Restriction map for
pF1KE0147
(Circular) (Six-base) MAPSORT of: pF1KE0147.seq Check: 6677 from: 1 to: 3725
pF1KE0147 3725 bp
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AccI GT'mk_AC
Cuts at: 763 763
Size: 3725
Acc65I G'GTAC_C
Cuts at: 741 741
Size: 3725
AclI AA'CG_TT
Cuts at: 3565 3565
Size: 3725
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1550 1982 2729 2997 1550
Size: 432 747 268 2278
Fragments arranged by size:
2278 747 432 268
AfeI AGC'GCT
Cuts at: 470 470
Size: 3725
AflIII A'CryG_T
Cuts at: 1077 2181 3158 1077
Size: 1104 977 1644
Fragments arranged by size:
1644 1104 977
AgeI A'CCGG_T
Cuts at: 2113 2113
Size: 3725
AhdI GACnn_n'nnGTC
Cuts at: 253 253
Size: 3725
AlwNI CAG_nnn'CTG
Cuts at: 223 441 1030 2597 223
Size: 218 589 1567 1351
Fragments arranged by size:
1567 1351 589 218
ApaLI G'TGCA_C
Cuts at: 2495 2495
Size: 3725
ApoI r'AATT_y
Cuts at: 729 3078 3334 729
Size: 2349 256 1120
Fragments arranged by size:
2349 1120 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3725
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3725
AvaI C'yCGr_G
Cuts at: 309 745 309
Size: 436 3289
BamHI G'GATC_C
Cuts at: 750 750
Size: 3725
BanI G'GyrC_C
Cuts at: 445 741 1155 1403 1438 445
Size: 296 414 248 35 2732
Fragments arranged by size:
2732 414 296 248 35
BanII G_rGCy'C
Cuts at: 557 739 1769 557
Size: 182 1030 2513
Fragments arranged by size:
2513 1030 182
BbeI G_GCGC'C
Cuts at: 1159 1407 1159
Size: 248 3477
BbsI GAAGACnn'nnnn_
Cuts at: 582 582
Size: 3725
BciVI GTATCCnnnnn_n'
Cuts at: 1623 2384 3725 1623
Size: 761 1341 1623
Fragments arranged by size:
1623 1341 761
BclI T'GATC_A
Cuts at: 1245 1245
Size: 3725
BglI GCCn_nnn'nGGC
Cuts at: 461 461
Size: 3725
BglII A'GATC_T
Cuts at: 1240 3277 1240
Size: 2037 1688
BlpI GC'TnA_GC
Cuts at: 532 837 532
Size: 305 3420
Bme1580I G_kGCm'C
Cuts at: 265 368 448 1350 1443 2499 265
Size: 103 80 902 93 1056 1491
Fragments arranged by size:
1491 1056 902 103 93 80
BmrI ACTGGGnnnn_n'
Cuts at: 937 1018 1353 3497 937
Size: 81 335 2144 1165
Fragments arranged by size:
2144 1165 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 511 2092 511
Size: 1581 2144
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 397 506 397
Size: 109 3616
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 901 1780 2272 2570 901
Size: 879 492 298 2056
Fragments arranged by size:
2056 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3429 3429
Size: 3725
BsaAI yAC'GTr
Cuts at: 582 1078 582
Size: 496 3229
BsaBI GATnn'nnATC
Cuts at: 6 1239 3276 6
Size: 1233 2037 455
Fragments arranged by size:
2037 1233 455
BsaHI Gr'CG_yC
Cuts at: 1156 1404 2106 3602 1156
Size: 248 702 1496 1279
Fragments arranged by size:
1496 1279 702 248
BsaWI w'CCGG_w
Cuts at: 252 914 1435 2113 2387 2534 2945 252
Size: 662 521 678 274 147 411 1032
Fragments arranged by size:
1032 678 662 521 411 274 147
BseYI C'CCAG_C
Cuts at: 151 330 1150 1908 2485 2981 151
Size: 179 820 758 577 496 895
Fragments arranged by size:
895 820 758 577 496 179
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 178 236 712 178
Size: 58 476 3191
Fragments arranged by size:
3191 476 58
BsiEI CG_ry'CG
Cuts at: 104 1267 1313 2521 104
Size: 1163 46 1208 1308
Fragments arranged by size:
1308 1208 1163 46
BsiHKAI G_wGCw'C
Cuts at: 739 1517 1707 2499 739
Size: 778 190 792 1965
Fragments arranged by size:
1965 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 265 368 448 557 739 1350 1443 1517 1707
Size: 103 80 109 182 611 93 74 190
Cuts at: 1707 1769 2499 265
Size: 62 730 1491
Fragments arranged by size:
1491 730 611 190 182 109 103 93
80 74 62
BspEI T'CCGG_A
Cuts at: 2945 2945
Size: 3725
BspHI T'CATG_A
Cuts at: 2901 2901
Size: 3725
BspMI ACCTGCnnnn'nnnn_
Cuts at: 200 775 989 1291 1672 200
Size: 575 214 302 381 2253
Fragments arranged by size:
2253 575 381 302 214
BsrBI CCG'CTC
Cuts at: 50 185 2017 2071 3555 3720 50
Size: 135 1832 54 1484 165 55
Fragments arranged by size:
1832 1484 165 135 55 54
BsrDI GCAATG_nn'
Cuts at: 639 1637 639
Size: 998 2727
BsrFI r'CCGG_y
Cuts at: 269 1723 1904 2113 269
Size: 1454 181 209 1881
Fragments arranged by size:
1881 1454 209 181
BssSI C'ACGA_G
Cuts at: 1996 2354 1996
Size: 358 3367
BstAPI GCAn_nnn'nTGC
Cuts at: 223 1102 223
Size: 879 2846
BstBI TT'CG_AA
Cuts at: 2086 2086
Size: 3725
BstYI r'GATC_y
Cuts at: 1 750 1217 1240 1575 1821 2833 2919 2931
Size: 749 467 23 335 246 1012 86 12
Cuts at: 2931 3277 1
Size: 346 449
Fragments arranged by size:
1012 749 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 242 242
Size: 3725
BtgI C'CryG_G
Cuts at: 144 144
Size: 3725
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1675 1856 2019 1675
Size: 181 163 3381
Fragments arranged by size:
3381 181 163
BtsI GCAGTG_nn'
Cuts at: 221 368 968 221
Size: 147 600 2978
Fragments arranged by size:
2978 600 147
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 621 656 621
Size: 35 3690
DraI TTT'AAA
Cuts at: 724 724
Size: 3725
DrdI GACnn_nn'nnGTC
Cuts at: 1431 2289 1431
Size: 858 2867
EaeI y'GGCC_r
Cuts at: 642 1310 1484 1875 1902 3247 642
Size: 668 174 391 27 1345 1120
Fragments arranged by size:
1345 1120 668 391 174 27
EagI C'GGCC_G
Cuts at: 1310 1310
Size: 3725
EarI CTCTTCn'nnn_
Cuts at: 205 514 1748 1958 205
Size: 309 1234 210 1972
Fragments arranged by size:
1972 1234 309 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1933 2243 2389 3535 1933
Size: 310 146 1146 2123
Fragments arranged by size:
2123 1146 310 146
EcoICRI GAG'CTC
Cuts at: 737 737
Size: 3725
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 397 506 1550 1982 2729 2997 397
Size: 109 1044 432 747 268 1125
Fragments arranged by size:
1125 1044 747 432 268 109
EcoO109I rG'GnC_Cy
Cuts at: 247 314 438 864 247
Size: 67 124 426 3108
Fragments arranged by size:
3108 426 124 67
EcoRI G'AATT_C
Cuts at: 729 729
Size: 3725
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1744 1744
Size: 3725
FspI TGC'GCA
Cuts at: 1506 1506
Size: 3725
HaeII r_GCGC'y
Cuts at: 472 1159 1407 2429 3408 472
Size: 687 248 1022 979 789
Fragments arranged by size:
1022 979 789 687 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 108 140 239 271 1567 1597 1599 1629 108
Size: 32 99 32 1296 30 2 30 2204
Fragments arranged by size:
2204 1296 99 32 32 30 30 2
HincII GTy'rAC
Cuts at: 764 764
Size: 3725
KasI G'GCGC_C
Cuts at: 1155 1403 1155
Size: 248 3477
KpnI G_GTAC'C
Cuts at: 745 745
Size: 3725
MluI A'CGCG_T
Cuts at: 3158 3158
Size: 3725
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 271 389 2396 2580 271
Size: 118 2007 184 1416
Fragments arranged by size:
2007 1416 184 118
MscI TGG'CCA
Cuts at: 644 1486 644
Size: 842 2883
MslI CAynn'nnrTG
Cuts at: 1714 1841 1714
Size: 127 3598
MspA1I CmG'CkG
Cuts at: 137 146 417 484 836 1150 1510 2523 2768
Size: 9 271 67 352 314 360 1013 245
Cuts at: 2768 137
Size: 1094
Fragments arranged by size:
1094 1013 360 352 314 271 245 67
9
NaeI GCC'GGC
Cuts at: 271 1906 271
Size: 1635 2090
NarI GG'CG_CC
Cuts at: 1156 1404 1156
Size: 248 3477
NgoMIV G'CCGG_C
Cuts at: 269 1904 269
Size: 1635 2090
NspI r_CATG'y
Cuts at: 778 1278 2185 778
Size: 500 907 2318
Fragments arranged by size:
2318 907 500
PasI CC'CwG_GG
Cuts at: 441 441
Size: 3725
PciI A'CATG_T
Cuts at: 2181 2181
Size: 3725
PflMI CCAn_nnn'nTGG
Cuts at: 441 441
Size: 3725
PmeI GTTT'AAAC
Cuts at: 724 724
Size: 3725
PmlI CAC'GTG
Cuts at: 582 582
Size: 3725
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 382 414 2864 2896 382
Size: 32 2450 32 1211
Fragments arranged by size:
2450 1211 32 32
PpuMI rG'GwC_Cy
Cuts at: 247 314 247
Size: 67 3658
PstI C_TGCA'G
Cuts at: 136 142 772 1457 136
Size: 6 630 685 2404
Fragments arranged by size:
2404 685 630 6
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3725
PvuII CAG'CTG
Cuts at: 137 484 1150 1510 137
Size: 347 666 360 2352
Fragments arranged by size:
2352 666 360 347
RsrII CG'GwC_CG
Cuts at: 1920 1920
Size: 3725
SacI G_AGCT'C
Cuts at: 739 739
Size: 3725
SacII CC_GC'GG
Cuts at: 147 147
Size: 3725
SalI G'TCGA_C
Cuts at: 762 762
Size: 3725
SanDI GG'GwC_CC
Cuts at: 314 314
Size: 3725
SapI GCTCTTCn'nnn_
Cuts at: 1748 1958 1748
Size: 210 3515
SbfI CC_TGCA'GG
Cuts at: 772 772
Size: 3725
ScaI AGT'ACT
Cuts at: 3242 3242
Size: 3725
SfcI C'TryA_G
Cuts at: 33 132 138 768 1453 2446 2637 33
Size: 99 6 630 685 993 191 1121
Fragments arranged by size:
1121 993 685 630 191 99 6
SfoI GGC'GCC
Cuts at: 1157 1405 1157
Size: 248 3477
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3725
SmaI CCC'GGG
Cuts at: 747 747
Size: 3725
SmlI C'TyrA_G
Cuts at: 880 1795 2287 2549 880
Size: 915 492 262 2056
Fragments arranged by size:
2056 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3725
SphI G_CATG'C
Cuts at: 778 1278 778
Size: 500 3225
StyI C'CwwG_G
Cuts at: 545 557 859 1213 545
Size: 12 302 354 3057
Fragments arranged by size:
3057 354 302 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 322 636 1308 1441 1900 2109 322
Size: 314 672 133 459 209 1938
Fragments arranged by size:
1938 672 459 314 209 133
TatI w'GTAC_w
Cuts at: 3240 3240
Size: 3725
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 154 186 611 643 3242 3274 154
Size: 32 425 32 2599 32 605
Fragments arranged by size:
2599 605 425 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1522 1522
Size: 3725
XbaI T'CTAG_A
Cuts at: 756 756
Size: 3725
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1220 1220
Size: 3725
XmaI C'CCGG_G
Cuts at: 745 745
Size: 3725
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PasI PciI PflMI PmeI PmlI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AflII AleI AlfI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI Bpu10I
BsaXI BseRI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I ClaI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NcoI NdeI NheI NotI NruI
NsiI PacI PfoI PshAI PsiI PspOMI PspXI PsrI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI