Restriction map for
pF1KE0166
(Circular) (Six-base) MAPSORT of: pF1KE0166.seq Check: 9044 from: 1 to: 3857
pF1KE0166 3857 bp
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AccI GT'mk_AC
Cuts at: 895 895
Size: 3857
Acc65I G'GTAC_C
Cuts at: 873 873
Size: 3857
AclI AA'CG_TT
Cuts at: 3697 3697
Size: 3857
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1682 2114 2861 3129 1682
Size: 432 747 268 2410
Fragments arranged by size:
2410 747 432 268
AfeI AGC'GCT
Cuts at: 183 777 183
Size: 594 3263
AflIII A'CryG_T
Cuts at: 1209 2313 3290 1209
Size: 1104 977 1776
Fragments arranged by size:
1776 1104 977
AgeI A'CCGG_T
Cuts at: 2245 2245
Size: 3857
AhdI GACnn_n'nnGTC
Cuts at: 629 629
Size: 3857
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 523 523
Size: 3857
AlwNI CAG_nnn'CTG
Cuts at: 1162 2729 1162
Size: 1567 2290
ApaLI G'TGCA_C
Cuts at: 2627 2627
Size: 3857
ApoI r'AATT_y
Cuts at: 861 3210 3466 861
Size: 2349 256 1252
Fragments arranged by size:
2349 1252 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3857
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3857
AvaI C'yCGr_G
Cuts at: 877 877
Size: 3857
BamHI G'GATC_C
Cuts at: 882 882
Size: 3857
BanI G'GyrC_C
Cuts at: 873 1287 1535 1570 873
Size: 414 248 35 3160
Fragments arranged by size:
3160 414 248 35
BanII G_rGCy'C
Cuts at: 203 871 1901 203
Size: 668 1030 2159
Fragments arranged by size:
2159 1030 668
BbeI G_GCGC'C
Cuts at: 1291 1539 1291
Size: 248 3609
BciVI GTATCCnnnnn_n'
Cuts at: 1755 2516 3857 1755
Size: 761 1341 1755
Fragments arranged by size:
1755 1341 761
BclI T'GATC_A
Cuts at: 1377 1377
Size: 3857
BglII A'GATC_T
Cuts at: 1372 3409 1372
Size: 2037 1820
BlpI GC'TnA_GC
Cuts at: 969 969
Size: 3857
Bme1580I G_kGCm'C
Cuts at: 1482 1575 2631 1482
Size: 93 1056 2708
Fragments arranged by size:
2708 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 521 578 1069 1150 1485 3629 521
Size: 57 491 81 335 2144 749
Fragments arranged by size:
2144 749 491 335 81 57
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2224 2224
Size: 3857
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 428 437 659 776 428
Size: 9 222 117 3509
Fragments arranged by size:
3509 222 117 9
Bpu10I CC'TnA_GC
Cuts at: 113 113
Size: 3857
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 157 396 1033 1912 2404 2702 157
Size: 239 637 879 492 298 1312
Fragments arranged by size:
1312 879 637 492 298 239
BsaI GGTCTCn'nnnn_
Cuts at: 3561 3561
Size: 3857
BsaAI yAC'GTr
Cuts at: 1210 1210
Size: 3857
BsaBI GATnn'nnATC
Cuts at: 6 1371 3408 6
Size: 1365 2037 455
Fragments arranged by size:
2037 1365 455
BsaHI Gr'CG_yC
Cuts at: 1288 1536 2238 3734 1288
Size: 248 702 1496 1411
Fragments arranged by size:
1496 1411 702 248
BsaWI w'CCGG_w
Cuts at: 1046 1567 2245 2519 2666 3077 1046
Size: 521 678 274 147 411 1826
Fragments arranged by size:
1826 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 240 270 240
Size: 30 3827
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 183 183
Size: 3857
BseYI C'CCAG_C
Cuts at: 1282 2040 2617 3113 1282
Size: 758 577 496 2026
Fragments arranged by size:
2026 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 302 302
Size: 3857
BsiEI CG_ry'CG
Cuts at: 104 1399 1445 2653 104
Size: 1295 46 1208 1308
Fragments arranged by size:
1308 1295 1208 46
BsiHKAI G_wGCw'C
Cuts at: 871 1649 1839 2631 871
Size: 778 190 792 2097
Fragments arranged by size:
2097 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 203 871 1482 1575 1649 1839 1901 2631 203
Size: 668 611 93 74 190 62 730 1429
Fragments arranged by size:
1429 730 668 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3077 3077
Size: 3857
BspHI T'CATG_A
Cuts at: 3033 3033
Size: 3857
BspMI ACCTGCnnnn'nnnn_
Cuts at: 907 1121 1423 1804 907
Size: 214 302 381 2960
Fragments arranged by size:
2960 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2149 2203 3687 3852 50
Size: 2099 54 1484 165 55
Fragments arranged by size:
2099 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 350 1769 350
Size: 1419 2438
BsrFI r'CCGG_y
Cuts at: 1855 2036 2245 1855
Size: 181 209 3467
Fragments arranged by size:
3467 209 181
BssSI C'ACGA_G
Cuts at: 2128 2486 2128
Size: 358 3499
BstAPI GCAn_nnn'nTGC
Cuts at: 1234 1234
Size: 3857
BstBI TT'CG_AA
Cuts at: 2218 2218
Size: 3857
BstYI r'GATC_y
Cuts at: 1 172 882 1349 1372 1707 1953 2965 3051
Size: 171 710 467 23 335 246 1012 86
Cuts at: 3051 3063 3409 1
Size: 12 346 449
Fragments arranged by size:
1012 710 467 449 346 335 246 171
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 166 166
Size: 3857
BtgI C'CryG_G
Cuts at: 107 195 107
Size: 88 3769
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1807 1988 2151 1807
Size: 181 163 3513
Fragments arranged by size:
3513 181 163
BtsI GCAGTG_nn'
Cuts at: 507 747 1100 507
Size: 240 353 3264
Fragments arranged by size:
3264 353 240
DraI TTT'AAA
Cuts at: 856 856
Size: 3857
DrdI GACnn_nn'nnGTC
Cuts at: 1563 2421 1563
Size: 858 2999
EaeI y'GGCC_r
Cuts at: 1442 1616 2007 2034 3379 1442
Size: 174 391 27 1345 1920
Fragments arranged by size:
1920 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1442 1442
Size: 3857
EarI CTCTTCn'nnn_
Cuts at: 652 822 1880 2090 652
Size: 170 1058 210 2419
Fragments arranged by size:
2419 1058 210 170
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2065 2375 2521 3667 2065
Size: 310 146 1146 2255
Fragments arranged by size:
2255 1146 310 146
EcoICRI GAG'CTC
Cuts at: 869 869
Size: 3857
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 428 437 659 776 1682 2114 2861 3129 428
Size: 9 222 117 906 432 747 268 1156
Fragments arranged by size:
1156 906 747 432 268 222 117 9
EcoNI CCTnn'n_nnAGG
Cuts at: 214 214
Size: 3857
EcoO109I rG'GnC_Cy
Cuts at: 996 996
Size: 3857
EcoRI G'AATT_C
Cuts at: 861 861
Size: 3857
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 663 1876 663
Size: 1213 2644
FspI TGC'GCA
Cuts at: 1638 1638
Size: 3857
HaeII r_GCGC'y
Cuts at: 185 779 1291 1539 2561 3540 185
Size: 594 512 248 1022 979 502
Fragments arranged by size:
1022 979 594 512 502 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 615 647 822 826 854 858 1699 1729 1731
Size: 32 175 4 28 4 841 30 2
Cuts at: 1731 1761 615
Size: 30 2711
Fragments arranged by size:
2711 841 175 32 30 30 28 4
4 2
HincII GTy'rAC
Cuts at: 896 896
Size: 3857
KasI G'GCGC_C
Cuts at: 1287 1535 1287
Size: 248 3609
KpnI G_GTAC'C
Cuts at: 877 877
Size: 3857
MluI A'CGCG_T
Cuts at: 3290 3290
Size: 3857
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 249 2528 2712 249
Size: 2279 184 1394
Fragments arranged by size:
2279 1394 184
MscI TGG'CCA
Cuts at: 1618 1618
Size: 3857
MslI CAynn'nnrTG
Cuts at: 829 1846 1973 829
Size: 1017 127 2713
Fragments arranged by size:
2713 1017 127
MspA1I CmG'CkG
Cuts at: 762 968 1282 1642 2655 2900 762
Size: 206 314 360 1013 245 1719
Fragments arranged by size:
1719 1013 360 314 245 206
NaeI GCC'GGC
Cuts at: 2038 2038
Size: 3857
NarI GG'CG_CC
Cuts at: 1288 1536 1288
Size: 248 3609
NcoI C'CATG_G
Cuts at: 107 195 107
Size: 88 3769
NgoMIV G'CCGG_C
Cuts at: 2036 2036
Size: 3857
NspI r_CATG'y
Cuts at: 803 910 1410 2317 803
Size: 107 500 907 2343
Fragments arranged by size:
2343 907 500 107
PciI A'CATG_T
Cuts at: 2313 2313
Size: 3857
PmeI GTTT'AAAC
Cuts at: 856 856
Size: 3857
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2996 3028 2996
Size: 32 3825
PstI C_TGCA'G
Cuts at: 904 1589 904
Size: 685 3172
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3857
PvuII CAG'CTG
Cuts at: 1282 1642 1282
Size: 360 3497
RsrII CG'GwC_CG
Cuts at: 2052 2052
Size: 3857
SacI G_AGCT'C
Cuts at: 871 871
Size: 3857
SalI G'TCGA_C
Cuts at: 894 894
Size: 3857
SapI GCTCTTCn'nnn_
Cuts at: 822 1880 2090 822
Size: 1058 210 2589
Fragments arranged by size:
2589 1058 210
SbfI CC_TGCA'GG
Cuts at: 904 904
Size: 3857
ScaI AGT'ACT
Cuts at: 3374 3374
Size: 3857
SfcI C'TryA_G
Cuts at: 33 900 1585 2578 2769 33
Size: 867 685 993 191 1121
Fragments arranged by size:
1121 993 867 685 191
SfoI GGC'GCC
Cuts at: 1289 1537 1289
Size: 248 3609
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3857
SmaI CCC'GGG
Cuts at: 879 879
Size: 3857
SmlI C'TyrA_G
Cuts at: 136 375 1012 1927 2419 2681 136
Size: 239 637 915 492 262 1312
Fragments arranged by size:
1312 915 637 492 262 239
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3857
SphI G_CATG'C
Cuts at: 803 910 1410 803
Size: 107 500 3250
Fragments arranged by size:
3250 500 107
StuI AGG'CCT
Cuts at: 293 293
Size: 3857
StyI C'CwwG_G
Cuts at: 107 195 651 848 991 1345 107
Size: 88 456 197 143 354 2619
Fragments arranged by size:
2619 456 354 197 143 88
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 480 861 1440 1573 2032 2241 480
Size: 381 579 133 459 209 2096
Fragments arranged by size:
2096 579 459 381 209 133
TatI w'GTAC_w
Cuts at: 256 3372 256
Size: 3116 741
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 206 238 3374 3406 206
Size: 32 3136 32 657
Fragments arranged by size:
3136 657 32 32
Tth111I GACn'n_nGTC
Cuts at: 1654 1654
Size: 3857
XbaI T'CTAG_A
Cuts at: 888 888
Size: 3857
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1352 1352
Size: 3857
XmaI C'CCGG_G
Cuts at: 877 877
Size: 3857
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PmeI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AleI AloI ApaI AscI AvrII
BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI BmtI
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I
ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI XmnI ZraI