Restriction map for
pF1KE0158
(Circular) (Six-base) MAPSORT of: pF1KE0158.seq Check: 8948 from: 1 to: 3989
pF1KE0158 3989 bp
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AccI GT'mk_AC
Cuts at: 1027 1027
Size: 3989
Acc65I G'GTAC_C
Cuts at: 1005 1005
Size: 3989
AclI AA'CG_TT
Cuts at: 3829 3829
Size: 3989
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 478 803 865 1814 2246 2993 3261 478
Size: 325 62 949 432 747 268 1206
Fragments arranged by size:
1206 949 747 432 325 268 62
AflIII A'CryG_T
Cuts at: 1341 2445 3422 1341
Size: 1104 977 1908
Fragments arranged by size:
1908 1104 977
AgeI A'CCGG_T
Cuts at: 2377 2377
Size: 3989
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 425 425
Size: 3989
AlwNI CAG_nnn'CTG
Cuts at: 1294 2861 1294
Size: 1567 2422
ApaLI G'TGCA_C
Cuts at: 2759 2759
Size: 3989
ApoI r'AATT_y
Cuts at: 993 3342 3598 993
Size: 2349 256 1384
Fragments arranged by size:
2349 1384 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3989
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3989
AvaI C'yCGr_G
Cuts at: 221 338 1009 221
Size: 117 671 3201
Fragments arranged by size:
3201 671 117
BamHI G'GATC_C
Cuts at: 631 1014 631
Size: 383 3606
BanI G'GyrC_C
Cuts at: 1005 1419 1667 1702 1005
Size: 414 248 35 3292
Fragments arranged by size:
3292 414 248 35
BanII G_rGCy'C
Cuts at: 1003 2033 1003
Size: 1030 2959
BbeI G_GCGC'C
Cuts at: 1423 1671 1423
Size: 248 3741
BbsI GAAGACnn'nnnn_
Cuts at: 911 911
Size: 3989
BciVI GTATCCnnnnn_n'
Cuts at: 850 1887 2648 3989 850
Size: 1037 761 1341 850
Fragments arranged by size:
1341 1037 850 761
BclI T'GATC_A
Cuts at: 1509 1509
Size: 3989
BglII A'GATC_T
Cuts at: 876 1504 3541 876
Size: 628 2037 1324
Fragments arranged by size:
2037 1324 628
BlpI GC'TnA_GC
Cuts at: 1101 1101
Size: 3989
Bme1580I G_kGCm'C
Cuts at: 222 278 1614 1707 2763 222
Size: 56 1336 93 1056 1448
Fragments arranged by size:
1448 1336 1056 93 56
BmrI ACTGGGnnnn_n'
Cuts at: 274 1201 1282 1617 3761 274
Size: 927 81 335 2144 502
Fragments arranged by size:
2144 927 502 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2356 2356
Size: 3989
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 575 575
Size: 3989
Bpu10I CC'TnA_GC
Cuts at: 481 701 481
Size: 220 3769
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1165 2044 2536 2834 1165
Size: 879 492 298 2320
Fragments arranged by size:
2320 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 229 3693 229
Size: 3464 525
BsaAI yAC'GTr
Cuts at: 1342 1342
Size: 3989
BsaBI GATnn'nnATC
Cuts at: 6 838 1503 3540 6
Size: 832 665 2037 455
Fragments arranged by size:
2037 832 665 455
BsaHI Gr'CG_yC
Cuts at: 226 1420 1668 2370 3866 226
Size: 1194 248 702 1496 349
Fragments arranged by size:
1496 1194 702 349 248
BsaWI w'CCGG_w
Cuts at: 884 1178 1699 2377 2651 2798 3209 884
Size: 294 521 678 274 147 411 1664
Fragments arranged by size:
1664 678 521 411 294 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 340 454 694 340
Size: 114 240 3635
Fragments arranged by size:
3635 240 114
BseYI C'CCAG_C
Cuts at: 382 510 1414 2172 2749 3245 382
Size: 128 904 758 577 496 1126
Fragments arranged by size:
1126 904 758 577 496 128
BsiEI CG_ry'CG
Cuts at: 104 1531 1577 2785 104
Size: 1427 46 1208 1308
Fragments arranged by size:
1427 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1003 1781 1971 2763 1003
Size: 778 190 792 2229
Fragments arranged by size:
2229 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 804 804
Size: 3989
Bsp1286I G_dGCh'C
Cuts at: 222 278 1003 1614 1707 1781 1971 2033 2763
Size: 56 725 611 93 74 190 62 730
Cuts at: 2763 222
Size: 1448
Fragments arranged by size:
1448 730 725 611 190 93 74 62
56
BspEI T'CCGG_A
Cuts at: 3209 3209
Size: 3989
BspHI T'CATG_A
Cuts at: 3165 3165
Size: 3989
BspMI ACCTGCnnnn'nnnn_
Cuts at: 434 1039 1253 1555 1936 434
Size: 605 214 302 381 2487
Fragments arranged by size:
2487 605 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2281 2335 3819 3984 50
Size: 2231 54 1484 165 55
Fragments arranged by size:
2231 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 414 1901 414
Size: 1487 2502
BsrFI r'CCGG_y
Cuts at: 1987 2168 2377 1987
Size: 181 209 3599
Fragments arranged by size:
3599 209 181
BssSI C'ACGA_G
Cuts at: 2260 2618 2260
Size: 358 3631
BstAPI GCAn_nnn'nTGC
Cuts at: 553 1366 553
Size: 813 3176
BstBI TT'CG_AA
Cuts at: 2350 2350
Size: 3989
BstYI r'GATC_y
Cuts at: 1 631 876 1014 1481 1504 1839 2085 3097
Size: 630 245 138 467 23 335 246 1012
Cuts at: 3097 3183 3195 3541 1
Size: 86 12 346 449
Fragments arranged by size:
1012 630 467 449 346 335 246 245
138 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 934 934
Size: 3989
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 285 1939 2120 2283 285
Size: 1654 181 163 1991
Fragments arranged by size:
1991 1654 181 163
BtsI GCAGTG_nn'
Cuts at: 1232 1232
Size: 3989
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 411 446 524 559 411
Size: 35 78 35 3841
Fragments arranged by size:
3841 78 35 35
DraI TTT'AAA
Cuts at: 988 988
Size: 3989
DrdI GACnn_nn'nnGTC
Cuts at: 1695 2553 1695
Size: 858 3131
EaeI y'GGCC_r
Cuts at: 1574 1748 2139 2166 3511 1574
Size: 174 391 27 1345 2052
Fragments arranged by size:
2052 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1574 1574
Size: 3989
EarI CTCTTCn'nnn_
Cuts at: 317 615 779 2012 2222 317
Size: 298 164 1233 210 2084
Fragments arranged by size:
2084 1233 298 210 164
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2197 2507 2653 3799 2197
Size: 310 146 1146 2387
Fragments arranged by size:
2387 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1001 1001
Size: 3989
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 478 575 803 865 1814 2246 2993 3261 478
Size: 97 228 62 949 432 747 268 1206
Fragments arranged by size:
1206 949 747 432 268 228 97 62
EcoO109I rG'GnC_Cy
Cuts at: 696 930 1128 696
Size: 234 198 3557
Fragments arranged by size:
3557 234 198
EcoRI G'AATT_C
Cuts at: 993 993
Size: 3989
EcoRV GAT'ATC
Cuts at: 966 966
Size: 3989
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2008 2008
Size: 3989
FspI TGC'GCA
Cuts at: 523 1770 523
Size: 1247 2742
HaeII r_GCGC'y
Cuts at: 1423 1671 2693 3672 1423
Size: 248 1022 979 1740
Fragments arranged by size:
1740 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 571 603 694 726 784 816 861 893 1831
Size: 32 91 32 58 32 45 32 938
Cuts at: 1831 1861 1863 1893 571
Size: 30 2 30 2667
Fragments arranged by size:
2667 938 91 58 45 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1028 1028
Size: 3989
KasI G'GCGC_C
Cuts at: 1419 1667 1419
Size: 248 3741
KpnI G_GTAC'C
Cuts at: 1009 1009
Size: 3989
MluI A'CGCG_T
Cuts at: 3422 3422
Size: 3989
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2660 2844 2660
Size: 184 3805
MscI TGG'CCA
Cuts at: 1750 1750
Size: 3989
MslI CAynn'nnrTG
Cuts at: 1978 2105 1978
Size: 127 3862
MspA1I CmG'CkG
Cuts at: 550 1100 1414 1774 2787 3032 550
Size: 550 314 360 1013 245 1507
Fragments arranged by size:
1507 1013 550 360 314 245
NaeI GCC'GGC
Cuts at: 2170 2170
Size: 3989
NarI GG'CG_CC
Cuts at: 1420 1668 1420
Size: 248 3741
NdeI CA'TA_TG
Cuts at: 333 333
Size: 3989
NgoMIV G'CCGG_C
Cuts at: 2168 2168
Size: 3989
NspI r_CATG'y
Cuts at: 1042 1542 2449 1042
Size: 500 907 2582
Fragments arranged by size:
2582 907 500
PciI A'CATG_T
Cuts at: 2445 2445
Size: 3989
PfoI T'CCnGG_A
Cuts at: 591 591
Size: 3989
PmeI GTTT'AAAC
Cuts at: 988 988
Size: 3989
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3128 3160 3128
Size: 32 3957
PpuMI rG'GwC_Cy
Cuts at: 696 930 696
Size: 234 3755
PstI C_TGCA'G
Cuts at: 503 775 1036 1721 503
Size: 272 261 685 2771
Fragments arranged by size:
2771 685 272 261
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3989
PvuII CAG'CTG
Cuts at: 550 1414 1774 550
Size: 864 360 2765
Fragments arranged by size:
2765 864 360
RsrII CG'GwC_CG
Cuts at: 2184 2184
Size: 3989
SacI G_AGCT'C
Cuts at: 1003 1003
Size: 3989
SalI G'TCGA_C
Cuts at: 1026 1026
Size: 3989
SapI GCTCTTCn'nnn_
Cuts at: 2012 2222 2012
Size: 210 3779
SbfI CC_TGCA'GG
Cuts at: 1036 1036
Size: 3989
ScaI AGT'ACT
Cuts at: 3506 3506
Size: 3989
SfcI C'TryA_G
Cuts at: 33 499 771 1032 1717 2710 2901 33
Size: 466 272 261 685 993 191 1121
Fragments arranged by size:
1121 993 685 466 272 261 191
SfoI GGC'GCC
Cuts at: 1421 1669 1421
Size: 248 3741
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3989
SmaI CCC'GGG
Cuts at: 223 340 1011 223
Size: 117 671 3201
Fragments arranged by size:
3201 671 117
SmlI C'TyrA_G
Cuts at: 1144 2059 2551 2813 1144
Size: 915 492 262 2320
Fragments arranged by size:
2320 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3989
SphI G_CATG'C
Cuts at: 1042 1542 1042
Size: 500 3489
SrfI GCCC'GGGC
Cuts at: 223 223
Size: 3989
StyI C'CwwG_G
Cuts at: 809 1123 1477 809
Size: 314 354 3321
Fragments arranged by size:
3321 354 314
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1572 1705 2164 2373 1572
Size: 133 459 209 3188
Fragments arranged by size:
3188 459 209 133
TatI w'GTAC_w
Cuts at: 3504 3504
Size: 3989
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3506 3538 3506
Size: 32 3957
Tth111I GACn'n_nGTC
Cuts at: 796 1786 796
Size: 990 2999
XbaI T'CTAG_A
Cuts at: 1020 1020
Size: 3989
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 1484 114
Size: 1370 2619
XmaI C'CCGG_G
Cuts at: 221 338 1009 221
Size: 117 671 3201
Fragments arranged by size:
3201 671 117
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI Bsu36I BtgZI BtsI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NdeI NgoMIV
NspI PciI PfoI PmeI PpiI PpuMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SrfI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI BsaXI BsgI BsiWI BsmI BsrGI BssHII BstEII
BstXI BstZ17I BtgI ClaI DraIII EcoNI FseI FspAI
HindIII HpaI MfeI NcoI NheI NotI NruI NsiI
PacI PasI PflMI PmlI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SspI
StuI SwaI XhoI XmnI ZraI