Restriction map for
pF1KE0209
(Circular) (Six-base) MAPSORT of: pF1KE0209.seq Check: 5434 from: 1 to: 4022
pF1KE0209 4022 bp
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AccI GT'mk_AC
Cuts at: 1060 1060
Size: 4022
Acc65I G'GTAC_C
Cuts at: 1038 1038
Size: 4022
AclI AA'CG_TT
Cuts at: 3862 3862
Size: 4022
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1847 2279 3026 3294 1847
Size: 432 747 268 2575
Fragments arranged by size:
2575 747 432 268
AfeI AGC'GCT
Cuts at: 405 405
Size: 4022
AflIII A'CryG_T
Cuts at: 1374 2478 3455 1374
Size: 1104 977 1941
Fragments arranged by size:
1941 1104 977
AgeI A'CCGG_T
Cuts at: 2410 2410
Size: 4022
AlwNI CAG_nnn'CTG
Cuts at: 1327 2894 1327
Size: 1567 2455
ApaI G_GGCC'C
Cuts at: 390 390
Size: 4022
ApaLI G'TGCA_C
Cuts at: 2792 2792
Size: 4022
ApoI r'AATT_y
Cuts at: 631 888 1026 3375 3631 631
Size: 257 138 2349 256 1022
Fragments arranged by size:
2349 1022 257 256 138
AseI AT'TA_AT
Cuts at: 20 20
Size: 4022
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4022
AvaI C'yCGr_G
Cuts at: 1042 1042
Size: 4022
BamHI G'GATC_C
Cuts at: 1047 1047
Size: 4022
BanI G'GyrC_C
Cuts at: 961 1038 1452 1700 1735 961
Size: 77 414 248 35 3248
Fragments arranged by size:
3248 414 248 77 35
BanII G_rGCy'C
Cuts at: 390 1036 2066 390
Size: 646 1030 2346
Fragments arranged by size:
2346 1030 646
BbeI G_GCGC'C
Cuts at: 965 1456 1704 965
Size: 491 248 3283
Fragments arranged by size:
3283 491 248
BbvCI CC'TCA_GC
Cuts at: 925 925
Size: 4022
BciVI GTATCCnnnnn_n'
Cuts at: 1920 2681 4022 1920
Size: 761 1341 1920
Fragments arranged by size:
1920 1341 761
BclI T'GATC_A
Cuts at: 1542 1542
Size: 4022
BglI GCCn_nnn'nGGC
Cuts at: 129 129
Size: 4022
BglII A'GATC_T
Cuts at: 1537 3574 1537
Size: 2037 1985
BlpI GC'TnA_GC
Cuts at: 1134 1134
Size: 4022
Bme1580I G_kGCm'C
Cuts at: 390 1647 1740 2796 390
Size: 1257 93 1056 1616
Fragments arranged by size:
1616 1257 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1234 1315 1650 3794 1234
Size: 81 335 2144 1462
Fragments arranged by size:
2144 1462 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2389 2389
Size: 4022
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 690 690
Size: 4022
Bpu10I CC'TnA_GC
Cuts at: 925 925
Size: 4022
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1198 2077 2569 2867 1198
Size: 879 492 298 2353
Fragments arranged by size:
2353 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 556 3726 556
Size: 3170 852
BsaAI yAC'GTr
Cuts at: 1375 1375
Size: 4022
BsaBI GATnn'nnATC
Cuts at: 6 424 1536 3573 6
Size: 418 1112 2037 455
Fragments arranged by size:
2037 1112 455 418
BsaHI Gr'CG_yC
Cuts at: 962 1453 1701 2403 3899 962
Size: 491 248 702 1496 1085
Fragments arranged by size:
1496 1085 702 491 248
BsaWI w'CCGG_w
Cuts at: 653 784 884 1211 1732 2410 2684 2831 3242
Size: 131 100 327 521 678 274 147 411
Cuts at: 3242 653
Size: 1433
Fragments arranged by size:
1433 678 521 411 327 274 147 131
100
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 361 391 481 511 673 703 361
Size: 30 90 30 162 30 3680
Fragments arranged by size:
3680 162 90 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1000 1000
Size: 4022
BseYI C'CCAG_C
Cuts at: 240 282 302 383 844 1447 2205 2782 3278
Size: 42 20 81 461 603 758 577 496
Cuts at: 3278 240
Size: 984
Fragments arranged by size:
984 758 603 577 496 461 81 42
20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 487 487
Size: 4022
BsiEI CG_ry'CG
Cuts at: 104 136 1564 1610 2818 104
Size: 32 1428 46 1208 1308
Fragments arranged by size:
1428 1308 1208 46 32
BsiHKAI G_wGCw'C
Cuts at: 992 1036 1814 2004 2796 992
Size: 44 778 190 792 2218
Fragments arranged by size:
2218 792 778 190 44
Bsp1286I G_dGCh'C
Cuts at: 390 992 1036 1647 1740 1814 2004 2066 2796
Size: 602 44 611 93 74 190 62 730
Cuts at: 2796 390
Size: 1616
Fragments arranged by size:
1616 730 611 602 190 93 74 62
44
BspEI T'CCGG_A
Cuts at: 653 884 3242 653
Size: 231 2358 1433
Fragments arranged by size:
2358 1433 231
BspHI T'CATG_A
Cuts at: 267 3198 267
Size: 2931 1091
BspMI ACCTGCnnnn'nnnn_
Cuts at: 211 574 1072 1286 1588 1969 211
Size: 363 498 214 302 381 2264
Fragments arranged by size:
2264 498 381 363 302 214
BsrBI CCG'CTC
Cuts at: 50 2314 2368 3852 4017 50
Size: 2264 54 1484 165 55
Fragments arranged by size:
2264 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 321 633 1934 321
Size: 312 1301 2409
Fragments arranged by size:
2409 1301 312
BsrFI r'CCGG_y
Cuts at: 209 2020 2201 2410 209
Size: 1811 181 209 1821
Fragments arranged by size:
1821 1811 209 181
BsrGI T'GTAC_A
Cuts at: 994 994
Size: 4022
BssSI C'ACGA_G
Cuts at: 148 2293 2651 148
Size: 2145 358 1519
Fragments arranged by size:
2145 1519 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1399 1399
Size: 4022
BstBI TT'CG_AA
Cuts at: 2383 2383
Size: 4022
BstXI CCAn_nnnn'nTGG
Cuts at: 408 408
Size: 4022
BstYI r'GATC_y
Cuts at: 1 288 1047 1514 1537 1872 2118 3130 3216
Size: 287 759 467 23 335 246 1012 86
Cuts at: 3216 3228 3574 1
Size: 12 346 449
Fragments arranged by size:
1012 759 467 449 346 335 287 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 904 107
Size: 797 3225
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1972 2153 2316 1972
Size: 181 163 3678
Fragments arranged by size:
3678 181 163
BtsI GCAGTG_nn'
Cuts at: 1265 1265
Size: 4022
DraI TTT'AAA
Cuts at: 1021 1021
Size: 4022
DrdI GACnn_nn'nnGTC
Cuts at: 1728 2586 1728
Size: 858 3164
EaeI y'GGCC_r
Cuts at: 133 1607 1781 2172 2199 3544 133
Size: 1474 174 391 27 1345 611
Fragments arranged by size:
1474 1345 611 391 174 27
EagI C'GGCC_G
Cuts at: 133 1607 133
Size: 1474 2548
EarI CTCTTCn'nnn_
Cuts at: 2045 2255 2045
Size: 210 3812
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 178 427 2230 2540 2686 3832 178
Size: 249 1803 310 146 1146 368
Fragments arranged by size:
1803 1146 368 310 249 146
EcoICRI GAG'CTC
Cuts at: 1034 1034
Size: 4022
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 690 1847 2279 3026 3294 690
Size: 1157 432 747 268 1418
Fragments arranged by size:
1418 1157 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1005 1161 1005
Size: 156 3866
EcoRI G'AATT_C
Cuts at: 631 888 1026 631
Size: 257 138 3627
Fragments arranged by size:
3627 257 138
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2041 2041
Size: 4022
FspI TGC'GCA
Cuts at: 1803 1803
Size: 4022
HaeII r_GCGC'y
Cuts at: 407 965 1456 1704 2726 3705 407
Size: 558 491 248 1022 979 724
Fragments arranged by size:
1022 979 724 558 491 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 262 294 673 705 1864 1894 1896 1926 262
Size: 32 379 32 1159 30 2 30 2358
Fragments arranged by size:
2358 1159 379 32 32 30 30 2
HincII GTy'rAC
Cuts at: 330 1061 330
Size: 731 3291
KasI G'GCGC_C
Cuts at: 961 1452 1700 961
Size: 491 248 3283
Fragments arranged by size:
3283 491 248
KpnI G_GTAC'C
Cuts at: 1042 1042
Size: 4022
MluI A'CGCG_T
Cuts at: 3455 3455
Size: 4022
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2693 2877 2693
Size: 184 3838
MscI TGG'CCA
Cuts at: 1783 1783
Size: 4022
MslI CAynn'nnrTG
Cuts at: 2011 2138 2011
Size: 127 3895
MspA1I CmG'CkG
Cuts at: 240 286 746 1133 1447 1807 2820 3065 240
Size: 46 460 387 314 360 1013 245 1197
Fragments arranged by size:
1197 1013 460 387 360 314 245 46
NaeI GCC'GGC
Cuts at: 2203 2203
Size: 4022
NarI GG'CG_CC
Cuts at: 962 1453 1701 962
Size: 491 248 3283
Fragments arranged by size:
3283 491 248
NcoI C'CATG_G
Cuts at: 107 904 107
Size: 797 3225
NgoMIV G'CCGG_C
Cuts at: 2201 2201
Size: 4022
NotI GC'GGCC_GC
Cuts at: 133 133
Size: 4022
NspI r_CATG'y
Cuts at: 626 1075 1575 2482 626
Size: 449 500 907 2166
Fragments arranged by size:
2166 907 500 449
PciI A'CATG_T
Cuts at: 2478 2478
Size: 4022
PflMI CCAn_nnn'nTGG
Cuts at: 302 302
Size: 4022
PmeI GTTT'AAAC
Cuts at: 1021 1021
Size: 4022
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3161 3193 3161
Size: 32 3990
PpuMI rG'GwC_Cy
Cuts at: 1005 1005
Size: 4022
PspOMI G'GGCC_C
Cuts at: 386 386
Size: 4022
PstI C_TGCA'G
Cuts at: 1069 1754 1069
Size: 685 3337
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4022
PvuII CAG'CTG
Cuts at: 746 1447 1807 746
Size: 701 360 2961
Fragments arranged by size:
2961 701 360
RsrII CG'GwC_CG
Cuts at: 2217 2217
Size: 4022
SacI G_AGCT'C
Cuts at: 1036 1036
Size: 4022
SalI G'TCGA_C
Cuts at: 1059 1059
Size: 4022
SanDI GG'GwC_CC
Cuts at: 1005 1005
Size: 4022
SapI GCTCTTCn'nnn_
Cuts at: 2045 2255 2045
Size: 210 3812
SbfI CC_TGCA'GG
Cuts at: 1069 1069
Size: 4022
ScaI AGT'ACT
Cuts at: 874 3539 874
Size: 2665 1357
SfcI C'TryA_G
Cuts at: 33 1065 1750 2743 2934 33
Size: 1032 685 993 191 1121
Fragments arranged by size:
1121 1032 993 685 191
SfoI GGC'GCC
Cuts at: 963 1454 1702 963
Size: 491 248 3283
Fragments arranged by size:
3283 491 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4022
SmaI CCC'GGG
Cuts at: 1044 1044
Size: 4022
SmlI C'TyrA_G
Cuts at: 1177 2092 2584 2846 1177
Size: 915 492 262 2353
Fragments arranged by size:
2353 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4022
SphI G_CATG'C
Cuts at: 1075 1575 1075
Size: 500 3522
StyI C'CwwG_G
Cuts at: 107 584 904 1156 1510 107
Size: 477 320 252 354 2619
Fragments arranged by size:
2619 477 354 320 252
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 836 1605 1738 2197 2406 836
Size: 769 133 459 209 2452
Fragments arranged by size:
2452 769 459 209 133
TatI w'GTAC_w
Cuts at: 872 994 3537 872
Size: 122 2543 1357
Fragments arranged by size:
2543 1357 122
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 580 612 3539 3571 580
Size: 32 2927 32 1031
Fragments arranged by size:
2927 1031 32 32
Tth111I GACn'n_nGTC
Cuts at: 1819 1819
Size: 4022
XbaI T'CTAG_A
Cuts at: 1053 1053
Size: 4022
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1517 1517
Size: 4022
XmaI C'CCGG_G
Cuts at: 1042 1042
Size: 4022
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbvCI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NotI NspI PciI PflMI PmeI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbsI BcgI BfrBI BmgBI BmtI BsiWI
BsmI BsmBI BssHII BstEII BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NruI NsiI PacI PasI PfoI PmlI
PshAI PsiI PspXI PsrI SacII SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI