Restriction map for
pF1KE0211
(Circular) (Six-base) MAPSORT of: pF1KE0211.seq Check: 9616 from: 1 to: 3371
pF1KE0211 3371 bp
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 155 155
Size: 3371
AccI GT'mk_AC
Cuts at: 409 409
Size: 3371
Acc65I G'GTAC_C
Cuts at: 387 387
Size: 3371
AclI AA'CG_TT
Cuts at: 365 3211 365
Size: 2846 525
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 310 1196 1628 2375 2643 310
Size: 886 432 747 268 1038
Fragments arranged by size:
1038 886 747 432 268
AflIII A'CryG_T
Cuts at: 723 1827 2804 723
Size: 1104 977 1290
Fragments arranged by size:
1290 1104 977
AgeI A'CCGG_T
Cuts at: 1759 1759
Size: 3371
AlwNI CAG_nnn'CTG
Cuts at: 676 2243 676
Size: 1567 1804
ApaLI G'TGCA_C
Cuts at: 2141 2141
Size: 3371
ApoI r'AATT_y
Cuts at: 375 2724 2980 375
Size: 2349 256 766
Fragments arranged by size:
2349 766 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3371
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3371
AvaI C'yCGr_G
Cuts at: 355 391 355
Size: 36 3335
BamHI G'GATC_C
Cuts at: 396 396
Size: 3371
BanI G'GyrC_C
Cuts at: 387 801 1049 1084 387
Size: 414 248 35 2674
Fragments arranged by size:
2674 414 248 35
BanII G_rGCy'C
Cuts at: 385 1415 385
Size: 1030 2341
BbeI G_GCGC'C
Cuts at: 805 1053 805
Size: 248 3123
BciVI GTATCCnnnnn_n'
Cuts at: 1269 2030 3371 1269
Size: 761 1341 1269
Fragments arranged by size:
1341 1269 761
BclI T'GATC_A
Cuts at: 891 891
Size: 3371
BglII A'GATC_T
Cuts at: 886 2923 886
Size: 2037 1334
BlpI GC'TnA_GC
Cuts at: 483 483
Size: 3371
Bme1580I G_kGCm'C
Cuts at: 996 1089 2145 996
Size: 93 1056 2222
Fragments arranged by size:
2222 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 583 664 999 3143 583
Size: 81 335 2144 811
Fragments arranged by size:
2144 811 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1738 1738
Size: 3371
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 160 160
Size: 3371
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 547 1426 1918 2216 547
Size: 879 492 298 1702
Fragments arranged by size:
1702 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3075 3075
Size: 3371
BsaAI yAC'GTr
Cuts at: 724 724
Size: 3371
BsaBI GATnn'nnATC
Cuts at: 6 885 2922 6
Size: 879 2037 455
Fragments arranged by size:
2037 879 455
BsaHI Gr'CG_yC
Cuts at: 802 1050 1752 3248 802
Size: 248 702 1496 925
Fragments arranged by size:
1496 925 702 248
BsaWI w'CCGG_w
Cuts at: 560 1081 1759 2033 2180 2591 560
Size: 521 678 274 147 411 1340
Fragments arranged by size:
1340 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 373 373
Size: 3371
BseYI C'CCAG_C
Cuts at: 796 1554 2131 2627 796
Size: 758 577 496 1540
Fragments arranged by size:
1540 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 913 959 2167 104
Size: 809 46 1208 1308
Fragments arranged by size:
1308 1208 809 46
BsiHKAI G_wGCw'C
Cuts at: 146 385 1163 1353 2145 146
Size: 239 778 190 792 1372
Fragments arranged by size:
1372 792 778 239 190
Bsp1286I G_dGCh'C
Cuts at: 146 385 996 1089 1163 1353 1415 2145 146
Size: 239 611 93 74 190 62 730 1372
Fragments arranged by size:
1372 730 611 239 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2591 2591
Size: 3371
BspHI T'CATG_A
Cuts at: 2547 2547
Size: 3371
BspMI ACCTGCnnnn'nnnn_
Cuts at: 155 421 635 937 1318 155
Size: 266 214 302 381 2208
Fragments arranged by size:
2208 381 302 266 214
BsrBI CCG'CTC
Cuts at: 50 1663 1717 3201 3366 50
Size: 1613 54 1484 165 55
Fragments arranged by size:
1613 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 269 1283 269
Size: 1014 2357
BsrFI r'CCGG_y
Cuts at: 1369 1550 1759 1369
Size: 181 209 2981
Fragments arranged by size:
2981 209 181
BssSI C'ACGA_G
Cuts at: 1642 2000 1642
Size: 358 3013
BstAPI GCAn_nnn'nTGC
Cuts at: 748 748
Size: 3371
BstBI TT'CG_AA
Cuts at: 1732 1732
Size: 3371
BstYI r'GATC_y
Cuts at: 1 396 863 886 1221 1467 2479 2565 2577
Size: 395 467 23 335 246 1012 86 12
Cuts at: 2577 2923 1
Size: 346 449
Fragments arranged by size:
1012 467 449 395 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1321 1502 1665 1321
Size: 181 163 3027
Fragments arranged by size:
3027 181 163
BtsI GCAGTG_nn'
Cuts at: 614 614
Size: 3371
DraI TTT'AAA
Cuts at: 370 370
Size: 3371
DraIII CAC_nnn'GTG
Cuts at: 191 191
Size: 3371
DrdI GACnn_nn'nnGTC
Cuts at: 1077 1935 1077
Size: 858 2513
EaeI y'GGCC_r
Cuts at: 956 1130 1521 1548 2893 956
Size: 174 391 27 1345 1434
Fragments arranged by size:
1434 1345 391 174 27
EagI C'GGCC_G
Cuts at: 956 956
Size: 3371
EarI CTCTTCn'nnn_
Cuts at: 1394 1604 1394
Size: 210 3161
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1579 1889 2035 3181 1579
Size: 310 146 1146 1769
Fragments arranged by size:
1769 1146 310 146
EcoICRI GAG'CTC
Cuts at: 383 383
Size: 3371
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 160 310 1196 1628 2375 2643 160
Size: 150 886 432 747 268 888
Fragments arranged by size:
888 886 747 432 268 150
EcoO109I rG'GnC_Cy
Cuts at: 510 510
Size: 3371
EcoRI G'AATT_C
Cuts at: 375 375
Size: 3371
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1390 1390
Size: 3371
FspI TGC'GCA
Cuts at: 1152 1152
Size: 3371
HaeII r_GCGC'y
Cuts at: 805 1053 2075 3054 805
Size: 248 1022 979 1122
Fragments arranged by size:
1122 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1213 1243 1245 1275 1213
Size: 30 2 30 3309
Fragments arranged by size:
3309 30 30 2
HincII GTy'rAC
Cuts at: 410 410
Size: 3371
KasI G'GCGC_C
Cuts at: 801 1049 801
Size: 248 3123
KpnI G_GTAC'C
Cuts at: 391 391
Size: 3371
MluI A'CGCG_T
Cuts at: 2804 2804
Size: 3371
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2042 2226 2042
Size: 184 3187
MscI TGG'CCA
Cuts at: 1132 1132
Size: 3371
MslI CAynn'nnrTG
Cuts at: 1360 1487 1360
Size: 127 3244
MspA1I CmG'CkG
Cuts at: 138 482 796 1156 2169 2414 138
Size: 344 314 360 1013 245 1095
Fragments arranged by size:
1095 1013 360 344 314 245
NaeI GCC'GGC
Cuts at: 1552 1552
Size: 3371
NarI GG'CG_CC
Cuts at: 802 1050 802
Size: 248 3123
NgoMIV G'CCGG_C
Cuts at: 1550 1550
Size: 3371
NspI r_CATG'y
Cuts at: 424 924 1831 424
Size: 500 907 1964
Fragments arranged by size:
1964 907 500
PciI A'CATG_T
Cuts at: 1827 1827
Size: 3371
PflMI CCAn_nnn'nTGG
Cuts at: 191 191
Size: 3371
PmeI GTTT'AAAC
Cuts at: 370 370
Size: 3371
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2510 2542 2510
Size: 32 3339
PstI C_TGCA'G
Cuts at: 152 418 1103 152
Size: 266 685 2420
Fragments arranged by size:
2420 685 266
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3371
PvuII CAG'CTG
Cuts at: 138 796 1156 138
Size: 658 360 2353
Fragments arranged by size:
2353 658 360
RsrII CG'GwC_CG
Cuts at: 1566 1566
Size: 3371
SacI G_AGCT'C
Cuts at: 385 385
Size: 3371
SalI G'TCGA_C
Cuts at: 408 408
Size: 3371
SapI GCTCTTCn'nnn_
Cuts at: 1394 1604 1394
Size: 210 3161
SbfI CC_TGCA'GG
Cuts at: 418 418
Size: 3371
ScaI AGT'ACT
Cuts at: 2888 2888
Size: 3371
SfcI C'TryA_G
Cuts at: 33 148 414 1099 2092 2283 33
Size: 115 266 685 993 191 1121
Fragments arranged by size:
1121 993 685 266 191 115
SfoI GGC'GCC
Cuts at: 803 1051 803
Size: 248 3123
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3371
SmaI CCC'GGG
Cuts at: 393 393
Size: 3371
SmlI C'TyrA_G
Cuts at: 526 1441 1933 2195 526
Size: 915 492 262 1702
Fragments arranged by size:
1702 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3371
SphI G_CATG'C
Cuts at: 424 924 424
Size: 500 2871
StyI C'CwwG_G
Cuts at: 505 859 505
Size: 354 3017
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 954 1087 1546 1755 954
Size: 133 459 209 2570
Fragments arranged by size:
2570 459 209 133
TatI w'GTAC_w
Cuts at: 158 2886 158
Size: 2728 643
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 136 168 2888 2920 136
Size: 32 2720 32 587
Fragments arranged by size:
2720 587 32 32
Tth111I GACn'n_nGTC
Cuts at: 1168 1168
Size: 3371
XbaI T'CTAG_A
Cuts at: 402 402
Size: 3371
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 866 866
Size: 3371
XmaI C'CCGG_G
Cuts at: 391 391
Size: 3371
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PciI PflMI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BsaXI BsgI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I Bsu36I BtgI ClaI
CspCI EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI