Restriction map for
pF1KE0171
(Circular) (Six-base) MAPSORT of: pF1KE0171.seq Check: 7355 from: 1 to: 3779
pF1KE0171 3779 bp
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AccI GT'mk_AC
Cuts at: 817 817
Size: 3779
Acc65I G'GTAC_C
Cuts at: 795 795
Size: 3779
AclI AA'CG_TT
Cuts at: 3619 3619
Size: 3779
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 343 391 664 1604 2036 2783 3051 343
Size: 48 273 940 432 747 268 1071
Fragments arranged by size:
1071 940 747 432 273 268 48
AflIII A'CryG_T
Cuts at: 1131 2235 3212 1131
Size: 1104 977 1698
Fragments arranged by size:
1698 1104 977
AgeI A'CCGG_T
Cuts at: 2167 2167
Size: 3779
AlwNI CAG_nnn'CTG
Cuts at: 1084 2651 1084
Size: 1567 2212
ApaLI G'TGCA_C
Cuts at: 2549 2549
Size: 3779
ApoI r'AATT_y
Cuts at: 783 3132 3388 783
Size: 2349 256 1174
Fragments arranged by size:
2349 1174 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3779
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3779
AvaI C'yCGr_G
Cuts at: 799 799
Size: 3779
BamHI G'GATC_C
Cuts at: 609 804 609
Size: 195 3584
BanI G'GyrC_C
Cuts at: 130 214 795 1209 1457 1492 130
Size: 84 581 414 248 35 2417
Fragments arranged by size:
2417 581 414 248 84 35
BanII G_rGCy'C
Cuts at: 172 793 1823 172
Size: 621 1030 2128
Fragments arranged by size:
2128 1030 621
BbeI G_GCGC'C
Cuts at: 218 1213 1461 218
Size: 995 248 2536
Fragments arranged by size:
2536 995 248
BbvCI CC'TCA_GC
Cuts at: 421 421
Size: 3779
BciVI GTATCCnnnnn_n'
Cuts at: 1677 2438 3779 1677
Size: 761 1341 1677
Fragments arranged by size:
1677 1341 761
BclI T'GATC_A
Cuts at: 1299 1299
Size: 3779
BglII A'GATC_T
Cuts at: 1294 3331 1294
Size: 2037 1742
BlpI GC'TnA_GC
Cuts at: 891 891
Size: 3779
Bme1580I G_kGCm'C
Cuts at: 1404 1497 2553 1404
Size: 93 1056 2630
Fragments arranged by size:
2630 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 744 991 1072 1407 3551 744
Size: 247 81 335 2144 972
Fragments arranged by size:
2144 972 335 247 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2146 2146
Size: 3779
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 156 156
Size: 3779
Bpu10I CC'TnA_GC
Cuts at: 421 540 421
Size: 119 3660
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 955 1834 2326 2624 955
Size: 879 492 298 2110
Fragments arranged by size:
2110 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3483 3483
Size: 3779
BsaAI yAC'GTr
Cuts at: 282 1132 282
Size: 850 2929
BsaBI GATnn'nnATC
Cuts at: 6 1293 3330 6
Size: 1287 2037 455
Fragments arranged by size:
2037 1287 455
BsaHI Gr'CG_yC
Cuts at: 215 1210 1458 2160 3656 215
Size: 995 248 702 1496 338
Fragments arranged by size:
1496 995 702 338 248
BsaWI w'CCGG_w
Cuts at: 150 968 1489 2167 2441 2588 2999 150
Size: 818 521 678 274 147 411 930
Fragments arranged by size:
930 818 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 319 319
Size: 3779
BseYI C'CCAG_C
Cuts at: 645 1204 1962 2539 3035 645
Size: 559 758 577 496 1389
Fragments arranged by size:
1389 758 577 559 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 238 350 238
Size: 112 3667
BsiEI CG_ry'CG
Cuts at: 104 1321 1367 2575 104
Size: 1217 46 1208 1308
Fragments arranged by size:
1308 1217 1208 46
BsiHKAI G_wGCw'C
Cuts at: 668 793 1571 1761 2553 668
Size: 125 778 190 792 1894
Fragments arranged by size:
1894 792 778 190 125
Bsp1286I G_dGCh'C
Cuts at: 172 668 793 1404 1497 1571 1761 1823 2553
Size: 496 125 611 93 74 190 62 730
Cuts at: 2553 172
Size: 1398
Fragments arranged by size:
1398 730 611 496 190 125 93 74
62
BspEI T'CCGG_A
Cuts at: 2999 2999
Size: 3779
BspHI T'CATG_A
Cuts at: 2955 2955
Size: 3779
BspMI ACCTGCnnnn'nnnn_
Cuts at: 206 829 1043 1345 1726 206
Size: 623 214 302 381 2259
Fragments arranged by size:
2259 623 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2071 2125 3609 3774 50
Size: 2021 54 1484 165 55
Fragments arranged by size:
2021 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1691 1691
Size: 3779
BsrFI r'CCGG_y
Cuts at: 1777 1958 2167 1777
Size: 181 209 3389
Fragments arranged by size:
3389 209 181
BssSI C'ACGA_G
Cuts at: 2050 2408 2050
Size: 358 3421
BstAPI GCAn_nnn'nTGC
Cuts at: 246 1156 246
Size: 910 2869
BstBI TT'CG_AA
Cuts at: 2140 2140
Size: 3779
BstYI r'GATC_y
Cuts at: 1 609 804 1271 1294 1629 1875 2887 2973
Size: 608 195 467 23 335 246 1012 86
Cuts at: 2973 2985 3331 1
Size: 12 346 449
Fragments arranged by size:
1012 608 467 449 346 335 246 195
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1729 1910 2073 1729
Size: 181 163 3435
Fragments arranged by size:
3435 181 163
BtsI GCAGTG_nn'
Cuts at: 128 613 640 1022 128
Size: 485 27 382 2885
Fragments arranged by size:
2885 485 382 27
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 366 401 366
Size: 35 3744
DraI TTT'AAA
Cuts at: 778 778
Size: 3779
DrdI GACnn_nn'nnGTC
Cuts at: 1485 2343 1485
Size: 858 2921
EaeI y'GGCC_r
Cuts at: 326 1364 1538 1929 1956 3301 326
Size: 1038 174 391 27 1345 804
Fragments arranged by size:
1345 1038 804 391 174 27
EagI C'GGCC_G
Cuts at: 1364 1364
Size: 3779
EarI CTCTTCn'nnn_
Cuts at: 362 1802 2012 362
Size: 1440 210 2129
Fragments arranged by size:
2129 1440 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1987 2297 2443 3589 1987
Size: 310 146 1146 2177
Fragments arranged by size:
2177 1146 310 146
EcoICRI GAG'CTC
Cuts at: 791 791
Size: 3779
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 156 343 391 664 1604 2036 2783 3051 156
Size: 187 48 273 940 432 747 268 884
Fragments arranged by size:
940 884 747 432 273 268 187 48
EcoO109I rG'GnC_Cy
Cuts at: 918 918
Size: 3779
EcoRI G'AATT_C
Cuts at: 783 783
Size: 3779
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1798 1798
Size: 3779
FspI TGC'GCA
Cuts at: 1560 1560
Size: 3779
HaeII r_GCGC'y
Cuts at: 218 1213 1461 2483 3462 218
Size: 995 248 1022 979 535
Fragments arranged by size:
1022 995 979 535 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1621 1651 1653 1683 1621
Size: 30 2 30 3717
Fragments arranged by size:
3717 30 30 2
HincII GTy'rAC
Cuts at: 818 818
Size: 3779
KasI G'GCGC_C
Cuts at: 214 1209 1457 214
Size: 995 248 2536
Fragments arranged by size:
2536 995 248
KpnI G_GTAC'C
Cuts at: 799 799
Size: 3779
MluI A'CGCG_T
Cuts at: 3212 3212
Size: 3779
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2450 2634 2450
Size: 184 3595
MscI TGG'CCA
Cuts at: 328 1540 328
Size: 1212 2567
MslI CAynn'nnrTG
Cuts at: 1768 1895 1768
Size: 127 3652
MspA1I CmG'CkG
Cuts at: 144 252 890 1204 1564 2577 2822 144
Size: 108 638 314 360 1013 245 1101
Fragments arranged by size:
1101 1013 638 360 314 245 108
NaeI GCC'GGC
Cuts at: 1960 1960
Size: 3779
NarI GG'CG_CC
Cuts at: 215 1210 1458 215
Size: 995 248 2536
Fragments arranged by size:
2536 995 248
NgoMIV G'CCGG_C
Cuts at: 1958 1958
Size: 3779
NspI r_CATG'y
Cuts at: 832 1332 2239 832
Size: 500 907 2372
Fragments arranged by size:
2372 907 500
PciI A'CATG_T
Cuts at: 2235 2235
Size: 3779
PmeI GTTT'AAAC
Cuts at: 778 778
Size: 3779
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2918 2950 2918
Size: 32 3747
PstI C_TGCA'G
Cuts at: 826 1511 826
Size: 685 3094
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3779
PvuII CAG'CTG
Cuts at: 144 252 1204 1564 144
Size: 108 952 360 2359
Fragments arranged by size:
2359 952 360 108
RsrII CG'GwC_CG
Cuts at: 1974 1974
Size: 3779
SacI G_AGCT'C
Cuts at: 793 793
Size: 3779
SalI G'TCGA_C
Cuts at: 816 816
Size: 3779
SapI GCTCTTCn'nnn_
Cuts at: 362 1802 2012 362
Size: 1440 210 2129
Fragments arranged by size:
2129 1440 210
SbfI CC_TGCA'GG
Cuts at: 826 826
Size: 3779
ScaI AGT'ACT
Cuts at: 3296 3296
Size: 3779
SfcI C'TryA_G
Cuts at: 33 822 1507 2500 2691 33
Size: 789 685 993 191 1121
Fragments arranged by size:
1121 993 789 685 191
SfoI GGC'GCC
Cuts at: 216 1211 1459 216
Size: 995 248 2536
Fragments arranged by size:
2536 995 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3779
SmaI CCC'GGG
Cuts at: 801 801
Size: 3779
SmlI C'TyrA_G
Cuts at: 934 1849 2341 2603 934
Size: 915 492 262 2110
Fragments arranged by size:
2110 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3779
SphI G_CATG'C
Cuts at: 832 1332 832
Size: 500 3279
StuI AGG'CCT
Cuts at: 632 699 632
Size: 67 3712
StyI C'CwwG_G
Cuts at: 329 534 913 1267 329
Size: 205 379 354 2841
Fragments arranged by size:
2841 379 354 205
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1362 1495 1954 2163 1362
Size: 133 459 209 2978
Fragments arranged by size:
2978 459 209 133
TatI w'GTAC_w
Cuts at: 3294 3294
Size: 3779
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3296 3328 3296
Size: 32 3747
Tth111I GACn'n_nGTC
Cuts at: 1576 1576
Size: 3779
XbaI T'CTAG_A
Cuts at: 810 810
Size: 3779
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 141 1274 141
Size: 1133 2646
XmaI C'CCGG_G
Cuts at: 799 799
Size: 3779
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbvCI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PciI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BcgI BfrBI BglI
BmgBI BmtI BsaXI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I BtgI ClaI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI XmnI ZraI