Restriction map for
pF1KE0199
(Circular) (Six-base) MAPSORT of: pF1KE0199.seq Check: 515 from: 1 to: 6431
pF1KE0199 6431 bp
With 182 enzymes: SgfI *
October 14, 2008 16:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1079 1079
Size: 6431
AccI GT'mk_AC
Cuts at: 2159 3469 2159
Size: 1310 5121
Acc65I G'GTAC_C
Cuts at: 1684 3447 1684
Size: 1763 4668
AclI AA'CG_TT
Cuts at: 6271 6271
Size: 6431
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 664 1081 1116 1553 1874 2333 2714 4256 4688
Size: 417 35 437 321 459 381 1542 432
Cuts at: 4688 5435 5703 664
Size: 747 268 1392
Fragments arranged by size:
1542 1392 747 459 437 432 417 381
321 268 35
AflIII A'CryG_T
Cuts at: 1100 1251 3783 4887 5864 1100
Size: 151 2532 1104 977 1667
Fragments arranged by size:
2532 1667 1104 977 151
AgeI A'CCGG_T
Cuts at: 1681 4819 1681
Size: 3138 3293
AhdI GACnn_n'nnGTC
Cuts at: 1652 1652
Size: 6431
AleI CACnn'nnGTG
Cuts at: 1011 1085 2618 1011
Size: 74 1533 4824
Fragments arranged by size:
4824 1533 74
AlwNI CAG_nnn'CTG
Cuts at: 2326 3282 3736 5303 2326
Size: 956 454 1567 3454
Fragments arranged by size:
3454 1567 956 454
ApaI G_GGCC'C
Cuts at: 189 304 542 1440 1711 2630 3347 189
Size: 115 238 898 271 919 717 3273
Fragments arranged by size:
3273 919 898 717 271 238 115
ApaLI G'TGCA_C
Cuts at: 865 1014 5201 865
Size: 149 4187 2095
Fragments arranged by size:
4187 2095 149
ApoI r'AATT_y
Cuts at: 841 3024 3406 3435 5784 6040 841
Size: 2183 382 29 2349 256 1232
Fragments arranged by size:
2349 2183 1232 382 256 29
AseI AT'TA_AT
Cuts at: 20 20
Size: 6431
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6431
AvaI C'yCGr_G
Cuts at: 587 617 769 1319 1649 3451 587
Size: 30 152 550 330 1802 3567
Fragments arranged by size:
3567 1802 550 330 152 30
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1668 1676 1701 1709 1668
Size: 8 25 8 6390
Fragments arranged by size:
6390 25 8 8
BamHI G'GATC_C
Cuts at: 732 3456 732
Size: 2724 3707
BanI G'GyrC_C
Cuts at: 487 1028 1295 1323 1408 1684 2564 2595 3447
Size: 541 267 28 85 276 880 31 852
Cuts at: 3447 3861 4109 4144 487
Size: 414 248 35 2774
Fragments arranged by size:
2774 880 852 541 414 276 267 248
85 35 31 28
BanII G_rGCy'C
Cuts at: 155 189 304 499 542 1349 1440 1711 2630
Size: 34 115 195 43 807 91 271 919
Cuts at: 2630 3275 3347 3445 4475 155
Size: 645 72 98 1030 2111
Fragments arranged by size:
2111 1030 919 807 645 271 195 115
98 91 72 43 34
BbeI G_GCGC'C
Cuts at: 3865 4113 3865
Size: 248 6183
BbsI GAAGACnn'nnnn_
Cuts at: 1069 1541 1942 1069
Size: 472 401 5558
Fragments arranged by size:
5558 472 401
BbvCI CC'TCA_GC
Cuts at: 943 943
Size: 6431
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1147 1181 1147
Size: 34 6397
BciVI GTATCCnnnnn_n'
Cuts at: 1984 1994 4329 5090 6431 1984
Size: 10 2335 761 1341 1984
Fragments arranged by size:
2335 1984 1341 761 10
BclI T'GATC_A
Cuts at: 3303 3951 3303
Size: 648 5783
BglI GCCn_nnn'nGGC
Cuts at: 1706 2367 3279 1706
Size: 661 912 4858
Fragments arranged by size:
4858 912 661
BglII A'GATC_T
Cuts at: 3946 5983 3946
Size: 2037 4394
BlpI GC'TnA_GC
Cuts at: 2327 2792 3543 2327
Size: 465 751 5215
Fragments arranged by size:
5215 751 465
Bme1580I G_kGCm'C
Cuts at: 189 304 354 542 869 1018 1326 1440 1711
Size: 115 50 188 327 149 308 114 271
Cuts at: 1711 2569 2630 3347 4056 4149 5205 189
Size: 858 61 717 709 93 1056 1415
Fragments arranged by size:
1415 1056 858 717 709 327 308 271
188 149 115 114 93 61 50
BmrI ACTGGGnnnn_n'
Cuts at: 170 185 930 954 1434 1708 2563 2612 3643
Size: 15 745 24 480 274 855 49 1031
Cuts at: 3643 3724 4059 6203 170
Size: 81 335 2144 398
Fragments arranged by size:
2144 1031 855 745 480 398 335 274
81 49 24 15
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 518 3308 4798 518
Size: 2790 1490 2151
Fragments arranged by size:
2790 2151 1490
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 440 918 1527 1728 2042 2671 2921 440
Size: 478 609 201 314 629 250 3950
Fragments arranged by size:
3950 629 609 478 314 250 201
Bpu10I CC'TnA_GC
Cuts at: 943 943
Size: 6431
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1621 2770 3607 4486 4978 5276 1621
Size: 1149 837 879 492 298 2776
Fragments arranged by size:
2776 1149 879 837 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 274 1660 6135 274
Size: 1386 4475 570
Fragments arranged by size:
4475 1386 570
BsaAI yAC'GTr
Cuts at: 3784 3784
Size: 6431
BsaBI GATnn'nnATC
Cuts at: 6 450 3945 5982 6
Size: 444 3495 2037 455
Fragments arranged by size:
3495 2037 455 444
BsaHI Gr'CG_yC
Cuts at: 1277 3862 4110 4812 6308 1277
Size: 2585 248 702 1496 1400
Fragments arranged by size:
2585 1496 1400 702 248
BsaWI w'CCGG_w
Cuts at: 1681 3620 4141 4819 5093 5240 5651 1681
Size: 1939 521 678 274 147 411 2461
Fragments arranged by size:
2461 1939 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 898 928 1616 1646 2010 2040 2818 2848 3318
Size: 30 688 30 364 30 778 30 470
Cuts at: 3318 3348 898
Size: 30 3981
Fragments arranged by size:
3981 778 688 470 364 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 793 1773 2663 3179 3281 793
Size: 980 890 516 102 3943
Fragments arranged by size:
3943 980 890 516 102
BseYI C'CCAG_C
Cuts at: 415 513 1666 1859 2031 2176 2408 2663 2705
Size: 98 1153 193 172 145 232 255 42
Cuts at: 2705 3133 3856 4614 5191 5687 415
Size: 428 723 758 577 496 1159
Fragments arranged by size:
1159 1153 758 723 577 496 428 255
232 193 172 145 98 42
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 276 347 1197 1499 276
Size: 71 850 302 5208
Fragments arranged by size:
5208 850 302 71
BsiEI CG_ry'CG
Cuts at: 104 3973 4019 5227 104
Size: 3869 46 1208 1308
Fragments arranged by size:
3869 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 869 887 1018 1112 3445 4223 4413 5205 869
Size: 18 131 94 2333 778 190 792 2095
Fragments arranged by size:
2333 2095 792 778 190 131 94 18
BsmI GAATG_Cn'
Cuts at: 2196 2196
Size: 6431
Bsp1286I G_dGCh'C
Cuts at: 155 189 304 354 499 542 869 887 1018
Size: 34 115 50 145 43 327 18 131
Cuts at: 1018 1112 1326 1349 1440 1711 2569 2630 3275
Size: 94 214 23 91 271 858 61 645
Cuts at: 3275 3347 3445 4056 4149 4223 4413 4475 5205
Size: 72 98 611 93 74 190 62 730
Cuts at: 5205 155
Size: 1381
Fragments arranged by size:
1381 858 730 645 611 327 271 214
190 145 131 115 98 94 93 91
74 72 62 61 50 43 34 23
18
BspEI T'CCGG_A
Cuts at: 5651 5651
Size: 6431
BspHI T'CATG_A
Cuts at: 5607 5607
Size: 6431
BspMI ACCTGCnnnn'nnnn_
Cuts at: 398 1079 1877 3481 3695 3997 4378 398
Size: 681 798 1604 214 302 381 2451
Fragments arranged by size:
2451 1604 798 681 381 302 214
BsrBI CCG'CTC
Cuts at: 50 900 1359 1781 4723 4777 6261 6426 50
Size: 850 459 422 2942 54 1484 165 55
Fragments arranged by size:
2942 1484 850 459 422 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1143 1521 1565 3088 4343 1143
Size: 378 44 1523 1255 3231
Fragments arranged by size:
3231 1523 1255 378 44
BsrFI r'CCGG_y
Cuts at: 1681 4429 4610 4819 1681
Size: 2748 181 209 3293
Fragments arranged by size:
3293 2748 209 181
BssSI C'ACGA_G
Cuts at: 1111 4702 5060 1111
Size: 3591 358 2482
Fragments arranged by size:
3591 2482 358
BstAPI GCAn_nnn'nTGC
Cuts at: 864 1477 1869 1885 3808 864
Size: 613 392 16 1923 3487
Fragments arranged by size:
3487 1923 613 392 16
BstBI TT'CG_AA
Cuts at: 4792 4792
Size: 6431
BstEII G'GTnAC_C
Cuts at: 2010 2010
Size: 6431
BstXI CCAn_nnnn'nTGG
Cuts at: 265 568 2592 2898 265
Size: 303 2024 306 3798
Fragments arranged by size:
3798 2024 306 303
BstYI r'GATC_y
Cuts at: 1 732 1230 3385 3456 3923 3946 4281 4527
Size: 731 498 2155 71 467 23 335 246
Cuts at: 4527 5539 5625 5637 5983 1
Size: 1012 86 12 346 449
Fragments arranged by size:
2155 1012 731 498 467 449 346 335
246 86 71 23 12
Bsu36I CC'TnA_GG
Cuts at: 1609 3149 1609
Size: 1540 4891
BtgI C'CryG_G
Cuts at: 891 1299 2198 2243 891
Size: 408 899 45 5079
Fragments arranged by size:
5079 899 408 45
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 459 4381 4562 4725 459
Size: 3922 181 163 2165
Fragments arranged by size:
3922 2165 181 163
BtsI GCAGTG_nn'
Cuts at: 1461 1916 2259 3674 1461
Size: 455 343 1415 4218
Fragments arranged by size:
4218 1415 455 343
DraI TTT'AAA
Cuts at: 3430 3430
Size: 6431
DrdI GACnn_nn'nnGTC
Cuts at: 4137 4995 4137
Size: 858 5573
EaeI y'GGCC_r
Cuts at: 1636 4016 4190 4581 4608 5953 1636
Size: 2380 174 391 27 1345 2114
Fragments arranged by size:
2380 2114 1345 391 174 27
EagI C'GGCC_G
Cuts at: 4016 4016
Size: 6431
EarI CTCTTCn'nnn_
Cuts at: 1595 2216 4454 4664 1595
Size: 621 2238 210 3362
Fragments arranged by size:
3362 2238 621 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4639 4949 5095 6241 4639
Size: 310 146 1146 4829
Fragments arranged by size:
4829 1146 310 146
EcoICRI GAG'CTC
Cuts at: 3443 3443
Size: 6431
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 440 664 918 1081 1116 1527 1553 1728 1874
Size: 224 254 163 35 411 26 175 146
Cuts at: 1874 2042 2333 2671 2714 2921 4256 4688 5435
Size: 168 291 338 43 207 1335 432 747
Cuts at: 5435 5703 440
Size: 268 1168
Fragments arranged by size:
1335 1168 747 432 411 338 291 268
254 224 207 175 168 163 146 43
35 26
EcoNI CCTnn'n_nnAGG
Cuts at: 3194 3194
Size: 6431
EcoO109I rG'GnC_Cy
Cuts at: 185 186 300 538 539 622 765 947 1369
Size: 1 114 238 1 83 143 182 422
Cuts at: 1369 1436 1558 1646 1708 2237 2308 2482 2626
Size: 67 122 88 62 529 71 174 144
Cuts at: 2626 2627 2695 3172 3344 3570 185
Size: 1 68 477 172 226 3046
Fragments arranged by size:
3046 529 477 422 238 226 182 174
172 144 143 122 114 88 83 71
68 67 62 1 1 1
EcoRI G'AATT_C
Cuts at: 3406 3435 3406
Size: 29 6402
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1904 4450 1904
Size: 2546 3885
FspI TGC'GCA
Cuts at: 985 4212 985
Size: 3227 3204
HaeII r_GCGC'y
Cuts at: 3865 4113 5135 6114 3865
Size: 248 1022 979 4182
Fragments arranged by size:
4182 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 591 623 781 813 1222 1254 1358 1390 1638
Size: 32 158 32 409 32 104 32 248
Cuts at: 1638 1670 1932 1964 4273 4303 4305 4335 591
Size: 32 262 32 2309 30 2 30 2687
Fragments arranged by size:
2687 2309 409 262 248 158 104 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 338 3470 338
Size: 3132 3299
KasI G'GCGC_C
Cuts at: 3861 4109 3861
Size: 248 6183
KpnI G_GTAC'C
Cuts at: 1688 3451 1688
Size: 1763 4668
MluI A'CGCG_T
Cuts at: 5864 5864
Size: 6431
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 353 5102 5286 353
Size: 4749 184 1498
Fragments arranged by size:
4749 1498 184
MscI TGG'CCA
Cuts at: 4192 4192
Size: 6431
MslI CAynn'nnrTG
Cuts at: 263 970 1011 1085 1101 1178 2618 3358 4420
Size: 707 41 74 16 77 1440 740 1062
Cuts at: 4420 4547 263
Size: 127 2147
Fragments arranged by size:
2147 1440 1062 740 707 127 77 74
41 16
MspA1I CmG'CkG
Cuts at: 290 642 861 2326 3137 3246 3309 3542 3856
Size: 352 219 1465 811 109 63 233 314
Cuts at: 3856 4216 5229 5474 290
Size: 360 1013 245 1247
Fragments arranged by size:
1465 1247 1013 811 360 352 314 245
233 219 109 63
NaeI GCC'GGC
Cuts at: 4612 4612
Size: 6431
NarI GG'CG_CC
Cuts at: 3862 4110 3862
Size: 248 6183
NcoI C'CATG_G
Cuts at: 2198 2243 2198
Size: 45 6386
NdeI CA'TA_TG
Cuts at: 2949 2949
Size: 6431
NgoMIV G'CCGG_C
Cuts at: 4610 4610
Size: 6431
NruI TCG'CGA
Cuts at: 444 444
Size: 6431
NspI r_CATG'y
Cuts at: 663 969 984 1104 1255 3484 3984 4891 663
Size: 306 15 120 151 2229 500 907 2203
Fragments arranged by size:
2229 2203 907 500 306 151 120 15
PasI CC'CwG_GG
Cuts at: 1432 3276 1432
Size: 1844 4587
PciI A'CATG_T
Cuts at: 1100 1251 4887 1100
Size: 151 3636 2644
Fragments arranged by size:
3636 2644 151
PflMI CCAn_nnn'nTGG
Cuts at: 1432 1825 2233 1432
Size: 393 408 5630
Fragments arranged by size:
5630 408 393
PfoI T'CCnGG_A
Cuts at: 456 1986 456
Size: 1530 4901
PmeI GTTT'AAAC
Cuts at: 3430 3430
Size: 6431
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5570 5602 5570
Size: 32 6399
PpuMI rG'GwC_Cy
Cuts at: 622 765 1369 1558 1646 2237 2308 3172 622
Size: 143 604 189 88 591 71 864 3881
Fragments arranged by size:
3881 864 604 591 189 143 88 71
PspOMI G'GGCC_C
Cuts at: 185 300 538 1436 1707 2626 3343 185
Size: 115 238 898 271 919 717 3273
Fragments arranged by size:
3273 919 898 717 271 238 115
PstI C_TGCA'G
Cuts at: 395 835 1123 1453 1923 3478 4163 395
Size: 440 288 330 470 1555 685 2663
Fragments arranged by size:
2663 1555 685 470 440 330 288
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6431
PvuII CAG'CTG
Cuts at: 290 642 861 2326 3137 3246 3856 4216 290
Size: 352 219 1465 811 109 610 360 2505
Fragments arranged by size:
2505 1465 811 610 360 352 219 109
RsrII CG'GwC_CG
Cuts at: 4626 4626
Size: 6431
SacI G_AGCT'C
Cuts at: 3445 3445
Size: 6431
SalI G'TCGA_C
Cuts at: 3468 3468
Size: 6431
SanDI GG'GwC_CC
Cuts at: 622 1646 622
Size: 1024 5407
SapI GCTCTTCn'nnn_
Cuts at: 2216 4454 4664 2216
Size: 2238 210 3983
Fragments arranged by size:
3983 2238 210
SbfI CC_TGCA'GG
Cuts at: 3478 3478
Size: 6431
ScaI AGT'ACT
Cuts at: 5948 5948
Size: 6431
SfcI C'TryA_G
Cuts at: 33 391 831 1119 1337 1449 1919 2215 2505
Size: 358 440 288 218 112 470 296 290
Cuts at: 2505 3474 4159 5152 5343 33
Size: 969 685 993 191 1121
Fragments arranged by size:
1121 993 969 685 470 440 358 296
290 288 218 191 112
SfoI GGC'GCC
Cuts at: 3863 4111 3863
Size: 248 6183
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6431
SmaI CCC'GGG
Cuts at: 589 619 1651 3453 589
Size: 30 1032 1802 3567
Fragments arranged by size:
3567 1802 1032 30
SmlI C'TyrA_G
Cuts at: 769 1600 2785 3586 4501 4993 5255 769
Size: 831 1185 801 915 492 262 1945
Fragments arranged by size:
1945 1185 915 831 801 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6431
SphI G_CATG'C
Cuts at: 3484 3984 3484
Size: 500 5931
StuI AGG'CCT
Cuts at: 2416 2742 2416
Size: 326 6105
StyI C'CwwG_G
Cuts at: 689 1963 2198 2243 2486 3565 3919 689
Size: 1274 235 45 243 1079 354 3201
Fragments arranged by size:
3201 1274 1079 354 243 235 45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 429 1163 2121 2394 2449 2782 4014 4147 4606
Size: 734 958 273 55 333 1232 133 459
Cuts at: 4606 4815 429
Size: 209 2045
Fragments arranged by size:
2045 1232 958 734 459 333 273 209
133 55
TatI w'GTAC_w
Cuts at: 962 5946 962
Size: 4984 1447
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 898 930 2010 2042 2818 2850 5948 5980 898
Size: 32 1080 32 776 32 3098 32 1349
Fragments arranged by size:
3098 1349 1080 776 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4228 4228
Size: 6431
XbaI T'CTAG_A
Cuts at: 3462 3462
Size: 6431
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1338 2745 3357 3926 1338
Size: 1407 612 569 3843
Fragments arranged by size:
3843 1407 612 569
XhoI C'TCGA_G
Cuts at: 769 769
Size: 6431
XmaI C'CCGG_G
Cuts at: 587 617 1649 3451 587
Size: 30 1032 1802 3567
Fragments arranged by size:
3567 1802 1032 30
XmnI GAAnn'nnTTC
Cuts at: 907 907
Size: 6431
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NruI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AlfI AloI AscI AvrII BfrBI
BmgBI BmtI BsiWI BsmBI BsrGI BssHII BstZ17I ClaI
CspCI DraIII EcoRV FseI FspAI HindIII HpaI MfeI
NheI NotI NsiI PacI PmlI PshAI PsiI PspXI
PsrI SacII SexAI SfiI SgrAI SnaBI SrfI SspI
SwaI ZraI