(Circular) (Six-base) MAPSORT of: pF1KE0214.seq Check: 2085 from: 1 to: 4496 pF1KE0214 4496 bp With 182 enzymes: SgfI * April 24, 2009 13:11 .. AccI GT'mk_AC Cuts at: 367 1534 367 Size: 1167 3329 Acc65I G'GTAC_C Cuts at: 1252 1346 1512 1252 Size: 94 166 4236 Fragments arranged by size: 4236 166 94 AclI AA'CG_TT Cuts at: 4336 4336 Size: 4496 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1466 2321 2753 3500 3768 1466 Size: 855 432 747 268 2194 Fragments arranged by size: 2194 855 747 432 268 AflIII A'CryG_T Cuts at: 1848 2952 3929 1848 Size: 1104 977 2415 Fragments arranged by size: 2415 1104 977 AgeI A'CCGG_T Cuts at: 166 2884 166 Size: 2718 1778 AleI CACnn'nnGTG Cuts at: 1192 1192 Size: 4496 AlwNI CAG_nnn'CTG Cuts at: 1336 1801 3368 1336 Size: 465 1567 2464 Fragments arranged by size: 2464 1567 465 ApaLI G'TGCA_C Cuts at: 3266 3266 Size: 4496 ApoI r'AATT_y Cuts at: 409 1322 1500 3849 4105 409 Size: 913 178 2349 256 800 Fragments arranged by size: 2349 913 800 256 178 AseI AT'TA_AT Cuts at: 20 349 20 Size: 329 4167 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4496 AvaI C'yCGr_G Cuts at: 1516 1516 Size: 4496 AvrII C'CTAG_G Cuts at: 1044 1044 Size: 4496 BamHI G'GATC_C Cuts at: 1521 1521 Size: 4496 BanI G'GyrC_C Cuts at: 1173 1252 1346 1512 1926 2174 2209 1173 Size: 79 94 166 414 248 35 3460 Fragments arranged by size: 3460 414 248 166 94 79 35 BanII G_rGCy'C Cuts at: 1510 2540 1510 Size: 1030 3466 BbeI G_GCGC'C Cuts at: 1930 2178 1930 Size: 248 4248 BbsI GAAGACnn'nnnn_ Cuts at: 419 419 Size: 4496 BciVI GTATCCnnnnn_n' Cuts at: 2394 3155 4496 2394 Size: 761 1341 2394 Fragments arranged by size: 2394 1341 761 BclI T'GATC_A Cuts at: 393 576 2016 393 Size: 183 1440 2873 Fragments arranged by size: 2873 1440 183 BglII A'GATC_T Cuts at: 2011 4048 2011 Size: 2037 2459 BlpI GC'TnA_GC Cuts at: 1608 1608 Size: 4496 Bme1580I G_kGCm'C Cuts at: 2121 2214 3270 2121 Size: 93 1056 3347 Fragments arranged by size: 3347 1056 93 BmrI ACTGGGnnnn_n' Cuts at: 1708 1789 2124 4268 1708 Size: 81 335 2144 1936 Fragments arranged by size: 2144 1936 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2863 2863 Size: 4496 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1672 2551 3043 3341 1672 Size: 879 492 298 2827 Fragments arranged by size: 2827 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 4200 4200 Size: 4496 BsaAI yAC'GTr Cuts at: 1849 1849 Size: 4496 BsaBI GATnn'nnATC Cuts at: 6 2010 4047 6 Size: 2004 2037 455 Fragments arranged by size: 2037 2004 455 BsaHI Gr'CG_yC Cuts at: 1927 2175 2877 4373 1927 Size: 248 702 1496 2050 Fragments arranged by size: 2050 1496 702 248 BsaWI w'CCGG_w Cuts at: 166 1685 2206 2884 3158 3305 3716 166 Size: 1519 521 678 274 147 411 946 Fragments arranged by size: 1519 946 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 755 785 755 Size: 30 4466 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1307 1307 Size: 4496 BseYI C'CCAG_C Cuts at: 1921 2679 3256 3752 1921 Size: 758 577 496 2665 Fragments arranged by size: 2665 758 577 496 BsiEI CG_ry'CG Cuts at: 104 2038 2084 3292 104 Size: 1934 46 1208 1308 Fragments arranged by size: 1934 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 441 1510 2288 2478 3270 441 Size: 1069 778 190 792 1667 Fragments arranged by size: 1667 1069 792 778 190 BsmI GAATG_Cn' Cuts at: 286 864 286 Size: 578 3918 BsmBI CGTCTCn'nnnn_ Cuts at: 867 867 Size: 4496 Bsp1286I G_dGCh'C Cuts at: 441 1510 2121 2214 2288 2478 2540 3270 441 Size: 1069 611 93 74 190 62 730 1667 Fragments arranged by size: 1667 1069 730 611 190 93 74 62 BspEI T'CCGG_A Cuts at: 3716 3716 Size: 4496 BspHI T'CATG_A Cuts at: 3672 3672 Size: 4496 BspMI ACCTGCnnnn'nnnn_ Cuts at: 138 1546 1760 2062 2443 138 Size: 1408 214 302 381 2191 Fragments arranged by size: 2191 1408 381 302 214 BsrBI CCG'CTC Cuts at: 50 2788 2842 4326 4491 50 Size: 2738 54 1484 165 55 Fragments arranged by size: 2738 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 935 2408 935 Size: 1473 3023 BsrFI r'CCGG_y Cuts at: 166 2494 2675 2884 166 Size: 2328 181 209 1778 Fragments arranged by size: 2328 1778 209 181 BssSI C'ACGA_G Cuts at: 2767 3125 2767 Size: 358 4138 BstAPI GCAn_nnn'nTGC Cuts at: 1873 1873 Size: 4496 BstBI TT'CG_AA Cuts at: 609 2857 609 Size: 2248 2248 BstXI CCAn_nnnn'nTGG Cuts at: 980 980 Size: 4496 BstYI r'GATC_y Cuts at: 1 765 1521 1988 2011 2346 2592 3604 3690 Size: 764 756 467 23 335 246 1012 86 Cuts at: 3690 3702 4048 1 Size: 12 346 449 Fragments arranged by size: 1012 764 756 467 449 346 335 246 86 23 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2446 2627 2790 2446 Size: 181 163 4152 Fragments arranged by size: 4152 181 163 BtsI GCAGTG_nn' Cuts at: 1298 1739 1298 Size: 441 4055 DraI TTT'AAA Cuts at: 1495 1495 Size: 4496 DrdI GACnn_nn'nnGTC Cuts at: 2202 3060 2202 Size: 858 3638 EaeI y'GGCC_r Cuts at: 2081 2255 2646 2673 4018 2081 Size: 174 391 27 1345 2559 Fragments arranged by size: 2559 1345 391 174 27 EagI C'GGCC_G Cuts at: 2081 2081 Size: 4496 EarI CTCTTCn'nnn_ Cuts at: 2519 2729 2519 Size: 210 4286 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2704 3014 3160 4306 2704 Size: 310 146 1146 2894 Fragments arranged by size: 2894 1146 310 146 EcoICRI GAG'CTC Cuts at: 1508 1508 Size: 4496 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1466 2321 2753 3500 3768 1466 Size: 855 432 747 268 2194 Fragments arranged by size: 2194 855 747 432 268 EcoO109I rG'GnC_Cy Cuts at: 1635 1635 Size: 4496 EcoRI G'AATT_C Cuts at: 1500 1500 Size: 4496 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2515 2515 Size: 4496 FspI TGC'GCA Cuts at: 2277 2277 Size: 4496 HaeII r_GCGC'y Cuts at: 1930 2178 3200 4179 1930 Size: 248 1022 979 2247 Fragments arranged by size: 2247 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 769 801 869 901 2338 2368 2370 2400 769 Size: 32 68 32 1437 30 2 30 2865 Fragments arranged by size: 2865 1437 68 32 32 30 30 2 HincII GTy'rAC Cuts at: 1535 1535 Size: 4496 KasI G'GCGC_C Cuts at: 1926 2174 1926 Size: 248 4248 KpnI G_GTAC'C Cuts at: 1256 1350 1516 1256 Size: 94 166 4236 Fragments arranged by size: 4236 166 94 MluI A'CGCG_T Cuts at: 3929 3929 Size: 4496 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 3167 3351 3167 Size: 184 4312 MscI TGG'CCA Cuts at: 2257 2257 Size: 4496 MslI CAynn'nnrTG Cuts at: 1192 2485 2612 1192 Size: 1293 127 3076 Fragments arranged by size: 3076 1293 127 MspA1I CmG'CkG Cuts at: 1427 1607 1921 2281 3294 3539 1427 Size: 180 314 360 1013 245 2384 Fragments arranged by size: 2384 1013 360 314 245 180 NaeI GCC'GGC Cuts at: 2677 2677 Size: 4496 NarI GG'CG_CC Cuts at: 1927 2175 1927 Size: 248 4248 NdeI CA'TA_TG Cuts at: 1085 1085 Size: 4496 NgoMIV G'CCGG_C Cuts at: 2675 2675 Size: 4496 NspI r_CATG'y Cuts at: 849 1549 2049 2956 849 Size: 700 500 907 2389 Fragments arranged by size: 2389 907 700 500 PciI A'CATG_T Cuts at: 2952 2952 Size: 4496 PflMI CCAn_nnn'nTGG Cuts at: 455 548 455 Size: 93 4403 PfoI T'CCnGG_A Cuts at: 290 290 Size: 4496 PmeI GTTT'AAAC Cuts at: 1495 1495 Size: 4496 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3635 3667 3635 Size: 32 4464 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 1439 1471 1439 Size: 32 4464 PstI C_TGCA'G Cuts at: 332 1305 1543 2228 332 Size: 973 238 685 2600 Fragments arranged by size: 2600 973 685 238 PvuI CG_AT'CG Cuts at: 104 104 Size: 4496 PvuII CAG'CTG Cuts at: 1427 1921 2281 1427 Size: 494 360 3642 Fragments arranged by size: 3642 494 360 RsrII CG'GwC_CG Cuts at: 2691 2691 Size: 4496 SacI G_AGCT'C Cuts at: 1510 1510 Size: 4496 SalI G'TCGA_C Cuts at: 1533 1533 Size: 4496 SapI GCTCTTCn'nnn_ Cuts at: 2519 2729 2519 Size: 210 4286 SbfI CC_TGCA'GG Cuts at: 1543 1543 Size: 4496 ScaI AGT'ACT Cuts at: 4013 4013 Size: 4496 SfcI C'TryA_G Cuts at: 33 328 1301 1539 2224 3217 3408 33 Size: 295 973 238 685 993 191 1121 Fragments arranged by size: 1121 993 973 685 295 238 191 SfoI GGC'GCC Cuts at: 1928 2176 1928 Size: 248 4248 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4496 SmaI CCC'GGG Cuts at: 1518 1518 Size: 4496 SmlI C'TyrA_G Cuts at: 1651 2566 3058 3320 1651 Size: 915 492 262 2827 Fragments arranged by size: 2827 915 492 262 SpeI A'CTAG_T Cuts at: 67 1036 67 Size: 969 3527 SphI G_CATG'C Cuts at: 1549 2049 1549 Size: 500 3996 SspI AAT'ATT Cuts at: 246 246 Size: 4496 StyI C'CwwG_G Cuts at: 1044 1630 1984 1044 Size: 586 354 3556 Fragments arranged by size: 3556 586 354 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 2079 2212 2671 2880 2079 Size: 133 459 209 3695 Fragments arranged by size: 3695 459 209 133 TatI w'GTAC_w Cuts at: 1144 4011 1144 Size: 2867 1629 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 755 787 4013 4045 755 Size: 32 3226 32 1206 Fragments arranged by size: 3226 1206 32 32 Tth111I GACn'n_nGTC Cuts at: 2293 2293 Size: 4496 XbaI T'CTAG_A Cuts at: 1527 1527 Size: 4496 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1991 1991 Size: 4496 XmaI C'CCGG_G Cuts at: 1516 1516 Size: 4496 XmnI GAAnn'nnTTC Cuts at: 1079 1079 Size: 4496 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NdeI NgoMIV NspI PciI PflMI PfoI PmeI PpiI PsrI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AlfI AloI ApaI AscI BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI BpmI Bpu10I BsgI BsiWI BsrGI BssHII BstEII BstZ17I Bsu36I BtgI ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NcoI NheI NotI NruI NsiI PacI PasI PmlI PpuMI PshAI PsiI PspOMI PspXI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI StuI SwaI XhoI ZraI