(Circular) (Six-base) MAPSORT of: pF1KE0146.seq Check: 5610 from: 1 to: 4001 pF1KE0146 4001 bp With 182 enzymes: SgfI * April 24, 2009 13:11 .. AccI GT'mk_AC Cuts at: 1039 1039 Size: 4001 Acc65I G'GTAC_C Cuts at: 738 770 1017 738 Size: 32 247 3722 Fragments arranged by size: 3722 247 32 AclI AA'CG_TT Cuts at: 3841 3841 Size: 4001 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 569 818 1826 2258 3005 3273 569 Size: 249 1008 432 747 268 1297 Fragments arranged by size: 1297 1008 747 432 268 249 AflII C'TTAA_G Cuts at: 979 979 Size: 4001 AflIII A'CryG_T Cuts at: 1353 2457 3434 1353 Size: 1104 977 1920 Fragments arranged by size: 1920 1104 977 AgeI A'CCGG_T Cuts at: 2389 2389 Size: 4001 AlwNI CAG_nnn'CTG Cuts at: 1306 2873 1306 Size: 1567 2434 ApaI G_GGCC'C Cuts at: 138 144 138 Size: 6 3995 ApaLI G'TGCA_C Cuts at: 2771 2771 Size: 4001 ApoI r'AATT_y Cuts at: 790 1005 3354 3610 790 Size: 215 2349 256 1181 Fragments arranged by size: 2349 1181 256 215 AseI AT'TA_AT Cuts at: 20 20 Size: 4001 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4001 AvaI C'yCGr_G Cuts at: 137 1021 137 Size: 884 3117 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 397 430 397 Size: 33 3968 BamHI G'GATC_C Cuts at: 780 888 1026 780 Size: 108 138 3755 Fragments arranged by size: 3755 138 108 BanI G'GyrC_C Cuts at: 738 770 1017 1431 1679 1714 738 Size: 32 247 414 248 35 3025 Fragments arranged by size: 3025 414 248 247 35 32 BanII G_rGCy'C Cuts at: 138 144 1015 2045 138 Size: 6 871 1030 2094 Fragments arranged by size: 2094 1030 871 6 BbeI G_GCGC'C Cuts at: 1435 1683 1435 Size: 248 3753 BbsI GAAGACnn'nnnn_ Cuts at: 228 228 Size: 4001 BciVI GTATCCnnnnn_n' Cuts at: 1899 2660 4001 1899 Size: 761 1341 1899 Fragments arranged by size: 1899 1341 761 BclI T'GATC_A Cuts at: 1521 1521 Size: 4001 BglII A'GATC_T Cuts at: 1516 3553 1516 Size: 2037 1964 BlpI GC'TnA_GC Cuts at: 1113 1113 Size: 4001 Bme1580I G_kGCm'C Cuts at: 138 144 1626 1719 2775 138 Size: 6 1482 93 1056 1364 Fragments arranged by size: 1482 1364 1056 93 6 BmrI ACTGGGnnnn_n' Cuts at: 1213 1294 1629 3773 1213 Size: 81 335 2144 1441 Fragments arranged by size: 2144 1441 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2368 2368 Size: 4001 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 902 902 Size: 4001 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1177 2056 2548 2846 1177 Size: 879 492 298 2332 Fragments arranged by size: 2332 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 3705 3705 Size: 4001 BsaAI yAC'GTr Cuts at: 1354 1354 Size: 4001 BsaBI GATnn'nnATC Cuts at: 6 971 1515 3552 6 Size: 965 544 2037 455 Fragments arranged by size: 2037 965 544 455 BsaHI Gr'CG_yC Cuts at: 378 1432 1680 2382 3878 378 Size: 1054 248 702 1496 501 Fragments arranged by size: 1496 1054 702 501 248 BsaWI w'CCGG_w Cuts at: 213 1190 1711 2389 2663 2810 3221 213 Size: 977 521 678 274 147 411 993 Fragments arranged by size: 993 977 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 228 258 228 Size: 30 3971 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 547 547 Size: 4001 BseYI C'CCAG_C Cuts at: 464 1426 2184 2761 3257 464 Size: 962 758 577 496 1208 Fragments arranged by size: 1208 962 758 577 496 BsiEI CG_ry'CG Cuts at: 104 1543 1589 2797 104 Size: 1439 46 1208 1308 Fragments arranged by size: 1439 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 1015 1793 1983 2775 1015 Size: 778 190 792 2241 Fragments arranged by size: 2241 792 778 190 BsmI GAATG_Cn' Cuts at: 722 722 Size: 4001 Bsp1286I G_dGCh'C Cuts at: 138 144 1015 1626 1719 1793 1983 2045 2775 Size: 6 871 611 93 74 190 62 730 Cuts at: 2775 138 Size: 1364 Fragments arranged by size: 1364 871 730 611 190 93 74 62 6 BspEI T'CCGG_A Cuts at: 3221 3221 Size: 4001 BspHI T'CATG_A Cuts at: 3177 3177 Size: 4001 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1051 1265 1567 1948 1051 Size: 214 302 381 3104 Fragments arranged by size: 3104 381 302 214 BsrBI CCG'CTC Cuts at: 50 2293 2347 3831 3996 50 Size: 2243 54 1484 165 55 Fragments arranged by size: 2243 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 1913 1913 Size: 4001 BsrFI r'CCGG_y Cuts at: 112 1999 2180 2389 112 Size: 1887 181 209 1724 Fragments arranged by size: 1887 1724 209 181 BssSI C'ACGA_G Cuts at: 2272 2630 2272 Size: 358 3643 BstAPI GCAn_nnn'nTGC Cuts at: 1378 1378 Size: 4001 BstBI TT'CG_AA Cuts at: 2362 2362 Size: 4001 BstYI r'GATC_y Cuts at: 1 780 888 1026 1493 1516 1851 2097 3109 Size: 779 108 138 467 23 335 246 1012 Cuts at: 3109 3195 3207 3553 1 Size: 86 12 346 449 Fragments arranged by size: 1012 779 467 449 346 335 246 138 108 86 23 12 BtgI C'CryG_G Cuts at: 107 765 107 Size: 658 3343 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1951 2132 2295 1951 Size: 181 163 3657 Fragments arranged by size: 3657 181 163 BtsI GCAGTG_nn' Cuts at: 1244 1244 Size: 4001 DraI TTT'AAA Cuts at: 1000 1000 Size: 4001 DrdI GACnn_nn'nnGTC Cuts at: 230 1707 2565 230 Size: 1477 858 1666 Fragments arranged by size: 1666 1477 858 EaeI y'GGCC_r Cuts at: 110 179 938 1586 1760 2151 2178 3523 110 Size: 69 759 648 174 391 27 1345 588 Fragments arranged by size: 1345 759 648 588 391 174 69 27 EagI C'GGCC_G Cuts at: 1586 1586 Size: 4001 EarI CTCTTCn'nnn_ Cuts at: 234 868 2024 2234 234 Size: 634 1156 210 2001 Fragments arranged by size: 2001 1156 634 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 793 2209 2519 2665 3811 793 Size: 1416 310 146 1146 983 Fragments arranged by size: 1416 1146 983 310 146 EcoICRI GAG'CTC Cuts at: 1013 1013 Size: 4001 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 569 818 902 1826 2258 3005 3273 569 Size: 249 84 924 432 747 268 1297 Fragments arranged by size: 1297 924 747 432 268 249 84 EcoO109I rG'GnC_Cy Cuts at: 134 141 159 1140 134 Size: 7 18 981 2995 Fragments arranged by size: 2995 981 18 7 EcoRI G'AATT_C Cuts at: 1005 1005 Size: 4001 EcoRV GAT'ATC Cuts at: 846 846 Size: 4001 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 823 2020 823 Size: 1197 2804 FspI TGC'GCA Cuts at: 1782 1782 Size: 4001 HaeII r_GCGC'y Cuts at: 1435 1683 2705 3684 1435 Size: 248 1022 979 1752 Fragments arranged by size: 1752 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 797 829 1843 1873 1875 1905 797 Size: 32 1014 30 2 30 2893 Fragments arranged by size: 2893 1014 32 30 30 2 HincII GTy'rAC Cuts at: 448 1040 448 Size: 592 3409 KasI G'GCGC_C Cuts at: 1431 1679 1431 Size: 248 3753 KpnI G_GTAC'C Cuts at: 742 774 1021 742 Size: 32 247 3722 Fragments arranged by size: 3722 247 32 MluI A'CGCG_T Cuts at: 3434 3434 Size: 4001 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 214 2672 2856 214 Size: 2458 184 1359 Fragments arranged by size: 2458 1359 184 MscI TGG'CCA Cuts at: 181 1762 181 Size: 1581 2420 MslI CAynn'nnrTG Cuts at: 1990 2117 1990 Size: 127 3874 MspA1I CmG'CkG Cuts at: 525 1112 1426 1786 2799 3044 525 Size: 587 314 360 1013 245 1482 Fragments arranged by size: 1482 1013 587 360 314 245 NaeI GCC'GGC Cuts at: 2182 2182 Size: 4001 NarI GG'CG_CC Cuts at: 1432 1680 1432 Size: 248 3753 NcoI C'CATG_G Cuts at: 107 107 Size: 4001 NgoMIV G'CCGG_C Cuts at: 2180 2180 Size: 4001 NspI r_CATG'y Cuts at: 1054 1554 2461 1054 Size: 500 907 2594 Fragments arranged by size: 2594 907 500 PciI A'CATG_T Cuts at: 2457 2457 Size: 4001 PfoI T'CCnGG_A Cuts at: 918 918 Size: 4001 PmeI GTTT'AAAC Cuts at: 1000 1000 Size: 4001 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3140 3172 3140 Size: 32 3969 PspOMI G'GGCC_C Cuts at: 134 140 134 Size: 6 3995 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 754 786 754 Size: 32 3969 PstI C_TGCA'G Cuts at: 413 1048 1733 413 Size: 635 685 2681 Fragments arranged by size: 2681 685 635 PvuI CG_AT'CG Cuts at: 104 104 Size: 4001 PvuII CAG'CTG Cuts at: 525 1426 1786 525 Size: 901 360 2740 Fragments arranged by size: 2740 901 360 RsrII CG'GwC_CG Cuts at: 2196 2196 Size: 4001 SacI G_AGCT'C Cuts at: 1015 1015 Size: 4001 SalI G'TCGA_C Cuts at: 1038 1038 Size: 4001 SapI GCTCTTCn'nnn_ Cuts at: 2024 2234 2024 Size: 210 3791 SbfI CC_TGCA'GG Cuts at: 1048 1048 Size: 4001 ScaI AGT'ACT Cuts at: 3518 3518 Size: 4001 SfcI C'TryA_G Cuts at: 33 409 632 962 1044 1729 2722 2913 33 Size: 376 223 330 82 685 993 191 1121 Fragments arranged by size: 1121 993 685 376 330 223 191 82 SfoI GGC'GCC Cuts at: 1433 1681 1433 Size: 248 3753 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4001 SmaI CCC'GGG Cuts at: 139 1023 139 Size: 884 3117 SmlI C'TyrA_G Cuts at: 979 1156 2071 2563 2825 979 Size: 177 915 492 262 2155 Fragments arranged by size: 2155 915 492 262 177 SpeI A'CTAG_T Cuts at: 67 67 Size: 4001 SphI G_CATG'C Cuts at: 1054 1554 1054 Size: 500 3501 SrfI GCCC'GGGC Cuts at: 139 139 Size: 4001 SspI AAT'ATT Cuts at: 567 567 Size: 4001 StyI C'CwwG_G Cuts at: 107 892 1135 1489 107 Size: 785 243 354 2619 Fragments arranged by size: 2619 785 354 243 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 915 1584 1717 2176 2385 915 Size: 669 133 459 209 2531 Fragments arranged by size: 2531 669 459 209 133 TatI w'GTAC_w Cuts at: 573 877 3516 573 Size: 304 2639 1058 Fragments arranged by size: 2639 1058 304 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 914 946 3518 3550 914 Size: 32 2572 32 1365 Fragments arranged by size: 2572 1365 32 32 Tth111I GACn'n_nGTC Cuts at: 1798 1798 Size: 4001 XbaI T'CTAG_A Cuts at: 1032 1032 Size: 4001 XcmI CCAnnnn_n'nnnnTGG Cuts at: 472 1496 472 Size: 1024 2977 XmaI C'CCGG_G Cuts at: 137 1021 137 Size: 884 3117 Enzymes that do cut: AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI PfoI PmeI PpiI PspOMI PsrI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI SrfI SspI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AfeI AhdI AleI AlfI AloI AscI AvrII BbvCI BcgI BfrBI BglI BmgBI BmtI Bpu10I BsgI BsiWI BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI NsiI PacI PasI PflMI PmlI PpuMI PshAI PsiI PspXI SacII SanDI SexAI SfiI SgrAI SnaBI StuI SwaI XhoI XmnI ZraI