Restriction map for
pF1KE0183
(Circular) (Six-base) MAPSORT of: pF1KE0183.seq Check: 2678 from: 1 to: 4079
pF1KE0183 4079 bp
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AccI GT'mk_AC
Cuts at: 1117 1117
Size: 4079
Acc65I G'GTAC_C
Cuts at: 1095 1095
Size: 4079
AclI AA'CG_TT
Cuts at: 3919 3919
Size: 4079
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1904 2336 3083 3351 1904
Size: 432 747 268 2632
Fragments arranged by size:
2632 747 432 268
AflIII A'CryG_T
Cuts at: 1050 1431 2535 3512 1050
Size: 381 1104 977 1617
Fragments arranged by size:
1617 1104 977 381
AgeI A'CCGG_T
Cuts at: 2467 2467
Size: 4079
AleI CACnn'nnGTG
Cuts at: 951 951
Size: 4079
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 629 661 629
Size: 32 4047
AlwNI CAG_nnn'CTG
Cuts at: 823 1384 2951 823
Size: 561 1567 1951
Fragments arranged by size:
1951 1567 561
ApaLI G'TGCA_C
Cuts at: 899 2849 899
Size: 1950 2129
ApoI r'AATT_y
Cuts at: 1083 3432 3688 1083
Size: 2349 256 1474
Fragments arranged by size:
2349 1474 256
AseI AT'TA_AT
Cuts at: 20 680 691 20
Size: 660 11 3408
Fragments arranged by size:
3408 660 11
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4079
AvaI C'yCGr_G
Cuts at: 238 1099 238
Size: 861 3218
BamHI G'GATC_C
Cuts at: 1104 1104
Size: 4079
BanI G'GyrC_C
Cuts at: 577 1095 1509 1757 1792 577
Size: 518 414 248 35 2864
Fragments arranged by size:
2864 518 414 248 35
BanII G_rGCy'C
Cuts at: 1093 2123 1093
Size: 1030 3049
BbeI G_GCGC'C
Cuts at: 1513 1761 1513
Size: 248 3831
BciVI GTATCCnnnnn_n'
Cuts at: 1977 2738 4079 1977
Size: 761 1341 1977
Fragments arranged by size:
1977 1341 761
BclI T'GATC_A
Cuts at: 1599 1599
Size: 4079
BfrBI ATG'CAT
Cuts at: 607 607
Size: 4079
BglII A'GATC_T
Cuts at: 1594 3631 1594
Size: 2037 2042
BlpI GC'TnA_GC
Cuts at: 1191 1191
Size: 4079
Bme1580I G_kGCm'C
Cuts at: 903 963 1704 1797 2853 903
Size: 60 741 93 1056 2129
Fragments arranged by size:
2129 1056 741 93 60
BmrI ACTGGGnnnn_n'
Cuts at: 1291 1372 1707 3851 1291
Size: 81 335 2144 1519
Fragments arranged by size:
2144 1519 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2446 2446
Size: 4079
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 134 134
Size: 4079
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1255 2134 2626 2924 1255
Size: 879 492 298 2410
Fragments arranged by size:
2410 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3783 3783
Size: 4079
BsaAI yAC'GTr
Cuts at: 1432 1432
Size: 4079
BsaBI GATnn'nnATC
Cuts at: 6 1593 3630 6
Size: 1587 2037 455
Fragments arranged by size:
2037 1587 455
BsaHI Gr'CG_yC
Cuts at: 1510 1758 2460 3956 1510
Size: 248 702 1496 1633
Fragments arranged by size:
1633 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1268 1789 2467 2741 2888 3299 1268
Size: 521 678 274 147 411 2048
Fragments arranged by size:
2048 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 994 1024 994
Size: 30 4049
BseYI C'CCAG_C
Cuts at: 1504 2262 2839 3335 1504
Size: 758 577 496 2248
Fragments arranged by size:
2248 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1621 1667 2875 104
Size: 1517 46 1208 1308
Fragments arranged by size:
1517 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 903 1093 1871 2061 2853 903
Size: 190 778 190 792 2129
Fragments arranged by size:
2129 792 778 190 190
BsmI GAATG_Cn'
Cuts at: 719 719
Size: 4079
BsmBI CGTCTCn'nnnn_
Cuts at: 515 515
Size: 4079
Bsp1286I G_dGCh'C
Cuts at: 903 963 1093 1704 1797 1871 2061 2123 2853
Size: 60 130 611 93 74 190 62 730
Cuts at: 2853 903
Size: 2129
Fragments arranged by size:
2129 730 611 190 130 93 74 62
60
BspEI T'CCGG_A
Cuts at: 3299 3299
Size: 4079
BspHI T'CATG_A
Cuts at: 3255 3255
Size: 4079
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1129 1343 1645 2026 1129
Size: 214 302 381 3182
Fragments arranged by size:
3182 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2371 2425 3909 4074 50
Size: 2321 54 1484 165 55
Fragments arranged by size:
2321 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1991 1991
Size: 4079
BsrFI r'CCGG_y
Cuts at: 450 2077 2258 2467 450
Size: 1627 181 209 2062
Fragments arranged by size:
2062 1627 209 181
BssSI C'ACGA_G
Cuts at: 2350 2708 2350
Size: 358 3721
BstAPI GCAn_nnn'nTGC
Cuts at: 1456 1456
Size: 4079
BstBI TT'CG_AA
Cuts at: 2440 2440
Size: 4079
BstYI r'GATC_y
Cuts at: 1 1022 1104 1571 1594 1929 2175 3187 3273
Size: 1021 82 467 23 335 246 1012 86
Cuts at: 3273 3285 3631 1
Size: 12 346 449
Fragments arranged by size:
1021 1012 467 449 346 335 246 86
82 23 12
BtgI C'CryG_G
Cuts at: 889 889
Size: 4079
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2029 2210 2373 2029
Size: 181 163 3735
Fragments arranged by size:
3735 181 163
BtsI GCAGTG_nn'
Cuts at: 475 1322 475
Size: 847 3232
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 498 533 498
Size: 35 4044
DraI TTT'AAA
Cuts at: 1078 1078
Size: 4079
DrdI GACnn_nn'nnGTC
Cuts at: 1785 2643 1785
Size: 858 3221
EaeI y'GGCC_r
Cuts at: 830 1664 1838 2229 2256 3601 830
Size: 834 174 391 27 1345 1308
Fragments arranged by size:
1345 1308 834 391 174 27
EagI C'GGCC_G
Cuts at: 1664 1664
Size: 4079
EarI CTCTTCn'nnn_
Cuts at: 874 2102 2312 874
Size: 1228 210 2641
Fragments arranged by size:
2641 1228 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2287 2597 2743 3889 2287
Size: 310 146 1146 2477
Fragments arranged by size:
2477 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1091 1091
Size: 4079
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 134 1904 2336 3083 3351 134
Size: 1770 432 747 268 862
Fragments arranged by size:
1770 862 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1218 1218
Size: 4079
EcoRI G'AATT_C
Cuts at: 1083 1083
Size: 4079
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 888 2098 888
Size: 1210 2869
FspI TGC'GCA
Cuts at: 1860 1860
Size: 4079
HaeII r_GCGC'y
Cuts at: 1513 1761 2783 3762 1513
Size: 248 1022 979 1830
Fragments arranged by size:
1830 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1921 1951 1953 1983 1921
Size: 30 2 30 4017
Fragments arranged by size:
4017 30 30 2
HincII GTy'rAC
Cuts at: 224 1118 224
Size: 894 3185
HindIII A'AGCT_T
Cuts at: 996 996
Size: 4079
HpaI GTT'AAC
Cuts at: 224 224
Size: 4079
KasI G'GCGC_C
Cuts at: 1509 1757 1509
Size: 248 3831
KpnI G_GTAC'C
Cuts at: 1099 1099
Size: 4079
MluI A'CGCG_T
Cuts at: 1050 3512 1050
Size: 2462 1617
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2750 2934 2750
Size: 184 3895
MscI TGG'CCA
Cuts at: 832 1840 832
Size: 1008 3071
MslI CAynn'nnrTG
Cuts at: 406 951 988 2068 2195 406
Size: 545 37 1080 127 2290
Fragments arranged by size:
2290 1080 545 127 37
MspA1I CmG'CkG
Cuts at: 1190 1504 1864 2877 3122 1190
Size: 314 360 1013 245 2147
Fragments arranged by size:
2147 1013 360 314 245
NaeI GCC'GGC
Cuts at: 452 2260 452
Size: 1808 2271
NarI GG'CG_CC
Cuts at: 1510 1758 1510
Size: 248 3831
NcoI C'CATG_G
Cuts at: 889 889
Size: 4079
NgoMIV G'CCGG_C
Cuts at: 450 2258 450
Size: 1808 2271
NsiI A_TGCA'T
Cuts at: 609 609
Size: 4079
NspI r_CATG'y
Cuts at: 622 1132 1632 2539 622
Size: 510 500 907 2162
Fragments arranged by size:
2162 907 510 500
PciI A'CATG_T
Cuts at: 2535 2535
Size: 4079
PfoI T'CCnGG_A
Cuts at: 802 802
Size: 4079
PmeI GTTT'AAAC
Cuts at: 1078 1078
Size: 4079
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3218 3250 3218
Size: 32 4047
PstI C_TGCA'G
Cuts at: 1126 1811 1126
Size: 685 3394
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4079
PvuII CAG'CTG
Cuts at: 1504 1864 1504
Size: 360 3719
RsrII CG'GwC_CG
Cuts at: 2274 2274
Size: 4079
SacI G_AGCT'C
Cuts at: 1093 1093
Size: 4079
SalI G'TCGA_C
Cuts at: 1116 1116
Size: 4079
SapI GCTCTTCn'nnn_
Cuts at: 2102 2312 2102
Size: 210 3869
SbfI CC_TGCA'GG
Cuts at: 1126 1126
Size: 4079
ScaI AGT'ACT
Cuts at: 3596 3596
Size: 4079
SfcI C'TryA_G
Cuts at: 33 1122 1807 2800 2991 33
Size: 1089 685 993 191 1121
Fragments arranged by size:
1121 1089 993 685 191
SfoI GGC'GCC
Cuts at: 1511 1759 1511
Size: 248 3831
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4079
SmaI CCC'GGG
Cuts at: 1101 1101
Size: 4079
SmlI C'TyrA_G
Cuts at: 238 1234 2149 2641 2903 238
Size: 996 915 492 262 1414
Fragments arranged by size:
1414 996 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4079
SphI G_CATG'C
Cuts at: 1132 1632 1132
Size: 500 3579
SspI AAT'ATT
Cuts at: 684 684
Size: 4079
StyI C'CwwG_G
Cuts at: 889 1213 1567 889
Size: 324 354 3401
Fragments arranged by size:
3401 354 324
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 918 1662 1795 2254 2463 918
Size: 744 133 459 209 2534
Fragments arranged by size:
2534 744 459 209 133
TatI w'GTAC_w
Cuts at: 258 323 1055 3594 258
Size: 65 732 2539 743
Fragments arranged by size:
2539 743 732 65
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3596 3628 3596
Size: 32 4047
Tth111I GACn'n_nGTC
Cuts at: 1876 1876
Size: 4079
XbaI T'CTAG_A
Cuts at: 1110 1110
Size: 4079
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 810 1574 810
Size: 764 3315
XhoI C'TCGA_G
Cuts at: 238 238
Size: 4079
XmaI C'CCGG_G
Cuts at: 1099 1099
Size: 4079
XmnI GAAnn'nnTTC
Cuts at: 883 883
Size: 4079
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BfrBI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NsiI NspI PciI PfoI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI ApaI AscI
AvrII BaeI BbsI BbvCI BcgI BglI BmgBI BmtI
Bpu10I BseRI BsgI BsiWI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI DraIII EcoNI EcoRV FseI FspAI
MfeI NdeI NheI NotI NruI PacI PasI PflMI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI StuI SwaI
ZraI