Restriction map for
pF1KE0210
(Circular) (Six-base) MAPSORT of: pF1KE0210.seq Check: 3679 from: 1 to: 3923
pF1KE0210 3923 bp
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AccI GT'mk_AC
Cuts at: 961 961
Size: 3923
Acc65I G'GTAC_C
Cuts at: 939 939
Size: 3923
AclI AA'CG_TT
Cuts at: 3763 3763
Size: 3923
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1748 2180 2927 3195 1748
Size: 432 747 268 2476
Fragments arranged by size:
2476 747 432 268
AfeI AGC'GCT
Cuts at: 404 404
Size: 3923
AflII C'TTAA_G
Cuts at: 745 745
Size: 3923
AflIII A'CryG_T
Cuts at: 1275 2379 3356 1275
Size: 1104 977 1842
Fragments arranged by size:
1842 1104 977
AgeI A'CCGG_T
Cuts at: 2311 2311
Size: 3923
AlwNI CAG_nnn'CTG
Cuts at: 208 229 232 1228 2795 208
Size: 21 3 996 1567 1336
Fragments arranged by size:
1567 1336 996 21 3
ApaLI G'TGCA_C
Cuts at: 2693 2693
Size: 3923
ApoI r'AATT_y
Cuts at: 311 328 517 927 3276 3532 311
Size: 17 189 410 2349 256 702
Fragments arranged by size:
2349 702 410 256 189 17
AseI AT'TA_AT
Cuts at: 20 20
Size: 3923
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3923
AvaI C'yCGr_G
Cuts at: 943 943
Size: 3923
BamHI G'GATC_C
Cuts at: 948 948
Size: 3923
BanI G'GyrC_C
Cuts at: 939 1353 1601 1636 939
Size: 414 248 35 3226
Fragments arranged by size:
3226 414 248 35
BanII G_rGCy'C
Cuts at: 937 1967 937
Size: 1030 2893
BbeI G_GCGC'C
Cuts at: 1357 1605 1357
Size: 248 3675
BbvCI CC'TCA_GC
Cuts at: 415 415
Size: 3923
BciVI GTATCCnnnnn_n'
Cuts at: 1821 2582 3923 1821
Size: 761 1341 1821
Fragments arranged by size:
1821 1341 761
BclI T'GATC_A
Cuts at: 1443 1443
Size: 3923
BfrBI ATG'CAT
Cuts at: 306 306
Size: 3923
BglII A'GATC_T
Cuts at: 775 1438 3475 775
Size: 663 2037 1223
Fragments arranged by size:
2037 1223 663
BlpI GC'TnA_GC
Cuts at: 1035 1035
Size: 3923
Bme1580I G_kGCm'C
Cuts at: 451 1548 1641 2697 451
Size: 1097 93 1056 1677
Fragments arranged by size:
1677 1097 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1135 1216 1551 3695 1135
Size: 81 335 2144 1363
Fragments arranged by size:
2144 1363 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2290 2290
Size: 3923
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 258 258
Size: 3923
Bpu10I CC'TnA_GC
Cuts at: 415 415
Size: 3923
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 345 1099 1978 2470 2768 345
Size: 754 879 492 298 1500
Fragments arranged by size:
1500 879 754 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3627 3627
Size: 3923
BsaAI yAC'GTr
Cuts at: 1276 1276
Size: 3923
BsaBI GATnn'nnATC
Cuts at: 6 1437 3474 6
Size: 1431 2037 455
Fragments arranged by size:
2037 1431 455
BsaHI Gr'CG_yC
Cuts at: 1354 1602 2304 3800 1354
Size: 248 702 1496 1477
Fragments arranged by size:
1496 1477 702 248
BsaWI w'CCGG_w
Cuts at: 1112 1633 2311 2585 2732 3143 1112
Size: 521 678 274 147 411 1892
Fragments arranged by size:
1892 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 485 485
Size: 3923
BseYI C'CCAG_C
Cuts at: 1348 2106 2683 3179 1348
Size: 758 577 496 2092
Fragments arranged by size:
2092 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1465 1511 2719 104
Size: 1361 46 1208 1308
Fragments arranged by size:
1361 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 937 1715 1905 2697 937
Size: 778 190 792 2163
Fragments arranged by size:
2163 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 469 469
Size: 3923
Bsp1286I G_dGCh'C
Cuts at: 451 937 1548 1641 1715 1905 1967 2697 451
Size: 486 611 93 74 190 62 730 1677
Fragments arranged by size:
1677 730 611 486 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3143 3143
Size: 3923
BspHI T'CATG_A
Cuts at: 284 3099 284
Size: 2815 1108
BspMI ACCTGCnnnn'nnnn_
Cuts at: 973 1187 1489 1870 973
Size: 214 302 381 3026
Fragments arranged by size:
3026 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2215 2269 3753 3918 50
Size: 2165 54 1484 165 55
Fragments arranged by size:
2165 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1835 1835
Size: 3923
BsrFI r'CCGG_y
Cuts at: 1921 2102 2311 1921
Size: 181 209 3533
Fragments arranged by size:
3533 209 181
BssSI C'ACGA_G
Cuts at: 2194 2552 2194
Size: 358 3565
BstAPI GCAn_nnn'nTGC
Cuts at: 229 303 332 1300 229
Size: 74 29 968 2852
Fragments arranged by size:
2852 968 74 29
BstBI TT'CG_AA
Cuts at: 2284 2284
Size: 3923
BstYI r'GATC_y
Cuts at: 1 574 775 948 1415 1438 1773 2019 3031
Size: 573 201 173 467 23 335 246 1012
Cuts at: 3031 3117 3129 3475 1
Size: 86 12 346 449
Fragments arranged by size:
1012 573 467 449 346 335 246 201
173 86 23 12
BtgI C'CryG_G
Cuts at: 107 422 107
Size: 315 3608
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1873 2054 2217 1873
Size: 181 163 3579
Fragments arranged by size:
3579 181 163
BtsI GCAGTG_nn'
Cuts at: 1166 1166
Size: 3923
DraI TTT'AAA
Cuts at: 216 922 216
Size: 706 3217
DrdI GACnn_nn'nnGTC
Cuts at: 1629 2487 1629
Size: 858 3065
EaeI y'GGCC_r
Cuts at: 425 1508 1682 2073 2100 3445 425
Size: 1083 174 391 27 1345 903
Fragments arranged by size:
1345 1083 903 391 174 27
EagI C'GGCC_G
Cuts at: 1508 1508
Size: 3923
EarI CTCTTCn'nnn_
Cuts at: 437 837 1946 2156 437
Size: 400 1109 210 2204
Fragments arranged by size:
2204 1109 400 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2131 2441 2587 3733 2131
Size: 310 146 1146 2321
Fragments arranged by size:
2321 1146 310 146
EcoICRI GAG'CTC
Cuts at: 935 935
Size: 3923
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 258 1748 2180 2927 3195 258
Size: 1490 432 747 268 986
Fragments arranged by size:
1490 986 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1062 1062
Size: 3923
EcoRI G'AATT_C
Cuts at: 927 927
Size: 3923
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 771 1942 771
Size: 1171 2752
FspI TGC'GCA
Cuts at: 1704 1704
Size: 3923
HaeII r_GCGC'y
Cuts at: 406 1357 1605 2627 3606 406
Size: 951 248 1022 979 723
Fragments arranged by size:
1022 979 951 723 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 897 929 1765 1795 1797 1827 897
Size: 32 836 30 2 30 2993
Fragments arranged by size:
2993 836 32 30 30 2
HincII GTy'rAC
Cuts at: 529 962 529
Size: 433 3490
KasI G'GCGC_C
Cuts at: 1353 1601 1353
Size: 248 3675
KpnI G_GTAC'C
Cuts at: 943 943
Size: 3923
MluI A'CGCG_T
Cuts at: 3356 3356
Size: 3923
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2594 2778 2594
Size: 184 3739
MscI TGG'CCA
Cuts at: 427 1684 427
Size: 1257 2666
MslI CAynn'nnrTG
Cuts at: 896 1912 2039 896
Size: 1016 127 2780
Fragments arranged by size:
2780 1016 127
MspA1I CmG'CkG
Cuts at: 229 659 1034 1348 1708 2721 2966 229
Size: 430 375 314 360 1013 245 1186
Fragments arranged by size:
1186 1013 430 375 360 314 245
NaeI GCC'GGC
Cuts at: 2104 2104
Size: 3923
NarI GG'CG_CC
Cuts at: 1354 1602 1354
Size: 248 3675
NcoI C'CATG_G
Cuts at: 107 422 107
Size: 315 3608
NgoMIV G'CCGG_C
Cuts at: 2102 2102
Size: 3923
NsiI A_TGCA'T
Cuts at: 308 308
Size: 3923
NspI r_CATG'y
Cuts at: 976 1476 2383 976
Size: 500 907 2516
Fragments arranged by size:
2516 907 500
PciI A'CATG_T
Cuts at: 2379 2379
Size: 3923
PfoI T'CCnGG_A
Cuts at: 666 666
Size: 3923
PmeI GTTT'AAAC
Cuts at: 922 922
Size: 3923
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3062 3094 3062
Size: 32 3891
PshAI GACnn'nnGTC
Cuts at: 130 130
Size: 3923
PstI C_TGCA'G
Cuts at: 970 1655 970
Size: 685 3238
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3923
PvuII CAG'CTG
Cuts at: 229 1348 1708 229
Size: 1119 360 2444
Fragments arranged by size:
2444 1119 360
RsrII CG'GwC_CG
Cuts at: 2118 2118
Size: 3923
SacI G_AGCT'C
Cuts at: 937 937
Size: 3923
SalI G'TCGA_C
Cuts at: 960 960
Size: 3923
SapI GCTCTTCn'nnn_
Cuts at: 1946 2156 1946
Size: 210 3713
SbfI CC_TGCA'GG
Cuts at: 970 970
Size: 3923
ScaI AGT'ACT
Cuts at: 3440 3440
Size: 3923
SexAI A'CCwGG_T
Cuts at: 485 485
Size: 3923
SfcI C'TryA_G
Cuts at: 33 883 966 1651 2644 2835 33
Size: 850 83 685 993 191 1121
Fragments arranged by size:
1121 993 850 685 191 83
SfoI GGC'GCC
Cuts at: 1355 1603 1355
Size: 248 3675
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3923
SmaI CCC'GGG
Cuts at: 945 945
Size: 3923
SmlI C'TyrA_G
Cuts at: 360 745 1078 1993 2485 2747 360
Size: 385 333 915 492 262 1536
Fragments arranged by size:
1536 915 492 385 333 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3923
SphI G_CATG'C
Cuts at: 976 1476 976
Size: 500 3423
StyI C'CwwG_G
Cuts at: 107 174 422 570 1057 1411 107
Size: 67 248 148 487 354 2619
Fragments arranged by size:
2619 487 354 248 148 67
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1506 1639 2098 2307 1506
Size: 133 459 209 3122
Fragments arranged by size:
3122 459 209 133
TatI w'GTAC_w
Cuts at: 3438 3438
Size: 3923
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3440 3472 3440
Size: 32 3891
Tth111I GACn'n_nGTC
Cuts at: 1720 1720
Size: 3923
XbaI T'CTAG_A
Cuts at: 954 954
Size: 3923
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1418 1418
Size: 3923
XmaI C'CCGG_G
Cuts at: 943 943
Size: 3923
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbvCI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NsiI NspI PciI PfoI PmeI PpiI PshAI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AhdI AleI AlfI AloI ApaI AscI
AvrII BaeI BbsI BcgI BglI BmgBI BmtI BsaXI
BsgI BsiWI BsmI BsrGI BssHII BstEII BstXI BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI PacI
PasI PflMI PmlI PpuMI PsiI PspOMI PspXI PsrI
SacII SanDI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI