Restriction map for
pF1KE0200
(Circular) (Six-base) MAPSORT of: pF1KE0200.seq Check: 6419 from: 1 to: 4475
pF1KE0200 4475 bp
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AccI GT'mk_AC
Cuts at: 1513 1513
Size: 4475
Acc65I G'GTAC_C
Cuts at: 1491 1491
Size: 4475
AclI AA'CG_TT
Cuts at: 164 4315 164
Size: 4151 324
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1418 2300 2732 3479 3747 1418
Size: 882 432 747 268 2146
Fragments arranged by size:
2146 882 747 432 268
AflIII A'CryG_T
Cuts at: 1827 2931 3908 1827
Size: 1104 977 2394
Fragments arranged by size:
2394 1104 977
AgeI A'CCGG_T
Cuts at: 2863 2863
Size: 4475
AlwNI CAG_nnn'CTG
Cuts at: 662 693 988 1780 3347 662
Size: 31 295 792 1567 1790
Fragments arranged by size:
1790 1567 792 295 31
ApaLI G'TGCA_C
Cuts at: 3245 3245
Size: 4475
ApoI r'AATT_y
Cuts at: 307 1479 3828 4084 307
Size: 1172 2349 256 698
Fragments arranged by size:
2349 1172 698 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4475
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4475
AvaI C'yCGr_G
Cuts at: 856 1495 856
Size: 639 3836
BamHI G'GATC_C
Cuts at: 1500 1500
Size: 4475
BanI G'GyrC_C
Cuts at: 282 1049 1491 1905 2153 2188 282
Size: 767 442 414 248 35 2569
Fragments arranged by size:
2569 767 442 414 248 35
BanII G_rGCy'C
Cuts at: 359 863 1489 2519 359
Size: 504 626 1030 2315
Fragments arranged by size:
2315 1030 626 504
BbeI G_GCGC'C
Cuts at: 286 1909 2157 286
Size: 1623 248 2604
Fragments arranged by size:
2604 1623 248
BbsI GAAGACnn'nnnn_
Cuts at: 726 726
Size: 4475
BciVI GTATCCnnnnn_n'
Cuts at: 2373 3134 4475 2373
Size: 761 1341 2373
Fragments arranged by size:
2373 1341 761
BclI T'GATC_A
Cuts at: 1995 1995
Size: 4475
BglII A'GATC_T
Cuts at: 1990 4027 1990
Size: 2037 2438
BlpI GC'TnA_GC
Cuts at: 1587 1587
Size: 4475
Bme1580I G_kGCm'C
Cuts at: 2100 2193 3249 2100
Size: 93 1056 3326
Fragments arranged by size:
3326 1056 93
BmgBI CAC'GTC
Cuts at: 1056 1056
Size: 4475
BmrI ACTGGGnnnn_n'
Cuts at: 1687 1768 2103 4247 1687
Size: 81 335 2144 1915
Fragments arranged by size:
2144 1915 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 983 2842 983
Size: 1859 2616
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 469 657 774 1025 469
Size: 188 117 251 3919
Fragments arranged by size:
3919 251 188 117
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1027 1651 2530 3022 3320 1027
Size: 624 879 492 298 2182
Fragments arranged by size:
2182 879 624 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 511 4179 511
Size: 3668 807
BsaAI yAC'GTr
Cuts at: 1828 1828
Size: 4475
BsaBI GATnn'nnATC
Cuts at: 6 1989 4026 6
Size: 1983 2037 455
Fragments arranged by size:
2037 1983 455
BsaHI Gr'CG_yC
Cuts at: 283 1906 2154 2856 4352 283
Size: 1623 248 702 1496 406
Fragments arranged by size:
1623 1496 702 406 248
BsaWI w'CCGG_w
Cuts at: 1664 2185 2863 3137 3284 3695 1664
Size: 521 678 274 147 411 2444
Fragments arranged by size:
2444 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 467 497 467
Size: 30 4445
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 247 250 488 816 892 925 1013 1219 1263
Size: 3 238 328 76 33 88 206 44
Cuts at: 1263 1393 247
Size: 130 3329
Fragments arranged by size:
3329 328 238 206 130 88 76 44
33 3
BseYI C'CCAG_C
Cuts at: 286 468 1900 2658 3235 3731 286
Size: 182 1432 758 577 496 1030
Fragments arranged by size:
1432 1030 758 577 496 182
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 229 229
Size: 4475
BsiEI CG_ry'CG
Cuts at: 104 440 572 2017 2063 3271 104
Size: 336 132 1445 46 1208 1308
Fragments arranged by size:
1445 1308 1208 336 132 46
BsiHKAI G_wGCw'C
Cuts at: 872 1489 2267 2457 3249 872
Size: 617 778 190 792 2098
Fragments arranged by size:
2098 792 778 617 190
BsmBI CGTCTCn'nnnn_
Cuts at: 591 1062 591
Size: 471 4004
Bsp1286I G_dGCh'C
Cuts at: 359 863 872 1489 2100 2193 2267 2457 2519
Size: 504 9 617 611 93 74 190 62
Cuts at: 2519 3249 359
Size: 730 1585
Fragments arranged by size:
1585 730 617 611 504 190 93 74
62 9
BspEI T'CCGG_A
Cuts at: 3695 3695
Size: 4475
BspHI T'CATG_A
Cuts at: 3651 3651
Size: 4475
BspMI ACCTGCnnnn'nnnn_
Cuts at: 692 1525 1739 2041 2422 692
Size: 833 214 302 381 2745
Fragments arranged by size:
2745 833 381 302 214
BsrBI CCG'CTC
Cuts at: 50 350 560 2767 2821 4305 4470 50
Size: 300 210 2207 54 1484 165 55
Fragments arranged by size:
2207 1484 300 210 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2387 2387
Size: 4475
BsrFI r'CCGG_y
Cuts at: 2473 2654 2863 2473
Size: 181 209 4085
Fragments arranged by size:
4085 209 181
BssHII G'CGCG_C
Cuts at: 616 616
Size: 4475
BssSI C'ACGA_G
Cuts at: 2746 3104 2746
Size: 358 4117
BstAPI GCAn_nnn'nTGC
Cuts at: 1852 1852
Size: 4475
BstBI TT'CG_AA
Cuts at: 2836 2836
Size: 4475
BstEII G'GTnAC_C
Cuts at: 456 456
Size: 4475
BstYI r'GATC_y
Cuts at: 1 690 1292 1500 1967 1990 2325 2571 3583
Size: 689 602 208 467 23 335 246 1012
Cuts at: 3583 3669 3681 4027 1
Size: 86 12 346 449
Fragments arranged by size:
1012 689 602 467 449 346 335 246
208 86 23 12
BtgI C'CryG_G
Cuts at: 107 434 648 107
Size: 327 214 3934
Fragments arranged by size:
3934 327 214
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2425 2606 2769 2425
Size: 181 163 4131
Fragments arranged by size:
4131 181 163
BtsI GCAGTG_nn'
Cuts at: 1718 1718
Size: 4475
ClaI AT'CG_AT
Cuts at: 938 938
Size: 4475
DraI TTT'AAA
Cuts at: 1474 1474
Size: 4475
DrdI GACnn_nn'nnGTC
Cuts at: 2181 3039 2181
Size: 858 3617
EaeI y'GGCC_r
Cuts at: 437 569 651 2060 2234 2625 2652 3997 437
Size: 132 82 1409 174 391 27 1345 915
Fragments arranged by size:
1409 1345 915 391 174 132 82 27
EagI C'GGCC_G
Cuts at: 437 569 2060 437
Size: 132 1491 2852
Fragments arranged by size:
2852 1491 132
EarI CTCTTCn'nnn_
Cuts at: 1394 2498 2708 1394
Size: 1104 210 3161
Fragments arranged by size:
3161 1104 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2683 2993 3139 4285 2683
Size: 310 146 1146 2873
Fragments arranged by size:
2873 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1487 1487
Size: 4475
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 469 657 774 1025 1418 2300 2732 3479 3747
Size: 188 117 251 393 882 432 747 268
Cuts at: 3747 469
Size: 1197
Fragments arranged by size:
1197 882 747 432 393 268 251 188
117
EcoO109I rG'GnC_Cy
Cuts at: 1190 1320 1614 1190
Size: 130 294 4051
Fragments arranged by size:
4051 294 130
EcoRI G'AATT_C
Cuts at: 1479 1479
Size: 4475
EcoRV GAT'ATC
Cuts at: 297 297
Size: 4475
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2494 2494
Size: 4475
FspI TGC'GCA
Cuts at: 2256 2256
Size: 4475
HaeII r_GCGC'y
Cuts at: 286 701 797 1087 1245 1909 2157 3179 4158
Size: 415 96 290 158 664 248 1022 979
Cuts at: 4158 286
Size: 603
Fragments arranged by size:
1022 979 664 603 415 290 248 158
96
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 187 219 745 777 976 1008 2317 2347 2349
Size: 32 526 32 199 32 1309 30 2
Cuts at: 2349 2379 187
Size: 30 2283
Fragments arranged by size:
2283 1309 526 199 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1514 1514
Size: 4475
KasI G'GCGC_C
Cuts at: 282 1905 2153 282
Size: 1623 248 2604
Fragments arranged by size:
2604 1623 248
KpnI G_GTAC'C
Cuts at: 1495 1495
Size: 4475
MluI A'CGCG_T
Cuts at: 3908 3908
Size: 4475
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1112 1283 3146 3330 1112
Size: 171 1863 184 2257
Fragments arranged by size:
2257 1863 184 171
MscI TGG'CCA
Cuts at: 2236 2236
Size: 4475
MslI CAynn'nnrTG
Cuts at: 2464 2591 2464
Size: 127 4348
MspA1I CmG'CkG
Cuts at: 447 629 650 1586 1900 2260 3273 3518 447
Size: 182 21 936 314 360 1013 245 1404
Fragments arranged by size:
1404 1013 936 360 314 245 182 21
NaeI GCC'GGC
Cuts at: 2656 2656
Size: 4475
NarI GG'CG_CC
Cuts at: 283 1906 2154 283
Size: 1623 248 2604
Fragments arranged by size:
2604 1623 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4475
NgoMIV G'CCGG_C
Cuts at: 2654 2654
Size: 4475
NspI r_CATG'y
Cuts at: 1528 2028 2935 1528
Size: 500 907 3068
Fragments arranged by size:
3068 907 500
PciI A'CATG_T
Cuts at: 2931 2931
Size: 4475
PfoI T'CCnGG_A
Cuts at: 542 1295 542
Size: 753 3722
PmeI GTTT'AAAC
Cuts at: 1474 1474
Size: 4475
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3614 3646 3614
Size: 32 4443
PpuMI rG'GwC_Cy
Cuts at: 1190 1190
Size: 4475
PstI C_TGCA'G
Cuts at: 275 689 1522 2207 275
Size: 414 833 685 2543
Fragments arranged by size:
2543 833 685 414
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4475
PvuII CAG'CTG
Cuts at: 447 1900 2260 447
Size: 1453 360 2662
Fragments arranged by size:
2662 1453 360
RsrII CG'GwC_CG
Cuts at: 2670 2670
Size: 4475
SacI G_AGCT'C
Cuts at: 1489 1489
Size: 4475
SacII CC_GC'GG
Cuts at: 651 651
Size: 4475
SalI G'TCGA_C
Cuts at: 1512 1512
Size: 4475
SapI GCTCTTCn'nnn_
Cuts at: 2498 2708 2498
Size: 210 4265
SbfI CC_TGCA'GG
Cuts at: 689 1522 689
Size: 833 3642
ScaI AGT'ACT
Cuts at: 3992 3992
Size: 4475
SfcI C'TryA_G
Cuts at: 33 271 685 1518 2203 3196 3387 33
Size: 238 414 833 685 993 191 1121
Fragments arranged by size:
1121 993 833 685 414 238 191
SfoI GGC'GCC
Cuts at: 284 1907 2155 284
Size: 1623 248 2604
Fragments arranged by size:
2604 1623 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4475
SmaI CCC'GGG
Cuts at: 1497 1497
Size: 4475
SmlI C'TyrA_G
Cuts at: 1042 1630 2545 3037 3299 1042
Size: 588 915 492 262 2218
Fragments arranged by size:
2218 915 588 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4475
SphI G_CATG'C
Cuts at: 1528 2028 1528
Size: 500 3975
StyI C'CwwG_G
Cuts at: 107 1325 1609 1963 107
Size: 1218 284 354 2619
Fragments arranged by size:
2619 1218 354 284
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 475 2058 2191 2650 2859 475
Size: 1583 133 459 209 2091
Fragments arranged by size:
2091 1583 459 209 133
TatI w'GTAC_w
Cuts at: 3990 3990
Size: 4475
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 467 499 538 570 1449 1481 3992 4024 467
Size: 32 39 32 879 32 2511 32 918
Fragments arranged by size:
2511 918 879 39 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 834 1138 2272 834
Size: 304 1134 3037
Fragments arranged by size:
3037 1134 304
XbaI T'CTAG_A
Cuts at: 1506 1506
Size: 4475
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1970 1970
Size: 4475
XmaI C'CCGG_G
Cuts at: 1495 1495
Size: 4475
XmnI GAAnn'nnTTC
Cuts at: 307 307
Size: 4475
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PfoI PmeI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmtI Bpu10I BsiWI BsmI BsrGI BstXI BstZ17I Bsu36I
CspCI DraIII EcoNI FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PmlI PshAI PsiI PspOMI PspXI PsrI SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
ZraI