Restriction map for
pF1KE0187
(Circular) (Six-base) MAPSORT of: pF1KE0187.seq Check: 1377 from: 1 to: 3845
pF1KE0187 3845 bp
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AccI GT'mk_AC
Cuts at: 691 883 691
Size: 192 3653
Acc65I G'GTAC_C
Cuts at: 861 861
Size: 3845
AclI AA'CG_TT
Cuts at: 3685 3685
Size: 3845
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 341 1670 2102 2849 3117 341
Size: 1329 432 747 268 1069
Fragments arranged by size:
1329 1069 747 432 268
AflIII A'CryG_T
Cuts at: 1197 2301 3278 1197
Size: 1104 977 1764
Fragments arranged by size:
1764 1104 977
AgeI A'CCGG_T
Cuts at: 2233 2233
Size: 3845
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 531 563 531
Size: 32 3813
AlwNI CAG_nnn'CTG
Cuts at: 1150 2717 1150
Size: 1567 2278
ApaI G_GGCC'C
Cuts at: 124 124
Size: 3845
ApaLI G'TGCA_C
Cuts at: 2615 2615
Size: 3845
ApoI r'AATT_y
Cuts at: 760 849 3198 3454 760
Size: 89 2349 256 1151
Fragments arranged by size:
2349 1151 256 89
AseI AT'TA_AT
Cuts at: 20 20
Size: 3845
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3845
AvaI C'yCGr_G
Cuts at: 865 865
Size: 3845
BamHI G'GATC_C
Cuts at: 870 870
Size: 3845
BanI G'GyrC_C
Cuts at: 861 1275 1523 1558 861
Size: 414 248 35 3148
Fragments arranged by size:
3148 414 248 35
BanII G_rGCy'C
Cuts at: 124 859 1889 124
Size: 735 1030 2080
Fragments arranged by size:
2080 1030 735
BbeI G_GCGC'C
Cuts at: 1279 1527 1279
Size: 248 3597
BbvCI CC'TCA_GC
Cuts at: 171 171
Size: 3845
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3811
BciVI GTATCCnnnnn_n'
Cuts at: 1743 2504 3845 1743
Size: 761 1341 1743
Fragments arranged by size:
1743 1341 761
BclI T'GATC_A
Cuts at: 1365 1365
Size: 3845
BglII A'GATC_T
Cuts at: 186 1360 3397 186
Size: 1174 2037 634
Fragments arranged by size:
2037 1174 634
BlpI GC'TnA_GC
Cuts at: 957 957
Size: 3845
Bme1580I G_kGCm'C
Cuts at: 124 1470 1563 2619 124
Size: 1346 93 1056 1350
Fragments arranged by size:
1350 1346 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1057 1138 1473 3617 1057
Size: 81 335 2144 1285
Fragments arranged by size:
2144 1285 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2212 2212
Size: 3845
Bpu10I CC'TnA_GC
Cuts at: 171 171
Size: 3845
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1021 1900 2392 2690 1021
Size: 879 492 298 2176
Fragments arranged by size:
2176 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 522 3549 522
Size: 3027 818
BsaAI yAC'GTr
Cuts at: 1198 1198
Size: 3845
BsaBI GATnn'nnATC
Cuts at: 6 314 1359 3396 6
Size: 308 1045 2037 455
Fragments arranged by size:
2037 1045 455 308
BsaHI Gr'CG_yC
Cuts at: 1276 1524 2226 3722 1276
Size: 248 702 1496 1399
Fragments arranged by size:
1496 1399 702 248
BsaWI w'CCGG_w
Cuts at: 1034 1555 2233 2507 2654 3065 1034
Size: 521 678 274 147 411 1814
Fragments arranged by size:
1814 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 159 159
Size: 3845
BseYI C'CCAG_C
Cuts at: 1270 2028 2605 3101 1270
Size: 758 577 496 2014
Fragments arranged by size:
2014 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1387 1433 2641 104
Size: 1283 46 1208 1308
Fragments arranged by size:
1308 1283 1208 46
BsiHKAI G_wGCw'C
Cuts at: 859 1637 1827 2619 859
Size: 778 190 792 2085
Fragments arranged by size:
2085 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 124 859 1470 1563 1637 1827 1889 2619 124
Size: 735 611 93 74 190 62 730 1350
Fragments arranged by size:
1350 735 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3065 3065
Size: 3845
BspHI T'CATG_A
Cuts at: 3021 3021
Size: 3845
BspMI ACCTGCnnnn'nnnn_
Cuts at: 895 1109 1411 1792 895
Size: 214 302 381 2948
Fragments arranged by size:
2948 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2137 2191 3675 3840 50
Size: 2087 54 1484 165 55
Fragments arranged by size:
2087 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1757 1757
Size: 3845
BsrFI r'CCGG_y
Cuts at: 1843 2024 2233 1843
Size: 181 209 3455
Fragments arranged by size:
3455 209 181
BssSI C'ACGA_G
Cuts at: 2116 2474 2116
Size: 358 3487
BstAPI GCAn_nnn'nTGC
Cuts at: 1222 1222
Size: 3845
BstBI TT'CG_AA
Cuts at: 2206 2206
Size: 3845
BstXI CCAn_nnnn'nTGG
Cuts at: 610 610
Size: 3845
BstYI r'GATC_y
Cuts at: 1 186 870 1337 1360 1695 1941 2953 3039
Size: 185 684 467 23 335 246 1012 86
Cuts at: 3039 3051 3397 1
Size: 12 346 449
Fragments arranged by size:
1012 684 467 449 346 335 246 185
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1795 1976 2139 1795
Size: 181 163 3501
Fragments arranged by size:
3501 181 163
BtsI GCAGTG_nn'
Cuts at: 1088 1088
Size: 3845
DraI TTT'AAA
Cuts at: 844 844
Size: 3845
DrdI GACnn_nn'nnGTC
Cuts at: 1551 2409 1551
Size: 858 2987
EaeI y'GGCC_r
Cuts at: 569 600 1430 1604 1995 2022 3367 569
Size: 31 830 174 391 27 1345 1047
Fragments arranged by size:
1345 1047 830 391 174 31 27
EagI C'GGCC_G
Cuts at: 1430 1430
Size: 3845
EarI CTCTTCn'nnn_
Cuts at: 785 1868 2078 785
Size: 1083 210 2552
Fragments arranged by size:
2552 1083 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2053 2363 2509 3655 2053
Size: 310 146 1146 2243
Fragments arranged by size:
2243 1146 310 146
EcoICRI GAG'CTC
Cuts at: 857 857
Size: 3845
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 341 1670 2102 2849 3117 341
Size: 1329 432 747 268 1069
Fragments arranged by size:
1329 1069 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 457 984 457
Size: 527 3318
EcoRI G'AATT_C
Cuts at: 760 849 760
Size: 89 3756
EcoRV GAT'ATC
Cuts at: 491 491
Size: 3845
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 354 1864 354
Size: 1510 2335
FspI TGC'GCA
Cuts at: 1626 1626
Size: 3845
HaeII r_GCGC'y
Cuts at: 1279 1527 2549 3528 1279
Size: 248 1022 979 1596
Fragments arranged by size:
1596 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 227 259 426 458 1687 1717 1719 1749 227
Size: 32 167 32 1229 30 2 30 2323
Fragments arranged by size:
2323 1229 167 32 32 30 30 2
HincII GTy'rAC
Cuts at: 884 884
Size: 3845
KasI G'GCGC_C
Cuts at: 1275 1523 1275
Size: 248 3597
KpnI G_GTAC'C
Cuts at: 865 865
Size: 3845
MluI A'CGCG_T
Cuts at: 3278 3278
Size: 3845
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 651 2516 2700 651
Size: 1865 184 1796
Fragments arranged by size:
1865 1796 184
MscI TGG'CCA
Cuts at: 571 602 1606 571
Size: 31 1004 2810
Fragments arranged by size:
2810 1004 31
MslI CAynn'nnrTG
Cuts at: 1834 1961 1834
Size: 127 3718
MspA1I CmG'CkG
Cuts at: 956 1270 1630 2643 2888 956
Size: 314 360 1013 245 1913
Fragments arranged by size:
1913 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2026 2026
Size: 3845
NarI GG'CG_CC
Cuts at: 1276 1524 1276
Size: 248 3597
NdeI CA'TA_TG
Cuts at: 597 597
Size: 3845
NgoMIV G'CCGG_C
Cuts at: 2024 2024
Size: 3845
NspI r_CATG'y
Cuts at: 898 1398 2305 898
Size: 500 907 2438
Fragments arranged by size:
2438 907 500
PciI A'CATG_T
Cuts at: 2301 2301
Size: 3845
PflMI CCAn_nnn'nTGG
Cuts at: 412 412
Size: 3845
PmeI GTTT'AAAC
Cuts at: 844 844
Size: 3845
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2984 3016 2984
Size: 32 3813
PpuMI rG'GwC_Cy
Cuts at: 457 457
Size: 3845
PsiI TTA'TAA
Cuts at: 529 700 529
Size: 171 3674
PspOMI G'GGCC_C
Cuts at: 120 120
Size: 3845
PstI C_TGCA'G
Cuts at: 892 1577 892
Size: 685 3160
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3845
PvuII CAG'CTG
Cuts at: 1270 1630 1270
Size: 360 3485
RsrII CG'GwC_CG
Cuts at: 2040 2040
Size: 3845
SacI G_AGCT'C
Cuts at: 859 859
Size: 3845
SalI G'TCGA_C
Cuts at: 882 882
Size: 3845
SapI GCTCTTCn'nnn_
Cuts at: 1868 2078 1868
Size: 210 3635
SbfI CC_TGCA'GG
Cuts at: 892 892
Size: 3845
ScaI AGT'ACT
Cuts at: 3362 3362
Size: 3845
SfcI C'TryA_G
Cuts at: 33 254 729 888 1573 2566 2757 33
Size: 221 475 159 685 993 191 1121
Fragments arranged by size:
1121 993 685 475 221 191 159
SfoI GGC'GCC
Cuts at: 1277 1525 1277
Size: 248 3597
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3845
SmaI CCC'GGG
Cuts at: 867 867
Size: 3845
SmlI C'TyrA_G
Cuts at: 1000 1915 2407 2669 1000
Size: 915 492 262 2176
Fragments arranged by size:
2176 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3845
SphI G_CATG'C
Cuts at: 898 1398 898
Size: 500 3345
StyI C'CwwG_G
Cuts at: 609 979 1333 609
Size: 370 354 3121
Fragments arranged by size:
3121 370 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 742 1428 1561 2020 2229 742
Size: 686 133 459 209 2358
Fragments arranged by size:
2358 686 459 209 133
TatI w'GTAC_w
Cuts at: 657 3360 657
Size: 2703 1142
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3362 3394 3362
Size: 32 3813
Tth111I GACn'n_nGTC
Cuts at: 1642 1642
Size: 3845
XbaI T'CTAG_A
Cuts at: 876 876
Size: 3845
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1340 1340
Size: 3845
XmaI C'CCGG_G
Cuts at: 865 865
Size: 3845
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbvCI BcgI BciVI BclI BglII BlpI
Bme1580I BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NdeI NgoMIV NspI
PciI PflMI PmeI PpiI PpuMI PsiI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AscI
AvrII BaeI BbsI BfrBI BglI BmgBI BmtI BpmI
BsaXI BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstZ17I Bsu36I BtgI ClaI CspCI DraIII EcoNI FseI
FspAI HindIII HpaI MfeI NcoI NheI NotI NruI
NsiI PacI PasI PfoI PmlI PshAI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI