 3592 362
Size: 32 110 32 3024 32 813
Fragments arranged by size:
3024 813 110 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1840 1840
Size: 4043
XbaI T'CTAG_A
Cuts at: 1074 1074
Size: 4043
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1538 1538
Size: 4043
XmaI C'CCGG_G
Cuts at: 201 1063 201
Size: 862 3181
XmnI GAAnn'nnTTC
Cuts at: 992 992
Size: 4043
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PsiI PspOMI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI
Bpu10I BsaXI BsgI BsiWI BsmI BsrGI BssHII BstEII
BstZ17I ClaI DraIII EcoRV FseI FspAI HpaI NdeI
NheI NruI NsiI PacI PfoI PmlI PshAI PspXI
PsrI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI ZraI