Restriction map for
pF1KE0159
(Circular) (Six-base) MAPSORT of: pF1KE0159.seq Check: 8912 from: 1 to: 3845
pF1KE0159 3845 bp
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AccI GT'mk_AC
Cuts at: 883 883
Size: 3845
Acc65I G'GTAC_C
Cuts at: 861 861
Size: 3845
AclI AA'CG_TT
Cuts at: 3685 3685
Size: 3845
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 687 1670 2102 2849 3117 687
Size: 983 432 747 268 1415
Fragments arranged by size:
1415 983 747 432 268
AfeI AGC'GCT
Cuts at: 255 255
Size: 3845
AflIII A'CryG_T
Cuts at: 1197 2301 3278 1197
Size: 1104 977 1764
Fragments arranged by size:
1764 1104 977
AgeI A'CCGG_T
Cuts at: 2233 2233
Size: 3845
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 375 375
Size: 3845
AlwNI CAG_nnn'CTG
Cuts at: 597 710 1150 2717 597
Size: 113 440 1567 1725
Fragments arranged by size:
1725 1567 440 113
ApaI G_GGCC'C
Cuts at: 204 204
Size: 3845
ApaLI G'TGCA_C
Cuts at: 2615 2615
Size: 3845
ApoI r'AATT_y
Cuts at: 724 849 3198 3454 724
Size: 125 2349 256 1115
Fragments arranged by size:
2349 1115 256 125
AscI GG'CGCG_CC
Cuts at: 136 136
Size: 3845
AseI AT'TA_AT
Cuts at: 20 20
Size: 3845
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3845
AvaI C'yCGr_G
Cuts at: 625 865 625
Size: 240 3605
BamHI G'GATC_C
Cuts at: 870 870
Size: 3845
BanI G'GyrC_C
Cuts at: 861 1275 1523 1558 861
Size: 414 248 35 3148
Fragments arranged by size:
3148 414 248 35
BanII G_rGCy'C
Cuts at: 204 778 859 1889 204
Size: 574 81 1030 2160
Fragments arranged by size:
2160 1030 574 81
BbeI G_GCGC'C
Cuts at: 1279 1527 1279
Size: 248 3597
BbvCI CC'TCA_GC
Cuts at: 314 314
Size: 3845
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 178 212 178
Size: 34 3811
BciVI GTATCCnnnnn_n'
Cuts at: 657 1743 2504 3845 657
Size: 1086 761 1341 657
Fragments arranged by size:
1341 1086 761 657
BclI T'GATC_A
Cuts at: 1365 1365
Size: 3845
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 3845
BglII A'GATC_T
Cuts at: 1360 3397 1360
Size: 2037 1808
BlpI GC'TnA_GC
Cuts at: 957 957
Size: 3845
Bme1580I G_kGCm'C
Cuts at: 204 1470 1563 2619 204
Size: 1266 93 1056 1430
Fragments arranged by size:
1430 1266 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1057 1138 1473 3617 1057
Size: 81 335 2144 1285
Fragments arranged by size:
2144 1285 335 81
BmtI G_CTAG'C
Cuts at: 260 260
Size: 3845
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2212 2212
Size: 3845
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 389 444 389
Size: 55 3790
Bpu10I CC'TnA_GC
Cuts at: 314 777 314
Size: 463 3382
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 469 1021 1900 2392 2690 469
Size: 552 879 492 298 1624
Fragments arranged by size:
1624 879 552 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3549 3549
Size: 3845
BsaAI yAC'GTr
Cuts at: 1198 1198
Size: 3845
BsaBI GATnn'nnATC
Cuts at: 6 1359 3396 6
Size: 1353 2037 455
Fragments arranged by size:
2037 1353 455
BsaHI Gr'CG_yC
Cuts at: 1276 1524 2226 3722 1276
Size: 248 702 1496 1399
Fragments arranged by size:
1496 1399 702 248
BsaWI w'CCGG_w
Cuts at: 1034 1555 2233 2507 2654 3065 1034
Size: 521 678 274 147 411 1814
Fragments arranged by size:
1814 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 691 721 691
Size: 30 3815
BseYI C'CCAG_C
Cuts at: 601 828 1270 2028 2605 3101 601
Size: 227 442 758 577 496 1345
Fragments arranged by size:
1345 758 577 496 442 227
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 208 208
Size: 3845
BsiEI CG_ry'CG
Cuts at: 104 772 1387 1433 2641 104
Size: 668 615 46 1208 1308
Fragments arranged by size:
1308 1208 668 615 46
BsiHKAI G_wGCw'C
Cuts at: 650 778 859 1637 1827 2619 650
Size: 128 81 778 190 792 1876
Fragments arranged by size:
1876 792 778 190 128 81
Bsp1286I G_dGCh'C
Cuts at: 204 650 778 859 1470 1563 1637 1827 1889
Size: 446 128 81 611 93 74 190 62
Cuts at: 1889 2619 204
Size: 730 1430
Fragments arranged by size:
1430 730 611 446 190 128 93 81
74 62
BspEI T'CCGG_A
Cuts at: 3065 3065
Size: 3845
BspHI T'CATG_A
Cuts at: 3021 3021
Size: 3845
BspMI ACCTGCnnnn'nnnn_
Cuts at: 895 1109 1411 1792 895
Size: 214 302 381 2948
Fragments arranged by size:
2948 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2137 2191 3675 3840 50
Size: 2087 54 1484 165 55
Fragments arranged by size:
2087 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 393 1757 393
Size: 1364 2481
BsrFI r'CCGG_y
Cuts at: 1843 2024 2233 1843
Size: 181 209 3455
Fragments arranged by size:
3455 209 181
BssHII G'CGCG_C
Cuts at: 136 136
Size: 3845
BssSI C'ACGA_G
Cuts at: 2116 2474 2116
Size: 358 3487
BstAPI GCAn_nnn'nTGC
Cuts at: 523 1222 523
Size: 699 3146
BstBI TT'CG_AA
Cuts at: 2206 2206
Size: 3845
BstEII G'GTnAC_C
Cuts at: 654 798 654
Size: 144 3701
BstXI CCAn_nnnn'nTGG
Cuts at: 468 468
Size: 3845
BstYI r'GATC_y
Cuts at: 1 870 1337 1360 1695 1941 2953 3039 3051
Size: 869 467 23 335 246 1012 86 12
Cuts at: 3051 3397 1
Size: 346 449
Fragments arranged by size:
1012 869 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 692 692
Size: 3845
BtgI C'CryG_G
Cuts at: 107 107
Size: 3845
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 624 1795 1976 2139 624
Size: 1171 181 163 2330
Fragments arranged by size:
2330 1171 181 163
BtsI GCAGTG_nn'
Cuts at: 1088 1088
Size: 3845
DraI TTT'AAA
Cuts at: 844 844
Size: 3845
DrdI GACnn_nn'nnGTC
Cuts at: 1551 2409 1551
Size: 858 2987
EaeI y'GGCC_r
Cuts at: 563 1430 1604 1995 2022 3367 563
Size: 867 174 391 27 1345 1041
Fragments arranged by size:
1345 1041 867 391 174 27
EagI C'GGCC_G
Cuts at: 1430 1430
Size: 3845
EarI CTCTTCn'nnn_
Cuts at: 239 332 1868 2078 239
Size: 93 1536 210 2006
Fragments arranged by size:
2006 1536 210 93
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2053 2363 2509 3655 2053
Size: 310 146 1146 2243
Fragments arranged by size:
2243 1146 310 146
EcoICRI GAG'CTC
Cuts at: 776 857 776
Size: 81 3764
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 389 444 687 1670 2102 2849 3117 389
Size: 55 243 983 432 747 268 1117
Fragments arranged by size:
1117 983 747 432 268 243 55
EcoNI CCTnn'n_nnAGG
Cuts at: 790 790
Size: 3845
EcoO109I rG'GnC_Cy
Cuts at: 984 984
Size: 3845
EcoRI G'AATT_C
Cuts at: 724 849 724
Size: 125 3720
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 423 1864 423
Size: 1441 2404
FspI TGC'GCA
Cuts at: 1626 1626
Size: 3845
HaeII r_GCGC'y
Cuts at: 170 257 1279 1527 2549 3528 170
Size: 87 1022 248 1022 979 487
Fragments arranged by size:
1022 1022 979 487 248 87
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 691 723 1687 1717 1719 1749 691
Size: 32 964 30 2 30 2787
Fragments arranged by size:
2787 964 32 30 30 2
HincII GTy'rAC
Cuts at: 884 884
Size: 3845
KasI G'GCGC_C
Cuts at: 1275 1523 1275
Size: 248 3597
KpnI G_GTAC'C
Cuts at: 865 865
Size: 3845
MluI A'CGCG_T
Cuts at: 3278 3278
Size: 3845
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 300 2516 2700 300
Size: 2216 184 1445
Fragments arranged by size:
2216 1445 184
MscI TGG'CCA
Cuts at: 565 1606 565
Size: 1041 2804
MslI CAynn'nnrTG
Cuts at: 643 1834 1961 643
Size: 1191 127 2527
Fragments arranged by size:
2527 1191 127
MspA1I CmG'CkG
Cuts at: 614 956 1270 1630 2643 2888 614
Size: 342 314 360 1013 245 1571
Fragments arranged by size:
1571 1013 360 342 314 245
NaeI GCC'GGC
Cuts at: 2026 2026
Size: 3845
NarI GG'CG_CC
Cuts at: 1276 1524 1276
Size: 248 3597
NcoI C'CATG_G
Cuts at: 107 107
Size: 3845
NdeI CA'TA_TG
Cuts at: 442 442
Size: 3845
NgoMIV G'CCGG_C
Cuts at: 2024 2024
Size: 3845
NheI G'CTAG_C
Cuts at: 256 256
Size: 3845
NspI r_CATG'y
Cuts at: 621 898 1398 2305 621
Size: 277 500 907 2161
Fragments arranged by size:
2161 907 500 277
PciI A'CATG_T
Cuts at: 2301 2301
Size: 3845
PflMI CCAn_nnn'nTGG
Cuts at: 597 597
Size: 3845
PfoI T'CCnGG_A
Cuts at: 709 709
Size: 3845
PmeI GTTT'AAAC
Cuts at: 844 844
Size: 3845
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2984 3016 2984
Size: 32 3813
PspOMI G'GGCC_C
Cuts at: 200 200
Size: 3845
PstI C_TGCA'G
Cuts at: 129 892 1577 129
Size: 763 685 2397
Fragments arranged by size:
2397 763 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3845
PvuII CAG'CTG
Cuts at: 1270 1630 1270
Size: 360 3485
RsrII CG'GwC_CG
Cuts at: 2040 2040
Size: 3845
SacI G_AGCT'C
Cuts at: 778 859 778
Size: 81 3764
SalI G'TCGA_C
Cuts at: 882 882
Size: 3845
SapI GCTCTTCn'nnn_
Cuts at: 1868 2078 1868
Size: 210 3635
SbfI CC_TGCA'GG
Cuts at: 892 892
Size: 3845
ScaI AGT'ACT
Cuts at: 3362 3362
Size: 3845
SexAI A'CCwGG_T
Cuts at: 302 302
Size: 3845
SfcI C'TryA_G
Cuts at: 33 125 888 1573 2566 2757 33
Size: 92 763 685 993 191 1121
Fragments arranged by size:
1121 993 763 685 191 92
SfoI GGC'GCC
Cuts at: 1277 1525 1277
Size: 248 3597
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3845
SmaI CCC'GGG
Cuts at: 627 867 627
Size: 240 3605
SmlI C'TyrA_G
Cuts at: 484 1000 1915 2407 2669 484
Size: 516 915 492 262 1660
Fragments arranged by size:
1660 915 516 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3845
SphI G_CATG'C
Cuts at: 621 898 1398 621
Size: 277 500 3068
Fragments arranged by size:
3068 500 277
StuI AGG'CCT
Cuts at: 497 497
Size: 3845
StyI C'CwwG_G
Cuts at: 107 506 566 979 1333 107
Size: 399 60 413 354 2619
Fragments arranged by size:
2619 413 399 354 60
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 322 786 1428 1561 2020 2229 322
Size: 464 642 133 459 209 1938
Fragments arranged by size:
1938 642 464 459 209 133
TatI w'GTAC_w
Cuts at: 3360 3360
Size: 3845
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3362 3394 3362
Size: 32 3813
Tth111I GACn'n_nGTC
Cuts at: 1642 1642
Size: 3845
XbaI T'CTAG_A
Cuts at: 876 876
Size: 3845
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 228 598 1340 228
Size: 370 742 2733
Fragments arranged by size:
2733 742 370
XmaI C'CCGG_G
Cuts at: 625 865 625
Size: 240 3605
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NspI PciI PflMI PfoI PmeI
PpiI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AloI AvrII BaeI
BbsI BfrBI BmgBI BseRI BsiWI BsmI BsmBI BsrGI
BstZ17I ClaI CspCI DraIII EcoRV FseI FspAI HindIII
HpaI MfeI NotI NruI NsiI PacI PasI PmlI
PpuMI PshAI PsiI PspXI PsrI SacII SanDI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI