FASTA searches a protein or DNA sequence data bank 36.3.4 Apr, 2011 Please cite: W.R. Pearson & D.J. Lipman PNAS (1988) 85:2444-2448 Query: pF1KE0162, 310 aa 1>>>pF1KE0162 310 - 310 aa - 310 aa Library: human.CCDS.faa 18511270 residues in 32554 sequences Statistics: Expectation_n fit: rho(ln(x))= 5.2184+/-0.000695; mu= 16.4810+/- 0.042 mean_var=68.4919+/-13.436, 0's: 0 Z-trim(110.1): 18 B-trim: 3 in 1/51 Lambda= 0.154973 statistics sampled from 11323 (11341) to 11323 sequences Algorithm: FASTA (3.7 Nov 2010) [optimized] Parameters: BL50 matrix (15:-5), open/ext: -10/-2 ktup: 2, E-join: 1 (0.722), E-opt: 0.2 (0.348), width: 16 Scan time: 2.420 The best scores are: opt bits E(32554) CCDS4526.1 SIRT5 gene_id:23408|Hs108|chr6 ( 310) 2134 485.6 2e-137 CCDS4527.1 SIRT5 gene_id:23408|Hs108|chr6 ( 299) 1952 444.9 3.5e-125 CCDS56398.1 SIRT5 gene_id:23408|Hs108|chr6 ( 202) 1387 318.5 2.7e-87 CCDS54966.1 SIRT5 gene_id:23408|Hs108|chr6 ( 292) 1295 298.0 5.6e-81 CCDS9194.1 SIRT4 gene_id:23409|Hs108|chr12 ( 314) 396 97.1 1.9e-20 >>CCDS4526.1 SIRT5 gene_id:23408|Hs108|chr6 (310 aa) initn: 2134 init1: 2134 opt: 2134 Z-score: 2580.7 bits: 485.6 E(32554): 2e-137 Smith-Waterman score: 2134; 100.0% identity (100.0% similar) in 310 aa overlap (1-310:1-310) 10 20 30 40 50 60 pF1KE0 MRPLQIVPSRLISQLYCGLKPPASTRNQICLKMARPSSSMADFRKFFAKAKHIVIISGAG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: CCDS45 MRPLQIVPSRLISQLYCGLKPPASTRNQICLKMARPSSSMADFRKFFAKAKHIVIISGAG 10 20 30 40 50 60 70 80 90 100 110 120 pF1KE0 VSAESGVPTFRGAGGYWRKWQAQDLATPLAFAHNPSRVWEFYHYRREVMGSKEPNAGHRA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: CCDS45 VSAESGVPTFRGAGGYWRKWQAQDLATPLAFAHNPSRVWEFYHYRREVMGSKEPNAGHRA 70 80 90 100 110 120 130 140 150 160 170 180 pF1KE0 IAECETRLGKQGRRVVVITQNIDELHRKAGTKNLLEIHGSLFKTRCTSCGVVAENYKSPI :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: CCDS45 IAECETRLGKQGRRVVVITQNIDELHRKAGTKNLLEIHGSLFKTRCTSCGVVAENYKSPI 130 140 150 160 170 180 190 200 210 220 230 240 pF1KE0 CPALSGKGAPEPGTQDASIPVEKLPRCEEAGCGGLLRPHVVWFGENLDPAILEEVDRELA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: CCDS45 CPALSGKGAPEPGTQDASIPVEKLPRCEEAGCGGLLRPHVVWFGENLDPAILEEVDRELA 190 200 210 220 230 240 250 260 270 280 290 300 pF1KE0 HCDLCLVVGTSSVVYPAAMFAPQVAARGVPVAEFNTETTPATNRFRFHFQGPCGTTLPEA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: CCDS45 HCDLCLVVGTSSVVYPAAMFAPQVAARGVPVAEFNTETTPATNRFRFHFQGPCGTTLPEA 250 260 270 280 290 300 310 pF1KE0 LACHENETVS :::::::::: CCDS45 LACHENETVS 310 >>CCDS4527.1 SIRT5 gene_id:23408|Hs108|chr6 (299 aa) initn: 1952 init1: 1952 opt: 1952 Z-score: 2361.0 bits: 444.9 E(32554): 3.5e-125 Smith-Waterman score: 1952; 100.0% identity (100.0% similar) in 285 aa overlap (1-285:1-285) 10 20 30 40 50 60 pF1KE0 MRPLQIVPSRLISQLYCGLKPPASTRNQICLKMARPSSSMADFRKFFAKAKHIVIISGAG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: CCDS45 MRPLQIVPSRLISQLYCGLKPPASTRNQICLKMARPSSSMADFRKFFAKAKHIVIISGAG 10 20 30 40 50 60 70 80 90 100 110 120 pF1KE0 VSAESGVPTFRGAGGYWRKWQAQDLATPLAFAHNPSRVWEFYHYRREVMGSKEPNAGHRA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: CCDS45 VSAESGVPTFRGAGGYWRKWQAQDLATPLAFAHNPSRVWEFYHYRREVMGSKEPNAGHRA 70 80 90 100 110 120 130 140 150 160 170 180 pF1KE0 IAECETRLGKQGRRVVVITQNIDELHRKAGTKNLLEIHGSLFKTRCTSCGVVAENYKSPI :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: CCDS45 IAECETRLGKQGRRVVVITQNIDELHRKAGTKNLLEIHGSLFKTRCTSCGVVAENYKSPI 130 140 150 160 170 180 190 200 210 220 230 240 pF1KE0 CPALSGKGAPEPGTQDASIPVEKLPRCEEAGCGGLLRPHVVWFGENLDPAILEEVDRELA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: CCDS45 CPALSGKGAPEPGTQDASIPVEKLPRCEEAGCGGLLRPHVVWFGENLDPAILEEVDRELA 190 200 210 220 230 240 250 260 270 280 290 300 pF1KE0 HCDLCLVVGTSSVVYPAAMFAPQVAARGVPVAEFNTETTPATNRFRFHFQGPCGTTLPEA ::::::::::::::::::::::::::::::::::::::::::::: CCDS45 HCDLCLVVGTSSVVYPAAMFAPQVAARGVPVAEFNTETTPATNRFSHLISISSLIIIKN 250 260 270 280 290 310 pF1KE0 LACHENETVS >>CCDS56398.1 SIRT5 gene_id:23408|Hs108|chr6 (202 aa) initn: 1387 init1: 1387 opt: 1387 Z-score: 1680.8 bits: 318.5 E(32554): 2.7e-87 Smith-Waterman score: 1387; 100.0% identity (100.0% similar) in 202 aa overlap (109-310:1-202) 80 90 100 110 120 130 pF1KE0 KWQAQDLATPLAFAHNPSRVWEFYHYRREVMGSKEPNAGHRAIAECETRLGKQGRRVVVI :::::::::::::::::::::::::::::: CCDS56 MGSKEPNAGHRAIAECETRLGKQGRRVVVI 10 20 30 140 150 160 170 180 190 pF1KE0 TQNIDELHRKAGTKNLLEIHGSLFKTRCTSCGVVAENYKSPICPALSGKGAPEPGTQDAS :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: CCDS56 TQNIDELHRKAGTKNLLEIHGSLFKTRCTSCGVVAENYKSPICPALSGKGAPEPGTQDAS 40 50 60 70 80 90 200 210 220 230 240 250 pF1KE0 IPVEKLPRCEEAGCGGLLRPHVVWFGENLDPAILEEVDRELAHCDLCLVVGTSSVVYPAA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: CCDS56 IPVEKLPRCEEAGCGGLLRPHVVWFGENLDPAILEEVDRELAHCDLCLVVGTSSVVYPAA 100 110 120 130 140 150 260 270 280 290 300 310 pF1KE0 MFAPQVAARGVPVAEFNTETTPATNRFRFHFQGPCGTTLPEALACHENETVS :::::::::::::::::::::::::::::::::::::::::::::::::::: CCDS56 MFAPQVAARGVPVAEFNTETTPATNRFRFHFQGPCGTTLPEALACHENETVS 160 170 180 190 200 >>CCDS54966.1 SIRT5 gene_id:23408|Hs108|chr6 (292 aa) initn: 1295 init1: 1295 opt: 1295 Z-score: 1567.3 bits: 298.0 E(32554): 5.6e-81 Smith-Waterman score: 1965; 94.2% identity (94.2% similar) in 310 aa overlap (1-310:1-292) 10 20 30 40 50 60 pF1KE0 MRPLQIVPSRLISQLYCGLKPPASTRNQICLKMARPSSSMADFRKFFAKAKHIVIISGAG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: CCDS54 MRPLQIVPSRLISQLYCGLKPPASTRNQICLKMARPSSSMADFRKFFAKAKHIVIISGAG 10 20 30 40 50 60 70 80 90 100 110 120 pF1KE0 VSAESGVPTFRGAGGYWRKWQAQDLATPLAFAHNPSRVWEFYHYRREVMGSKEPNAGHRA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: CCDS54 VSAESGVPTFRGAGGYWRKWQAQDLATPLAFAHNPSRVWEFYHYRREVMGSKEPNAGHRA 70 80 90 100 110 120 130 140 150 160 170 180 pF1KE0 IAECETRLGKQGRRVVVITQNIDELHRKAGTKNLLEIHGSLFKTRCTSCGVVAENYKSPI :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: CCDS54 IAECETRLGKQGRRVVVITQNIDELHRKAGTKNLLEIHGSLFKTRCTSCGVVAENYKSPI 130 140 150 160 170 180 190 200 210 220 230 240 pF1KE0 CPALSGKGAPEPGTQDASIPVEKLPRCEEAGCGGLLRPHVVWFGENLDPAILEEVDRELA :::::::: :::::::::::::::::::::::::::::::::: CCDS54 CPALSGKG------------------CEEAGCGGLLRPHVVWFGENLDPAILEEVDRELA 190 200 210 220 250 260 270 280 290 300 pF1KE0 HCDLCLVVGTSSVVYPAAMFAPQVAARGVPVAEFNTETTPATNRFRFHFQGPCGTTLPEA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: CCDS54 HCDLCLVVGTSSVVYPAAMFAPQVAARGVPVAEFNTETTPATNRFRFHFQGPCGTTLPEA 230 240 250 260 270 280 310 pF1KE0 LACHENETVS :::::::::: CCDS54 LACHENETVS 290 >>CCDS9194.1 SIRT4 gene_id:23409|Hs108|chr12 (314 aa) initn: 366 init1: 166 opt: 396 Z-score: 480.5 bits: 97.1 E(32554): 1.9e-20 Smith-Waterman score: 396; 27.2% identity (55.0% similar) in 309 aa overlap (8-303:20-314) 10 20 30 40 pF1KE0 MRPLQIVPSRLISQLYCGLKPPASTRNQICLKMARPSSSMADFRKFFA ::. :. :: ::: .. ....:.. CCDS91 MKMSFALTFRSAKGRWIANPSQPCSKASIGLFVPASP--------PLDPEKVKELQRFIT 10 20 30 40 50 50 60 70 80 90 100 pF1KE0 KAKHIVIISGAGVSAESGVPTFRGAG-GYWRKWQAQDLATPLAFAHNP--SRVWEFYHYR .:......:::.:.:::.: .:. : . . . . . : .: : CCDS91 LSKRLLVMTGAGISTESGIPDYRSEKVGLYARTDRRPIQHGDFVRSAPIRQRYWARNFVG 60 70 80 90 100 110 110 120 130 140 150 160 pF1KE0 REVMGSKEPNAGHRAIAECETRLGKQGRRVVVITQNIDELHRKAGTKNLLEIHGSLFKTR ..:..:: .: :.. : : :. ..:::.: :: :::.. : :.:: . .. CCDS91 WPQFSSHQPNPAHWALSTWE----KLGKLYWLVTQNVDALHTKAGSRRLTELHGCMDRVL 120 130 140 150 160 170 180 190 200 210 pF1KE0 CTSCG------VVAENYK--SPICPALSGKGAPEPGT--QDASIPVEKLPRCEEAGCGGL : .:: :. : .. .: : . ::. . .. .. ..: : . ::: CCDS91 CLDCGEQTPRGVLQERFQVLNPTWSAEAHGLAPDGDVFLSEEQVRSFQVPTCVQ--CGGH 170 180 190 200 210 220 220 230 240 250 260 270 pF1KE0 LRPHVVWFGENLDPAILEEVDRELAHCDLCLVVGTSSVVYPAAMFAPQVAARGVPVAEFN :.: ::.::....: .. : ... . : ::::.: :: . : . . .:.: .: CCDS91 LKPDVVFFGDTVNPDKVDFVHKRVKEADSLLVVGSSLQVYSGYRFILTAWEKKLPIAILN 230 240 250 260 270 280 280 290 300 310 pF1KE0 TETTPATNRFRFHFQGPCGTTLPEALACHENETVS : . . ..... :: :: : CCDS91 IGPTRSDDLACLKLNSRCGELLPLIDPC 290 300 310 310 residues in 1 query sequences 18511270 residues in 32554 library sequences Tcomplib [36.3.4 Apr, 2011] (8 proc) start: Thu Nov 3 23:22:03 2016 done: Thu Nov 3 23:22:04 2016 Total Scan time: 2.420 Total Display time: 0.000 Function used was FASTA [36.3.4 Apr, 2011]