Restriction map for
pF1KE0181
(Circular) (Six-base) MAPSORT of: pF1KE0181.seq Check: 2200 from: 1 to: 3920
pF1KE0181 3920 bp
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AccI GT'mk_AC
Cuts at: 788 958 788
Size: 170 3750
Acc65I G'GTAC_C
Cuts at: 936 936
Size: 3920
AclI AA'CG_TT
Cuts at: 3760 3760
Size: 3920
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 536 1745 2177 2924 3192 536
Size: 1209 432 747 268 1264
Fragments arranged by size:
1264 1209 747 432 268
AflIII A'CryG_T
Cuts at: 1272 2376 3353 1272
Size: 1104 977 1839
Fragments arranged by size:
1839 1104 977
AgeI A'CCGG_T
Cuts at: 2308 2308
Size: 3920
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 440 613 440
Size: 173 3747
AlwNI CAG_nnn'CTG
Cuts at: 180 1225 2792 180
Size: 1045 1567 1308
Fragments arranged by size:
1567 1308 1045
ApaI G_GGCC'C
Cuts at: 877 877
Size: 3920
ApaLI G'TGCA_C
Cuts at: 2690 2690
Size: 3920
ApoI r'AATT_y
Cuts at: 924 3273 3529 924
Size: 2349 256 1315
Fragments arranged by size:
2349 1315 256
AscI GG'CGCG_CC
Cuts at: 193 193
Size: 3920
AseI AT'TA_AT
Cuts at: 20 20
Size: 3920
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3920
AvaI C'yCGr_G
Cuts at: 940 940
Size: 3920
BamHI G'GATC_C
Cuts at: 945 945
Size: 3920
BanI G'GyrC_C
Cuts at: 936 1350 1598 1633 936
Size: 414 248 35 3223
Fragments arranged by size:
3223 414 248 35
BanII G_rGCy'C
Cuts at: 398 562 877 934 1964 398
Size: 164 315 57 1030 2354
Fragments arranged by size:
2354 1030 315 164 57
BbeI G_GCGC'C
Cuts at: 1354 1602 1354
Size: 248 3672
BbsI GAAGACnn'nnnn_
Cuts at: 309 309
Size: 3920
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 354 388 354
Size: 34 3886
BciVI GTATCCnnnnn_n'
Cuts at: 1818 2579 3920 1818
Size: 761 1341 1818
Fragments arranged by size:
1818 1341 761
BclI T'GATC_A
Cuts at: 206 1440 206
Size: 1234 2686
BfrBI ATG'CAT
Cuts at: 801 801
Size: 3920
BglII A'GATC_T
Cuts at: 1435 3472 1435
Size: 2037 1883
BlpI GC'TnA_GC
Cuts at: 1032 1032
Size: 3920
Bme1580I G_kGCm'C
Cuts at: 133 673 877 1545 1638 2694 133
Size: 540 204 668 93 1056 1359
Fragments arranged by size:
1359 1056 668 540 204 93
BmrI ACTGGGnnnn_n'
Cuts at: 1132 1213 1548 3692 1132
Size: 81 335 2144 1360
Fragments arranged by size:
2144 1360 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2287 2287
Size: 3920
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 241 241
Size: 3920
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1096 1975 2467 2765 1096
Size: 879 492 298 2251
Fragments arranged by size:
2251 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3624 3624
Size: 3920
BsaAI yAC'GTr
Cuts at: 189 1273 189
Size: 1084 2836
BsaBI GATnn'nnATC
Cuts at: 6 1434 3471 6
Size: 1428 2037 455
Fragments arranged by size:
2037 1428 455
BsaHI Gr'CG_yC
Cuts at: 539 635 1351 1599 2301 3797 539
Size: 96 716 248 702 1496 662
Fragments arranged by size:
1496 716 702 662 248 96
BsaWI w'CCGG_w
Cuts at: 1109 1630 2308 2582 2729 3140 1109
Size: 521 678 274 147 411 1889
Fragments arranged by size:
1889 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 578 608 578
Size: 30 3890
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 494 494
Size: 3920
BseYI C'CCAG_C
Cuts at: 330 1345 2103 2680 3176 330
Size: 1015 758 577 496 1074
Fragments arranged by size:
1074 1015 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 397 397
Size: 3920
BsiEI CG_ry'CG
Cuts at: 104 137 1462 1508 2716 104
Size: 33 1325 46 1208 1308
Fragments arranged by size:
1325 1308 1208 46 33
BsiHKAI G_wGCw'C
Cuts at: 562 934 1712 1902 2694 562
Size: 372 778 190 792 1788
Fragments arranged by size:
1788 792 778 372 190
Bsp1286I G_dGCh'C
Cuts at: 133 398 562 673 877 934 1545 1638 1712
Size: 265 164 111 204 57 611 93 74
Cuts at: 1712 1902 1964 2694 133
Size: 190 62 730 1359
Fragments arranged by size:
1359 730 611 265 204 190 164 111
93 74 62 57
BspEI T'CCGG_A
Cuts at: 3140 3140
Size: 3920
BspHI T'CATG_A
Cuts at: 614 3096 614
Size: 2482 1438
BspMI ACCTGCnnnn'nnnn_
Cuts at: 188 305 970 1184 1486 1867 188
Size: 117 665 214 302 381 2241
Fragments arranged by size:
2241 665 381 302 214 117
BsrBI CCG'CTC
Cuts at: 50 2212 2266 3750 3915 50
Size: 2162 54 1484 165 55
Fragments arranged by size:
2162 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1832 1832
Size: 3920
BsrFI r'CCGG_y
Cuts at: 1918 2099 2308 1918
Size: 181 209 3530
Fragments arranged by size:
3530 209 181
BssHII G'CGCG_C
Cuts at: 193 193
Size: 3920
BssSI C'ACGA_G
Cuts at: 2191 2549 2191
Size: 358 3562
BstAPI GCAn_nnn'nTGC
Cuts at: 180 1297 180
Size: 1117 2803
BstBI TT'CG_AA
Cuts at: 2281 2281
Size: 3920
BstYI r'GATC_y
Cuts at: 1 945 1412 1435 1770 2016 3028 3114 3126
Size: 944 467 23 335 246 1012 86 12
Cuts at: 3126 3472 1
Size: 346 449
Fragments arranged by size:
1012 944 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 890 107
Size: 783 3137
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1870 2051 2214 1870
Size: 181 163 3576
Fragments arranged by size:
3576 181 163
BtsI GCAGTG_nn'
Cuts at: 1163 1163
Size: 3920
DraI TTT'AAA
Cuts at: 705 919 705
Size: 214 3706
DrdI GACnn_nn'nnGTC
Cuts at: 1626 2484 1626
Size: 858 3062
EaeI y'GGCC_r
Cuts at: 134 692 1505 1679 2070 2097 3442 134
Size: 558 813 174 391 27 1345 612
Fragments arranged by size:
1345 813 612 558 391 174 27
EagI C'GGCC_G
Cuts at: 134 1505 134
Size: 1371 2549
EarI CTCTTCn'nnn_
Cuts at: 517 1943 2153 517
Size: 1426 210 2284
Fragments arranged by size:
2284 1426 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2128 2438 2584 3730 2128
Size: 310 146 1146 2318
Fragments arranged by size:
2318 1146 310 146
EcoICRI GAG'CTC
Cuts at: 560 932 560
Size: 372 3548
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 241 536 1745 2177 2924 3192 241
Size: 295 1209 432 747 268 969
Fragments arranged by size:
1209 969 747 432 295 268
EcoO109I rG'GnC_Cy
Cuts at: 874 1059 874
Size: 185 3735
EcoRI G'AATT_C
Cuts at: 924 924
Size: 3920
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1939 1939
Size: 3920
FspI TGC'GCA
Cuts at: 1701 1701
Size: 3920
HaeII r_GCGC'y
Cuts at: 1354 1602 2624 3603 1354
Size: 248 1022 979 1671
Fragments arranged by size:
1671 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 578 610 1762 1792 1794 1824 578
Size: 32 1152 30 2 30 2674
Fragments arranged by size:
2674 1152 32 30 30 2
HincII GTy'rAC
Cuts at: 959 959
Size: 3920
KasI G'GCGC_C
Cuts at: 1350 1598 1350
Size: 248 3672
KpnI G_GTAC'C
Cuts at: 940 940
Size: 3920
MluI A'CGCG_T
Cuts at: 3353 3353
Size: 3920
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2591 2775 2591
Size: 184 3736
MscI TGG'CCA
Cuts at: 694 1681 694
Size: 987 2933
MslI CAynn'nnrTG
Cuts at: 235 838 1909 2036 235
Size: 603 1071 127 2119
Fragments arranged by size:
2119 1071 603 127
MspA1I CmG'CkG
Cuts at: 339 1031 1345 1705 2718 2963 339
Size: 692 314 360 1013 245 1296
Fragments arranged by size:
1296 1013 692 360 314 245
NaeI GCC'GGC
Cuts at: 2101 2101
Size: 3920
NarI GG'CG_CC
Cuts at: 1351 1599 1351
Size: 248 3672
NcoI C'CATG_G
Cuts at: 107 890 107
Size: 783 3137
NgoMIV G'CCGG_C
Cuts at: 2099 2099
Size: 3920
NsiI A_TGCA'T
Cuts at: 803 803
Size: 3920
NspI r_CATG'y
Cuts at: 531 579 973 1473 2380 531
Size: 48 394 500 907 2071
Fragments arranged by size:
2071 907 500 394 48
PasI CC'CwG_GG
Cuts at: 474 870 877 474
Size: 396 7 3517
Fragments arranged by size:
3517 396 7
PciI A'CATG_T
Cuts at: 2376 2376
Size: 3920
PfoI T'CCnGG_A
Cuts at: 218 218
Size: 3920
PmeI GTTT'AAAC
Cuts at: 919 919
Size: 3920
PmlI CAC'GTG
Cuts at: 189 189
Size: 3920
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3059 3091 3059
Size: 32 3888
PspOMI G'GGCC_C
Cuts at: 873 873
Size: 3920
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 277 309 277
Size: 32 3888
PstI C_TGCA'G
Cuts at: 419 967 1652 419
Size: 548 685 2687
Fragments arranged by size:
2687 685 548
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3920
PvuII CAG'CTG
Cuts at: 339 1345 1705 339
Size: 1006 360 2554
Fragments arranged by size:
2554 1006 360
RsrII CG'GwC_CG
Cuts at: 2115 2115
Size: 3920
SacI G_AGCT'C
Cuts at: 562 934 562
Size: 372 3548
SalI G'TCGA_C
Cuts at: 957 957
Size: 3920
SapI GCTCTTCn'nnn_
Cuts at: 517 1943 2153 517
Size: 1426 210 2284
Fragments arranged by size:
2284 1426 210
SbfI CC_TGCA'GG
Cuts at: 967 967
Size: 3920
ScaI AGT'ACT
Cuts at: 3437 3437
Size: 3920
SfcI C'TryA_G
Cuts at: 33 415 429 963 1648 2641 2832 33
Size: 382 14 534 685 993 191 1121
Fragments arranged by size:
1121 993 685 534 382 191 14
SfoI GGC'GCC
Cuts at: 1352 1600 1352
Size: 248 3672
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3920
SmaI CCC'GGG
Cuts at: 942 942
Size: 3920
SmlI C'TyrA_G
Cuts at: 1075 1990 2482 2744 1075
Size: 915 492 262 2251
Fragments arranged by size:
2251 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3920
SphI G_CATG'C
Cuts at: 973 1473 973
Size: 500 3420
StuI AGG'CCT
Cuts at: 485 485
Size: 3920
StyI C'CwwG_G
Cuts at: 107 890 1054 1408 107
Size: 783 164 354 2619
Fragments arranged by size:
2619 783 354 164
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1503 1636 2095 2304 1503
Size: 133 459 209 3119
Fragments arranged by size:
3119 459 209 133
TatI w'GTAC_w
Cuts at: 572 3435 572
Size: 2863 1057
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3437 3469 3437
Size: 32 3888
Tth111I GACn'n_nGTC
Cuts at: 834 1717 834
Size: 883 3037
XbaI T'CTAG_A
Cuts at: 951 951
Size: 3920
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1415 1415
Size: 3920
XmaI C'CCGG_G
Cuts at: 940 940
Size: 3920
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NsiI NspI PasI PciI PfoI
PmeI PmlI PpiI PspOMI PsrI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI AvrII
BaeI BbvCI BglI BmgBI BmtI Bpu10I BsiWI BsmI
BsmBI BsrGI BstEII BstXI BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI PacI PflMI PpuMI PshAI
PsiI PspXI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI XmnI ZraI