Restriction map for
pF1KE0206
(Circular) (Six-base) MAPSORT of: pF1KE0206.seq Check: 0 from: 1 to: 3791
pF1KE0206 3791 bp
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AccI GT'mk_AC
Cuts at: 829 829
Size: 3791
Acc65I G'GTAC_C
Cuts at: 807 807
Size: 3791
AclI AA'CG_TT
Cuts at: 3631 3631
Size: 3791
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 577 632 660 1616 2048 2795 3063 577
Size: 55 28 956 432 747 268 1305
Fragments arranged by size:
1305 956 747 432 268 55 28
AflIII A'CryG_T
Cuts at: 1143 2247 3224 1143
Size: 1104 977 1710
Fragments arranged by size:
1710 1104 977
AgeI A'CCGG_T
Cuts at: 2179 2179
Size: 3791
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 719 719
Size: 3791
AlwNI CAG_nnn'CTG
Cuts at: 1096 2663 1096
Size: 1567 2224
ApaLI G'TGCA_C
Cuts at: 2561 2561
Size: 3791
ApoI r'AATT_y
Cuts at: 795 3144 3400 795
Size: 2349 256 1186
Fragments arranged by size:
2349 1186 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3791
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3791
AvaI C'yCGr_G
Cuts at: 811 811
Size: 3791
BamHI G'GATC_C
Cuts at: 816 816
Size: 3791
BanI G'GyrC_C
Cuts at: 186 217 807 1221 1469 1504 186
Size: 31 590 414 248 35 2473
Fragments arranged by size:
2473 590 414 248 35 31
BanII G_rGCy'C
Cuts at: 201 805 1835 201
Size: 604 1030 2157
Fragments arranged by size:
2157 1030 604
BbeI G_GCGC'C
Cuts at: 1225 1473 1225
Size: 248 3543
BbsI GAAGACnn'nnnn_
Cuts at: 228 228
Size: 3791
BbvCI CC'TCA_GC
Cuts at: 369 369
Size: 3791
BciVI GTATCCnnnnn_n'
Cuts at: 1689 2450 3791 1689
Size: 761 1341 1689
Fragments arranged by size:
1689 1341 761
BclI T'GATC_A
Cuts at: 249 1311 249
Size: 1062 2729
BglII A'GATC_T
Cuts at: 1306 3343 1306
Size: 2037 1754
BlpI GC'TnA_GC
Cuts at: 903 903
Size: 3791
Bme1580I G_kGCm'C
Cuts at: 565 1416 1509 2565 565
Size: 851 93 1056 1791
Fragments arranged by size:
1791 1056 851 93
BmrI ACTGGGnnnn_n'
Cuts at: 1003 1084 1419 3563 1003
Size: 81 335 2144 1231
Fragments arranged by size:
2144 1231 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 215 302 2158 215
Size: 87 1856 1848
Fragments arranged by size:
1856 1848 87
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 322 505 322
Size: 183 3608
Bpu10I CC'TnA_GC
Cuts at: 115 369 115
Size: 254 3537
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 125 453 967 1846 2338 2636 125
Size: 328 514 879 492 298 1280
Fragments arranged by size:
1280 879 514 492 328 298
BsaI GGTCTCn'nnnn_
Cuts at: 129 571 3495 129
Size: 442 2924 425
Fragments arranged by size:
2924 442 425
BsaAI yAC'GTr
Cuts at: 1144 1144
Size: 3791
BsaBI GATnn'nnATC
Cuts at: 6 1305 3342 6
Size: 1299 2037 455
Fragments arranged by size:
2037 1299 455
BsaHI Gr'CG_yC
Cuts at: 1222 1470 2172 3668 1222
Size: 248 702 1496 1345
Fragments arranged by size:
1496 1345 702 248
BsaWI w'CCGG_w
Cuts at: 980 1501 2179 2453 2600 3011 980
Size: 521 678 274 147 411 1760
Fragments arranged by size:
1760 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 223 253 223
Size: 30 3761
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 146 589 697 700 146
Size: 443 108 3 3237
Fragments arranged by size:
3237 443 108 3
BseYI C'CCAG_C
Cuts at: 174 453 1216 1974 2551 3047 174
Size: 279 763 758 577 496 918
Fragments arranged by size:
918 763 758 577 496 279
BsiEI CG_ry'CG
Cuts at: 104 1333 1379 2587 104
Size: 1229 46 1208 1308
Fragments arranged by size:
1308 1229 1208 46
BsiHKAI G_wGCw'C
Cuts at: 201 805 1583 1773 2565 201
Size: 604 778 190 792 1427
Fragments arranged by size:
1427 792 778 604 190
Bsp1286I G_dGCh'C
Cuts at: 201 565 805 1416 1509 1583 1773 1835 2565
Size: 364 240 611 93 74 190 62 730
Cuts at: 2565 201
Size: 1427
Fragments arranged by size:
1427 730 611 364 240 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3011 3011
Size: 3791
BspHI T'CATG_A
Cuts at: 2967 2967
Size: 3791
BspMI ACCTGCnnnn'nnnn_
Cuts at: 841 1055 1357 1738 841
Size: 214 302 381 2894
Fragments arranged by size:
2894 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2083 2137 3621 3786 50
Size: 2033 54 1484 165 55
Fragments arranged by size:
2033 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 315 1703 315
Size: 1388 2403
BsrFI r'CCGG_y
Cuts at: 1789 1970 2179 1789
Size: 181 209 3401
Fragments arranged by size:
3401 209 181
BssSI C'ACGA_G
Cuts at: 2062 2420 2062
Size: 358 3433
BstAPI GCAn_nnn'nTGC
Cuts at: 338 1168 338
Size: 830 2961
BstBI TT'CG_AA
Cuts at: 2152 2152
Size: 3791
BstXI CCAn_nnnn'nTGG
Cuts at: 174 174
Size: 3791
BstYI r'GATC_y
Cuts at: 1 260 816 1283 1306 1641 1887 2899 2985
Size: 259 556 467 23 335 246 1012 86
Cuts at: 2985 2997 3343 1
Size: 12 346 449
Fragments arranged by size:
1012 556 467 449 346 335 259 246
86 23 12
BtgI C'CryG_G
Cuts at: 491 491
Size: 3791
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1741 1922 2085 1741
Size: 181 163 3447
Fragments arranged by size:
3447 181 163
BtsI GCAGTG_nn'
Cuts at: 327 1034 327
Size: 707 3084
DraI TTT'AAA
Cuts at: 273 790 273
Size: 517 3274
DrdI GACnn_nn'nnGTC
Cuts at: 1497 2355 1497
Size: 858 2933
EaeI y'GGCC_r
Cuts at: 1376 1550 1941 1968 3313 1376
Size: 174 391 27 1345 1854
Fragments arranged by size:
1854 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1376 1376
Size: 3791
EarI CTCTTCn'nnn_
Cuts at: 189 770 1814 2024 189
Size: 581 1044 210 1956
Fragments arranged by size:
1956 1044 581 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1999 2309 2455 3601 1999
Size: 310 146 1146 2189
Fragments arranged by size:
2189 1146 310 146
EcoICRI GAG'CTC
Cuts at: 199 803 199
Size: 604 3187
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 322 505 577 632 660 1616 2048 2795 3063
Size: 183 72 55 28 956 432 747 268
Cuts at: 3063 322
Size: 1050
Fragments arranged by size:
1050 956 747 432 268 183 72 55
28
EcoO109I rG'GnC_Cy
Cuts at: 504 930 504
Size: 426 3365
EcoRI G'AATT_C
Cuts at: 795 795
Size: 3791
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 670 1810 670
Size: 1140 2651
FspI TGC'GCA
Cuts at: 1572 1572
Size: 3791
HaeII r_GCGC'y
Cuts at: 1225 1473 2495 3474 1225
Size: 248 1022 979 1542
Fragments arranged by size:
1542 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 138 170 309 341 1633 1663 1665 1695 138
Size: 32 139 32 1292 30 2 30 2234
Fragments arranged by size:
2234 1292 139 32 32 30 30 2
HincII GTy'rAC
Cuts at: 830 830
Size: 3791
HindIII A'AGCT_T
Cuts at: 380 380
Size: 3791
KasI G'GCGC_C
Cuts at: 1221 1469 1221
Size: 248 3543
KpnI G_GTAC'C
Cuts at: 811 811
Size: 3791
MluI A'CGCG_T
Cuts at: 3224 3224
Size: 3791
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 254 2462 2646 254
Size: 2208 184 1399
Fragments arranged by size:
2208 1399 184
MscI TGG'CCA
Cuts at: 1552 1552
Size: 3791
MslI CAynn'nnrTG
Cuts at: 391 1780 1907 391
Size: 1389 127 2275
Fragments arranged by size:
2275 1389 127
MspA1I CmG'CkG
Cuts at: 902 1216 1576 2589 2834 902
Size: 314 360 1013 245 1859
Fragments arranged by size:
1859 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1972 1972
Size: 3791
NarI GG'CG_CC
Cuts at: 1222 1470 1222
Size: 248 3543
NcoI C'CATG_G
Cuts at: 491 491
Size: 3791
NgoMIV G'CCGG_C
Cuts at: 1970 1970
Size: 3791
NspI r_CATG'y
Cuts at: 844 1344 2251 844
Size: 500 907 2384
Fragments arranged by size:
2384 907 500
PciI A'CATG_T
Cuts at: 2247 2247
Size: 3791
PfoI T'CCnGG_A
Cuts at: 263 263
Size: 3791
PmeI GTTT'AAAC
Cuts at: 790 790
Size: 3791
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2930 2962 2930
Size: 32 3759
PstI C_TGCA'G
Cuts at: 838 1523 838
Size: 685 3106
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3791
PvuII CAG'CTG
Cuts at: 1216 1576 1216
Size: 360 3431
RsrII CG'GwC_CG
Cuts at: 1986 1986
Size: 3791
SacI G_AGCT'C
Cuts at: 201 805 201
Size: 604 3187
SalI G'TCGA_C
Cuts at: 828 828
Size: 3791
SapI GCTCTTCn'nnn_
Cuts at: 189 1814 2024 189
Size: 1625 210 1956
Fragments arranged by size:
1956 1625 210
SbfI CC_TGCA'GG
Cuts at: 838 838
Size: 3791
ScaI AGT'ACT
Cuts at: 3308 3308
Size: 3791
SfcI C'TryA_G
Cuts at: 33 741 834 1519 2512 2703 33
Size: 708 93 685 993 191 1121
Fragments arranged by size:
1121 993 708 685 191 93
SfoI GGC'GCC
Cuts at: 1223 1471 1223
Size: 248 3543
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3791
SmaI CCC'GGG
Cuts at: 813 813
Size: 3791
SmlI C'TyrA_G
Cuts at: 140 432 946 1861 2353 2615 140
Size: 292 514 915 492 262 1316
Fragments arranged by size:
1316 915 514 492 292 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3791
SphI G_CATG'C
Cuts at: 844 1344 844
Size: 500 3291
StyI C'CwwG_G
Cuts at: 491 925 1279 491
Size: 434 354 3003
Fragments arranged by size:
3003 434 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1374 1507 1966 2175 1374
Size: 133 459 209 2990
Fragments arranged by size:
2990 459 209 133
TatI w'GTAC_w
Cuts at: 3306 3306
Size: 3791
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3308 3340 3308
Size: 32 3759
Tth111I GACn'n_nGTC
Cuts at: 1588 1588
Size: 3791
XbaI T'CTAG_A
Cuts at: 822 822
Size: 3791
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 406 1286 406
Size: 880 2911
XmaI C'CCGG_G
Cuts at: 811 811
Size: 3791
XmnI GAAnn'nnTTC
Cuts at: 622 622
Size: 3791
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PfoI PmeI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BcgI BfrBI BglI BmgBI BmtI
BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI ZraI