Restriction map for
pF1KE0177
(Circular) (Six-base) MAPSORT of: pF1KE0177.seq Check: 7491 from: 1 to: 3845
pF1KE0177 3845 bp
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AccI GT'mk_AC
Cuts at: 883 883
Size: 3845
Acc65I G'GTAC_C
Cuts at: 861 861
Size: 3845
AclI AA'CG_TT
Cuts at: 3685 3685
Size: 3845
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 429 1670 2102 2849 3117 429
Size: 1241 432 747 268 1157
Fragments arranged by size:
1241 1157 747 432 268
AflIII A'CryG_T
Cuts at: 1197 2301 3278 1197
Size: 1104 977 1764
Fragments arranged by size:
1764 1104 977
AgeI A'CCGG_T
Cuts at: 2233 2233
Size: 3845
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 727 727
Size: 3845
AlwNI CAG_nnn'CTG
Cuts at: 305 541 1150 2717 305
Size: 236 609 1567 1433
Fragments arranged by size:
1567 1433 609 236
ApaLI G'TGCA_C
Cuts at: 2615 2615
Size: 3845
ApoI r'AATT_y
Cuts at: 849 3198 3454 849
Size: 2349 256 1240
Fragments arranged by size:
2349 1240 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3845
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3845
AvaI C'yCGr_G
Cuts at: 865 865
Size: 3845
BamHI G'GATC_C
Cuts at: 870 870
Size: 3845
BanI G'GyrC_C
Cuts at: 403 456 861 1275 1523 1558 403
Size: 53 405 414 248 35 2690
Fragments arranged by size:
2690 414 405 248 53 35
BanII G_rGCy'C
Cuts at: 859 1889 859
Size: 1030 2815
BbeI G_GCGC'C
Cuts at: 1279 1527 1279
Size: 248 3597
BbvCI CC'TCA_GC
Cuts at: 382 382
Size: 3845
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 375 409 375
Size: 34 3811
BciVI GTATCCnnnnn_n'
Cuts at: 1743 2504 3845 1743
Size: 761 1341 1743
Fragments arranged by size:
1743 1341 761
BclI T'GATC_A
Cuts at: 221 1365 221
Size: 1144 2701
BfrBI ATG'CAT
Cuts at: 759 759
Size: 3845
BglII A'GATC_T
Cuts at: 1360 3397 1360
Size: 2037 1808
BlpI GC'TnA_GC
Cuts at: 957 957
Size: 3845
Bme1580I G_kGCm'C
Cuts at: 285 408 461 1470 1563 2619 285
Size: 123 53 1009 93 1056 1511
Fragments arranged by size:
1511 1056 1009 123 93 53
BmrI ACTGGGnnnn_n'
Cuts at: 746 1057 1138 1473 3617 746
Size: 311 81 335 2144 974
Fragments arranged by size:
2144 974 335 311 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2212 2212
Size: 3845
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 225 349 225
Size: 124 3721
Bpu10I CC'TnA_GC
Cuts at: 382 382
Size: 3845
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1021 1900 2392 2690 1021
Size: 879 492 298 2176
Fragments arranged by size:
2176 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3549 3549
Size: 3845
BsaAI yAC'GTr
Cuts at: 1198 1198
Size: 3845
BsaBI GATnn'nnATC
Cuts at: 6 1359 3396 6
Size: 1353 2037 455
Fragments arranged by size:
2037 1353 455
BsaHI Gr'CG_yC
Cuts at: 1276 1524 2226 3722 1276
Size: 248 702 1496 1399
Fragments arranged by size:
1496 1399 702 248
BsaWI w'CCGG_w
Cuts at: 1034 1555 2233 2507 2654 3065 1034
Size: 521 678 274 147 411 1814
Fragments arranged by size:
1814 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 514 652 1270 2028 2605 3101 514
Size: 138 618 758 577 496 1258
Fragments arranged by size:
1258 758 618 577 496 138
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 515 515
Size: 3845
BsiEI CG_ry'CG
Cuts at: 104 1387 1433 2641 104
Size: 1283 46 1208 1308
Fragments arranged by size:
1308 1283 1208 46
BsiHKAI G_wGCw'C
Cuts at: 859 1637 1827 2619 859
Size: 778 190 792 2085
Fragments arranged by size:
2085 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 285 408 461 859 1470 1563 1637 1827 1889
Size: 123 53 398 611 93 74 190 62
Cuts at: 1889 2619 285
Size: 730 1511
Fragments arranged by size:
1511 730 611 398 190 123 93 74
62 53
BspEI T'CCGG_A
Cuts at: 3065 3065
Size: 3845
BspHI T'CATG_A
Cuts at: 3021 3021
Size: 3845
BspMI ACCTGCnnnn'nnnn_
Cuts at: 362 487 526 895 1109 1411 1792 362
Size: 125 39 369 214 302 381 2415
Fragments arranged by size:
2415 381 369 302 214 125 39
BsrBI CCG'CTC
Cuts at: 50 2137 2191 3675 3840 50
Size: 2087 54 1484 165 55
Fragments arranged by size:
2087 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1757 1757
Size: 3845
BsrFI r'CCGG_y
Cuts at: 1843 2024 2233 1843
Size: 181 209 3455
Fragments arranged by size:
3455 209 181
BsrGI T'GTAC_A
Cuts at: 697 697
Size: 3845
BssSI C'ACGA_G
Cuts at: 2116 2474 2116
Size: 358 3487
BstAPI GCAn_nnn'nTGC
Cuts at: 305 1222 305
Size: 917 2928
BstBI TT'CG_AA
Cuts at: 2206 2206
Size: 3845
BstXI CCAn_nnnn'nTGG
Cuts at: 483 563 483
Size: 80 3765
BstYI r'GATC_y
Cuts at: 1 870 1337 1360 1695 1941 2953 3039 3051
Size: 869 467 23 335 246 1012 86 12
Cuts at: 3051 3397 1
Size: 346 449
Fragments arranged by size:
1012 869 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 398 107
Size: 291 3554
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1795 1976 2139 1795
Size: 181 163 3501
Fragments arranged by size:
3501 181 163
BtsI GCAGTG_nn'
Cuts at: 306 437 635 1088 306
Size: 131 198 453 3063
Fragments arranged by size:
3063 453 198 131
DraI TTT'AAA
Cuts at: 844 844
Size: 3845
DraIII CAC_nnn'GTG
Cuts at: 510 510
Size: 3845
DrdI GACnn_nn'nnGTC
Cuts at: 358 1551 2409 358
Size: 1193 858 1794
Fragments arranged by size:
1794 1193 858
EaeI y'GGCC_r
Cuts at: 473 1430 1604 1995 2022 3367 473
Size: 957 174 391 27 1345 951
Fragments arranged by size:
1345 957 951 391 174 27
EagI C'GGCC_G
Cuts at: 1430 1430
Size: 3845
EarI CTCTTCn'nnn_
Cuts at: 1868 2078 1868
Size: 210 3635
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2053 2363 2509 3655 2053
Size: 310 146 1146 2243
Fragments arranged by size:
2243 1146 310 146
EcoICRI GAG'CTC
Cuts at: 857 857
Size: 3845
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 225 349 429 1670 2102 2849 3117 225
Size: 124 80 1241 432 747 268 953
Fragments arranged by size:
1241 953 747 432 268 124 80
EcoO109I rG'GnC_Cy
Cuts at: 449 657 984 449
Size: 208 327 3310
Fragments arranged by size:
3310 327 208
EcoRI G'AATT_C
Cuts at: 849 849
Size: 3845
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1864 1864
Size: 3845
FspI TGC'GCA
Cuts at: 1626 1626
Size: 3845
HaeII r_GCGC'y
Cuts at: 1279 1527 2549 3528 1279
Size: 248 1022 979 1596
Fragments arranged by size:
1596 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1687 1717 1719 1749 1687
Size: 30 2 30 3783
Fragments arranged by size:
3783 30 30 2
HincII GTy'rAC
Cuts at: 342 884 342
Size: 542 3303
KasI G'GCGC_C
Cuts at: 1275 1523 1275
Size: 248 3597
KpnI G_GTAC'C
Cuts at: 865 865
Size: 3845
MluI A'CGCG_T
Cuts at: 3278 3278
Size: 3845
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2516 2700 2516
Size: 184 3661
MscI TGG'CCA
Cuts at: 475 1606 475
Size: 1131 2714
MslI CAynn'nnrTG
Cuts at: 1834 1961 1834
Size: 127 3718
MspA1I CmG'CkG
Cuts at: 348 956 1270 1630 2643 2888 348
Size: 608 314 360 1013 245 1305
Fragments arranged by size:
1305 1013 608 360 314 245
NaeI GCC'GGC
Cuts at: 2026 2026
Size: 3845
NarI GG'CG_CC
Cuts at: 1276 1524 1276
Size: 248 3597
NcoI C'CATG_G
Cuts at: 107 398 107
Size: 291 3554
NgoMIV G'CCGG_C
Cuts at: 2024 2024
Size: 3845
NsiI A_TGCA'T
Cuts at: 761 761
Size: 3845
NspI r_CATG'y
Cuts at: 390 704 898 1398 2305 390
Size: 314 194 500 907 1930
Fragments arranged by size:
1930 907 500 314 194
PciI A'CATG_T
Cuts at: 2301 2301
Size: 3845
PflMI CCAn_nnn'nTGG
Cuts at: 510 510
Size: 3845
PmeI GTTT'AAAC
Cuts at: 844 844
Size: 3845
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2984 3016 2984
Size: 32 3813
PpuMI rG'GwC_Cy
Cuts at: 449 449
Size: 3845
PsiI TTA'TAA
Cuts at: 578 578
Size: 3845
PstI C_TGCA'G
Cuts at: 892 1577 892
Size: 685 3160
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3845
PvuII CAG'CTG
Cuts at: 348 1270 1630 348
Size: 922 360 2563
Fragments arranged by size:
2563 922 360
RsrII CG'GwC_CG
Cuts at: 2040 2040
Size: 3845
SacI G_AGCT'C
Cuts at: 859 859
Size: 3845
SalI G'TCGA_C
Cuts at: 882 882
Size: 3845
SapI GCTCTTCn'nnn_
Cuts at: 1868 2078 1868
Size: 210 3635
SbfI CC_TGCA'GG
Cuts at: 892 892
Size: 3845
ScaI AGT'ACT
Cuts at: 3362 3362
Size: 3845
SexAI A'CCwGG_T
Cuts at: 506 506
Size: 3845
SfcI C'TryA_G
Cuts at: 33 888 1573 2566 2757 33
Size: 855 685 993 191 1121
Fragments arranged by size:
1121 993 855 685 191
SfoI GGC'GCC
Cuts at: 1277 1525 1277
Size: 248 3597
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3845
SmaI CCC'GGG
Cuts at: 867 867
Size: 3845
SmlI C'TyrA_G
Cuts at: 1000 1915 2407 2669 1000
Size: 915 492 262 2176
Fragments arranged by size:
2176 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3845
SphI G_CATG'C
Cuts at: 390 898 1398 390
Size: 508 500 2837
Fragments arranged by size:
2837 508 500
StuI AGG'CCT
Cuts at: 176 266 176
Size: 90 3755
StyI C'CwwG_G
Cuts at: 107 398 979 1333 107
Size: 291 581 354 2619
Fragments arranged by size:
2619 581 354 291
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 261 391 765 1428 1561 2020 2229 261
Size: 130 374 663 133 459 209 1877
Fragments arranged by size:
1877 663 459 374 209 133 130
TatI w'GTAC_w
Cuts at: 697 737 3360 697
Size: 40 2623 1182
Fragments arranged by size:
2623 1182 40
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3362 3394 3362
Size: 32 3813
Tth111I GACn'n_nGTC
Cuts at: 1642 1642
Size: 3845
XbaI T'CTAG_A
Cuts at: 876 876
Size: 3845
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 468 511 1340 468
Size: 43 829 2973
Fragments arranged by size:
2973 829 43
XmaI C'CCGG_G
Cuts at: 865 865
Size: 3845
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbvCI BcgI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NsiI NspI PciI PflMI PmeI PpiI
PpuMI PsiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbsI BglI BmgBI BmtI BsaXI
BseRI BsiWI BsmI BsmBI BssHII BstEII BstZ17I Bsu36I
ClaI CspCI EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI PacI PasI PfoI
PmlI PshAI PspOMI PspXI PsrI SacII SanDI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI