Restriction map for
pF1KE0205
(Circular) (Six-base) MAPSORT of: pF1KE0205.seq Check: 3834 from: 1 to: 3755
pF1KE0205 3755 bp
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AccI GT'mk_AC
Cuts at: 793 793
Size: 3755
Acc65I G'GTAC_C
Cuts at: 771 771
Size: 3755
AclI AA'CG_TT
Cuts at: 3595 3595
Size: 3755
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 314 517 1580 2012 2759 3027 314
Size: 203 1063 432 747 268 1042
Fragments arranged by size:
1063 1042 747 432 268 203
AflIII A'CryG_T
Cuts at: 1107 2211 3188 1107
Size: 1104 977 1674
Fragments arranged by size:
1674 1104 977
AgeI A'CCGG_T
Cuts at: 134 2143 134
Size: 2009 1746
AlwNI CAG_nnn'CTG
Cuts at: 1060 2627 1060
Size: 1567 2188
ApaLI G'TGCA_C
Cuts at: 2525 2525
Size: 3755
ApoI r'AATT_y
Cuts at: 646 759 3108 3364 646
Size: 113 2349 256 1037
Fragments arranged by size:
2349 1037 256 113
AseI AT'TA_AT
Cuts at: 20 20
Size: 3755
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3755
AvaI C'yCGr_G
Cuts at: 488 775 488
Size: 287 3468
BamHI G'GATC_C
Cuts at: 780 780
Size: 3755
BanI G'GyrC_C
Cuts at: 113 457 771 1185 1433 1468 113
Size: 344 314 414 248 35 2400
Fragments arranged by size:
2400 414 344 314 248 35
BanII G_rGCy'C
Cuts at: 769 1799 769
Size: 1030 2725
BbeI G_GCGC'C
Cuts at: 117 1189 1437 117
Size: 1072 248 2435
Fragments arranged by size:
2435 1072 248
BbsI GAAGACnn'nnnn_
Cuts at: 281 659 281
Size: 378 3377
BciVI GTATCCnnnnn_n'
Cuts at: 1653 2414 3755 1653
Size: 761 1341 1653
Fragments arranged by size:
1653 1341 761
BclI T'GATC_A
Cuts at: 1275 1275
Size: 3755
BglI GCCn_nnn'nGGC
Cuts at: 304 304
Size: 3755
BglII A'GATC_T
Cuts at: 1270 3307 1270
Size: 2037 1718
BlpI GC'TnA_GC
Cuts at: 867 867
Size: 3755
Bme1580I G_kGCm'C
Cuts at: 1380 1473 2529 1380
Size: 93 1056 2606
Fragments arranged by size:
2606 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 704 967 1048 1383 3527 704
Size: 263 81 335 2144 932
Fragments arranged by size:
2144 932 335 263 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 394 2122 394
Size: 1728 2027
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 322 424 322
Size: 102 3653
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 931 1810 2302 2600 931
Size: 879 492 298 2086
Fragments arranged by size:
2086 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3459 3459
Size: 3755
BsaAI yAC'GTr
Cuts at: 1108 1108
Size: 3755
BsaBI GATnn'nnATC
Cuts at: 6 202 604 1269 3306 6
Size: 196 402 665 2037 455
Fragments arranged by size:
2037 665 455 402 196
BsaHI Gr'CG_yC
Cuts at: 114 591 1186 1434 2136 3632 114
Size: 477 595 248 702 1496 237
Fragments arranged by size:
1496 702 595 477 248 237
BsaWI w'CCGG_w
Cuts at: 134 944 1465 2143 2417 2564 2975 134
Size: 810 521 678 274 147 411 914
Fragments arranged by size:
914 810 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 556 556
Size: 3755
BseYI C'CCAG_C
Cuts at: 424 1180 1938 2515 3011 424
Size: 756 758 577 496 1168
Fragments arranged by size:
1168 758 756 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 727 727
Size: 3755
BsiEI CG_ry'CG
Cuts at: 104 1297 1343 2551 104
Size: 1193 46 1208 1308
Fragments arranged by size:
1308 1208 1193 46
BsiHKAI G_wGCw'C
Cuts at: 769 1547 1737 2529 769
Size: 778 190 792 1995
Fragments arranged by size:
1995 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 769 1380 1473 1547 1737 1799 2529 769
Size: 611 93 74 190 62 730 1995
Fragments arranged by size:
1995 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2975 2975
Size: 3755
BspHI T'CATG_A
Cuts at: 2931 2931
Size: 3755
BspMI ACCTGCnnnn'nnnn_
Cuts at: 805 1019 1321 1702 805
Size: 214 302 381 2858
Fragments arranged by size:
2858 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2047 2101 3585 3750 50
Size: 1997 54 1484 165 55
Fragments arranged by size:
1997 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1667 1667
Size: 3755
BsrFI r'CCGG_y
Cuts at: 134 1753 1934 2143 134
Size: 1619 181 209 1746
Fragments arranged by size:
1746 1619 209 181
BssSI C'ACGA_G
Cuts at: 2026 2384 2026
Size: 358 3397
BstAPI GCAn_nnn'nTGC
Cuts at: 1132 1132
Size: 3755
BstBI TT'CG_AA
Cuts at: 2116 2116
Size: 3755
BstYI r'GATC_y
Cuts at: 1 197 780 1247 1270 1605 1851 2863 2949
Size: 196 583 467 23 335 246 1012 86
Cuts at: 2949 2961 3307 1
Size: 12 346 449
Fragments arranged by size:
1012 583 467 449 346 335 246 196
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3755
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1705 1886 2049 1705
Size: 181 163 3411
Fragments arranged by size:
3411 181 163
BtsI GCAGTG_nn'
Cuts at: 998 998
Size: 3755
DraI TTT'AAA
Cuts at: 754 754
Size: 3755
DraIII CAC_nnn'GTG
Cuts at: 253 264 253
Size: 11 3744
DrdI GACnn_nn'nnGTC
Cuts at: 1461 2319 1461
Size: 858 2897
EaeI y'GGCC_r
Cuts at: 255 662 1340 1514 1905 1932 3277 255
Size: 407 678 174 391 27 1345 733
Fragments arranged by size:
1345 733 678 407 391 174 27
EagI C'GGCC_G
Cuts at: 1340 1340
Size: 3755
EarI CTCTTCn'nnn_
Cuts at: 1778 1988 1778
Size: 210 3545
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1963 2273 2419 3565 1963
Size: 310 146 1146 2153
Fragments arranged by size:
2153 1146 310 146
EcoICRI GAG'CTC
Cuts at: 767 767
Size: 3755
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 314 322 424 517 1580 2012 2759 3027 314
Size: 8 102 93 1063 432 747 268 1042
Fragments arranged by size:
1063 1042 747 432 268 102 93 8
EcoNI CCTnn'n_nnAGG
Cuts at: 564 564
Size: 3755
EcoO109I rG'GnC_Cy
Cuts at: 894 894
Size: 3755
EcoRI G'AATT_C
Cuts at: 759 759
Size: 3755
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 639 1774 639
Size: 1135 2620
FspI TGC'GCA
Cuts at: 565 1536 565
Size: 971 2784
HaeII r_GCGC'y
Cuts at: 117 1189 1437 2459 3438 117
Size: 1072 248 1022 979 434
Fragments arranged by size:
1072 1022 979 434 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 271 303 1597 1627 1629 1659 271
Size: 32 1294 30 2 30 2367
Fragments arranged by size:
2367 1294 32 30 30 2
HincII GTy'rAC
Cuts at: 794 794
Size: 3755
HindIII A'AGCT_T
Cuts at: 529 529
Size: 3755
KasI G'GCGC_C
Cuts at: 113 1185 1433 113
Size: 1072 248 2435
Fragments arranged by size:
2435 1072 248
KpnI G_GTAC'C
Cuts at: 775 775
Size: 3755
MluI A'CGCG_T
Cuts at: 3188 3188
Size: 3755
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2426 2610 2426
Size: 184 3571
MscI TGG'CCA
Cuts at: 257 664 1516 257
Size: 407 852 2496
Fragments arranged by size:
2496 852 407
MslI CAynn'nnrTG
Cuts at: 370 1744 1871 370
Size: 1374 127 2254
Fragments arranged by size:
2254 1374 127
MspA1I CmG'CkG
Cuts at: 285 866 1180 1540 2553 2798 285
Size: 581 314 360 1013 245 1242
Fragments arranged by size:
1242 1013 581 360 314 245
NaeI GCC'GGC
Cuts at: 1936 1936
Size: 3755
NarI GG'CG_CC
Cuts at: 114 1186 1434 114
Size: 1072 248 2435
Fragments arranged by size:
2435 1072 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 3755
NgoMIV G'CCGG_C
Cuts at: 1934 1934
Size: 3755
NspI r_CATG'y
Cuts at: 369 550 808 1308 2215 369
Size: 181 258 500 907 1909
Fragments arranged by size:
1909 907 500 258 181
PasI CC'CwG_GG
Cuts at: 474 474
Size: 3755
PciI A'CATG_T
Cuts at: 2211 2211
Size: 3755
PflMI CCAn_nnn'nTGG
Cuts at: 253 617 253
Size: 364 3391
PmeI GTTT'AAAC
Cuts at: 754 754
Size: 3755
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2894 2926 2894
Size: 32 3723
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 139 171 139
Size: 32 3723
PstI C_TGCA'G
Cuts at: 802 1487 802
Size: 685 3070
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3755
PvuII CAG'CTG
Cuts at: 285 1180 1540 285
Size: 895 360 2500
Fragments arranged by size:
2500 895 360
RsrII CG'GwC_CG
Cuts at: 1950 1950
Size: 3755
SacI G_AGCT'C
Cuts at: 769 769
Size: 3755
SalI G'TCGA_C
Cuts at: 792 792
Size: 3755
SapI GCTCTTCn'nnn_
Cuts at: 1778 1988 1778
Size: 210 3545
SbfI CC_TGCA'GG
Cuts at: 802 802
Size: 3755
ScaI AGT'ACT
Cuts at: 3272 3272
Size: 3755
SfcI C'TryA_G
Cuts at: 33 482 798 1483 2476 2667 33
Size: 449 316 685 993 191 1121
Fragments arranged by size:
1121 993 685 449 316 191
SfoI GGC'GCC
Cuts at: 115 1187 1435 115
Size: 1072 248 2435
Fragments arranged by size:
2435 1072 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3755
SmaI CCC'GGG
Cuts at: 490 777 490
Size: 287 3468
SmlI C'TyrA_G
Cuts at: 910 1825 2317 2579 910
Size: 915 492 262 2086
Fragments arranged by size:
2086 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3755
SphI G_CATG'C
Cuts at: 808 1308 808
Size: 500 3255
StyI C'CwwG_G
Cuts at: 107 722 889 1243 107
Size: 615 167 354 2619
Fragments arranged by size:
2619 615 354 167
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1338 1471 1930 2139 1338
Size: 133 459 209 2954
Fragments arranged by size:
2954 459 209 133
TatI w'GTAC_w
Cuts at: 3270 3270
Size: 3755
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3272 3304 3272
Size: 32 3723
Tth111I GACn'n_nGTC
Cuts at: 1552 1552
Size: 3755
XbaI T'CTAG_A
Cuts at: 786 786
Size: 3755
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 330 1250 330
Size: 920 2835
XmaI C'CCGG_G
Cuts at: 488 775 488
Size: 287 3468
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PmeI PpiI PsrI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI
BmtI Bpu10I BsaXI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI CspCI EcoRV FseI
FspAI HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI