Restriction map for
pF1KE0207
(Circular) (Six-base) MAPSORT of: pF1KE0207.seq Check: 6071 from: 1 to: 3944
pF1KE0207 3944 bp
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 364 364
Size: 3944
AccI GT'mk_AC
Cuts at: 982 982
Size: 3944
Acc65I G'GTAC_C
Cuts at: 960 960
Size: 3944
AclI AA'CG_TT
Cuts at: 3784 3784
Size: 3944
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1769 2201 2948 3216 1769
Size: 432 747 268 2497
Fragments arranged by size:
2497 747 432 268
AflIII A'CryG_T
Cuts at: 730 1296 2400 3377 730
Size: 566 1104 977 1297
Fragments arranged by size:
1297 1104 977 566
AgeI A'CCGG_T
Cuts at: 2332 2332
Size: 3944
AlwNI CAG_nnn'CTG
Cuts at: 781 1249 2816 781
Size: 468 1567 1909
Fragments arranged by size:
1909 1567 468
ApaI G_GGCC'C
Cuts at: 188 209 188
Size: 21 3923
ApaLI G'TGCA_C
Cuts at: 2714 2714
Size: 3944
ApoI r'AATT_y
Cuts at: 948 3297 3553 948
Size: 2349 256 1339
Fragments arranged by size:
2349 1339 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3944
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3944
AvaI C'yCGr_G
Cuts at: 208 265 964 208
Size: 57 699 3188
Fragments arranged by size:
3188 699 57
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 717 750 717
Size: 33 3911
BamHI G'GATC_C
Cuts at: 969 969
Size: 3944
BanI G'GyrC_C
Cuts at: 960 1374 1622 1657 960
Size: 414 248 35 3247
Fragments arranged by size:
3247 414 248 35
BanII G_rGCy'C
Cuts at: 149 188 209 891 958 1988 149
Size: 39 21 682 67 1030 2105
Fragments arranged by size:
2105 1030 682 67 39 21
BbeI G_GCGC'C
Cuts at: 1378 1626 1378
Size: 248 3696
BciVI GTATCCnnnnn_n'
Cuts at: 1842 2603 3944 1842
Size: 761 1341 1842
Fragments arranged by size:
1842 1341 761
BclI T'GATC_A
Cuts at: 350 1464 350
Size: 1114 2830
BfrBI ATG'CAT
Cuts at: 399 399
Size: 3944
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 3944
BglII A'GATC_T
Cuts at: 1459 3496 1459
Size: 2037 1907
BlpI GC'TnA_GC
Cuts at: 1056 1056
Size: 3944
Bme1580I G_kGCm'C
Cuts at: 188 209 345 1569 1662 2718 188
Size: 21 136 1224 93 1056 1414
Fragments arranged by size:
1414 1224 1056 136 93 21
BmrI ACTGGGnnnn_n'
Cuts at: 1156 1237 1572 3716 1156
Size: 81 335 2144 1384
Fragments arranged by size:
2144 1384 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2311 2311
Size: 3944
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1120 1999 2491 2789 1120
Size: 879 492 298 2275
Fragments arranged by size:
2275 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3648 3648
Size: 3944
BsaAI yAC'GTr
Cuts at: 733 1297 733
Size: 564 3380
BsaBI GATnn'nnATC
Cuts at: 6 1458 3495 6
Size: 1452 2037 455
Fragments arranged by size:
2037 1452 455
BsaHI Gr'CG_yC
Cuts at: 1375 1623 2325 3821 1375
Size: 248 702 1496 1498
Fragments arranged by size:
1498 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1133 1654 2332 2606 2753 3164 1133
Size: 521 678 274 147 411 1913
Fragments arranged by size:
1913 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 202 1369 2127 2704 3200 202
Size: 1167 758 577 496 946
Fragments arranged by size:
1167 946 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1486 1532 2740 104
Size: 1382 46 1208 1308
Fragments arranged by size:
1382 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 958 1736 1926 2718 958
Size: 778 190 792 2184
Fragments arranged by size:
2184 792 778 190
BsiWI C'GTAC_G
Cuts at: 239 239
Size: 3944
BsmI GAATG_Cn'
Cuts at: 456 456
Size: 3944
Bsp1286I G_dGCh'C
Cuts at: 149 188 209 345 891 958 1569 1662 1736
Size: 39 21 136 546 67 611 93 74
Cuts at: 1736 1926 1988 2718 149
Size: 190 62 730 1375
Fragments arranged by size:
1375 730 611 546 190 136 93 74
67 62 39 21
BspEI T'CCGG_A
Cuts at: 3164 3164
Size: 3944
BspHI T'CATG_A
Cuts at: 3120 3120
Size: 3944
BspMI ACCTGCnnnn'nnnn_
Cuts at: 364 994 1208 1510 1891 364
Size: 630 214 302 381 2417
Fragments arranged by size:
2417 630 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2236 2290 3774 3939 50
Size: 2186 54 1484 165 55
Fragments arranged by size:
2186 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 435 633 1856 435
Size: 198 1223 2523
Fragments arranged by size:
2523 1223 198
BsrFI r'CCGG_y
Cuts at: 1942 2123 2332 1942
Size: 181 209 3554
Fragments arranged by size:
3554 209 181
BssSI C'ACGA_G
Cuts at: 2215 2573 2215
Size: 358 3586
BstAPI GCAn_nnn'nTGC
Cuts at: 781 1321 781
Size: 540 3404
BstBI TT'CG_AA
Cuts at: 2305 2305
Size: 3944
BstYI r'GATC_y
Cuts at: 1 969 1436 1459 1794 2040 3052 3138 3150
Size: 968 467 23 335 246 1012 86 12
Cuts at: 3150 3496 1
Size: 346 449
Fragments arranged by size:
1012 968 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 608 107
Size: 501 3443
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1894 2075 2238 1894
Size: 181 163 3600
Fragments arranged by size:
3600 181 163
BtsI GCAGTG_nn'
Cuts at: 693 779 1187 693
Size: 86 408 3450
Fragments arranged by size:
3450 408 86
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 774 809 774
Size: 35 3909
DraI TTT'AAA
Cuts at: 943 943
Size: 3944
DrdI GACnn_nn'nnGTC
Cuts at: 590 1650 2508 590
Size: 1060 858 2026
Fragments arranged by size:
2026 1060 858
EaeI y'GGCC_r
Cuts at: 1529 1703 2094 2121 3466 1529
Size: 174 391 27 1345 2007
Fragments arranged by size:
2007 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1529 1529
Size: 3944
EarI CTCTTCn'nnn_
Cuts at: 1967 2177 1967
Size: 210 3734
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2152 2462 2608 3754 2152
Size: 310 146 1146 2342
Fragments arranged by size:
2342 1146 310 146
EcoICRI GAG'CTC
Cuts at: 956 956
Size: 3944
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1769 2201 2948 3216 1769
Size: 432 747 268 2497
Fragments arranged by size:
2497 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 184 297 568 1083 184
Size: 113 271 515 3045
Fragments arranged by size:
3045 515 271 113
EcoRI G'AATT_C
Cuts at: 948 948
Size: 3944
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1963 1963
Size: 3944
FspI TGC'GCA
Cuts at: 1725 1725
Size: 3944
HaeII r_GCGC'y
Cuts at: 1378 1626 2648 3627 1378
Size: 248 1022 979 1695
Fragments arranged by size:
1695 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1786 1816 1818 1848 1786
Size: 30 2 30 3882
Fragments arranged by size:
3882 30 30 2
HincII GTy'rAC
Cuts at: 983 983
Size: 3944
KasI G'GCGC_C
Cuts at: 1374 1622 1374
Size: 248 3696
KpnI G_GTAC'C
Cuts at: 964 964
Size: 3944
MluI A'CGCG_T
Cuts at: 3377 3377
Size: 3944
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2615 2799 2615
Size: 184 3760
MscI TGG'CCA
Cuts at: 1705 1705
Size: 3944
MslI CAynn'nnrTG
Cuts at: 382 404 1933 2060 382
Size: 22 1529 127 2266
Fragments arranged by size:
2266 1529 127 22
MspA1I CmG'CkG
Cuts at: 135 1055 1369 1729 2742 2987 135
Size: 920 314 360 1013 245 1092
Fragments arranged by size:
1092 1013 920 360 314 245
NaeI GCC'GGC
Cuts at: 2125 2125
Size: 3944
NarI GG'CG_CC
Cuts at: 1375 1623 1375
Size: 248 3696
NcoI C'CATG_G
Cuts at: 107 608 107
Size: 501 3443
NgoMIV G'CCGG_C
Cuts at: 2123 2123
Size: 3944
NsiI A_TGCA'T
Cuts at: 401 401
Size: 3944
NspI r_CATG'y
Cuts at: 409 533 817 997 1497 2404 409
Size: 124 284 180 500 907 1949
Fragments arranged by size:
1949 907 500 284 180 124
PasI CC'CwG_GG
Cuts at: 460 460
Size: 3944
PciI A'CATG_T
Cuts at: 2400 2400
Size: 3944
PflMI CCAn_nnn'nTGG
Cuts at: 609 609
Size: 3944
PfoI T'CCnGG_A
Cuts at: 579 579
Size: 3944
PmeI GTTT'AAAC
Cuts at: 943 943
Size: 3944
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3083 3115 3083
Size: 32 3912
PpuMI rG'GwC_Cy
Cuts at: 297 297
Size: 3944
PspOMI G'GGCC_C
Cuts at: 184 205 184
Size: 21 3923
PstI C_TGCA'G
Cuts at: 700 991 1676 700
Size: 291 685 2968
Fragments arranged by size:
2968 685 291
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3944
PvuII CAG'CTG
Cuts at: 1369 1729 1369
Size: 360 3584
RsrII CG'GwC_CG
Cuts at: 2139 2139
Size: 3944
SacI G_AGCT'C
Cuts at: 958 958
Size: 3944
SalI G'TCGA_C
Cuts at: 981 981
Size: 3944
SapI GCTCTTCn'nnn_
Cuts at: 1967 2177 1967
Size: 210 3734
SbfI CC_TGCA'GG
Cuts at: 991 991
Size: 3944
ScaI AGT'ACT
Cuts at: 3461 3461
Size: 3944
SfcI C'TryA_G
Cuts at: 33 696 987 1672 2665 2856 33
Size: 663 291 685 993 191 1121
Fragments arranged by size:
1121 993 685 663 291 191
SfoI GGC'GCC
Cuts at: 1376 1624 1376
Size: 248 3696
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3944
SmaI CCC'GGG
Cuts at: 210 966 210
Size: 756 3188
SmlI C'TyrA_G
Cuts at: 1099 2014 2506 2768 1099
Size: 915 492 262 2275
Fragments arranged by size:
2275 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3944
SphI G_CATG'C
Cuts at: 533 817 997 1497 533
Size: 284 180 500 2980
Fragments arranged by size:
2980 500 284 180
StuI AGG'CCT
Cuts at: 218 218
Size: 3944
StyI C'CwwG_G
Cuts at: 107 608 1078 1432 107
Size: 501 470 354 2619
Fragments arranged by size:
2619 501 470 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 601 1527 1660 2119 2328 601
Size: 926 133 459 209 2217
Fragments arranged by size:
2217 926 459 209 133
TatI w'GTAC_w
Cuts at: 3459 3459
Size: 3944
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3461 3493 3461
Size: 32 3912
Tth111I GACn'n_nGTC
Cuts at: 1741 1741
Size: 3944
XbaI T'CTAG_A
Cuts at: 975 975
Size: 3944
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1439 1439
Size: 3944
XmaI C'CCGG_G
Cuts at: 208 964 208
Size: 756 3188
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseYI BsiEI BsiHKAI BsiWI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BbsI BbvCI BcgI BmgBI BmtI BpmI Bpu10I
BsaXI BseRI BsgI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI PacI
PmlI PshAI PsiI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI XmnI
ZraI