Restriction map for
pF1KE0184
(Circular) (Six-base) MAPSORT of: pF1KE0184.seq Check: 5166 from: 1 to: 3971
pF1KE0184 3971 bp
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AccI GT'mk_AC
Cuts at: 1009 1009
Size: 3971
Acc65I G'GTAC_C
Cuts at: 987 987
Size: 3971
AclI AA'CG_TT
Cuts at: 3811 3811
Size: 3971
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 726 817 967 1796 2228 2975 3243 726
Size: 91 150 829 432 747 268 1454
Fragments arranged by size:
1454 829 747 432 268 150 91
AflIII A'CryG_T
Cuts at: 452 1323 2427 3404 452
Size: 871 1104 977 1019
Fragments arranged by size:
1104 1019 977 871
AgeI A'CCGG_T
Cuts at: 2359 2359
Size: 3971
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 202 202
Size: 3971
AlwNI CAG_nnn'CTG
Cuts at: 612 629 1276 2843 612
Size: 17 647 1567 1740
Fragments arranged by size:
1740 1567 647 17
ApaLI G'TGCA_C
Cuts at: 805 2741 805
Size: 1936 2035
ApoI r'AATT_y
Cuts at: 380 553 562 975 3324 3580 380
Size: 173 9 413 2349 256 771
Fragments arranged by size:
2349 771 413 256 173 9
AseI AT'TA_AT
Cuts at: 20 20
Size: 3971
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3971
AvaI C'yCGr_G
Cuts at: 227 991 227
Size: 764 3207
BamHI G'GATC_C
Cuts at: 996 996
Size: 3971
BanI G'GyrC_C
Cuts at: 987 1401 1649 1684 987
Size: 414 248 35 3274
Fragments arranged by size:
3274 414 248 35
BanII G_rGCy'C
Cuts at: 729 985 2015 729
Size: 256 1030 2685
Fragments arranged by size:
2685 1030 256
BbeI G_GCGC'C
Cuts at: 1405 1653 1405
Size: 248 3723
BbsI GAAGACnn'nnnn_
Cuts at: 239 851 955 239
Size: 612 104 3255
Fragments arranged by size:
3255 612 104
BciVI GTATCCnnnnn_n'
Cuts at: 1869 2630 3971 1869
Size: 761 1341 1869
Fragments arranged by size:
1869 1341 761
BclI T'GATC_A
Cuts at: 1491 1491
Size: 3971
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 3971
BglII A'GATC_T
Cuts at: 223 1486 3523 223
Size: 1263 2037 671
Fragments arranged by size:
2037 1263 671
BlpI GC'TnA_GC
Cuts at: 1083 1083
Size: 3971
Bme1580I G_kGCm'C
Cuts at: 809 1596 1689 2745 809
Size: 787 93 1056 2035
Fragments arranged by size:
2035 1056 787 93
BmrI ACTGGGnnnn_n'
Cuts at: 732 1183 1264 1599 3743 732
Size: 451 81 335 2144 960
Fragments arranged by size:
2144 960 451 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2338 2338
Size: 3971
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 510 579 946 510
Size: 69 367 3535
Fragments arranged by size:
3535 367 69
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1147 2026 2518 2816 1147
Size: 879 492 298 2302
Fragments arranged by size:
2302 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3675 3675
Size: 3971
BsaAI yAC'GTr
Cuts at: 455 1324 455
Size: 869 3102
BsaBI GATnn'nnATC
Cuts at: 6 1485 3522 6
Size: 1479 2037 455
Fragments arranged by size:
2037 1479 455
BsaHI Gr'CG_yC
Cuts at: 1402 1650 2352 3848 1402
Size: 248 702 1496 1525
Fragments arranged by size:
1525 1496 702 248
BsaWI w'CCGG_w
Cuts at: 197 260 354 652 1160 1681 2359 2633 2780
Size: 63 94 298 508 521 678 274 147
Cuts at: 2780 3191 197
Size: 411 977
Fragments arranged by size:
977 678 521 508 411 298 274 147
94 63
BseYI C'CCAG_C
Cuts at: 878 1396 2154 2731 3227 878
Size: 518 758 577 496 1622
Fragments arranged by size:
1622 758 577 518 496
BsiEI CG_ry'CG
Cuts at: 104 1513 1559 2767 104
Size: 1409 46 1208 1308
Fragments arranged by size:
1409 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 809 985 1763 1953 2745 809
Size: 176 778 190 792 2035
Fragments arranged by size:
2035 792 778 190 176
BsmI GAATG_Cn'
Cuts at: 364 861 364
Size: 497 3474
Bsp1286I G_dGCh'C
Cuts at: 729 809 985 1596 1689 1763 1953 2015 2745
Size: 80 176 611 93 74 190 62 730
Cuts at: 2745 729
Size: 1955
Fragments arranged by size:
1955 730 611 190 176 93 80 74
62
BspEI T'CCGG_A
Cuts at: 197 260 652 3191 197
Size: 63 392 2539 977
Fragments arranged by size:
2539 977 392 63
BspHI T'CATG_A
Cuts at: 527 935 3147 527
Size: 408 2212 1351
Fragments arranged by size:
2212 1351 408
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1021 1235 1537 1918 1021
Size: 214 302 381 3074
Fragments arranged by size:
3074 381 302 214
BsrBI CCG'CTC
Cuts at: 50 139 2263 2317 3801 3966 50
Size: 89 2124 54 1484 165 55
Fragments arranged by size:
2124 1484 165 89 55 54
BsrDI GCAATG_nn'
Cuts at: 1883 1883
Size: 3971
BsrFI r'CCGG_y
Cuts at: 148 1969 2150 2359 148
Size: 1821 181 209 1760
Fragments arranged by size:
1821 1760 209 181
BsrGI T'GTAC_A
Cuts at: 797 797
Size: 3971
BssHII G'CGCG_C
Cuts at: 126 126
Size: 3971
BssSI C'ACGA_G
Cuts at: 728 2242 2600 728
Size: 1514 358 2099
Fragments arranged by size:
2099 1514 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1348 1348
Size: 3971
BstBI TT'CG_AA
Cuts at: 2332 2332
Size: 3971
BstYI r'GATC_y
Cuts at: 1 223 649 828 996 1463 1486 1821 2067
Size: 222 426 179 168 467 23 335 246
Cuts at: 2067 3079 3165 3177 3523 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 467 449 426 346 335 246 222
179 168 86 23 12
BtgI C'CryG_G
Cuts at: 107 132 107
Size: 25 3946
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1921 2102 2265 1921
Size: 181 163 3627
Fragments arranged by size:
3627 181 163
BtsI GCAGTG_nn'
Cuts at: 1214 1214
Size: 3971
DraI TTT'AAA
Cuts at: 970 970
Size: 3971
DrdI GACnn_nn'nnGTC
Cuts at: 478 1677 2535 478
Size: 1199 858 1914
Fragments arranged by size:
1914 1199 858
EaeI y'GGCC_r
Cuts at: 1556 1730 2121 2148 3493 1556
Size: 174 391 27 1345 2034
Fragments arranged by size:
2034 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1556 1556
Size: 3971
EarI CTCTTCn'nnn_
Cuts at: 1994 2204 1994
Size: 210 3761
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2179 2489 2635 3781 2179
Size: 310 146 1146 2369
Fragments arranged by size:
2369 1146 310 146
EcoICRI GAG'CTC
Cuts at: 983 983
Size: 3971
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 510 579 726 817 946 967 1796 2228 2975
Size: 69 147 91 129 21 829 432 747
Cuts at: 2975 3243 510
Size: 268 1238
Fragments arranged by size:
1238 829 747 432 268 147 129 91
69 21
EcoO109I rG'GnC_Cy
Cuts at: 1110 1110
Size: 3971
EcoRI G'AATT_C
Cuts at: 975 975
Size: 3971
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1990 1990
Size: 3971
FspI TGC'GCA
Cuts at: 1752 1752
Size: 3971
HaeII r_GCGC'y
Cuts at: 1405 1653 2675 3654 1405
Size: 248 1022 979 1722
Fragments arranged by size:
1722 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 274 306 1813 1843 1845 1875 274
Size: 32 1507 30 2 30 2370
Fragments arranged by size:
2370 1507 32 30 30 2
HincII GTy'rAC
Cuts at: 1010 1010
Size: 3971
KasI G'GCGC_C
Cuts at: 1401 1649 1401
Size: 248 3723
KpnI G_GTAC'C
Cuts at: 991 991
Size: 3971
MluI A'CGCG_T
Cuts at: 3404 3404
Size: 3971
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 596 714 747 2642 2826 596
Size: 118 33 1895 184 1741
Fragments arranged by size:
1895 1741 184 118 33
MscI TGG'CCA
Cuts at: 1732 1732
Size: 3971
MslI CAynn'nnrTG
Cuts at: 872 1960 2087 872
Size: 1088 127 2756
Fragments arranged by size:
2756 1088 127
MspA1I CmG'CkG
Cuts at: 134 165 239 795 1082 1396 1756 2769 3014
Size: 31 74 556 287 314 360 1013 245
Cuts at: 3014 134
Size: 1091
Fragments arranged by size:
1091 1013 556 360 314 287 245 74
31
NaeI GCC'GGC
Cuts at: 150 2152 150
Size: 2002 1969
NarI GG'CG_CC
Cuts at: 1402 1650 1402
Size: 248 3723
NcoI C'CATG_G
Cuts at: 107 107
Size: 3971
NgoMIV G'CCGG_C
Cuts at: 148 2150 148
Size: 2002 1969
NspI r_CATG'y
Cuts at: 588 1024 1524 2431 588
Size: 436 500 907 2128
Fragments arranged by size:
2128 907 500 436
PciI A'CATG_T
Cuts at: 2427 2427
Size: 3971
PfoI T'CCnGG_A
Cuts at: 595 595
Size: 3971
PmeI GTTT'AAAC
Cuts at: 970 970
Size: 3971
PmlI CAC'GTG
Cuts at: 455 455
Size: 3971
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3110 3142 3110
Size: 32 3939
PstI C_TGCA'G
Cuts at: 164 181 1018 1703 164
Size: 17 837 685 2432
Fragments arranged by size:
2432 837 685 17
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3971
PvuII CAG'CTG
Cuts at: 239 795 1396 1756 239
Size: 556 601 360 2454
Fragments arranged by size:
2454 601 556 360
RsrII CG'GwC_CG
Cuts at: 2166 2166
Size: 3971
SacI G_AGCT'C
Cuts at: 985 985
Size: 3971
SacII CC_GC'GG
Cuts at: 135 135
Size: 3971
SalI G'TCGA_C
Cuts at: 1008 1008
Size: 3971
SapI GCTCTTCn'nnn_
Cuts at: 1994 2204 1994
Size: 210 3761
SbfI CC_TGCA'GG
Cuts at: 1018 1018
Size: 3971
ScaI AGT'ACT
Cuts at: 3488 3488
Size: 3971
SfcI C'TryA_G
Cuts at: 33 160 177 1014 1699 2692 2883 33
Size: 127 17 837 685 993 191 1121
Fragments arranged by size:
1121 993 837 685 191 127 17
SfoI GGC'GCC
Cuts at: 1403 1651 1403
Size: 248 3723
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3971
SmaI CCC'GGG
Cuts at: 993 993
Size: 3971
SmlI C'TyrA_G
Cuts at: 227 1126 2041 2533 2795 227
Size: 899 915 492 262 1403
Fragments arranged by size:
1403 915 899 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3971
SphI G_CATG'C
Cuts at: 1024 1524 1024
Size: 500 3471
StyI C'CwwG_G
Cuts at: 107 1105 1459 107
Size: 998 354 2619
Fragments arranged by size:
2619 998 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1554 1687 2146 2355 1554
Size: 133 459 209 3170
Fragments arranged by size:
3170 459 209 133
TatI w'GTAC_w
Cuts at: 430 797 3486 430
Size: 367 2689 915
Fragments arranged by size:
2689 915 367
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3488 3520 3488
Size: 32 3939
Tth111I GACn'n_nGTC
Cuts at: 1768 1768
Size: 3971
XbaI T'CTAG_A
Cuts at: 1002 1002
Size: 3971
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1466 1466
Size: 3971
XhoI C'TCGA_G
Cuts at: 227 227
Size: 3971
XmaI C'CCGG_G
Cuts at: 991 991
Size: 3971
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PfoI PmeI PmlI PpiI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI
Bpu10I BsaXI BseRI BsgI BsiWI BsmBI BstEII BstXI
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PpuMI PshAI PsiI PspOMI
PspXI PsrI SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XmnI ZraI