Restriction map for
pF1KE0201
(Circular) (Six-base) MAPSORT of: pF1KE0201.seq Check: 4730 from: 1 to: 3362
pF1KE0201 3362 bp
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AatII G_ACGT'C
Cuts at: 197 197
Size: 3362
AccI GT'mk_AC
Cuts at: 400 400
Size: 3362
Acc65I G'GTAC_C
Cuts at: 378 378
Size: 3362
AclI AA'CG_TT
Cuts at: 3202 3202
Size: 3362
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 238 1187 1619 2366 2634 238
Size: 949 432 747 268 966
Fragments arranged by size:
966 949 747 432 268
AflIII A'CryG_T
Cuts at: 714 1818 2795 714
Size: 1104 977 1281
Fragments arranged by size:
1281 1104 977
AgeI A'CCGG_T
Cuts at: 1750 1750
Size: 3362
AlwNI CAG_nnn'CTG
Cuts at: 667 2234 667
Size: 1567 1795
ApaLI G'TGCA_C
Cuts at: 2132 2132
Size: 3362
ApoI r'AATT_y
Cuts at: 366 2715 2971 366
Size: 2349 256 757
Fragments arranged by size:
2349 757 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3362
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3362
AvaI C'yCGr_G
Cuts at: 251 382 251
Size: 131 3231
BamHI G'GATC_C
Cuts at: 387 387
Size: 3362
BanI G'GyrC_C
Cuts at: 378 792 1040 1075 378
Size: 414 248 35 2665
Fragments arranged by size:
2665 414 248 35
BanII G_rGCy'C
Cuts at: 376 1406 376
Size: 1030 2332
BbeI G_GCGC'C
Cuts at: 796 1044 796
Size: 248 3114
BciVI GTATCCnnnnn_n'
Cuts at: 1260 2021 3362 1260
Size: 761 1341 1260
Fragments arranged by size:
1341 1260 761
BclI T'GATC_A
Cuts at: 882 882
Size: 3362
BglII A'GATC_T
Cuts at: 877 2914 877
Size: 2037 1325
BlpI GC'TnA_GC
Cuts at: 474 474
Size: 3362
Bme1580I G_kGCm'C
Cuts at: 987 1080 2136 987
Size: 93 1056 2213
Fragments arranged by size:
2213 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 574 655 990 3134 574
Size: 81 335 2144 802
Fragments arranged by size:
2144 802 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1729 1729
Size: 3362
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 183 259 183
Size: 76 3286
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 328 538 1417 1909 2207 328
Size: 210 879 492 298 1483
Fragments arranged by size:
1483 879 492 298 210
BsaI GGTCTCn'nnnn_
Cuts at: 3066 3066
Size: 3362
BsaAI yAC'GTr
Cuts at: 715 715
Size: 3362
BsaBI GATnn'nnATC
Cuts at: 6 876 2913 6
Size: 870 2037 455
Fragments arranged by size:
2037 870 455
BsaHI Gr'CG_yC
Cuts at: 194 793 1041 1743 3239 194
Size: 599 248 702 1496 317
Fragments arranged by size:
1496 702 599 317 248
BsaWI w'CCGG_w
Cuts at: 551 1072 1750 2024 2171 2582 551
Size: 521 678 274 147 411 1331
Fragments arranged by size:
1331 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 168 174 787 1545 2122 2618 168
Size: 6 613 758 577 496 912
Fragments arranged by size:
912 758 613 577 496 6
BsiEI CG_ry'CG
Cuts at: 104 155 904 950 2158 104
Size: 51 749 46 1208 1308
Fragments arranged by size:
1308 1208 749 51 46
BsiHKAI G_wGCw'C
Cuts at: 376 1154 1344 2136 376
Size: 778 190 792 1602
Fragments arranged by size:
1602 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 201 201
Size: 3362
Bsp1286I G_dGCh'C
Cuts at: 376 987 1080 1154 1344 1406 2136 376
Size: 611 93 74 190 62 730 1602
Fragments arranged by size:
1602 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2582 2582
Size: 3362
BspHI T'CATG_A
Cuts at: 2538 2538
Size: 3362
BspMI ACCTGCnnnn'nnnn_
Cuts at: 412 626 928 1309 412
Size: 214 302 381 2465
Fragments arranged by size:
2465 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1654 1708 3192 3357 50
Size: 1604 54 1484 165 55
Fragments arranged by size:
1604 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1274 1274
Size: 3362
BsrFI r'CCGG_y
Cuts at: 1360 1541 1750 1360
Size: 181 209 2972
Fragments arranged by size:
2972 209 181
BssSI C'ACGA_G
Cuts at: 1633 1991 1633
Size: 358 3004
BstAPI GCAn_nnn'nTGC
Cuts at: 739 739
Size: 3362
BstBI TT'CG_AA
Cuts at: 1723 1723
Size: 3362
BstXI CCAn_nnnn'nTGG
Cuts at: 207 207
Size: 3362
BstYI r'GATC_y
Cuts at: 1 387 854 877 1212 1458 2470 2556 2568
Size: 386 467 23 335 246 1012 86 12
Cuts at: 2568 2914 1
Size: 346 449
Fragments arranged by size:
1012 467 449 386 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1312 1493 1656 1312
Size: 181 163 3018
Fragments arranged by size:
3018 181 163
BtsI GCAGTG_nn'
Cuts at: 605 605
Size: 3362
DraI TTT'AAA
Cuts at: 361 361
Size: 3362
DrdI GACnn_nn'nnGTC
Cuts at: 1068 1926 1068
Size: 858 2504
EaeI y'GGCC_r
Cuts at: 947 1121 1512 1539 2884 947
Size: 174 391 27 1345 1425
Fragments arranged by size:
1425 1345 391 174 27
EagI C'GGCC_G
Cuts at: 947 947
Size: 3362
EarI CTCTTCn'nnn_
Cuts at: 121 1385 1595 121
Size: 1264 210 1888
Fragments arranged by size:
1888 1264 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1570 1880 2026 3172 1570
Size: 310 146 1146 1760
Fragments arranged by size:
1760 1146 310 146
EcoICRI GAG'CTC
Cuts at: 374 374
Size: 3362
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 183 238 259 1187 1619 2366 2634 183
Size: 55 21 928 432 747 268 911
Fragments arranged by size:
928 911 747 432 268 55 21
EcoO109I rG'GnC_Cy
Cuts at: 185 501 185
Size: 316 3046
EcoRI G'AATT_C
Cuts at: 366 366
Size: 3362
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1381 1381
Size: 3362
FspI TGC'GCA
Cuts at: 1143 1143
Size: 3362
HaeII r_GCGC'y
Cuts at: 796 1044 2066 3045 796
Size: 248 1022 979 1113
Fragments arranged by size:
1113 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1204 1234 1236 1266 1204
Size: 30 2 30 3300
Fragments arranged by size:
3300 30 30 2
HincII GTy'rAC
Cuts at: 401 401
Size: 3362
KasI G'GCGC_C
Cuts at: 792 1040 792
Size: 248 3114
KpnI G_GTAC'C
Cuts at: 382 382
Size: 3362
MluI A'CGCG_T
Cuts at: 2795 2795
Size: 3362
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2033 2217 2033
Size: 184 3178
MscI TGG'CCA
Cuts at: 1123 1123
Size: 3362
MslI CAynn'nnrTG
Cuts at: 1351 1478 1351
Size: 127 3235
MspA1I CmG'CkG
Cuts at: 473 787 1147 2160 2405 473
Size: 314 360 1013 245 1430
Fragments arranged by size:
1430 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1543 1543
Size: 3362
NarI GG'CG_CC
Cuts at: 793 1041 793
Size: 248 3114
NgoMIV G'CCGG_C
Cuts at: 1541 1541
Size: 3362
NspI r_CATG'y
Cuts at: 415 915 1822 415
Size: 500 907 1955
Fragments arranged by size:
1955 907 500
PasI CC'CwG_GG
Cuts at: 180 180
Size: 3362
PciI A'CATG_T
Cuts at: 1818 1818
Size: 3362
PmeI GTTT'AAAC
Cuts at: 361 361
Size: 3362
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2501 2533 2501
Size: 32 3330
PpuMI rG'GwC_Cy
Cuts at: 185 185
Size: 3362
PstI C_TGCA'G
Cuts at: 409 1094 409
Size: 685 2677
PvuI CG_AT'CG
Cuts at: 104 155 104
Size: 51 3311
PvuII CAG'CTG
Cuts at: 787 1147 787
Size: 360 3002
RsrII CG'GwC_CG
Cuts at: 1557 1557
Size: 3362
SacI G_AGCT'C
Cuts at: 376 376
Size: 3362
SalI G'TCGA_C
Cuts at: 399 399
Size: 3362
SanDI GG'GwC_CC
Cuts at: 185 185
Size: 3362
SapI GCTCTTCn'nnn_
Cuts at: 121 1385 1595 121
Size: 1264 210 1888
Fragments arranged by size:
1888 1264 210
SbfI CC_TGCA'GG
Cuts at: 409 409
Size: 3362
ScaI AGT'ACT
Cuts at: 2879 2879
Size: 3362
SfcI C'TryA_G
Cuts at: 33 405 1090 2083 2274 33
Size: 372 685 993 191 1121
Fragments arranged by size:
1121 993 685 372 191
SfoI GGC'GCC
Cuts at: 794 1042 794
Size: 248 3114
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3362
SmaI CCC'GGG
Cuts at: 384 384
Size: 3362
SmlI C'TyrA_G
Cuts at: 343 517 1432 1924 2186 343
Size: 174 915 492 262 1519
Fragments arranged by size:
1519 915 492 262 174
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3362
SphI G_CATG'C
Cuts at: 415 915 415
Size: 500 2862
StyI C'CwwG_G
Cuts at: 206 496 850 206
Size: 290 354 2718
Fragments arranged by size:
2718 354 290
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 945 1078 1537 1746 945
Size: 133 459 209 2561
Fragments arranged by size:
2561 459 209 133
TatI w'GTAC_w
Cuts at: 2877 2877
Size: 3362
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2879 2911 2879
Size: 32 3330
Tth111I GACn'n_nGTC
Cuts at: 1159 1159
Size: 3362
XbaI T'CTAG_A
Cuts at: 393 393
Size: 3362
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 857 857
Size: 3362
XmaI C'CCGG_G
Cuts at: 382 382
Size: 3362
ZraI GAC'GTC
Cuts at: 195 195
Size: 3362
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PasI PciI PmeI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BsaXI BseRI BsgI BsiWI BsmI
BsrGI BssHII BstEII BstZ17I Bsu36I BtgI ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PflMI
PfoI PmlI PshAI PsiI PspOMI PspXI PsrI SacII
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI