Restriction map for
pF1KE0217
(Circular) (Six-base) MAPSORT of: pF1KE0217.seq Check: 731 from: 1 to: 3629
pF1KE0217 3629 bp
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AccI GT'mk_AC
Cuts at: 287 667 287
Size: 380 3249
Acc65I G'GTAC_C
Cuts at: 645 645
Size: 3629
AclI AA'CG_TT
Cuts at: 565 3469 565
Size: 2904 725
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1454 1886 2633 2901 1454
Size: 432 747 268 2182
Fragments arranged by size:
2182 747 432 268
AflIII A'CryG_T
Cuts at: 197 981 2085 3062 197
Size: 784 1104 977 764
Fragments arranged by size:
1104 977 784 764
AgeI A'CCGG_T
Cuts at: 2017 2017
Size: 3629
AlwNI CAG_nnn'CTG
Cuts at: 934 2501 934
Size: 1567 2062
ApaLI G'TGCA_C
Cuts at: 2399 2399
Size: 3629
ApoI r'AATT_y
Cuts at: 402 488 633 2982 3238 402
Size: 86 145 2349 256 793
Fragments arranged by size:
2349 793 256 145 86
AseI AT'TA_AT
Cuts at: 20 20
Size: 3629
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3629
AvaI C'yCGr_G
Cuts at: 649 649
Size: 3629
BamHI G'GATC_C
Cuts at: 654 654
Size: 3629
BanI G'GyrC_C
Cuts at: 645 1059 1307 1342 645
Size: 414 248 35 2932
Fragments arranged by size:
2932 414 248 35
BanII G_rGCy'C
Cuts at: 181 643 1673 181
Size: 462 1030 2137
Fragments arranged by size:
2137 1030 462
BbeI G_GCGC'C
Cuts at: 1063 1311 1063
Size: 248 3381
BbsI GAAGACnn'nnnn_
Cuts at: 344 344
Size: 3629
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 115 149 115
Size: 34 3595
BciVI GTATCCnnnnn_n'
Cuts at: 1527 2288 3629 1527
Size: 761 1341 1527
Fragments arranged by size:
1527 1341 761
BclI T'GATC_A
Cuts at: 1149 1149
Size: 3629
BglI GCCn_nnn'nGGC
Cuts at: 355 355
Size: 3629
BglII A'GATC_T
Cuts at: 1144 3181 1144
Size: 2037 1592
BlpI GC'TnA_GC
Cuts at: 741 741
Size: 3629
Bme1580I G_kGCm'C
Cuts at: 236 1254 1347 2403 236
Size: 1018 93 1056 1462
Fragments arranged by size:
1462 1056 1018 93
BmgBI CAC'GTC
Cuts at: 198 198
Size: 3629
BmrI ACTGGGnnnn_n'
Cuts at: 841 922 1257 3401 841
Size: 81 335 2144 1069
Fragments arranged by size:
2144 1069 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1996 1996
Size: 3629
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 167 589 167
Size: 422 3207
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 805 1684 2176 2474 805
Size: 879 492 298 1960
Fragments arranged by size:
1960 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 246 593 3333 246
Size: 347 2740 542
Fragments arranged by size:
2740 542 347
BsaAI yAC'GTr
Cuts at: 982 982
Size: 3629
BsaBI GATnn'nnATC
Cuts at: 6 280 1143 3180 6
Size: 274 863 2037 455
Fragments arranged by size:
2037 863 455 274
BsaHI Gr'CG_yC
Cuts at: 1060 1308 2010 3506 1060
Size: 248 702 1496 1183
Fragments arranged by size:
1496 1183 702 248
BsaWI w'CCGG_w
Cuts at: 818 1339 2017 2291 2438 2849 818
Size: 521 678 274 147 411 1598
Fragments arranged by size:
1598 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1054 1812 2389 2885 1054
Size: 758 577 496 1798
Fragments arranged by size:
1798 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1171 1217 2425 104
Size: 1067 46 1208 1308
Fragments arranged by size:
1308 1208 1067 46
BsiHKAI G_wGCw'C
Cuts at: 181 503 643 1421 1611 2403 181
Size: 322 140 778 190 792 1407
Fragments arranged by size:
1407 792 778 322 190 140
BsmI GAATG_Cn'
Cuts at: 557 557
Size: 3629
Bsp1286I G_dGCh'C
Cuts at: 181 236 503 643 1254 1347 1421 1611 1673
Size: 55 267 140 611 93 74 190 62
Cuts at: 1673 2403 181
Size: 730 1407
Fragments arranged by size:
1407 730 611 267 190 140 93 74
62 55
BspEI T'CCGG_A
Cuts at: 2849 2849
Size: 3629
BspHI T'CATG_A
Cuts at: 2805 2805
Size: 3629
BspMI ACCTGCnnnn'nnnn_
Cuts at: 679 893 1195 1576 679
Size: 214 302 381 2732
Fragments arranged by size:
2732 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1921 1975 3459 3624 50
Size: 1871 54 1484 165 55
Fragments arranged by size:
1871 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1541 1541
Size: 3629
BsrFI r'CCGG_y
Cuts at: 1627 1808 2017 1627
Size: 181 209 3239
Fragments arranged by size:
3239 209 181
BssSI C'ACGA_G
Cuts at: 1900 2258 1900
Size: 358 3271
BstAPI GCAn_nnn'nTGC
Cuts at: 1006 1006
Size: 3629
BstBI TT'CG_AA
Cuts at: 1990 1990
Size: 3629
BstYI r'GATC_y
Cuts at: 1 654 1121 1144 1479 1725 2737 2823 2835
Size: 653 467 23 335 246 1012 86 12
Cuts at: 2835 3181 1
Size: 346 449
Fragments arranged by size:
1012 653 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 137 107
Size: 30 3599
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 146 538 1579 1760 1923 146
Size: 392 1041 181 163 1852
Fragments arranged by size:
1852 1041 392 181 163
BtsI GCAGTG_nn'
Cuts at: 872 872
Size: 3629
DraI TTT'AAA
Cuts at: 164 628 164
Size: 464 3165
DrdI GACnn_nn'nnGTC
Cuts at: 1335 2193 1335
Size: 858 2771
EaeI y'GGCC_r
Cuts at: 122 156 1214 1388 1779 1806 3151 122
Size: 34 1058 174 391 27 1345 600
Fragments arranged by size:
1345 1058 600 391 174 34 27
EagI C'GGCC_G
Cuts at: 1214 1214
Size: 3629
EarI CTCTTCn'nnn_
Cuts at: 1652 1862 1652
Size: 210 3419
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1837 2147 2293 3439 1837
Size: 310 146 1146 2027
Fragments arranged by size:
2027 1146 310 146
EcoICRI GAG'CTC
Cuts at: 179 641 179
Size: 462 3167
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 167 589 1454 1886 2633 2901 167
Size: 422 865 432 747 268 895
Fragments arranged by size:
895 865 747 432 422 268
EcoO109I rG'GnC_Cy
Cuts at: 768 768
Size: 3629
EcoRI G'AATT_C
Cuts at: 633 633
Size: 3629
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1648 1648
Size: 3629
FspI TGC'GCA
Cuts at: 1410 1410
Size: 3629
HaeII r_GCGC'y
Cuts at: 1063 1311 2333 3312 1063
Size: 248 1022 979 1380
Fragments arranged by size:
1380 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1471 1501 1503 1533 1471
Size: 30 2 30 3567
Fragments arranged by size:
3567 30 30 2
HincII GTy'rAC
Cuts at: 668 668
Size: 3629
HindIII A'AGCT_T
Cuts at: 616 616
Size: 3629
KasI G'GCGC_C
Cuts at: 1059 1307 1059
Size: 248 3381
KpnI G_GTAC'C
Cuts at: 649 649
Size: 3629
MfeI C'AATT_G
Cuts at: 575 575
Size: 3629
MluI A'CGCG_T
Cuts at: 3062 3062
Size: 3629
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2300 2484 2300
Size: 184 3445
MscI TGG'CCA
Cuts at: 158 1390 158
Size: 1232 2397
MslI CAynn'nnrTG
Cuts at: 1618 1745 1618
Size: 127 3502
MspA1I CmG'CkG
Cuts at: 740 1054 1414 2427 2672 740
Size: 314 360 1013 245 1697
Fragments arranged by size:
1697 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1810 1810
Size: 3629
NarI GG'CG_CC
Cuts at: 1060 1308 1060
Size: 248 3381
NcoI C'CATG_G
Cuts at: 107 107
Size: 3629
NgoMIV G'CCGG_C
Cuts at: 1808 1808
Size: 3629
NspI r_CATG'y
Cuts at: 682 1182 2089 682
Size: 500 907 2222
Fragments arranged by size:
2222 907 500
PciI A'CATG_T
Cuts at: 2085 2085
Size: 3629
PflMI CCAn_nnn'nTGG
Cuts at: 229 229
Size: 3629
PmeI GTTT'AAAC
Cuts at: 628 628
Size: 3629
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2768 2800 2768
Size: 32 3597
PstI C_TGCA'G
Cuts at: 676 1361 676
Size: 685 2944
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3629
PvuII CAG'CTG
Cuts at: 1054 1414 1054
Size: 360 3269
RsrII CG'GwC_CG
Cuts at: 1824 1824
Size: 3629
SacI G_AGCT'C
Cuts at: 181 643 181
Size: 462 3167
SalI G'TCGA_C
Cuts at: 666 666
Size: 3629
SapI GCTCTTCn'nnn_
Cuts at: 1652 1862 1652
Size: 210 3419
SbfI CC_TGCA'GG
Cuts at: 676 676
Size: 3629
ScaI AGT'ACT
Cuts at: 3146 3146
Size: 3629
SfcI C'TryA_G
Cuts at: 33 672 1357 2350 2541 33
Size: 639 685 993 191 1121
Fragments arranged by size:
1121 993 685 639 191
SfoI GGC'GCC
Cuts at: 1061 1309 1061
Size: 248 3381
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3629
SmaI CCC'GGG
Cuts at: 651 651
Size: 3629
SmlI C'TyrA_G
Cuts at: 784 1699 2191 2453 784
Size: 915 492 262 1960
Fragments arranged by size:
1960 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3629
SphI G_CATG'C
Cuts at: 682 1182 682
Size: 500 3129
StyI C'CwwG_G
Cuts at: 107 763 1117 107
Size: 656 354 2619
Fragments arranged by size:
2619 656 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 518 1212 1345 1804 2013 518
Size: 694 133 459 209 2134
Fragments arranged by size:
2134 694 459 209 133
TatI w'GTAC_w
Cuts at: 200 3144 200
Size: 2944 685
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3146 3178 3146
Size: 32 3597
Tth111I GACn'n_nGTC
Cuts at: 1426 1426
Size: 3629
XbaI T'CTAG_A
Cuts at: 660 660
Size: 3629
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1124 1124
Size: 3629
XmaI C'CCGG_G
Cuts at: 649 649
Size: 3629
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PflMI PmeI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BfrBI BmtI Bpu10I
BsaXI BseRI BsgI BsiWI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HpaI NdeI NheI NotI NruI NsiI
PacI PasI PfoI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI