Restriction map for
pF1KE0174
(Circular) (Six-base) MAPSORT of: pF1KE0174.seq Check: 4417 from: 1 to: 4031
pF1KE0174 4031 bp
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AatII G_ACGT'C
Cuts at: 268 268
Size: 4031
AccI GT'mk_AC
Cuts at: 255 1069 255
Size: 814 3217
Acc65I G'GTAC_C
Cuts at: 144 1047 144
Size: 903 3128
AclI AA'CG_TT
Cuts at: 755 1025 3871 755
Size: 270 2846 915
Fragments arranged by size:
2846 915 270
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 758 1856 2288 3035 3303 758
Size: 1098 432 747 268 1486
Fragments arranged by size:
1486 1098 747 432 268
AflIII A'CryG_T
Cuts at: 1383 2487 3464 1383
Size: 1104 977 1950
Fragments arranged by size:
1950 1104 977
AgeI A'CCGG_T
Cuts at: 2419 2419
Size: 4031
AleI CACnn'nnGTG
Cuts at: 910 910
Size: 4031
AlwNI CAG_nnn'CTG
Cuts at: 1336 2903 1336
Size: 1567 2464
ApaLI G'TGCA_C
Cuts at: 2801 2801
Size: 4031
ApoI r'AATT_y
Cuts at: 1035 3384 3640 1035
Size: 2349 256 1426
Fragments arranged by size:
2349 1426 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4031
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4031
AvaI C'yCGr_G
Cuts at: 1051 1051
Size: 4031
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 638 671 638
Size: 33 3998
BamHI G'GATC_C
Cuts at: 1056 1056
Size: 4031
BanI G'GyrC_C
Cuts at: 144 231 1047 1461 1709 1744 144
Size: 87 816 414 248 35 2431
Fragments arranged by size:
2431 816 414 248 87 35
BanII G_rGCy'C
Cuts at: 1045 2075 1045
Size: 1030 3001
BbeI G_GCGC'C
Cuts at: 235 1465 1713 235
Size: 1230 248 2553
Fragments arranged by size:
2553 1230 248
BbvCI CC'TCA_GC
Cuts at: 199 224 199
Size: 25 4006
BciVI GTATCCnnnnn_n'
Cuts at: 212 1929 2690 4031 212
Size: 1717 761 1341 212
Fragments arranged by size:
1717 1341 761 212
BclI T'GATC_A
Cuts at: 939 1551 939
Size: 612 3419
BglI GCCn_nnn'nGGC
Cuts at: 240 240
Size: 4031
BglII A'GATC_T
Cuts at: 1546 3583 1546
Size: 2037 1994
BlpI GC'TnA_GC
Cuts at: 1143 1143
Size: 4031
Bme1580I G_kGCm'C
Cuts at: 1656 1749 2805 1656
Size: 93 1056 2882
Fragments arranged by size:
2882 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 496 1243 1324 1659 3803 496
Size: 747 81 335 2144 724
Fragments arranged by size:
2144 747 724 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2398 2398
Size: 4031
Bpu10I CC'TnA_GC
Cuts at: 199 224 199
Size: 25 4006
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1207 2086 2578 2876 1207
Size: 879 492 298 2362
Fragments arranged by size:
2362 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3735 3735
Size: 4031
BsaAI yAC'GTr
Cuts at: 1384 1384
Size: 4031
BsaBI GATnn'nnATC
Cuts at: 6 1545 3582 6
Size: 1539 2037 455
Fragments arranged by size:
2037 1539 455
BsaHI Gr'CG_yC
Cuts at: 232 265 1462 1710 2412 3908 232
Size: 33 1197 248 702 1496 355
Fragments arranged by size:
1496 1197 702 355 248 33
BsaWI w'CCGG_w
Cuts at: 1220 1741 2419 2693 2840 3251 1220
Size: 521 678 274 147 411 2000
Fragments arranged by size:
2000 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 227 257 227
Size: 30 4001
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 412 412
Size: 4031
BseYI C'CCAG_C
Cuts at: 158 164 246 1456 2214 2791 3287 158
Size: 6 82 1210 758 577 496 902
Fragments arranged by size:
1210 902 758 577 496 82 6
BsiEI CG_ry'CG
Cuts at: 104 894 1573 1619 2827 104
Size: 790 679 46 1208 1308
Fragments arranged by size:
1308 1208 790 679 46
BsiHKAI G_wGCw'C
Cuts at: 1045 1823 2013 2805 1045
Size: 778 190 792 2271
Fragments arranged by size:
2271 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 1045 1656 1749 1823 2013 2075 2805 1045
Size: 611 93 74 190 62 730 2271
Fragments arranged by size:
2271 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3251 3251
Size: 4031
BspHI T'CATG_A
Cuts at: 3207 3207
Size: 4031
BspMI ACCTGCnnnn'nnnn_
Cuts at: 873 1081 1295 1597 1978 873
Size: 208 214 302 381 2926
Fragments arranged by size:
2926 381 302 214 208
BsrBI CCG'CTC
Cuts at: 50 2323 2377 3861 4026 50
Size: 2273 54 1484 165 55
Fragments arranged by size:
2273 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1943 1943
Size: 4031
BsrFI r'CCGG_y
Cuts at: 2029 2210 2419 2029
Size: 181 209 3641
Fragments arranged by size:
3641 209 181
BssHII G'CGCG_C
Cuts at: 118 118
Size: 4031
BssSI C'ACGA_G
Cuts at: 2302 2660 2302
Size: 358 3673
BstAPI GCAn_nnn'nTGC
Cuts at: 1408 1408
Size: 4031
BstBI TT'CG_AA
Cuts at: 2392 2392
Size: 4031
BstYI r'GATC_y
Cuts at: 1 556 1056 1523 1546 1881 2127 3139 3225
Size: 555 500 467 23 335 246 1012 86
Cuts at: 3225 3237 3583 1
Size: 12 346 449
Fragments arranged by size:
1012 555 500 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 192 235 840 107
Size: 85 43 605 3298
Fragments arranged by size:
3298 605 85 43
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1981 2162 2325 1981
Size: 181 163 3687
Fragments arranged by size:
3687 181 163
BtsI GCAGTG_nn'
Cuts at: 789 1274 789
Size: 485 3546
DraI TTT'AAA
Cuts at: 1030 1030
Size: 4031
DrdI GACnn_nn'nnGTC
Cuts at: 1737 2595 1737
Size: 858 3173
EaeI y'GGCC_r
Cuts at: 1616 1790 2181 2208 3553 1616
Size: 174 391 27 1345 2094
Fragments arranged by size:
2094 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1616 1616
Size: 4031
EarI CTCTTCn'nnn_
Cuts at: 2054 2264 2054
Size: 210 3821
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2239 2549 2695 3841 2239
Size: 310 146 1146 2429
Fragments arranged by size:
2429 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1043 1043
Size: 4031
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 758 1856 2288 3035 3303 758
Size: 1098 432 747 268 1486
Fragments arranged by size:
1486 1098 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 429 429
Size: 4031
EcoO109I rG'GnC_Cy
Cuts at: 1170 1170
Size: 4031
EcoRI G'AATT_C
Cuts at: 1035 1035
Size: 4031
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 526 2050 526
Size: 1524 2507
FspI TGC'GCA
Cuts at: 1812 1812
Size: 4031
HaeII r_GCGC'y
Cuts at: 235 1465 1713 2735 3714 235
Size: 1230 248 1022 979 552
Fragments arranged by size:
1230 1022 979 552 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 454 486 994 1026 1873 1903 1905 1935 454
Size: 32 508 32 847 30 2 30 2550
Fragments arranged by size:
2550 847 508 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1023 1070 1023
Size: 47 3984
HpaI GTT'AAC
Cuts at: 1023 1023
Size: 4031
KasI G'GCGC_C
Cuts at: 231 1461 1709 231
Size: 1230 248 2553
Fragments arranged by size:
2553 1230 248
KpnI G_GTAC'C
Cuts at: 148 1051 148
Size: 903 3128
MfeI C'AATT_G
Cuts at: 593 593
Size: 4031
MluI A'CGCG_T
Cuts at: 3464 3464
Size: 4031
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 133 432 534 2702 2886 133
Size: 299 102 2168 184 1278
Fragments arranged by size:
2168 1278 299 184 102
MscI TGG'CCA
Cuts at: 1792 1792
Size: 4031
MslI CAynn'nnrTG
Cuts at: 910 2020 2147 910
Size: 1110 127 2794
Fragments arranged by size:
2794 1110 127
MspA1I CmG'CkG
Cuts at: 130 228 640 1142 1456 1816 2829 3074 130
Size: 98 412 502 314 360 1013 245 1087
Fragments arranged by size:
1087 1013 502 412 360 314 245 98
NaeI GCC'GGC
Cuts at: 2212 2212
Size: 4031
NarI GG'CG_CC
Cuts at: 232 1462 1710 232
Size: 1230 248 2553
Fragments arranged by size:
2553 1230 248
NcoI C'CATG_G
Cuts at: 107 840 107
Size: 733 3298
NgoMIV G'CCGG_C
Cuts at: 2210 2210
Size: 4031
NspI r_CATG'y
Cuts at: 723 1084 1584 2491 723
Size: 361 500 907 2263
Fragments arranged by size:
2263 907 500 361
PciI A'CATG_T
Cuts at: 2487 2487
Size: 4031
PfoI T'CCnGG_A
Cuts at: 333 333
Size: 4031
PmeI GTTT'AAAC
Cuts at: 1030 1030
Size: 4031
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3170 3202 3170
Size: 32 3999
PsiI TTA'TAA
Cuts at: 311 752 311
Size: 441 3590
PstI C_TGCA'G
Cuts at: 645 1078 1763 645
Size: 433 685 2913
Fragments arranged by size:
2913 685 433
PvuI CG_AT'CG
Cuts at: 104 894 104
Size: 790 3241
PvuII CAG'CTG
Cuts at: 130 640 1456 1816 130
Size: 510 816 360 2345
Fragments arranged by size:
2345 816 510 360
RsrII CG'GwC_CG
Cuts at: 2226 2226
Size: 4031
SacI G_AGCT'C
Cuts at: 1045 1045
Size: 4031
SalI G'TCGA_C
Cuts at: 1068 1068
Size: 4031
SapI GCTCTTCn'nnn_
Cuts at: 2054 2264 2054
Size: 210 3821
SbfI CC_TGCA'GG
Cuts at: 1078 1078
Size: 4031
ScaI AGT'ACT
Cuts at: 899 3548 899
Size: 2649 1382
SfcI C'TryA_G
Cuts at: 33 635 641 1016 1074 1759 2752 2943 33
Size: 602 6 375 58 685 993 191 1121
Fragments arranged by size:
1121 993 685 602 375 191 58 6
SfoI GGC'GCC
Cuts at: 233 1463 1711 233
Size: 1230 248 2553
Fragments arranged by size:
2553 1230 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4031
SmaI CCC'GGG
Cuts at: 1053 1053
Size: 4031
SmlI C'TyrA_G
Cuts at: 1186 2101 2593 2855 1186
Size: 915 492 262 2362
Fragments arranged by size:
2362 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4031
SphI G_CATG'C
Cuts at: 723 1084 1584 723
Size: 361 500 3170
Fragments arranged by size:
3170 500 361
StyI C'CwwG_G
Cuts at: 107 564 840 865 1165 1519 107
Size: 457 276 25 300 354 2619
Fragments arranged by size:
2619 457 354 300 276 25
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 172 1614 1747 2206 2415 172
Size: 1442 133 459 209 1788
Fragments arranged by size:
1788 1442 459 209 133
TatI w'GTAC_w
Cuts at: 761 897 3546 761
Size: 136 2649 1246
Fragments arranged by size:
2649 1246 136
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 227 259 260 292 3548 3580 227
Size: 32 1 32 3256 32 678
Fragments arranged by size:
3256 678 32 32 32 1
Tth111I GACn'n_nGTC
Cuts at: 1828 1828
Size: 4031
XbaI T'CTAG_A
Cuts at: 1062 1062
Size: 4031
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1526 1526
Size: 4031
XmaI C'CCGG_G
Cuts at: 1051 1051
Size: 4031
ZraI GAC'GTC
Cuts at: 266 266
Size: 4031
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PfoI PmeI
PpiI PsiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AlfI AloI ApaI AscI
AvrII BbsI BcgI BfrBI BmgBI BmtI BpmI BsgI
BsiWI BsmI BsmBI BsrGI BstEII BstXI BstZ17I Bsu36I
ClaI CspCI DraIII EcoRV FseI FspAI HindIII NdeI
NheI NotI NruI NsiI PacI PasI PflMI PmlI
PpuMI PshAI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI