Restriction map for
pF1KE0168
(Circular) (Six-base) MAPSORT of: pF1KE0168.seq Check: 1607 from: 1 to: 3512
pF1KE0168 3512 bp
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AccI GT'mk_AC
Cuts at: 550 550
Size: 3512
Acc65I G'GTAC_C
Cuts at: 528 528
Size: 3512
AclI AA'CG_TT
Cuts at: 3352 3352
Size: 3512
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 436 1337 1769 2516 2784 436
Size: 901 432 747 268 1164
Fragments arranged by size:
1164 901 747 432 268
AflIII A'CryG_T
Cuts at: 117 864 1968 2945 117
Size: 747 1104 977 684
Fragments arranged by size:
1104 977 747 684
AgeI A'CCGG_T
Cuts at: 467 1900 467
Size: 1433 2079
AlwNI CAG_nnn'CTG
Cuts at: 817 2384 817
Size: 1567 1945
ApaLI G'TGCA_C
Cuts at: 2282 2282
Size: 3512
ApoI r'AATT_y
Cuts at: 516 2865 3121 516
Size: 2349 256 907
Fragments arranged by size:
2349 907 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3512
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3512
AvaI C'yCGr_G
Cuts at: 532 532
Size: 3512
BamHI G'GATC_C
Cuts at: 537 537
Size: 3512
BanI G'GyrC_C
Cuts at: 230 528 942 1190 1225 230
Size: 298 414 248 35 2517
Fragments arranged by size:
2517 414 298 248 35
BanII G_rGCy'C
Cuts at: 526 1556 526
Size: 1030 2482
BbeI G_GCGC'C
Cuts at: 946 1194 946
Size: 248 3264
BciVI GTATCCnnnnn_n'
Cuts at: 1410 2171 3512 1410
Size: 761 1341 1410
Fragments arranged by size:
1410 1341 761
BclI T'GATC_A
Cuts at: 1032 1032
Size: 3512
BglII A'GATC_T
Cuts at: 1027 3064 1027
Size: 2037 1475
BlpI GC'TnA_GC
Cuts at: 624 624
Size: 3512
Bme1580I G_kGCm'C
Cuts at: 311 1137 1230 2286 311
Size: 826 93 1056 1537
Fragments arranged by size:
1537 1056 826 93
BmrI ACTGGGnnnn_n'
Cuts at: 724 805 1140 3284 724
Size: 81 335 2144 952
Fragments arranged by size:
2144 952 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1879 1879
Size: 3512
Bpu10I CC'TnA_GC
Cuts at: 438 438
Size: 3512
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 346 688 1567 2059 2357 346
Size: 342 879 492 298 1501
Fragments arranged by size:
1501 879 492 342 298
BsaI GGTCTCn'nnnn_
Cuts at: 3216 3216
Size: 3512
BsaAI yAC'GTr
Cuts at: 865 865
Size: 3512
BsaBI GATnn'nnATC
Cuts at: 6 1026 3063 6
Size: 1020 2037 455
Fragments arranged by size:
2037 1020 455
BsaHI Gr'CG_yC
Cuts at: 322 943 1191 1893 3389 322
Size: 621 248 702 1496 445
Fragments arranged by size:
1496 702 621 445 248
BsaWI w'CCGG_w
Cuts at: 467 701 1222 1900 2174 2321 2732 467
Size: 234 521 678 274 147 411 1247
Fragments arranged by size:
1247 678 521 411 274 234 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 463 493 463
Size: 30 3482
BseYI C'CCAG_C
Cuts at: 937 1695 2272 2768 937
Size: 758 577 496 1681
Fragments arranged by size:
1681 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1054 1100 2308 104
Size: 950 46 1208 1308
Fragments arranged by size:
1308 1208 950 46
BsiHKAI G_wGCw'C
Cuts at: 290 526 1304 1494 2286 290
Size: 236 778 190 792 1516
Fragments arranged by size:
1516 792 778 236 190
BsmI GAATG_Cn'
Cuts at: 502 502
Size: 3512
Bsp1286I G_dGCh'C
Cuts at: 290 311 526 1137 1230 1304 1494 1556 2286
Size: 21 215 611 93 74 190 62 730
Cuts at: 2286 290
Size: 1516
Fragments arranged by size:
1516 730 611 215 190 93 74 62
21
BspEI T'CCGG_A
Cuts at: 2732 2732
Size: 3512
BspHI T'CATG_A
Cuts at: 2688 2688
Size: 3512
BspMI ACCTGCnnnn'nnnn_
Cuts at: 562 776 1078 1459 562
Size: 214 302 381 2615
Fragments arranged by size:
2615 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1804 1858 3342 3507 50
Size: 1754 54 1484 165 55
Fragments arranged by size:
1754 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1424 1424
Size: 3512
BsrFI r'CCGG_y
Cuts at: 395 467 1510 1691 1900 395
Size: 72 1043 181 209 2007
Fragments arranged by size:
2007 1043 209 181 72
BsrGI T'GTAC_A
Cuts at: 263 263
Size: 3512
BssSI C'ACGA_G
Cuts at: 1783 2141 1783
Size: 358 3154
BstAPI GCAn_nnn'nTGC
Cuts at: 889 889
Size: 3512
BstBI TT'CG_AA
Cuts at: 1873 1873
Size: 3512
BstYI r'GATC_y
Cuts at: 1 537 1004 1027 1362 1608 2620 2706 2718
Size: 536 467 23 335 246 1012 86 12
Cuts at: 2718 3064 1
Size: 346 449
Fragments arranged by size:
1012 536 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 447 1462 1643 1806 447
Size: 1015 181 163 2153
Fragments arranged by size:
2153 1015 181 163
BtsI GCAGTG_nn'
Cuts at: 309 387 421 455 486 755 309
Size: 78 34 34 31 269 3066
Fragments arranged by size:
3066 269 78 34 34 31
DraI TTT'AAA
Cuts at: 511 511
Size: 3512
DraIII CAC_nnn'GTG
Cuts at: 240 240
Size: 3512
DrdI GACnn_nn'nnGTC
Cuts at: 1218 2076 1218
Size: 858 2654
EaeI y'GGCC_r
Cuts at: 293 1097 1271 1662 1689 3034 293
Size: 804 174 391 27 1345 771
Fragments arranged by size:
1345 804 771 391 174 27
EagI C'GGCC_G
Cuts at: 1097 1097
Size: 3512
EarI CTCTTCn'nnn_
Cuts at: 1535 1745 1535
Size: 210 3302
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1720 2030 2176 3322 1720
Size: 310 146 1146 1910
Fragments arranged by size:
1910 1146 310 146
EcoICRI GAG'CTC
Cuts at: 524 524
Size: 3512
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 436 1337 1769 2516 2784 436
Size: 901 432 747 268 1164
Fragments arranged by size:
1164 901 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 651 651
Size: 3512
EcoRI G'AATT_C
Cuts at: 516 516
Size: 3512
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 387 1531 387
Size: 1144 2368
FspI TGC'GCA
Cuts at: 1293 1293
Size: 3512
HaeII r_GCGC'y
Cuts at: 946 1194 2216 3195 946
Size: 248 1022 979 1263
Fragments arranged by size:
1263 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1354 1384 1386 1416 1354
Size: 30 2 30 3450
Fragments arranged by size:
3450 30 30 2
HincII GTy'rAC
Cuts at: 551 551
Size: 3512
KasI G'GCGC_C
Cuts at: 942 1190 942
Size: 248 3264
KpnI G_GTAC'C
Cuts at: 532 532
Size: 3512
MluI A'CGCG_T
Cuts at: 2945 2945
Size: 3512
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2183 2367 2183
Size: 184 3328
MscI TGG'CCA
Cuts at: 295 1273 295
Size: 978 2534
MslI CAynn'nnrTG
Cuts at: 236 1501 1628 236
Size: 1265 127 2120
Fragments arranged by size:
2120 1265 127
MspA1I CmG'CkG
Cuts at: 623 937 1297 2310 2555 623
Size: 314 360 1013 245 1580
Fragments arranged by size:
1580 1013 360 314 245
NaeI GCC'GGC
Cuts at: 397 1693 397
Size: 1296 2216
NarI GG'CG_CC
Cuts at: 943 1191 943
Size: 248 3264
NgoMIV G'CCGG_C
Cuts at: 395 1691 395
Size: 1296 2216
NspI r_CATG'y
Cuts at: 121 450 565 1065 1972 121
Size: 329 115 500 907 1661
Fragments arranged by size:
1661 907 500 329 115
PciI A'CATG_T
Cuts at: 117 1968 117
Size: 1851 1661
PflMI CCAn_nnn'nTGG
Cuts at: 240 240
Size: 3512
PmeI GTTT'AAAC
Cuts at: 511 511
Size: 3512
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2651 2683 2651
Size: 32 3480
PstI C_TGCA'G
Cuts at: 316 559 1244 316
Size: 243 685 2584
Fragments arranged by size:
2584 685 243
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3512
PvuII CAG'CTG
Cuts at: 937 1297 937
Size: 360 3152
RsrII CG'GwC_CG
Cuts at: 1707 1707
Size: 3512
SacI G_AGCT'C
Cuts at: 526 526
Size: 3512
SalI G'TCGA_C
Cuts at: 549 549
Size: 3512
SapI GCTCTTCn'nnn_
Cuts at: 1535 1745 1535
Size: 210 3302
SbfI CC_TGCA'GG
Cuts at: 559 559
Size: 3512
ScaI AGT'ACT
Cuts at: 3029 3029
Size: 3512
SfcI C'TryA_G
Cuts at: 33 312 555 1240 2233 2424 33
Size: 279 243 685 993 191 1121
Fragments arranged by size:
1121 993 685 279 243 191
SfoI GGC'GCC
Cuts at: 944 1192 944
Size: 248 3264
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3512
SmaI CCC'GGG
Cuts at: 534 534
Size: 3512
SmlI C'TyrA_G
Cuts at: 361 667 1582 2074 2336 361
Size: 306 915 492 262 1537
Fragments arranged by size:
1537 915 492 306 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3512
SphI G_CATG'C
Cuts at: 450 565 1065 450
Size: 115 500 2897
Fragments arranged by size:
2897 500 115
StyI C'CwwG_G
Cuts at: 646 1000 646
Size: 354 3158
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 272 1095 1228 1687 1896 272
Size: 823 133 459 209 1888
Fragments arranged by size:
1888 823 459 209 133
TatI w'GTAC_w
Cuts at: 120 263 3027 120
Size: 143 2764 605
Fragments arranged by size:
2764 605 143
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3029 3061 3029
Size: 32 3480
Tth111I GACn'n_nGTC
Cuts at: 1309 1309
Size: 3512
XbaI T'CTAG_A
Cuts at: 543 543
Size: 3512
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1007 1007
Size: 3512
XmaI C'CCGG_G
Cuts at: 532 532
Size: 3512
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglII BlpI Bme1580I BmrI BplI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PciI PflMI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BglI BmgBI BmtI BpmI BseRI BsgI BsiWI BsmBI
BssHII BstEII BstXI BstZ17I Bsu36I BtgI ClaI CspCI
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PfoI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI