Restriction map for
pF1KE0212
(Circular) (Six-base) MAPSORT of: pF1KE0212.seq Check: 6354 from: 1 to: 3296
pF1KE0212 3296 bp
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AatII G_ACGT'C
Cuts at: 268 268
Size: 3296
AccI GT'mk_AC
Cuts at: 334 334
Size: 3296
Acc65I G'GTAC_C
Cuts at: 312 312
Size: 3296
AclI AA'CG_TT
Cuts at: 3136 3136
Size: 3296
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1121 1553 2300 2568 1121
Size: 432 747 268 1849
Fragments arranged by size:
1849 747 432 268
AflIII A'CryG_T
Cuts at: 648 1752 2729 648
Size: 1104 977 1215
Fragments arranged by size:
1215 1104 977
AgeI A'CCGG_T
Cuts at: 1684 1684
Size: 3296
AlwNI CAG_nnn'CTG
Cuts at: 284 601 2168 284
Size: 317 1567 1412
Fragments arranged by size:
1567 1412 317
ApaLI G'TGCA_C
Cuts at: 2066 2066
Size: 3296
ApoI r'AATT_y
Cuts at: 300 2649 2905 300
Size: 2349 256 691
Fragments arranged by size:
2349 691 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3296
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3296
AvaI C'yCGr_G
Cuts at: 316 316
Size: 3296
BamHI G'GATC_C
Cuts at: 321 321
Size: 3296
BanI G'GyrC_C
Cuts at: 312 726 974 1009 312
Size: 414 248 35 2599
Fragments arranged by size:
2599 414 248 35
BanII G_rGCy'C
Cuts at: 310 1340 310
Size: 1030 2266
BbeI G_GCGC'C
Cuts at: 730 978 730
Size: 248 3048
BciVI GTATCCnnnnn_n'
Cuts at: 1194 1955 3296 1194
Size: 761 1341 1194
Fragments arranged by size:
1341 1194 761
BclI T'GATC_A
Cuts at: 816 816
Size: 3296
BglI GCCn_nnn'nGGC
Cuts at: 224 224
Size: 3296
BglII A'GATC_T
Cuts at: 811 2848 811
Size: 2037 1259
BlpI GC'TnA_GC
Cuts at: 408 408
Size: 3296
Bme1580I G_kGCm'C
Cuts at: 243 921 1014 2070 243
Size: 678 93 1056 1469
Fragments arranged by size:
1469 1056 678 93
BmrI ACTGGGnnnn_n'
Cuts at: 508 589 924 3068 508
Size: 81 335 2144 736
Fragments arranged by size:
2144 736 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1663 1663
Size: 3296
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 472 1351 1843 2141 472
Size: 879 492 298 1627
Fragments arranged by size:
1627 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3000 3000
Size: 3296
BsaAI yAC'GTr
Cuts at: 649 649
Size: 3296
BsaBI GATnn'nnATC
Cuts at: 6 810 2847 6
Size: 804 2037 455
Fragments arranged by size:
2037 804 455
BsaHI Gr'CG_yC
Cuts at: 265 727 975 1677 3173 265
Size: 462 248 702 1496 388
Fragments arranged by size:
1496 702 462 388 248
BsaWI w'CCGG_w
Cuts at: 485 1006 1684 1958 2105 2516 485
Size: 521 678 274 147 411 1265
Fragments arranged by size:
1265 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 721 1479 2056 2552 721
Size: 758 577 496 1465
Fragments arranged by size:
1465 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 297 297
Size: 3296
BsiEI CG_ry'CG
Cuts at: 104 838 884 2092 104
Size: 734 46 1208 1308
Fragments arranged by size:
1308 1208 734 46
BsiHKAI G_wGCw'C
Cuts at: 310 1088 1278 2070 310
Size: 778 190 792 1536
Fragments arranged by size:
1536 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 243 310 921 1014 1088 1278 1340 2070 243
Size: 67 611 93 74 190 62 730 1469
Fragments arranged by size:
1469 730 611 190 93 74 67 62
BspEI T'CCGG_A
Cuts at: 2516 2516
Size: 3296
BspHI T'CATG_A
Cuts at: 2472 2472
Size: 3296
BspMI ACCTGCnnnn'nnnn_
Cuts at: 346 560 862 1243 346
Size: 214 302 381 2399
Fragments arranged by size:
2399 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1588 1642 3126 3291 50
Size: 1538 54 1484 165 55
Fragments arranged by size:
1538 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1208 1208
Size: 3296
BsrFI r'CCGG_y
Cuts at: 154 1294 1475 1684 154
Size: 1140 181 209 1766
Fragments arranged by size:
1766 1140 209 181
BssSI C'ACGA_G
Cuts at: 1567 1925 1567
Size: 358 2938
BstAPI GCAn_nnn'nTGC
Cuts at: 176 191 673 176
Size: 15 482 2799
Fragments arranged by size:
2799 482 15
BstBI TT'CG_AA
Cuts at: 1657 1657
Size: 3296
BstYI r'GATC_y
Cuts at: 1 321 788 811 1146 1392 2404 2490 2502
Size: 320 467 23 335 246 1012 86 12
Cuts at: 2502 2848 1
Size: 346 449
Fragments arranged by size:
1012 467 449 346 335 320 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3296
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1246 1427 1590 1246
Size: 181 163 2952
Fragments arranged by size:
2952 181 163
BtsI GCAGTG_nn'
Cuts at: 539 539
Size: 3296
DraI TTT'AAA
Cuts at: 295 295
Size: 3296
DrdI GACnn_nn'nnGTC
Cuts at: 1002 1860 1002
Size: 858 2438
EaeI y'GGCC_r
Cuts at: 881 1055 1446 1473 2818 881
Size: 174 391 27 1345 1359
Fragments arranged by size:
1359 1345 391 174 27
EagI C'GGCC_G
Cuts at: 881 881
Size: 3296
EarI CTCTTCn'nnn_
Cuts at: 193 1319 1529 193
Size: 1126 210 1960
Fragments arranged by size:
1960 1126 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1504 1814 1960 3106 1504
Size: 310 146 1146 1694
Fragments arranged by size:
1694 1146 310 146
EcoICRI GAG'CTC
Cuts at: 308 308
Size: 3296
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1121 1553 2300 2568 1121
Size: 432 747 268 1849
Fragments arranged by size:
1849 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 435 435
Size: 3296
EcoRI G'AATT_C
Cuts at: 300 300
Size: 3296
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1315 1315
Size: 3296
FspI TGC'GCA
Cuts at: 1077 1077
Size: 3296
HaeII r_GCGC'y
Cuts at: 730 978 2000 2979 730
Size: 248 1022 979 1047
Fragments arranged by size:
1047 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1138 1168 1170 1200 1138
Size: 30 2 30 3234
Fragments arranged by size:
3234 30 30 2
HincII GTy'rAC
Cuts at: 335 335
Size: 3296
KasI G'GCGC_C
Cuts at: 726 974 726
Size: 248 3048
KpnI G_GTAC'C
Cuts at: 316 316
Size: 3296
MluI A'CGCG_T
Cuts at: 2729 2729
Size: 3296
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1967 2151 1967
Size: 184 3112
MscI TGG'CCA
Cuts at: 1057 1057
Size: 3296
MslI CAynn'nnrTG
Cuts at: 1285 1412 1285
Size: 127 3169
MspA1I CmG'CkG
Cuts at: 281 407 721 1081 2094 2339 281
Size: 126 314 360 1013 245 1238
Fragments arranged by size:
1238 1013 360 314 245 126
NaeI GCC'GGC
Cuts at: 156 1477 156
Size: 1321 1975
NarI GG'CG_CC
Cuts at: 727 975 727
Size: 248 3048
NcoI C'CATG_G
Cuts at: 107 107
Size: 3296
NgoMIV G'CCGG_C
Cuts at: 154 1475 154
Size: 1321 1975
NspI r_CATG'y
Cuts at: 349 849 1756 349
Size: 500 907 1889
Fragments arranged by size:
1889 907 500
PasI CC'CwG_GG
Cuts at: 243 243
Size: 3296
PciI A'CATG_T
Cuts at: 1752 1752
Size: 3296
PmeI GTTT'AAAC
Cuts at: 295 295
Size: 3296
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2435 2467 2435
Size: 32 3264
PstI C_TGCA'G
Cuts at: 343 1028 343
Size: 685 2611
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3296
PvuII CAG'CTG
Cuts at: 281 721 1081 281
Size: 440 360 2496
Fragments arranged by size:
2496 440 360
RsrII CG'GwC_CG
Cuts at: 1491 1491
Size: 3296
SacI G_AGCT'C
Cuts at: 310 310
Size: 3296
SalI G'TCGA_C
Cuts at: 333 333
Size: 3296
SapI GCTCTTCn'nnn_
Cuts at: 193 1319 1529 193
Size: 1126 210 1960
Fragments arranged by size:
1960 1126 210
SbfI CC_TGCA'GG
Cuts at: 343 343
Size: 3296
ScaI AGT'ACT
Cuts at: 2813 2813
Size: 3296
SfcI C'TryA_G
Cuts at: 33 339 1024 2017 2208 33
Size: 306 685 993 191 1121
Fragments arranged by size:
1121 993 685 306 191
SfoI GGC'GCC
Cuts at: 728 976 728
Size: 248 3048
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3296
SmaI CCC'GGG
Cuts at: 318 318
Size: 3296
SmlI C'TyrA_G
Cuts at: 451 1366 1858 2120 451
Size: 915 492 262 1627
Fragments arranged by size:
1627 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3296
SphI G_CATG'C
Cuts at: 349 849 349
Size: 500 2796
StyI C'CwwG_G
Cuts at: 107 430 784 107
Size: 323 354 2619
Fragments arranged by size:
2619 354 323
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 879 1012 1471 1680 879
Size: 133 459 209 2495
Fragments arranged by size:
2495 459 209 133
TatI w'GTAC_w
Cuts at: 2811 2811
Size: 3296
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2813 2845 2813
Size: 32 3264
Tth111I GACn'n_nGTC
Cuts at: 1093 1093
Size: 3296
XbaI T'CTAG_A
Cuts at: 327 327
Size: 3296
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 791 791
Size: 3296
XmaI C'CCGG_G
Cuts at: 316 316
Size: 3296
ZraI GAC'GTC
Cuts at: 266 266
Size: 3296
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BmgBI
BmtI BpmI Bpu10I BsaXI BseRI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PflMI PfoI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI