Restriction map for
pF1KE0179
(Circular) (Six-base) MAPSORT of: pF1KE0179.seq Check: 90 from: 1 to: 4139
pF1KE0179 4139 bp
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AccI GT'mk_AC
Cuts at: 704 1177 704
Size: 473 3666
Acc65I G'GTAC_C
Cuts at: 1155 1155
Size: 4139
AclI AA'CG_TT
Cuts at: 3979 3979
Size: 4139
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1964 2396 3143 3411 1964
Size: 432 747 268 2692
Fragments arranged by size:
2692 747 432 268
AflIII A'CryG_T
Cuts at: 1491 2595 3572 1491
Size: 1104 977 2058
Fragments arranged by size:
2058 1104 977
AgeI A'CCGG_T
Cuts at: 2527 2527
Size: 4139
AlwNI CAG_nnn'CTG
Cuts at: 975 1444 3011 975
Size: 469 1567 2103
Fragments arranged by size:
2103 1567 469
ApaLI G'TGCA_C
Cuts at: 263 2909 263
Size: 2646 1493
ApoI r'AATT_y
Cuts at: 1064 1143 3492 3748 1064
Size: 79 2349 256 1455
Fragments arranged by size:
2349 1455 256 79
AseI AT'TA_AT
Cuts at: 20 20
Size: 4139
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4139
AvaI C'yCGr_G
Cuts at: 132 402 1159 132
Size: 270 757 3112
Fragments arranged by size:
3112 757 270
AvrII C'CTAG_G
Cuts at: 1101 1101
Size: 4139
BamHI G'GATC_C
Cuts at: 1164 1164
Size: 4139
BanI G'GyrC_C
Cuts at: 1155 1569 1817 1852 1155
Size: 414 248 35 3442
Fragments arranged by size:
3442 414 248 35
BanII G_rGCy'C
Cuts at: 196 218 374 627 832 1153 2183 196
Size: 22 156 253 205 321 1030 2152
Fragments arranged by size:
2152 1030 321 253 205 156 22
BbeI G_GCGC'C
Cuts at: 1573 1821 1573
Size: 248 3891
BbsI GAAGACnn'nnnn_
Cuts at: 766 766
Size: 4139
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 393 427 495 529 393
Size: 34 68 34 4003
Fragments arranged by size:
4003 68 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2037 2798 4139 2037
Size: 761 1341 2037
Fragments arranged by size:
2037 1341 761
BclI T'GATC_A
Cuts at: 741 1659 741
Size: 918 3221
BglI GCCn_nnn'nGGC
Cuts at: 200 414 200
Size: 214 3925
BglII A'GATC_T
Cuts at: 1654 3691 1654
Size: 2037 2102
BlpI GC'TnA_GC
Cuts at: 947 1251 947
Size: 304 3835
Bme1580I G_kGCm'C
Cuts at: 180 267 363 1764 1857 2913 180
Size: 87 96 1401 93 1056 1406
Fragments arranged by size:
1406 1401 1056 96 93 87
BmrI ACTGGGnnnn_n'
Cuts at: 507 830 1351 1432 1767 3911 507
Size: 323 521 81 335 2144 735
Fragments arranged by size:
2144 735 521 335 323 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2506 2506
Size: 4139
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 192 206 736 192
Size: 14 530 3595
Fragments arranged by size:
3595 530 14
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1315 2194 2686 2984 1315
Size: 879 492 298 2470
Fragments arranged by size:
2470 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3843 3843
Size: 4139
BsaAI yAC'GTr
Cuts at: 1492 1492
Size: 4139
BsaBI GATnn'nnATC
Cuts at: 6 1653 3690 6
Size: 1647 2037 455
Fragments arranged by size:
2037 1647 455
BsaHI Gr'CG_yC
Cuts at: 1570 1818 2520 4016 1570
Size: 248 702 1496 1693
Fragments arranged by size:
1693 1496 702 248
BsaWI w'CCGG_w
Cuts at: 245 1328 1849 2527 2801 2948 3359 245
Size: 1083 521 678 274 147 411 1025
Fragments arranged by size:
1083 1025 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1041 1071 1041
Size: 30 4109
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 150 860 150
Size: 710 3429
BseYI C'CCAG_C
Cuts at: 203 648 1019 1564 2322 2899 3395 203
Size: 445 371 545 758 577 496 947
Fragments arranged by size:
947 758 577 545 496 445 371
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 748 748
Size: 4139
BsiEI CG_ry'CG
Cuts at: 104 1681 1727 2935 104
Size: 1577 46 1208 1308
Fragments arranged by size:
1577 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 218 267 1153 1931 2121 2913 218
Size: 49 886 778 190 792 1444
Fragments arranged by size:
1444 886 792 778 190 49
Bsp1286I G_dGCh'C
Cuts at: 180 196 218 267 363 374 627 832 1153
Size: 16 22 49 96 11 253 205 321
Cuts at: 1153 1764 1857 1931 2121 2183 2913 180
Size: 611 93 74 190 62 730 1406
Fragments arranged by size:
1406 730 611 321 253 205 190 96
93 74 62 49 22 16 11
BspEI T'CCGG_A
Cuts at: 245 3359 245
Size: 3114 1025
BspHI T'CATG_A
Cuts at: 3315 3315
Size: 4139
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1189 1403 1705 2086 1189
Size: 214 302 381 3242
Fragments arranged by size:
3242 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2431 2485 3969 4134 50
Size: 2381 54 1484 165 55
Fragments arranged by size:
2381 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 148 1040 2051 148
Size: 892 1011 2236
Fragments arranged by size:
2236 1011 892
BsrFI r'CCGG_y
Cuts at: 2137 2318 2527 2137
Size: 181 209 3749
Fragments arranged by size:
3749 209 181
BssSI C'ACGA_G
Cuts at: 514 2410 2768 514
Size: 1896 358 1885
Fragments arranged by size:
1896 1885 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1112 1516 1112
Size: 404 3735
BstBI TT'CG_AA
Cuts at: 2500 2500
Size: 4139
BstXI CCAn_nnnn'nTGG
Cuts at: 502 502
Size: 4139
BstYI r'GATC_y
Cuts at: 1 1164 1631 1654 1989 2235 3247 3333 3345
Size: 1163 467 23 335 246 1012 86 12
Cuts at: 3345 3691 1
Size: 346 449
Fragments arranged by size:
1163 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 601 941 1028 601
Size: 340 87 3712
Fragments arranged by size:
3712 340 87
BtgI C'CryG_G
Cuts at: 107 527 107
Size: 420 3719
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2089 2270 2433 2089
Size: 181 163 3795
Fragments arranged by size:
3795 181 163
BtsI GCAGTG_nn'
Cuts at: 986 1382 986
Size: 396 3743
DraI TTT'AAA
Cuts at: 1138 1138
Size: 4139
DraIII CAC_nnn'GTG
Cuts at: 642 1112 642
Size: 470 3669
DrdI GACnn_nn'nnGTC
Cuts at: 1845 2703 1845
Size: 858 3281
EaeI y'GGCC_r
Cuts at: 225 524 1724 1898 2289 2316 3661 225
Size: 299 1200 174 391 27 1345 703
Fragments arranged by size:
1345 1200 703 391 299 174 27
EagI C'GGCC_G
Cuts at: 1724 1724
Size: 4139
EarI CTCTTCn'nnn_
Cuts at: 290 2162 2372 290
Size: 1872 210 2057
Fragments arranged by size:
2057 1872 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2347 2657 2803 3949 2347
Size: 310 146 1146 2537
Fragments arranged by size:
2537 1146 310 146
EcoICRI GAG'CTC
Cuts at: 216 1151 216
Size: 935 3204
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 192 206 736 1964 2396 3143 3411 192
Size: 14 530 1228 432 747 268 920
Fragments arranged by size:
1228 920 747 530 432 268 14
EcoO109I rG'GnC_Cy
Cuts at: 231 324 847 1278 231
Size: 93 523 431 3092
Fragments arranged by size:
3092 523 431 93
EcoRI G'AATT_C
Cuts at: 1064 1143 1064
Size: 79 4060
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2158 2158
Size: 4139
FspI TGC'GCA
Cuts at: 1920 1920
Size: 4139
HaeII r_GCGC'y
Cuts at: 1573 1821 2843 3822 1573
Size: 248 1022 979 1890
Fragments arranged by size:
1890 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 377 409 531 563 1981 2011 2013 2043 377
Size: 32 122 32 1418 30 2 30 2473
Fragments arranged by size:
2473 1418 122 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1178 1178
Size: 4139
KasI G'GCGC_C
Cuts at: 1569 1817 1569
Size: 248 3891
KpnI G_GTAC'C
Cuts at: 1159 1159
Size: 4139
MluI A'CGCG_T
Cuts at: 3572 3572
Size: 4139
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2810 2994 2810
Size: 184 3955
MscI TGG'CCA
Cuts at: 227 1900 227
Size: 1673 2466
MslI CAynn'nnrTG
Cuts at: 2128 2255 2128
Size: 127 4012
MspA1I CmG'CkG
Cuts at: 529 613 841 972 1250 1564 1924 2937 3182
Size: 84 228 131 278 314 360 1013 245
Cuts at: 3182 529
Size: 1486
Fragments arranged by size:
1486 1013 360 314 278 245 228 131
84
NaeI GCC'GGC
Cuts at: 2320 2320
Size: 4139
NarI GG'CG_CC
Cuts at: 1570 1818 1570
Size: 248 3891
NcoI C'CATG_G
Cuts at: 107 107
Size: 4139
NgoMIV G'CCGG_C
Cuts at: 2318 2318
Size: 4139
NspI r_CATG'y
Cuts at: 510 1192 1692 2599 510
Size: 682 500 907 2050
Fragments arranged by size:
2050 907 682 500
PciI A'CATG_T
Cuts at: 2595 2595
Size: 4139
PflMI CCAn_nnn'nTGG
Cuts at: 975 975
Size: 4139
PmeI GTTT'AAAC
Cuts at: 1138 1138
Size: 4139
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3278 3310 3278
Size: 32 4107
PpuMI rG'GwC_Cy
Cuts at: 231 324 231
Size: 93 4046
PspXI vC'TCGA_Gb
Cuts at: 402 402
Size: 4139
PstI C_TGCA'G
Cuts at: 1186 1871 1186
Size: 685 3454
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4139
PvuII CAG'CTG
Cuts at: 613 972 1564 1924 613
Size: 359 592 360 2828
Fragments arranged by size:
2828 592 360 359
RsrII CG'GwC_CG
Cuts at: 2334 2334
Size: 4139
SacI G_AGCT'C
Cuts at: 218 1153 218
Size: 935 3204
SacII CC_GC'GG
Cuts at: 530 530
Size: 4139
SalI G'TCGA_C
Cuts at: 1176 1176
Size: 4139
SapI GCTCTTCn'nnn_
Cuts at: 290 2162 2372 290
Size: 1872 210 2057
Fragments arranged by size:
2057 1872 210
SbfI CC_TGCA'GG
Cuts at: 1186 1186
Size: 4139
ScaI AGT'ACT
Cuts at: 3656 3656
Size: 4139
SfcI C'TryA_G
Cuts at: 33 1182 1867 2860 3051 33
Size: 1149 685 993 191 1121
Fragments arranged by size:
1149 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1571 1819 1571
Size: 248 3891
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4139
SmaI CCC'GGG
Cuts at: 134 1161 134
Size: 1027 3112
SmlI C'TyrA_G
Cuts at: 402 1294 2209 2701 2963 402
Size: 892 915 492 262 1578
Fragments arranged by size:
1578 915 892 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4139
SphI G_CATG'C
Cuts at: 510 1192 1692 510
Size: 682 500 2957
Fragments arranged by size:
2957 682 500
StuI AGG'CCT
Cuts at: 408 408
Size: 4139
StyI C'CwwG_G
Cuts at: 107 485 1101 1273 1627 107
Size: 378 616 172 354 2619
Fragments arranged by size:
2619 616 378 354 172
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 282 1722 1855 2314 2523 282
Size: 1440 133 459 209 1898
Fragments arranged by size:
1898 1440 459 209 133
TatI w'GTAC_w
Cuts at: 338 3654 338
Size: 3316 823
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1041 1073 3656 3688 1041
Size: 32 2583 32 1492
Fragments arranged by size:
2583 1492 32 32
Tth111I GACn'n_nGTC
Cuts at: 1936 1936
Size: 4139
XbaI T'CTAG_A
Cuts at: 1170 1170
Size: 4139
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1634 1634
Size: 4139
XhoI C'TCGA_G
Cuts at: 402 402
Size: 4139
XmaI C'CCGG_G
Cuts at: 132 1159 132
Size: 1027 3112
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PflMI PmeI PpiI
PpuMI PspXI PstI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI BaeI BbvCI BfrBI BmgBI BmtI Bpu10I
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I ClaI
CspCI EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PfoI
PmlI PshAI PsiI PspOMI PsrI SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XmnI ZraI