Restriction map for
pF1KE0155
(Circular) (Six-base) MAPSORT of: pF1KE0155.seq Check: 5129 from: 1 to: 3842
pF1KE0155 3842 bp
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AccI GT'mk_AC
Cuts at: 880 880
Size: 3842
Acc65I G'GTAC_C
Cuts at: 858 858
Size: 3842
AclI AA'CG_TT
Cuts at: 3682 3682
Size: 3842
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 178 399 1667 2099 2846 3114 178
Size: 221 1268 432 747 268 906
Fragments arranged by size:
1268 906 747 432 268 221
AflIII A'CryG_T
Cuts at: 726 818 1194 2298 3275 726
Size: 92 376 1104 977 1293
Fragments arranged by size:
1293 1104 977 376 92
AgeI A'CCGG_T
Cuts at: 2230 2230
Size: 3842
AlwNI CAG_nnn'CTG
Cuts at: 530 1147 2714 530
Size: 617 1567 1658
Fragments arranged by size:
1658 1567 617
ApaLI G'TGCA_C
Cuts at: 2612 2612
Size: 3842
ApoI r'AATT_y
Cuts at: 145 652 846 3195 3451 145
Size: 507 194 2349 256 536
Fragments arranged by size:
2349 536 507 256 194
AseI AT'TA_AT
Cuts at: 20 20
Size: 3842
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3842
AvaI C'yCGr_G
Cuts at: 862 862
Size: 3842
BamHI G'GATC_C
Cuts at: 867 867
Size: 3842
BanI G'GyrC_C
Cuts at: 772 858 1272 1520 1555 772
Size: 86 414 248 35 3059
Fragments arranged by size:
3059 414 248 86 35
BanII G_rGCy'C
Cuts at: 856 1886 856
Size: 1030 2812
BbeI G_GCGC'C
Cuts at: 1276 1524 1276
Size: 248 3594
BbvCI CC'TCA_GC
Cuts at: 637 637
Size: 3842
BciVI GTATCCnnnnn_n'
Cuts at: 1740 2501 3842 1740
Size: 761 1341 1740
Fragments arranged by size:
1740 1341 761
BclI T'GATC_A
Cuts at: 1362 1362
Size: 3842
BglI GCCn_nnn'nGGC
Cuts at: 760 760
Size: 3842
BglII A'GATC_T
Cuts at: 1357 3394 1357
Size: 2037 1805
BlpI GC'TnA_GC
Cuts at: 954 954
Size: 3842
Bme1580I G_kGCm'C
Cuts at: 1467 1560 2616 1467
Size: 93 1056 2693
Fragments arranged by size:
2693 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1054 1135 1470 3614 1054
Size: 81 335 2144 1282
Fragments arranged by size:
2144 1282 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2209 2209
Size: 3842
Bpu10I CC'TnA_GC
Cuts at: 637 689 637
Size: 52 3790
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1018 1897 2389 2687 1018
Size: 879 492 298 2173
Fragments arranged by size:
2173 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3546 3546
Size: 3842
BsaAI yAC'GTr
Cuts at: 821 1195 821
Size: 374 3468
BsaBI GATnn'nnATC
Cuts at: 6 1356 3393 6
Size: 1350 2037 455
Fragments arranged by size:
2037 1350 455
BsaHI Gr'CG_yC
Cuts at: 497 1273 1521 2223 3719 497
Size: 776 248 702 1496 620
Fragments arranged by size:
1496 776 702 620 248
BsaWI w'CCGG_w
Cuts at: 1031 1552 2230 2504 2651 3062 1031
Size: 521 678 274 147 411 1811
Fragments arranged by size:
1811 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 808 838 808
Size: 30 3812
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 709 709
Size: 3842
BseYI C'CCAG_C
Cuts at: 200 491 1267 2025 2602 3098 200
Size: 291 776 758 577 496 944
Fragments arranged by size:
944 776 758 577 496 291
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 751 751
Size: 3842
BsiEI CG_ry'CG
Cuts at: 104 1384 1430 2638 104
Size: 1280 46 1208 1308
Fragments arranged by size:
1308 1280 1208 46
BsiHKAI G_wGCw'C
Cuts at: 856 1634 1824 2616 856
Size: 778 190 792 2082
Fragments arranged by size:
2082 792 778 190
BsmI GAATG_Cn'
Cuts at: 427 427
Size: 3842
Bsp1286I G_dGCh'C
Cuts at: 856 1467 1560 1634 1824 1886 2616 856
Size: 611 93 74 190 62 730 2082
Fragments arranged by size:
2082 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3062 3062
Size: 3842
BspHI T'CATG_A
Cuts at: 3018 3018
Size: 3842
BspMI ACCTGCnnnn'nnnn_
Cuts at: 892 1106 1408 1789 892
Size: 214 302 381 2945
Fragments arranged by size:
2945 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2134 2188 3672 3837 50
Size: 2084 54 1484 165 55
Fragments arranged by size:
2084 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 136 1754 136
Size: 1618 2224
BsrFI r'CCGG_y
Cuts at: 1840 2021 2230 1840
Size: 181 209 3452
Fragments arranged by size:
3452 209 181
BssSI C'ACGA_G
Cuts at: 2113 2471 2113
Size: 358 3484
BstAPI GCAn_nnn'nTGC
Cuts at: 1219 1219
Size: 3842
BstBI TT'CG_AA
Cuts at: 2203 2203
Size: 3842
BstXI CCAn_nnnn'nTGG
Cuts at: 834 834
Size: 3842
BstYI r'GATC_y
Cuts at: 1 867 1334 1357 1692 1938 2950 3036 3048
Size: 866 467 23 335 246 1012 86 12
Cuts at: 3048 3394 1
Size: 346 449
Fragments arranged by size:
1012 866 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 476 755 107
Size: 369 279 3194
Fragments arranged by size:
3194 369 279
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 615 1792 1973 2136 615
Size: 1177 181 163 2321
Fragments arranged by size:
2321 1177 181 163
BtsI GCAGTG_nn'
Cuts at: 1085 1085
Size: 3842
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 300 335 300
Size: 35 3807
DraI TTT'AAA
Cuts at: 841 841
Size: 3842
DrdI GACnn_nn'nnGTC
Cuts at: 1548 2406 1548
Size: 858 2984
EaeI y'GGCC_r
Cuts at: 260 614 1427 1601 1992 2019 3364 260
Size: 354 813 174 391 27 1345 738
Fragments arranged by size:
1345 813 738 391 354 174 27
EagI C'GGCC_G
Cuts at: 1427 1427
Size: 3842
EarI CTCTTCn'nnn_
Cuts at: 352 522 1865 2075 352
Size: 170 1343 210 2119
Fragments arranged by size:
2119 1343 210 170
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 792 2050 2360 2506 3652 792
Size: 1258 310 146 1146 982
Fragments arranged by size:
1258 1146 982 310 146
EcoICRI GAG'CTC
Cuts at: 854 854
Size: 3842
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 178 399 1667 2099 2846 3114 178
Size: 221 1268 432 747 268 906
Fragments arranged by size:
1268 906 747 432 268 221
EcoO109I rG'GnC_Cy
Cuts at: 710 981 710
Size: 271 3571
EcoRI G'AATT_C
Cuts at: 846 846
Size: 3842
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1861 1861
Size: 3842
FspI TGC'GCA
Cuts at: 1623 1623
Size: 3842
HaeII r_GCGC'y
Cuts at: 225 1276 1524 2546 3525 225
Size: 1051 248 1022 979 542
Fragments arranged by size:
1051 1022 979 542 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1684 1714 1716 1746 1684
Size: 30 2 30 3780
Fragments arranged by size:
3780 30 30 2
HincII GTy'rAC
Cuts at: 881 881
Size: 3842
KasI G'GCGC_C
Cuts at: 1272 1520 1272
Size: 248 3594
KpnI G_GTAC'C
Cuts at: 862 862
Size: 3842
MluI A'CGCG_T
Cuts at: 3275 3275
Size: 3842
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2513 2697 2513
Size: 184 3658
MscI TGG'CCA
Cuts at: 1603 1603
Size: 3842
MslI CAynn'nnrTG
Cuts at: 598 610 1831 1958 598
Size: 12 1221 127 2482
Fragments arranged by size:
2482 1221 127 12
MspA1I CmG'CkG
Cuts at: 156 527 953 1267 1627 2640 2885 156
Size: 371 426 314 360 1013 245 1113
Fragments arranged by size:
1113 1013 426 371 360 314 245
NaeI GCC'GGC
Cuts at: 2023 2023
Size: 3842
NarI GG'CG_CC
Cuts at: 1273 1521 1273
Size: 248 3594
NcoI C'CATG_G
Cuts at: 107 755 107
Size: 648 3194
NgoMIV G'CCGG_C
Cuts at: 2021 2021
Size: 3842
NspI r_CATG'y
Cuts at: 208 730 895 1395 2302 208
Size: 522 165 500 907 1748
Fragments arranged by size:
1748 907 522 500 165
PasI CC'CwG_GG
Cuts at: 768 768
Size: 3842
PciI A'CATG_T
Cuts at: 726 2298 726
Size: 1572 2270
PflMI CCAn_nnn'nTGG
Cuts at: 477 477
Size: 3842
PmeI GTTT'AAAC
Cuts at: 841 841
Size: 3842
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 553 585 2981 3013 553
Size: 32 2396 32 1382
Fragments arranged by size:
2396 1382 32 32
PstI C_TGCA'G
Cuts at: 526 889 1574 526
Size: 363 685 2794
Fragments arranged by size:
2794 685 363
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3842
PvuII CAG'CTG
Cuts at: 1267 1627 1267
Size: 360 3482
RsrII CG'GwC_CG
Cuts at: 2037 2037
Size: 3842
SacI G_AGCT'C
Cuts at: 856 856
Size: 3842
SalI G'TCGA_C
Cuts at: 879 879
Size: 3842
SapI GCTCTTCn'nnn_
Cuts at: 1865 2075 1865
Size: 210 3632
SbfI CC_TGCA'GG
Cuts at: 889 889
Size: 3842
ScaI AGT'ACT
Cuts at: 3359 3359
Size: 3842
SexAI A'CCwGG_T
Cuts at: 269 269
Size: 3842
SfcI C'TryA_G
Cuts at: 33 522 885 1570 2563 2754 33
Size: 489 363 685 993 191 1121
Fragments arranged by size:
1121 993 685 489 363 191
SfoI GGC'GCC
Cuts at: 1274 1522 1274
Size: 248 3594
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3842
SmaI CCC'GGG
Cuts at: 864 864
Size: 3842
SmlI C'TyrA_G
Cuts at: 997 1912 2404 2666 997
Size: 915 492 262 2173
Fragments arranged by size:
2173 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3842
SphI G_CATG'C
Cuts at: 895 1395 895
Size: 500 3342
StuI AGG'CCT
Cuts at: 394 394
Size: 3842
StyI C'CwwG_G
Cuts at: 107 755 976 1330 107
Size: 648 221 354 2619
Fragments arranged by size:
2619 648 354 221
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1425 1558 2017 2226 1425
Size: 133 459 209 3041
Fragments arranged by size:
3041 459 209 133
TatI w'GTAC_w
Cuts at: 3357 3357
Size: 3842
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3359 3391 3359
Size: 32 3810
Tth111I GACn'n_nGTC
Cuts at: 1639 1639
Size: 3842
XbaI T'CTAG_A
Cuts at: 873 873
Size: 3842
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1337 1337
Size: 3842
XmaI C'CCGG_G
Cuts at: 862 862
Size: 3842
XmnI GAAnn'nnTTC
Cuts at: 127 127
Size: 3842
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbvCI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PflMI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BcgI BfrBI BmgBI
BmtI BpmI BsiWI BsmBI BsrGI BssHII BstEII BstZ17I
Bsu36I ClaI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI ZraI