Restriction map for
pF1KE0157
(Circular) (Six-base) MAPSORT of: pF1KE0157.seq Check: 4713 from: 1 to: 3500
pF1KE0157 3500 bp
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AccI GT'mk_AC
Cuts at: 538 538
Size: 3500
Acc65I G'GTAC_C
Cuts at: 516 516
Size: 3500
AclI AA'CG_TT
Cuts at: 3340 3340
Size: 3500
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 179 1325 1757 2504 2772 179
Size: 1146 432 747 268 907
Fragments arranged by size:
1146 907 747 432 268
AflIII A'CryG_T
Cuts at: 852 1956 2933 852
Size: 1104 977 1419
Fragments arranged by size:
1419 1104 977
AgeI A'CCGG_T
Cuts at: 1888 1888
Size: 3500
AlwNI CAG_nnn'CTG
Cuts at: 275 805 2372 275
Size: 530 1567 1403
Fragments arranged by size:
1567 1403 530
ApaLI G'TGCA_C
Cuts at: 2270 2270
Size: 3500
ApoI r'AATT_y
Cuts at: 504 2853 3109 504
Size: 2349 256 895
Fragments arranged by size:
2349 895 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3500
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3500
AvaI C'yCGr_G
Cuts at: 170 520 170
Size: 350 3150
BamHI G'GATC_C
Cuts at: 525 525
Size: 3500
BanI G'GyrC_C
Cuts at: 516 930 1178 1213 516
Size: 414 248 35 2803
Fragments arranged by size:
2803 414 248 35
BanII G_rGCy'C
Cuts at: 514 1544 514
Size: 1030 2470
BbeI G_GCGC'C
Cuts at: 934 1182 934
Size: 248 3252
BbsI GAAGACnn'nnnn_
Cuts at: 422 422
Size: 3500
BciVI GTATCCnnnnn_n'
Cuts at: 1398 2159 3500 1398
Size: 761 1341 1398
Fragments arranged by size:
1398 1341 761
BclI T'GATC_A
Cuts at: 1020 1020
Size: 3500
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 3500
BglII A'GATC_T
Cuts at: 1015 3052 1015
Size: 2037 1463
BlpI GC'TnA_GC
Cuts at: 612 612
Size: 3500
Bme1580I G_kGCm'C
Cuts at: 1125 1218 2274 1125
Size: 93 1056 2351
Fragments arranged by size:
2351 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 712 793 1128 3272 712
Size: 81 335 2144 940
Fragments arranged by size:
2144 940 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1867 1867
Size: 3500
Bpu10I CC'TnA_GC
Cuts at: 236 236
Size: 3500
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 676 1555 2047 2345 676
Size: 879 492 298 1831
Fragments arranged by size:
1831 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3204 3204
Size: 3500
BsaAI yAC'GTr
Cuts at: 853 853
Size: 3500
BsaBI GATnn'nnATC
Cuts at: 6 1014 3051 6
Size: 1008 2037 455
Fragments arranged by size:
2037 1008 455
BsaHI Gr'CG_yC
Cuts at: 931 1179 1881 3377 931
Size: 248 702 1496 1054
Fragments arranged by size:
1496 1054 702 248
BsaWI w'CCGG_w
Cuts at: 689 1210 1888 2162 2309 2720 689
Size: 521 678 274 147 411 1469
Fragments arranged by size:
1469 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 341 371 341
Size: 30 3470
BseYI C'CCAG_C
Cuts at: 925 1683 2260 2756 925
Size: 758 577 496 1669
Fragments arranged by size:
1669 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1042 1088 2296 104
Size: 938 46 1208 1308
Fragments arranged by size:
1308 1208 938 46
BsiHKAI G_wGCw'C
Cuts at: 514 1292 1482 2274 514
Size: 778 190 792 1740
Fragments arranged by size:
1740 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 514 1125 1218 1292 1482 1544 2274 514
Size: 611 93 74 190 62 730 1740
Fragments arranged by size:
1740 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2720 2720
Size: 3500
BspHI T'CATG_A
Cuts at: 2676 2676
Size: 3500
BspMI ACCTGCnnnn'nnnn_
Cuts at: 550 764 1066 1447 550
Size: 214 302 381 2603
Fragments arranged by size:
2603 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1792 1846 3330 3495 50
Size: 1742 54 1484 165 55
Fragments arranged by size:
1742 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1412 1412
Size: 3500
BsrFI r'CCGG_y
Cuts at: 1498 1679 1888 1498
Size: 181 209 3110
Fragments arranged by size:
3110 209 181
BssSI C'ACGA_G
Cuts at: 1771 2129 1771
Size: 358 3142
BstAPI GCAn_nnn'nTGC
Cuts at: 877 877
Size: 3500
BstBI TT'CG_AA
Cuts at: 1861 1861
Size: 3500
BstYI r'GATC_y
Cuts at: 1 525 992 1015 1350 1596 2608 2694 2706
Size: 524 467 23 335 246 1012 86 12
Cuts at: 2706 3052 1
Size: 346 449
Fragments arranged by size:
1012 524 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1450 1631 1794 1450
Size: 181 163 3156
Fragments arranged by size:
3156 181 163
BtsI GCAGTG_nn'
Cuts at: 743 743
Size: 3500
DraI TTT'AAA
Cuts at: 499 499
Size: 3500
DrdI GACnn_nn'nnGTC
Cuts at: 1206 2064 1206
Size: 858 2642
EaeI y'GGCC_r
Cuts at: 1085 1259 1650 1677 3022 1085
Size: 174 391 27 1345 1563
Fragments arranged by size:
1563 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1085 1085
Size: 3500
EarI CTCTTCn'nnn_
Cuts at: 1523 1733 1523
Size: 210 3290
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1708 2018 2164 3310 1708
Size: 310 146 1146 1898
Fragments arranged by size:
1898 1146 310 146
EcoICRI GAG'CTC
Cuts at: 512 512
Size: 3500
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 179 1325 1757 2504 2772 179
Size: 1146 432 747 268 907
Fragments arranged by size:
1146 907 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 639 639
Size: 3500
EcoRI G'AATT_C
Cuts at: 504 504
Size: 3500
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1519 1519
Size: 3500
FspI TGC'GCA
Cuts at: 1281 1281
Size: 3500
HaeII r_GCGC'y
Cuts at: 934 1182 2204 3183 934
Size: 248 1022 979 1251
Fragments arranged by size:
1251 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 347 379 409 441 1342 1372 1374 1404 347
Size: 32 30 32 901 30 2 30 2443
Fragments arranged by size:
2443 901 32 32 30 30 30 2
HincII GTy'rAC
Cuts at: 539 539
Size: 3500
HindIII A'AGCT_T
Cuts at: 483 483
Size: 3500
KasI G'GCGC_C
Cuts at: 930 1178 930
Size: 248 3252
KpnI G_GTAC'C
Cuts at: 520 520
Size: 3500
MluI A'CGCG_T
Cuts at: 2933 2933
Size: 3500
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2171 2355 2171
Size: 184 3316
MscI TGG'CCA
Cuts at: 1261 1261
Size: 3500
MslI CAynn'nnrTG
Cuts at: 245 1489 1616 245
Size: 1244 127 2129
Fragments arranged by size:
2129 1244 127
MspA1I CmG'CkG
Cuts at: 611 925 1285 2298 2543 611
Size: 314 360 1013 245 1568
Fragments arranged by size:
1568 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1681 1681
Size: 3500
NarI GG'CG_CC
Cuts at: 931 1179 931
Size: 248 3252
NgoMIV G'CCGG_C
Cuts at: 1679 1679
Size: 3500
NspI r_CATG'y
Cuts at: 244 553 1053 1960 244
Size: 309 500 907 1784
Fragments arranged by size:
1784 907 500 309
PciI A'CATG_T
Cuts at: 1956 1956
Size: 3500
PmeI GTTT'AAAC
Cuts at: 499 499
Size: 3500
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 424 456 2639 2671 424
Size: 32 2183 32 1253
Fragments arranged by size:
2183 1253 32 32
PstI C_TGCA'G
Cuts at: 164 547 1232 164
Size: 383 685 2432
Fragments arranged by size:
2432 685 383
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3500
PvuII CAG'CTG
Cuts at: 925 1285 925
Size: 360 3140
RsrII CG'GwC_CG
Cuts at: 1695 1695
Size: 3500
SacI G_AGCT'C
Cuts at: 514 514
Size: 3500
SalI G'TCGA_C
Cuts at: 537 537
Size: 3500
SapI GCTCTTCn'nnn_
Cuts at: 1523 1733 1523
Size: 210 3290
SbfI CC_TGCA'GG
Cuts at: 547 547
Size: 3500
ScaI AGT'ACT
Cuts at: 3017 3017
Size: 3500
SfcI C'TryA_G
Cuts at: 33 160 543 1228 2221 2412 33
Size: 127 383 685 993 191 1121
Fragments arranged by size:
1121 993 685 383 191 127
SfoI GGC'GCC
Cuts at: 932 1180 932
Size: 248 3252
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3500
SmaI CCC'GGG
Cuts at: 172 522 172
Size: 350 3150
SmlI C'TyrA_G
Cuts at: 655 1570 2062 2324 655
Size: 915 492 262 1831
Fragments arranged by size:
1831 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3500
SphI G_CATG'C
Cuts at: 553 1053 553
Size: 500 3000
SspI AAT'ATT
Cuts at: 492 492
Size: 3500
StyI C'CwwG_G
Cuts at: 634 988 634
Size: 354 3146
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1083 1216 1675 1884 1083
Size: 133 459 209 2699
Fragments arranged by size:
2699 459 209 133
TatI w'GTAC_w
Cuts at: 3015 3015
Size: 3500
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3017 3049 3017
Size: 32 3468
Tth111I GACn'n_nGTC
Cuts at: 1297 1297
Size: 3500
XbaI T'CTAG_A
Cuts at: 297 531 297
Size: 234 3266
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 995 995
Size: 3500
XmaI C'CCGG_G
Cuts at: 170 520 170
Size: 350 3150
XmnI GAAnn'nnTTC
Cuts at: 338 338
Size: 3500
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PciI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI
BmtI BpmI BseRI BsgI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I BtgI ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HpaI MfeI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI StuI
SwaI XhoI ZraI