Restriction map for
pF1KE0213
(Circular) (Six-base) MAPSORT of: pF1KE0213.seq Check: 9052 from: 1 to: 4073
pF1KE0213 4073 bp
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AccI GT'mk_AC
Cuts at: 846 1111 846
Size: 265 3808
Acc65I G'GTAC_C
Cuts at: 1089 1089
Size: 4073
AclI AA'CG_TT
Cuts at: 3913 3913
Size: 4073
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 485 527 562 1898 2330 3077 3345 485
Size: 42 35 1336 432 747 268 1213
Fragments arranged by size:
1336 1213 747 432 268 42 35
AflIII A'CryG_T
Cuts at: 1425 2529 3506 1425
Size: 1104 977 1992
Fragments arranged by size:
1992 1104 977
AgeI A'CCGG_T
Cuts at: 2461 2461
Size: 4073
AlwNI CAG_nnn'CTG
Cuts at: 1378 2945 1378
Size: 1567 2506
ApaLI G'TGCA_C
Cuts at: 2843 2843
Size: 4073
ApoI r'AATT_y
Cuts at: 334 978 1077 3426 3682 334
Size: 644 99 2349 256 725
Fragments arranged by size:
2349 725 644 256 99
AseI AT'TA_AT
Cuts at: 20 346 20
Size: 326 3747
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4073
AvaI C'yCGr_G
Cuts at: 1093 1093
Size: 4073
BamHI G'GATC_C
Cuts at: 1098 1098
Size: 4073
BanI G'GyrC_C
Cuts at: 1089 1503 1751 1786 1089
Size: 414 248 35 3376
Fragments arranged by size:
3376 414 248 35
BanII G_rGCy'C
Cuts at: 1087 2117 1087
Size: 1030 3043
BbeI G_GCGC'C
Cuts at: 1507 1755 1507
Size: 248 3825
BciVI GTATCCnnnnn_n'
Cuts at: 1971 2732 4073 1971
Size: 761 1341 1971
Fragments arranged by size:
1971 1341 761
BclI T'GATC_A
Cuts at: 903 1593 903
Size: 690 3383
BglII A'GATC_T
Cuts at: 1588 3625 1588
Size: 2037 2036
BlpI GC'TnA_GC
Cuts at: 251 1185 251
Size: 934 3139
Bme1580I G_kGCm'C
Cuts at: 205 1698 1791 2847 205
Size: 1493 93 1056 1431
Fragments arranged by size:
1493 1431 1056 93
BmgBI CAC'GTC
Cuts at: 528 528
Size: 4073
BmrI ACTGGGnnnn_n'
Cuts at: 1285 1366 1701 3845 1285
Size: 81 335 2144 1513
Fragments arranged by size:
2144 1513 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2440 2440
Size: 4073
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 674 674
Size: 4073
Bpu10I CC'TnA_GC
Cuts at: 635 635
Size: 4073
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 495 602 1249 2128 2620 2918 495
Size: 107 647 879 492 298 1650
Fragments arranged by size:
1650 879 647 492 298 107
BsaI GGTCTCn'nnnn_
Cuts at: 584 859 3777 584
Size: 275 2918 880
Fragments arranged by size:
2918 880 275
BsaAI yAC'GTr
Cuts at: 1426 1426
Size: 4073
BsaBI GATnn'nnATC
Cuts at: 6 1587 3624 6
Size: 1581 2037 455
Fragments arranged by size:
2037 1581 455
BsaHI Gr'CG_yC
Cuts at: 1504 1752 2454 3950 1504
Size: 248 702 1496 1627
Fragments arranged by size:
1627 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1262 1783 2461 2735 2882 3293 1262
Size: 521 678 274 147 411 2042
Fragments arranged by size:
2042 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 226 226
Size: 4073
BseYI C'CCAG_C
Cuts at: 892 1498 2256 2833 3329 892
Size: 606 758 577 496 1636
Fragments arranged by size:
1636 758 606 577 496
BsiEI CG_ry'CG
Cuts at: 104 1615 1661 2869 104
Size: 1511 46 1208 1308
Fragments arranged by size:
1511 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1087 1865 2055 2847 1087
Size: 778 190 792 2313
Fragments arranged by size:
2313 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 205 1087 1698 1791 1865 2055 2117 2847 205
Size: 882 611 93 74 190 62 730 1431
Fragments arranged by size:
1431 882 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3293 3293
Size: 4073
BspHI T'CATG_A
Cuts at: 3249 3249
Size: 4073
BspMI ACCTGCnnnn'nnnn_
Cuts at: 858 1123 1337 1639 2020 858
Size: 265 214 302 381 2911
Fragments arranged by size:
2911 381 302 265 214
BsrBI CCG'CTC
Cuts at: 50 159 2365 2419 3903 4068 50
Size: 109 2206 54 1484 165 55
Fragments arranged by size:
2206 1484 165 109 55 54
BsrDI GCAATG_nn'
Cuts at: 1985 1985
Size: 4073
BsrFI r'CCGG_y
Cuts at: 2071 2252 2461 2071
Size: 181 209 3683
Fragments arranged by size:
3683 209 181
BssSI C'ACGA_G
Cuts at: 2344 2702 2344
Size: 358 3715
BstAPI GCAn_nnn'nTGC
Cuts at: 1450 1450
Size: 4073
BstBI TT'CG_AA
Cuts at: 840 2434 840
Size: 1594 2479
BstYI r'GATC_y
Cuts at: 1 297 1098 1565 1588 1923 2169 3181 3267
Size: 296 801 467 23 335 246 1012 86
Cuts at: 3267 3279 3625 1
Size: 12 346 449
Fragments arranged by size:
1012 801 467 449 346 335 296 246
86 23 12
BstZ17I GTA'TAC
Cuts at: 847 847
Size: 4073
BtgI C'CryG_G
Cuts at: 107 107
Size: 4073
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2023 2204 2367 2023
Size: 181 163 3729
Fragments arranged by size:
3729 181 163
BtsI GCAGTG_nn'
Cuts at: 1316 1316
Size: 4073
DraI TTT'AAA
Cuts at: 1072 1072
Size: 4073
DraIII CAC_nnn'GTG
Cuts at: 712 712
Size: 4073
DrdI GACnn_nn'nnGTC
Cuts at: 1779 2637 1779
Size: 858 3215
EaeI y'GGCC_r
Cuts at: 1658 1832 2223 2250 3595 1658
Size: 174 391 27 1345 2136
Fragments arranged by size:
2136 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1658 1658
Size: 4073
EarI CTCTTCn'nnn_
Cuts at: 182 1046 2096 2306 182
Size: 864 1050 210 1949
Fragments arranged by size:
1949 1050 864 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2281 2591 2737 3883 2281
Size: 310 146 1146 2471
Fragments arranged by size:
2471 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1085 1085
Size: 4073
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 485 527 562 674 1898 2330 3077 3345 485
Size: 42 35 112 1224 432 747 268 1213
Fragments arranged by size:
1224 1213 747 432 268 112 42 35
EcoO109I rG'GnC_Cy
Cuts at: 1212 1212
Size: 4073
EcoRI G'AATT_C
Cuts at: 1077 1077
Size: 4073
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2092 2092
Size: 4073
FspI TGC'GCA
Cuts at: 1854 1854
Size: 4073
HaeII r_GCGC'y
Cuts at: 258 1507 1755 2777 3756 258
Size: 1249 248 1022 979 575
Fragments arranged by size:
1249 1022 979 575 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 301 333 888 920 1915 1945 1947 1977 301
Size: 32 555 32 995 30 2 30 2397
Fragments arranged by size:
2397 995 555 32 32 30 30 2
HincII GTy'rAC
Cuts at: 233 1112 233
Size: 879 3194
KasI G'GCGC_C
Cuts at: 1503 1751 1503
Size: 248 3825
KpnI G_GTAC'C
Cuts at: 1093 1093
Size: 4073
MluI A'CGCG_T
Cuts at: 3506 3506
Size: 4073
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2744 2928 2744
Size: 184 3889
MscI TGG'CCA
Cuts at: 1834 1834
Size: 4073
MslI CAynn'nnrTG
Cuts at: 2062 2189 2062
Size: 127 3946
MspA1I CmG'CkG
Cuts at: 558 1184 1498 1858 2871 3116 558
Size: 626 314 360 1013 245 1515
Fragments arranged by size:
1515 1013 626 360 314 245
NaeI GCC'GGC
Cuts at: 2254 2254
Size: 4073
NarI GG'CG_CC
Cuts at: 1504 1752 1504
Size: 248 3825
NcoI C'CATG_G
Cuts at: 107 107
Size: 4073
NgoMIV G'CCGG_C
Cuts at: 2252 2252
Size: 4073
NspI r_CATG'y
Cuts at: 403 1126 1626 2533 403
Size: 723 500 907 1943
Fragments arranged by size:
1943 907 723 500
PciI A'CATG_T
Cuts at: 2529 2529
Size: 4073
PfoI T'CCnGG_A
Cuts at: 931 931
Size: 4073
PmeI GTTT'AAAC
Cuts at: 1072 1072
Size: 4073
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3212 3244 3212
Size: 32 4041
PstI C_TGCA'G
Cuts at: 273 1120 1805 273
Size: 847 685 2541
Fragments arranged by size:
2541 847 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4073
PvuII CAG'CTG
Cuts at: 1498 1858 1498
Size: 360 3713
RsrII CG'GwC_CG
Cuts at: 2268 2268
Size: 4073
SacI G_AGCT'C
Cuts at: 1087 1087
Size: 4073
SalI G'TCGA_C
Cuts at: 1110 1110
Size: 4073
SapI GCTCTTCn'nnn_
Cuts at: 1046 2096 2306 1046
Size: 1050 210 2813
Fragments arranged by size:
2813 1050 210
SbfI CC_TGCA'GG
Cuts at: 1120 1120
Size: 4073
ScaI AGT'ACT
Cuts at: 434 3590 434
Size: 3156 917
SfcI C'TryA_G
Cuts at: 33 269 1116 1801 2794 2985 33
Size: 236 847 685 993 191 1121
Fragments arranged by size:
1121 993 847 685 236 191
SfoI GGC'GCC
Cuts at: 1505 1753 1505
Size: 248 3825
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4073
SmaI CCC'GGG
Cuts at: 1095 1095
Size: 4073
SmlI C'TyrA_G
Cuts at: 510 617 1228 2143 2635 2897 510
Size: 107 611 915 492 262 1686
Fragments arranged by size:
1686 915 611 492 262 107
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4073
SphI G_CATG'C
Cuts at: 1126 1626 1126
Size: 500 3573
StyI C'CwwG_G
Cuts at: 107 1207 1561 107
Size: 1100 354 2619
Fragments arranged by size:
2619 1100 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1656 1789 2248 2457 1656
Size: 133 459 209 3272
Fragments arranged by size:
3272 459 209 133
TatI w'GTAC_w
Cuts at: 432 743 3588 432
Size: 311 2845 917
Fragments arranged by size:
2845 917 311
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3590 3622 3590
Size: 32 4041
Tth111I GACn'n_nGTC
Cuts at: 1870 1870
Size: 4073
XbaI T'CTAG_A
Cuts at: 1104 1104
Size: 4073
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1568 1568
Size: 4073
XmaI C'CCGG_G
Cuts at: 1093 1093
Size: 4073
XmnI GAAnn'nnTTC
Cuts at: 176 176
Size: 4073
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BstZ17I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PfoI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BglI BmtI BsaXI BsgI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstXI Bsu36I ClaI CspCI EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI ZraI