Restriction map for
pF1KE0195
(Circular) (Six-base) MAPSORT of: pF1KE0195.seq Check: 6050 from: 1 to: 4343
pF1KE0195 4343 bp
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1204 1204
Size: 4343
AccI GT'mk_AC
Cuts at: 1296 1381 1296
Size: 85 4258
Acc65I G'GTAC_C
Cuts at: 1359 1359
Size: 4343
AclI AA'CG_TT
Cuts at: 4183 4183
Size: 4343
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 814 2168 2600 3347 3615 814
Size: 1354 432 747 268 1542
Fragments arranged by size:
1542 1354 747 432 268
AflIII A'CryG_T
Cuts at: 1695 2799 3776 1695
Size: 1104 977 2262
Fragments arranged by size:
2262 1104 977
AgeI A'CCGG_T
Cuts at: 2731 2731
Size: 4343
AleI CACnn'nnGTG
Cuts at: 1188 1188
Size: 4343
AlwNI CAG_nnn'CTG
Cuts at: 474 1648 3215 474
Size: 1174 1567 1602
Fragments arranged by size:
1602 1567 1174
ApaLI G'TGCA_C
Cuts at: 1191 3113 1191
Size: 1922 2421
ApoI r'AATT_y
Cuts at: 1347 3696 3952 1347
Size: 2349 256 1738
Fragments arranged by size:
2349 1738 256
AscI GG'CGCG_CC
Cuts at: 1248 1248
Size: 4343
AseI AT'TA_AT
Cuts at: 20 20
Size: 4343
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4343
AvaI C'yCGr_G
Cuts at: 493 574 1363 493
Size: 81 789 3473
Fragments arranged by size:
3473 789 81
BamHI G'GATC_C
Cuts at: 1368 1368
Size: 4343
BanI G'GyrC_C
Cuts at: 263 362 369 1359 1773 2021 2056 263
Size: 99 7 990 414 248 35 2550
Fragments arranged by size:
2550 990 414 248 99 35 7
BanII G_rGCy'C
Cuts at: 139 572 1357 2387 139
Size: 433 785 1030 2095
Fragments arranged by size:
2095 1030 785 433
BbeI G_GCGC'C
Cuts at: 1777 2025 1777
Size: 248 4095
BbsI GAAGACnn'nnnn_
Cuts at: 200 596 200
Size: 396 3947
BciVI GTATCCnnnnn_n'
Cuts at: 357 2241 3002 4343 357
Size: 1884 761 1341 357
Fragments arranged by size:
1884 1341 761 357
BclI T'GATC_A
Cuts at: 1175 1863 1175
Size: 688 3655
BglI GCCn_nnn'nGGC
Cuts at: 206 206
Size: 4343
BglII A'GATC_T
Cuts at: 1858 3895 1858
Size: 2037 2306
BlpI GC'TnA_GC
Cuts at: 1455 1455
Size: 4343
Bme1580I G_kGCm'C
Cuts at: 1195 1968 2061 3117 1195
Size: 773 93 1056 2421
Fragments arranged by size:
2421 1056 773 93
BmgBI CAC'GTC
Cuts at: 668 668
Size: 4343
BmrI ACTGGGnnnn_n'
Cuts at: 197 539 1555 1636 1971 4115 197
Size: 342 1016 81 335 2144 425
Fragments arranged by size:
2144 1016 425 342 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2710 2710
Size: 4343
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 451 496 748 841 1042 1069 1272 1307 451
Size: 45 252 93 201 27 203 35 3487
Fragments arranged by size:
3487 252 203 201 93 45 35 27
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 322 1519 2398 2890 3188 322
Size: 1197 879 492 298 1477
Fragments arranged by size:
1477 1197 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 800 988 4047 800
Size: 188 3059 1096
Fragments arranged by size:
3059 1096 188
BsaAI yAC'GTr
Cuts at: 1696 1696
Size: 4343
BsaBI GATnn'nnATC
Cuts at: 6 1213 1857 3894 6
Size: 1207 644 2037 455
Fragments arranged by size:
2037 1207 644 455
BsaHI Gr'CG_yC
Cuts at: 1774 2022 2724 4220 1774
Size: 248 702 1496 1897
Fragments arranged by size:
1897 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1532 2053 2731 3005 3152 3563 1532
Size: 521 678 274 147 411 2312
Fragments arranged by size:
2312 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 826 856 826
Size: 30 4313
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 850 850
Size: 4343
BseYI C'CCAG_C
Cuts at: 139 525 692 1768 2526 3103 3599 139
Size: 386 167 1076 758 577 496 883
Fragments arranged by size:
1076 883 758 577 496 386 167
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1182 1182
Size: 4343
BsiEI CG_ry'CG
Cuts at: 104 1885 1931 3139 104
Size: 1781 46 1208 1308
Fragments arranged by size:
1781 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 440 1064 1195 1357 2135 2325 3117 440
Size: 624 131 162 778 190 792 1666
Fragments arranged by size:
1666 792 778 624 190 162 131
Bsp1286I G_dGCh'C
Cuts at: 139 440 572 1064 1195 1357 1968 2061 2135
Size: 301 132 492 131 162 611 93 74
Cuts at: 2135 2325 2387 3117 139
Size: 190 62 730 1365
Fragments arranged by size:
1365 730 611 492 301 190 162 132
131 93 74 62
BspEI T'CCGG_A
Cuts at: 3563 3563
Size: 4343
BspHI T'CATG_A
Cuts at: 3519 3519
Size: 4343
BspMI ACCTGCnnnn'nnnn_
Cuts at: 614 713 815 1204 1393 1607 1909 2290 614
Size: 99 102 389 189 214 302 381 2667
Fragments arranged by size:
2667 389 381 302 214 189 102 99
BsrBI CCG'CTC
Cuts at: 50 2635 2689 4173 4338 50
Size: 2585 54 1484 165 55
Fragments arranged by size:
2585 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2255 2255
Size: 4343
BsrFI r'CCGG_y
Cuts at: 173 359 597 2341 2522 2731 173
Size: 186 238 1744 181 209 1785
Fragments arranged by size:
1785 1744 238 209 186 181
BssHII G'CGCG_C
Cuts at: 408 1248 408
Size: 840 3503
BssSI C'ACGA_G
Cuts at: 2614 2972 2614
Size: 358 3985
BstAPI GCAn_nnn'nTGC
Cuts at: 369 816 1720 369
Size: 447 904 2992
Fragments arranged by size:
2992 904 447
BstBI TT'CG_AA
Cuts at: 2704 2704
Size: 4343
BstYI r'GATC_y
Cuts at: 1 1368 1835 1858 2193 2439 3451 3537 3549
Size: 1367 467 23 335 246 1012 86 12
Cuts at: 3549 3895 1
Size: 346 449
Fragments arranged by size:
1367 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4343
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 408 2293 2474 2637 408
Size: 1885 181 163 2114
Fragments arranged by size:
2114 1885 181 163
BtsI GCAGTG_nn'
Cuts at: 1586 1586
Size: 4343
DraI TTT'AAA
Cuts at: 116 1342 116
Size: 1226 3117
DraIII CAC_nnn'GTG
Cuts at: 369 547 1154 369
Size: 178 607 3558
Fragments arranged by size:
3558 607 178
DrdI GACnn_nn'nnGTC
Cuts at: 598 2049 2907 598
Size: 1451 858 2034
Fragments arranged by size:
2034 1451 858
EaeI y'GGCC_r
Cuts at: 1928 2102 2493 2520 3865 1928
Size: 174 391 27 1345 2406
Fragments arranged by size:
2406 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1928 1928
Size: 4343
EarI CTCTTCn'nnn_
Cuts at: 932 2366 2576 932
Size: 1434 210 2699
Fragments arranged by size:
2699 1434 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 136 2551 2861 3007 4153 136
Size: 2415 310 146 1146 326
Fragments arranged by size:
2415 1146 326 310 146
EcoICRI GAG'CTC
Cuts at: 1355 1355
Size: 4343
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 451 496 748 814 841 1042 1069 1272 1307
Size: 45 252 66 27 201 27 203 35
Cuts at: 1307 2168 2600 3347 3615 451
Size: 861 432 747 268 1179
Fragments arranged by size:
1179 861 747 432 268 252 203 201
66 45 35 27 27
EcoNI CCTnn'n_nnAGG
Cuts at: 832 1239 832
Size: 407 3936
EcoO109I rG'GnC_Cy
Cuts at: 555 623 1227 1281 1482 555
Size: 68 604 54 201 3416
Fragments arranged by size:
3416 604 201 68 54
EcoRI G'AATT_C
Cuts at: 1347 1347
Size: 4343
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2362 2362
Size: 4343
FspI TGC'GCA
Cuts at: 2124 2124
Size: 4343
HaeII r_GCGC'y
Cuts at: 1777 2025 3047 4026 1777
Size: 248 1022 979 2094
Fragments arranged by size:
2094 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2185 2215 2217 2247 2185
Size: 30 2 30 4281
Fragments arranged by size:
4281 30 30 2
HincII GTy'rAC
Cuts at: 603 1382 603
Size: 779 3564
KasI G'GCGC_C
Cuts at: 1773 2021 1773
Size: 248 4095
KpnI G_GTAC'C
Cuts at: 1363 1363
Size: 4343
MluI A'CGCG_T
Cuts at: 3776 3776
Size: 4343
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3014 3198 3014
Size: 184 4159
MscI TGG'CCA
Cuts at: 2104 2104
Size: 4343
MslI CAynn'nnrTG
Cuts at: 1188 1207 2332 2459 1188
Size: 19 1125 127 3072
Fragments arranged by size:
3072 1125 127 19
MspA1I CmG'CkG
Cuts at: 291 813 894 1057 1454 1768 2128 3141 3386
Size: 522 81 163 397 314 360 1013 245
Cuts at: 3386 291
Size: 1248
Fragments arranged by size:
1248 1013 522 397 360 314 245 163
81
NaeI GCC'GGC
Cuts at: 2524 2524
Size: 4343
NarI GG'CG_CC
Cuts at: 1774 2022 1774
Size: 248 4095
NcoI C'CATG_G
Cuts at: 107 107
Size: 4343
NgoMIV G'CCGG_C
Cuts at: 2522 2522
Size: 4343
NspI r_CATG'y
Cuts at: 1396 1896 2803 1396
Size: 500 907 2936
Fragments arranged by size:
2936 907 500
PciI A'CATG_T
Cuts at: 2799 2799
Size: 4343
PflMI CCAn_nnn'nTGG
Cuts at: 474 1268 1330 474
Size: 794 62 3487
Fragments arranged by size:
3487 794 62
PfoI T'CCnGG_A
Cuts at: 467 764 467
Size: 297 4046
PmeI GTTT'AAAC
Cuts at: 1342 1342
Size: 4343
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3482 3514 3482
Size: 32 4311
PpuMI rG'GwC_Cy
Cuts at: 1227 1281 1227
Size: 54 4289
PstI C_TGCA'G
Cuts at: 490 812 887 934 1390 2075 490
Size: 322 75 47 456 685 2758
Fragments arranged by size:
2758 685 456 322 75 47
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4343
PvuII CAG'CTG
Cuts at: 291 813 894 1768 2128 291
Size: 522 81 874 360 2506
Fragments arranged by size:
2506 874 522 360 81
RsrII CG'GwC_CG
Cuts at: 2538 2538
Size: 4343
SacI G_AGCT'C
Cuts at: 1357 1357
Size: 4343
SalI G'TCGA_C
Cuts at: 1380 1380
Size: 4343
SanDI GG'GwC_CC
Cuts at: 1281 1281
Size: 4343
SapI GCTCTTCn'nnn_
Cuts at: 2366 2576 2366
Size: 210 4133
SbfI CC_TGCA'GG
Cuts at: 887 1390 887
Size: 503 3840
ScaI AGT'ACT
Cuts at: 3860 3860
Size: 4343
SexAI A'CCwGG_T
Cuts at: 365 365
Size: 4343
SfcI C'TryA_G
Cuts at: 33 486 808 883 930 1030 1386 2071 3064
Size: 453 322 75 47 100 356 685 993
Cuts at: 3064 3255 33
Size: 191 1121
Fragments arranged by size:
1121 993 685 453 356 322 191 100
75 47
SfiI GGCCn_nnn'nGGCC
Cuts at: 206 206
Size: 4343
SfoI GGC'GCC
Cuts at: 1775 2023 1775
Size: 248 4095
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4343
SmaI CCC'GGG
Cuts at: 1365 1365
Size: 4343
SmlI C'TyrA_G
Cuts at: 337 1498 2413 2905 3167 337
Size: 1161 915 492 262 1513
Fragments arranged by size:
1513 1161 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4343
SphI G_CATG'C
Cuts at: 1396 1896 1396
Size: 500 3843
StuI AGG'CCT
Cuts at: 233 388 233
Size: 155 4188
StyI C'CwwG_G
Cuts at: 107 971 1307 1477 1831 107
Size: 864 336 170 354 2619
Fragments arranged by size:
2619 864 354 336 170
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 558 1057 1926 2059 2518 2727 558
Size: 499 869 133 459 209 2174
Fragments arranged by size:
2174 869 499 459 209 133
TatI w'GTAC_w
Cuts at: 1301 3858 1301
Size: 2557 1786
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 826 858 3860 3892 826
Size: 32 3002 32 1277
Fragments arranged by size:
3002 1277 32 32
Tth111I GACn'n_nGTC
Cuts at: 2140 2140
Size: 4343
XbaI T'CTAG_A
Cuts at: 1374 1374
Size: 4343
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1838 1838
Size: 4343
XmaI C'CCGG_G
Cuts at: 1363 1363
Size: 4343
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaLI ApoI AscI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI
PfoI PmeI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI ApaI AvrII
BaeI BbvCI BcgI BfrBI BmtI Bpu10I BsiWI BsmI
BsmBI BsrGI BstEII BstXI BstZ17I Bsu36I ClaI CspCI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PmlI PshAI PsiI
PspOMI PspXI PsrI SacII SgrAI SnaBI SrfI SspI
SwaI XhoI XmnI ZraI