Restriction map for
pF1KE0154
(Circular) (Six-base) MAPSORT of: pF1KE0154.seq Check: 7613 from: 1 to: 4007
pF1KE0154 4007 bp
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 253 253
Size: 4007
AccI GT'mk_AC
Cuts at: 1045 1045
Size: 4007
Acc65I G'GTAC_C
Cuts at: 173 1023 173
Size: 850 3157
AclI AA'CG_TT
Cuts at: 3847 3847
Size: 4007
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1832 2264 3011 3279 1832
Size: 432 747 268 2560
Fragments arranged by size:
2560 747 432 268
AflIII A'CryG_T
Cuts at: 1359 2463 3440 1359
Size: 1104 977 1926
Fragments arranged by size:
1926 1104 977
AgeI A'CCGG_T
Cuts at: 2395 2395
Size: 4007
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 335 335
Size: 4007
AlwNI CAG_nnn'CTG
Cuts at: 1312 2879 1312
Size: 1567 2440
ApaLI G'TGCA_C
Cuts at: 2777 2777
Size: 4007
ApoI r'AATT_y
Cuts at: 288 775 1011 3360 3616 288
Size: 487 236 2349 256 679
Fragments arranged by size:
2349 679 487 256 236
AseI AT'TA_AT
Cuts at: 20 20
Size: 4007
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4007
AvaI C'yCGr_G
Cuts at: 1027 1027
Size: 4007
BamHI G'GATC_C
Cuts at: 1032 1032
Size: 4007
BanI G'GyrC_C
Cuts at: 115 173 1023 1437 1685 1720 115
Size: 58 850 414 248 35 2402
Fragments arranged by size:
2402 850 414 248 58 35
BanII G_rGCy'C
Cuts at: 1021 2051 1021
Size: 1030 2977
BbeI G_GCGC'C
Cuts at: 119 1441 1689 119
Size: 1322 248 2437
Fragments arranged by size:
2437 1322 248
BbsI GAAGACnn'nnnn_
Cuts at: 1001 1001
Size: 4007
BbvCI CC'TCA_GC
Cuts at: 625 625
Size: 4007
BciVI GTATCCnnnnn_n'
Cuts at: 572 605 1905 2666 4007 572
Size: 33 1300 761 1341 572
Fragments arranged by size:
1341 1300 761 572 33
BclI T'GATC_A
Cuts at: 1527 1527
Size: 4007
BglII A'GATC_T
Cuts at: 1522 3559 1522
Size: 2037 1970
BlpI GC'TnA_GC
Cuts at: 1119 1119
Size: 4007
Bme1580I G_kGCm'C
Cuts at: 684 935 1632 1725 2781 684
Size: 251 697 93 1056 1910
Fragments arranged by size:
1910 1056 697 251 93
BmrI ACTGGGnnnn_n'
Cuts at: 495 1219 1300 1635 3779 495
Size: 724 81 335 2144 723
Fragments arranged by size:
2144 724 723 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2374 2374
Size: 4007
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 331 495 331
Size: 164 3843
Bpu10I CC'TnA_GC
Cuts at: 625 625
Size: 4007
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1183 2062 2554 2852 1183
Size: 879 492 298 2338
Fragments arranged by size:
2338 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3711 3711
Size: 4007
BsaAI yAC'GTr
Cuts at: 219 492 1360 219
Size: 273 868 2866
Fragments arranged by size:
2866 868 273
BsaBI GATnn'nnATC
Cuts at: 6 1521 3558 6
Size: 1515 2037 455
Fragments arranged by size:
2037 1515 455
BsaHI Gr'CG_yC
Cuts at: 116 1438 1686 2388 3884 116
Size: 1322 248 702 1496 239
Fragments arranged by size:
1496 1322 702 248 239
BsaWI w'CCGG_w
Cuts at: 1196 1717 2395 2669 2816 3227 1196
Size: 521 678 274 147 411 1976
Fragments arranged by size:
1976 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 481 511 481
Size: 30 3977
BseYI C'CCAG_C
Cuts at: 260 598 1432 2190 2767 3263 260
Size: 338 834 758 577 496 1004
Fragments arranged by size:
1004 834 758 577 496 338
BsiEI CG_ry'CG
Cuts at: 104 285 1549 1595 2803 104
Size: 181 1264 46 1208 1308
Fragments arranged by size:
1308 1264 1208 181 46
BsiHKAI G_wGCw'C
Cuts at: 345 1021 1799 1989 2781 345
Size: 676 778 190 792 1571
Fragments arranged by size:
1571 792 778 676 190
BsmBI CGTCTCn'nnnn_
Cuts at: 162 162
Size: 4007
Bsp1286I G_dGCh'C
Cuts at: 345 684 935 1021 1632 1725 1799 1989 2051
Size: 339 251 86 611 93 74 190 62
Cuts at: 2051 2781 345
Size: 730 1571
Fragments arranged by size:
1571 730 611 339 251 190 93 86
74 62
BspEI T'CCGG_A
Cuts at: 3227 3227
Size: 4007
BspHI T'CATG_A
Cuts at: 482 3183 482
Size: 2701 1306
BspMI ACCTGCnnnn'nnnn_
Cuts at: 253 1057 1271 1573 1954 253
Size: 804 214 302 381 2306
Fragments arranged by size:
2306 804 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2299 2353 3837 4002 50
Size: 2249 54 1484 165 55
Fragments arranged by size:
2249 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1919 1919
Size: 4007
BsrFI r'CCGG_y
Cuts at: 2005 2186 2395 2005
Size: 181 209 3617
Fragments arranged by size:
3617 209 181
BssSI C'ACGA_G
Cuts at: 2278 2636 2278
Size: 358 3649
BstAPI GCAn_nnn'nTGC
Cuts at: 1384 1384
Size: 4007
BstBI TT'CG_AA
Cuts at: 2368 2368
Size: 4007
BstYI r'GATC_y
Cuts at: 1 1032 1499 1522 1857 2103 3115 3201 3213
Size: 1031 467 23 335 246 1012 86 12
Cuts at: 3213 3559 1
Size: 346 449
Fragments arranged by size:
1031 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 194 194
Size: 4007
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1957 2138 2301 1957
Size: 181 163 3663
Fragments arranged by size:
3663 181 163
BtsI GCAGTG_nn'
Cuts at: 255 911 939 1250 255
Size: 656 28 311 3012
Fragments arranged by size:
3012 656 311 28
DraI TTT'AAA
Cuts at: 462 614 1006 462
Size: 152 392 3463
Fragments arranged by size:
3463 392 152
DrdI GACnn_nn'nnGTC
Cuts at: 583 1713 2571 583
Size: 1130 858 2019
Fragments arranged by size:
2019 1130 858
EaeI y'GGCC_r
Cuts at: 146 282 1592 1766 2157 2184 3529 146
Size: 136 1310 174 391 27 1345 624
Fragments arranged by size:
1345 1310 624 391 174 136 27
EagI C'GGCC_G
Cuts at: 282 1592 282
Size: 1310 2697
EarI CTCTTCn'nnn_
Cuts at: 462 576 2030 2240 462
Size: 114 1454 210 2229
Fragments arranged by size:
2229 1454 210 114
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2215 2525 2671 3817 2215
Size: 310 146 1146 2405
Fragments arranged by size:
2405 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1019 1019
Size: 4007
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 331 495 1832 2264 3011 3279 331
Size: 164 1337 432 747 268 1059
Fragments arranged by size:
1337 1059 747 432 268 164
EcoO109I rG'GnC_Cy
Cuts at: 1146 1146
Size: 4007
EcoRI G'AATT_C
Cuts at: 775 1011 775
Size: 236 3771
EcoRV GAT'ATC
Cuts at: 948 948
Size: 4007
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 333 2026 333
Size: 1693 2314
FspI TGC'GCA
Cuts at: 368 1788 368
Size: 1420 2587
HaeII r_GCGC'y
Cuts at: 119 1441 1689 2711 3690 119
Size: 1322 248 1022 979 436
Fragments arranged by size:
1322 1022 979 436 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1849 1879 1881 1911 1849
Size: 30 2 30 3945
Fragments arranged by size:
3945 30 30 2
HincII GTy'rAC
Cuts at: 1046 1046
Size: 4007
KasI G'GCGC_C
Cuts at: 115 1437 1685 115
Size: 1322 248 2437
Fragments arranged by size:
2437 1322 248
KpnI G_GTAC'C
Cuts at: 177 1027 177
Size: 850 3157
MfeI C'AATT_G
Cuts at: 453 453
Size: 4007
MluI A'CGCG_T
Cuts at: 3440 3440
Size: 4007
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2678 2862 2678
Size: 184 3823
MscI TGG'CCA
Cuts at: 148 1768 148
Size: 1620 2387
MslI CAynn'nnrTG
Cuts at: 1996 2123 1996
Size: 127 3880
MspA1I CmG'CkG
Cuts at: 351 629 1118 1432 1792 2805 3050 351
Size: 278 489 314 360 1013 245 1308
Fragments arranged by size:
1308 1013 489 360 314 278 245
NaeI GCC'GGC
Cuts at: 2188 2188
Size: 4007
NarI GG'CG_CC
Cuts at: 116 1438 1686 116
Size: 1322 248 2437
Fragments arranged by size:
2437 1322 248
NcoI C'CATG_G
Cuts at: 194 194
Size: 4007
NgoMIV G'CCGG_C
Cuts at: 2186 2186
Size: 4007
NspI r_CATG'y
Cuts at: 1060 1560 2467 1060
Size: 500 907 2600
Fragments arranged by size:
2600 907 500
PciI A'CATG_T
Cuts at: 2463 2463
Size: 4007
PmeI GTTT'AAAC
Cuts at: 1006 1006
Size: 4007
PmlI CAC'GTG
Cuts at: 492 492
Size: 4007
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3146 3178 3146
Size: 32 3975
PsiI TTA'TAA
Cuts at: 665 665
Size: 4007
PstI C_TGCA'G
Cuts at: 1054 1739 1054
Size: 685 3322
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4007
PvuII CAG'CTG
Cuts at: 351 629 1432 1792 351
Size: 278 803 360 2566
Fragments arranged by size:
2566 803 360 278
RsrII CG'GwC_CG
Cuts at: 2202 2202
Size: 4007
SacI G_AGCT'C
Cuts at: 1021 1021
Size: 4007
SalI G'TCGA_C
Cuts at: 1044 1044
Size: 4007
SapI GCTCTTCn'nnn_
Cuts at: 462 2030 2240 462
Size: 1568 210 2229
Fragments arranged by size:
2229 1568 210
SbfI CC_TGCA'GG
Cuts at: 1054 1054
Size: 4007
ScaI AGT'ACT
Cuts at: 3524 3524
Size: 4007
SfcI C'TryA_G
Cuts at: 33 1050 1735 2728 2919 33
Size: 1017 685 993 191 1121
Fragments arranged by size:
1121 1017 993 685 191
SfoI GGC'GCC
Cuts at: 117 1439 1687 117
Size: 1322 248 2437
Fragments arranged by size:
2437 1322 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4007
SmaI CCC'GGG
Cuts at: 1029 1029
Size: 4007
SmlI C'TyrA_G
Cuts at: 1162 2077 2569 2831 1162
Size: 915 492 262 2338
Fragments arranged by size:
2338 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4007
SphI G_CATG'C
Cuts at: 1060 1560 1060
Size: 500 3507
StyI C'CwwG_G
Cuts at: 143 194 720 1141 1495 143
Size: 51 526 421 354 2655
Fragments arranged by size:
2655 526 421 354 51
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 252 1590 1723 2182 2391 252
Size: 1338 133 459 209 1868
Fragments arranged by size:
1868 1338 459 209 133
TatI w'GTAC_w
Cuts at: 425 711 3522 425
Size: 286 2811 910
Fragments arranged by size:
2811 910 286
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 481 513 572 604 3524 3556 481
Size: 32 59 32 2920 32 932
Fragments arranged by size:
2920 932 59 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1804 1804
Size: 4007
XbaI T'CTAG_A
Cuts at: 987 1038 987
Size: 51 3956
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1502 1502
Size: 4007
XmaI C'CCGG_G
Cuts at: 1027 1027
Size: 4007
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PmeI PmlI PpiI PsiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI ApaI AscI
AvrII BaeI BcgI BfrBI BglI BmgBI BmtI BseRI
BsgI BsiWI BsmI BsrGI BssHII BstEII BstXI BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI FseI FspAI HindIII
HpaI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PpuMI PshAI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI