Restriction map for
pF1KE0203
(Circular) (Six-base) MAPSORT of: pF1KE0203.seq Check: 2745 from: 1 to: 4016
pF1KE0203 4016 bp
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AccI GT'mk_AC
Cuts at: 1054 1054
Size: 4016
Acc65I G'GTAC_C
Cuts at: 1032 1032
Size: 4016
AclI AA'CG_TT
Cuts at: 3856 3856
Size: 4016
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 419 1022 1841 2273 3020 3288 419
Size: 603 819 432 747 268 1147
Fragments arranged by size:
1147 819 747 603 432 268
AflII C'TTAA_G
Cuts at: 348 348
Size: 4016
AflIII A'CryG_T
Cuts at: 1368 2472 3449 1368
Size: 1104 977 1935
Fragments arranged by size:
1935 1104 977
AgeI A'CCGG_T
Cuts at: 2404 2404
Size: 4016
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 995 995
Size: 4016
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 245 277 245
Size: 32 3984
AlwNI CAG_nnn'CTG
Cuts at: 1321 2888 1321
Size: 1567 2449
ApaLI G'TGCA_C
Cuts at: 2786 2786
Size: 4016
ApoI r'AATT_y
Cuts at: 373 533 1020 3369 3625 373
Size: 160 487 2349 256 764
Fragments arranged by size:
2349 764 487 256 160
AseI AT'TA_AT
Cuts at: 20 322 20
Size: 302 3714
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4016
AvaI C'yCGr_G
Cuts at: 1036 1036
Size: 4016
AvrII C'CTAG_G
Cuts at: 780 780
Size: 4016
BamHI G'GATC_C
Cuts at: 1041 1041
Size: 4016
BanI G'GyrC_C
Cuts at: 1032 1446 1694 1729 1032
Size: 414 248 35 3319
Fragments arranged by size:
3319 414 248 35
BanII G_rGCy'C
Cuts at: 1030 2060 1030
Size: 1030 2986
BbeI G_GCGC'C
Cuts at: 1450 1698 1450
Size: 248 3768
BbsI GAAGACnn'nnnn_
Cuts at: 407 884 407
Size: 477 3539
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3982
BciVI GTATCCnnnnn_n'
Cuts at: 1914 2675 4016 1914
Size: 761 1341 1914
Fragments arranged by size:
1914 1341 761
BclI T'GATC_A
Cuts at: 1536 1536
Size: 4016
BglII A'GATC_T
Cuts at: 1531 3568 1531
Size: 2037 1979
BlpI GC'TnA_GC
Cuts at: 1128 1128
Size: 4016
Bme1580I G_kGCm'C
Cuts at: 441 816 1641 1734 2790 441
Size: 375 825 93 1056 1667
Fragments arranged by size:
1667 1056 825 375 93
BmrI ACTGGGnnnn_n'
Cuts at: 444 1228 1309 1644 3788 444
Size: 784 81 335 2144 672
Fragments arranged by size:
2144 784 672 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2383 2383
Size: 4016
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 176 185 176
Size: 9 4007
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1192 2071 2563 2861 1192
Size: 879 492 298 2347
Fragments arranged by size:
2347 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3720 3720
Size: 4016
BsaAI yAC'GTr
Cuts at: 1369 1369
Size: 4016
BsaBI GATnn'nnATC
Cuts at: 6 415 845 1530 3567 6
Size: 409 430 685 2037 455
Fragments arranged by size:
2037 685 455 430 409
BsaHI Gr'CG_yC
Cuts at: 1447 1695 2397 3893 1447
Size: 248 702 1496 1570
Fragments arranged by size:
1570 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1205 1726 2404 2678 2825 3236 1205
Size: 521 678 274 147 411 1985
Fragments arranged by size:
1985 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 162 192 245 275 530 560 162
Size: 30 53 30 255 30 3618
Fragments arranged by size:
3618 255 53 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 871 871
Size: 4016
BseYI C'CCAG_C
Cuts at: 1441 2199 2776 3272 1441
Size: 758 577 496 2185
Fragments arranged by size:
2185 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1558 1604 2812 104
Size: 1454 46 1208 1308
Fragments arranged by size:
1454 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 258 434 1030 1808 1998 2790 258
Size: 176 596 778 190 792 1484
Fragments arranged by size:
1484 792 778 596 190 176
Bsp1286I G_dGCh'C
Cuts at: 258 434 441 816 1030 1641 1734 1808 1998
Size: 176 7 375 214 611 93 74 190
Cuts at: 1998 2060 2790 258
Size: 62 730 1484
Fragments arranged by size:
1484 730 611 375 214 190 176 93
74 62 7
BspEI T'CCGG_A
Cuts at: 3236 3236
Size: 4016
BspHI T'CATG_A
Cuts at: 390 3192 390
Size: 2802 1214
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1066 1280 1582 1963 1066
Size: 214 302 381 3119
Fragments arranged by size:
3119 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2308 2362 3846 4011 50
Size: 2258 54 1484 165 55
Fragments arranged by size:
2258 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1928 1928
Size: 4016
BsrFI r'CCGG_y
Cuts at: 2014 2195 2404 2014
Size: 181 209 3626
Fragments arranged by size:
3626 209 181
BssSI C'ACGA_G
Cuts at: 2287 2645 2287
Size: 358 3658
BstAPI GCAn_nnn'nTGC
Cuts at: 1393 1393
Size: 4016
BstBI TT'CG_AA
Cuts at: 2377 2377
Size: 4016
BstYI r'GATC_y
Cuts at: 1 630 1041 1508 1531 1866 2112 3124 3210
Size: 629 411 467 23 335 246 1012 86
Cuts at: 3210 3222 3568 1
Size: 12 346 449
Fragments arranged by size:
1012 629 467 449 411 346 335 246
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1966 2147 2310 1966
Size: 181 163 3672
Fragments arranged by size:
3672 181 163
BtsI GCAGTG_nn'
Cuts at: 937 1259 937
Size: 322 3694
DraI TTT'AAA
Cuts at: 527 923 957 1015 527
Size: 396 34 58 3528
Fragments arranged by size:
3528 396 58 34
DrdI GACnn_nn'nnGTC
Cuts at: 1722 2580 1722
Size: 858 3158
EaeI y'GGCC_r
Cuts at: 1601 1775 2166 2193 3538 1601
Size: 174 391 27 1345 2079
Fragments arranged by size:
2079 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1601 1601
Size: 4016
EarI CTCTTCn'nnn_
Cuts at: 233 692 821 923 2039 2249 233
Size: 459 129 102 1116 210 2000
Fragments arranged by size:
2000 1116 459 210 129 102
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2224 2534 2680 3826 2224
Size: 310 146 1146 2414
Fragments arranged by size:
2414 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1028 1028
Size: 4016
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 176 185 419 1022 1841 2273 3020 3288 176
Size: 9 234 603 819 432 747 268 904
Fragments arranged by size:
904 819 747 603 432 268 234 9
EcoO109I rG'GnC_Cy
Cuts at: 1155 1155
Size: 4016
EcoRI G'AATT_C
Cuts at: 533 1020 533
Size: 487 3529
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2035 2035
Size: 4016
FspI TGC'GCA
Cuts at: 1797 1797
Size: 4016
HaeII r_GCGC'y
Cuts at: 1450 1698 2720 3699 1450
Size: 248 1022 979 1767
Fragments arranged by size:
1767 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 226 258 530 562 756 788 970 1002 1858
Size: 32 272 32 194 32 182 32 856
Cuts at: 1858 1888 1890 1920 226
Size: 30 2 30 2322
Fragments arranged by size:
2322 856 272 194 182 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1055 1055
Size: 4016
KasI G'GCGC_C
Cuts at: 1446 1694 1446
Size: 248 3768
KpnI G_GTAC'C
Cuts at: 1036 1036
Size: 4016
MluI A'CGCG_T
Cuts at: 3449 3449
Size: 4016
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2687 2871 2687
Size: 184 3832
MscI TGG'CCA
Cuts at: 1777 1777
Size: 4016
MslI CAynn'nnrTG
Cuts at: 2005 2132 2005
Size: 127 3889
MspA1I CmG'CkG
Cuts at: 1127 1441 1801 2814 3059 1127
Size: 314 360 1013 245 2084
Fragments arranged by size:
2084 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2197 2197
Size: 4016
NarI GG'CG_CC
Cuts at: 1447 1695 1447
Size: 248 3768
NgoMIV G'CCGG_C
Cuts at: 2195 2195
Size: 4016
NspI r_CATG'y
Cuts at: 160 358 1069 1569 2476 160
Size: 198 711 500 907 1700
Fragments arranged by size:
1700 907 711 500 198
PasI CC'CwG_GG
Cuts at: 809 809
Size: 4016
PciI A'CATG_T
Cuts at: 2472 2472
Size: 4016
PmeI GTTT'AAAC
Cuts at: 1015 1015
Size: 4016
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 245 277 3155 3187 245
Size: 32 2878 32 1074
Fragments arranged by size:
2878 1074 32 32
PstI C_TGCA'G
Cuts at: 464 1063 1748 464
Size: 599 685 2732
Fragments arranged by size:
2732 685 599
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4016
PvuII CAG'CTG
Cuts at: 1441 1801 1441
Size: 360 3656
RsrII CG'GwC_CG
Cuts at: 2211 2211
Size: 4016
SacI G_AGCT'C
Cuts at: 1030 1030
Size: 4016
SalI G'TCGA_C
Cuts at: 1053 1053
Size: 4016
SapI GCTCTTCn'nnn_
Cuts at: 692 2039 2249 692
Size: 1347 210 2459
Fragments arranged by size:
2459 1347 210
SbfI CC_TGCA'GG
Cuts at: 1063 1063
Size: 4016
ScaI AGT'ACT
Cuts at: 3533 3533
Size: 4016
SfcI C'TryA_G
Cuts at: 33 460 1059 1744 2737 2928 33
Size: 427 599 685 993 191 1121
Fragments arranged by size:
1121 993 685 599 427 191
SfoI GGC'GCC
Cuts at: 1448 1696 1448
Size: 248 3768
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4016
SmaI CCC'GGG
Cuts at: 1038 1038
Size: 4016
SmlI C'TyrA_G
Cuts at: 348 1171 2086 2578 2840 348
Size: 823 915 492 262 1524
Fragments arranged by size:
1524 915 823 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4016
SphI G_CATG'C
Cuts at: 1069 1569 1069
Size: 500 3516
SspI AAT'ATT
Cuts at: 996 996
Size: 4016
StyI C'CwwG_G
Cuts at: 737 780 1150 1504 737
Size: 43 370 354 3249
Fragments arranged by size:
3249 370 354 43
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 822 1599 1732 2191 2400 822
Size: 777 133 459 209 2438
Fragments arranged by size:
2438 777 459 209 133
TatI w'GTAC_w
Cuts at: 3531 3531
Size: 4016
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3533 3565 3533
Size: 32 3984
Tth111I GACn'n_nGTC
Cuts at: 1813 1813
Size: 4016
XbaI T'CTAG_A
Cuts at: 1047 1047
Size: 4016
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1511 1511
Size: 4016
XmaI C'CCGG_G
Cuts at: 1036 1036
Size: 4016
XmnI GAAnn'nnTTC
Cuts at: 173 881 173
Size: 708 3308
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlfI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PasI PciI PmeI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI ApaI AscI BaeI
BbvCI BfrBI BglI BmgBI BmtI Bpu10I BsgI BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I
BtgI ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NcoI NdeI NheI NotI NruI
NsiI PacI PflMI PfoI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI StuI SwaI XhoI ZraI