Restriction map for
pF1KE0221
(Circular) (Six-base) MAPSORT of: pF1KE0221.seq Check: 1959 from: 1 to: 3401
pF1KE0221 3401 bp
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AccI GT'mk_AC
Cuts at: 439 439
Size: 3401
Acc65I G'GTAC_C
Cuts at: 189 417 189
Size: 228 3173
AclI AA'CG_TT
Cuts at: 3241 3241
Size: 3401
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 325 1226 1658 2405 2673 325
Size: 901 432 747 268 1053
Fragments arranged by size:
1053 901 747 432 268
AflIII A'CryG_T
Cuts at: 753 1857 2834 753
Size: 1104 977 1320
Fragments arranged by size:
1320 1104 977
AgeI A'CCGG_T
Cuts at: 1789 1789
Size: 3401
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 199 199
Size: 3401
AlwNI CAG_nnn'CTG
Cuts at: 706 2273 706
Size: 1567 1834
ApaLI G'TGCA_C
Cuts at: 2171 2171
Size: 3401
ApoI r'AATT_y
Cuts at: 405 2754 3010 405
Size: 2349 256 796
Fragments arranged by size:
2349 796 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3401
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3401
AvaI C'yCGr_G
Cuts at: 421 421
Size: 3401
BamHI G'GATC_C
Cuts at: 426 426
Size: 3401
BanI G'GyrC_C
Cuts at: 189 417 831 1079 1114 189
Size: 228 414 248 35 2476
Fragments arranged by size:
2476 414 248 228 35
BanII G_rGCy'C
Cuts at: 415 1445 415
Size: 1030 2371
BbeI G_GCGC'C
Cuts at: 835 1083 835
Size: 248 3153
BbvCI CC'TCA_GC
Cuts at: 370 370
Size: 3401
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3367
BciVI GTATCCnnnnn_n'
Cuts at: 1299 2060 3401 1299
Size: 761 1341 1299
Fragments arranged by size:
1341 1299 761
BclI T'GATC_A
Cuts at: 921 921
Size: 3401
BglII A'GATC_T
Cuts at: 916 2953 916
Size: 2037 1364
BlpI GC'TnA_GC
Cuts at: 513 513
Size: 3401
Bme1580I G_kGCm'C
Cuts at: 1026 1119 2175 1026
Size: 93 1056 2252
Fragments arranged by size:
2252 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 613 694 1029 3173 613
Size: 81 335 2144 841
Fragments arranged by size:
2144 841 335 81
BmtI G_CTAG'C
Cuts at: 148 148
Size: 3401
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1768 1768
Size: 3401
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 183 183
Size: 3401
Bpu10I CC'TnA_GC
Cuts at: 370 370
Size: 3401
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 577 1456 1948 2246 577
Size: 879 492 298 1732
Fragments arranged by size:
1732 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3105 3105
Size: 3401
BsaAI yAC'GTr
Cuts at: 754 754
Size: 3401
BsaBI GATnn'nnATC
Cuts at: 6 915 2952 6
Size: 909 2037 455
Fragments arranged by size:
2037 909 455
BsaHI Gr'CG_yC
Cuts at: 832 1080 1782 3278 832
Size: 248 702 1496 955
Fragments arranged by size:
1496 955 702 248
BsaWI w'CCGG_w
Cuts at: 590 1111 1789 2063 2210 2621 590
Size: 521 678 274 147 411 1370
Fragments arranged by size:
1370 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 148 826 1584 2161 2657 148
Size: 678 758 577 496 892
Fragments arranged by size:
892 758 678 577 496
BsiEI CG_ry'CG
Cuts at: 104 943 989 2197 104
Size: 839 46 1208 1308
Fragments arranged by size:
1308 1208 839 46
BsiHKAI G_wGCw'C
Cuts at: 415 1193 1383 2175 415
Size: 778 190 792 1641
Fragments arranged by size:
1641 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 415 1026 1119 1193 1383 1445 2175 415
Size: 611 93 74 190 62 730 1641
Fragments arranged by size:
1641 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2621 2621
Size: 3401
BspHI T'CATG_A
Cuts at: 2577 2577
Size: 3401
BspMI ACCTGCnnnn'nnnn_
Cuts at: 177 451 665 967 1348 177
Size: 274 214 302 381 2230
Fragments arranged by size:
2230 381 302 274 214
BsrBI CCG'CTC
Cuts at: 50 1693 1747 3231 3396 50
Size: 1643 54 1484 165 55
Fragments arranged by size:
1643 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1313 1313
Size: 3401
BsrFI r'CCGG_y
Cuts at: 1399 1580 1789 1399
Size: 181 209 3011
Fragments arranged by size:
3011 209 181
BssSI C'ACGA_G
Cuts at: 1672 2030 1672
Size: 358 3043
BstAPI GCAn_nnn'nTGC
Cuts at: 778 778
Size: 3401
BstBI TT'CG_AA
Cuts at: 1762 1762
Size: 3401
BstXI CCAn_nnnn'nTGG
Cuts at: 129 129
Size: 3401
BstYI r'GATC_y
Cuts at: 1 426 893 916 1251 1497 2509 2595 2607
Size: 425 467 23 335 246 1012 86 12
Cuts at: 2607 2953 1
Size: 346 449
Fragments arranged by size:
1012 467 449 425 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 238 238
Size: 3401
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1351 1532 1695 1351
Size: 181 163 3057
Fragments arranged by size:
3057 181 163
BtsI GCAGTG_nn'
Cuts at: 375 644 375
Size: 269 3132
DraI TTT'AAA
Cuts at: 400 400
Size: 3401
DrdI GACnn_nn'nnGTC
Cuts at: 1107 1965 1107
Size: 858 2543
EaeI y'GGCC_r
Cuts at: 157 986 1160 1551 1578 2923 157
Size: 829 174 391 27 1345 635
Fragments arranged by size:
1345 829 635 391 174 27
EagI C'GGCC_G
Cuts at: 986 986
Size: 3401
EarI CTCTTCn'nnn_
Cuts at: 301 1424 1634 301
Size: 1123 210 2068
Fragments arranged by size:
2068 1123 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1609 1919 2065 3211 1609
Size: 310 146 1146 1799
Fragments arranged by size:
1799 1146 310 146
EcoICRI GAG'CTC
Cuts at: 413 413
Size: 3401
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 183 325 1226 1658 2405 2673 183
Size: 142 901 432 747 268 911
Fragments arranged by size:
911 901 747 432 268 142
EcoO109I rG'GnC_Cy
Cuts at: 540 540
Size: 3401
EcoRI G'AATT_C
Cuts at: 405 405
Size: 3401
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1420 1420
Size: 3401
FspI TGC'GCA
Cuts at: 1182 1182
Size: 3401
HaeII r_GCGC'y
Cuts at: 835 1083 2105 3084 835
Size: 248 1022 979 1152
Fragments arranged by size:
1152 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1243 1273 1275 1305 1243
Size: 30 2 30 3339
Fragments arranged by size:
3339 30 30 2
HincII GTy'rAC
Cuts at: 440 440
Size: 3401
KasI G'GCGC_C
Cuts at: 831 1079 831
Size: 248 3153
KpnI G_GTAC'C
Cuts at: 193 421 193
Size: 228 3173
MluI A'CGCG_T
Cuts at: 2834 2834
Size: 3401
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 321 2072 2256 321
Size: 1751 184 1466
Fragments arranged by size:
1751 1466 184
MscI TGG'CCA
Cuts at: 1162 1162
Size: 3401
MslI CAynn'nnrTG
Cuts at: 1390 1517 1390
Size: 127 3274
MspA1I CmG'CkG
Cuts at: 512 826 1186 2199 2444 512
Size: 314 360 1013 245 1469
Fragments arranged by size:
1469 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1582 1582
Size: 3401
NarI GG'CG_CC
Cuts at: 832 1080 832
Size: 248 3153
NgoMIV G'CCGG_C
Cuts at: 1580 1580
Size: 3401
NheI G'CTAG_C
Cuts at: 144 144
Size: 3401
NspI r_CATG'y
Cuts at: 186 454 954 1861 186
Size: 268 500 907 1726
Fragments arranged by size:
1726 907 500 268
PciI A'CATG_T
Cuts at: 1857 1857
Size: 3401
PmeI GTTT'AAAC
Cuts at: 400 400
Size: 3401
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2540 2572 2540
Size: 32 3369
PstI C_TGCA'G
Cuts at: 448 1133 448
Size: 685 2716
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3401
PvuII CAG'CTG
Cuts at: 826 1186 826
Size: 360 3041
RsrII CG'GwC_CG
Cuts at: 1596 1596
Size: 3401
SacI G_AGCT'C
Cuts at: 415 415
Size: 3401
SalI G'TCGA_C
Cuts at: 438 438
Size: 3401
SapI GCTCTTCn'nnn_
Cuts at: 1424 1634 1424
Size: 210 3191
SbfI CC_TGCA'GG
Cuts at: 448 448
Size: 3401
ScaI AGT'ACT
Cuts at: 2918 2918
Size: 3401
SfcI C'TryA_G
Cuts at: 33 444 1129 2122 2313 33
Size: 411 685 993 191 1121
Fragments arranged by size:
1121 993 685 411 191
SfoI GGC'GCC
Cuts at: 833 1081 833
Size: 248 3153
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3401
SmaI CCC'GGG
Cuts at: 423 423
Size: 3401
SmlI C'TyrA_G
Cuts at: 556 1471 1963 2225 556
Size: 915 492 262 1732
Fragments arranged by size:
1732 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3401
SphI G_CATG'C
Cuts at: 186 454 954 186
Size: 268 500 2633
Fragments arranged by size:
2633 500 268
SspI AAT'ATT
Cuts at: 296 296
Size: 3401
StuI AGG'CCT
Cuts at: 322 322
Size: 3401
StyI C'CwwG_G
Cuts at: 329 535 889 329
Size: 206 354 2841
Fragments arranged by size:
2841 354 206
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 984 1117 1576 1785 984
Size: 133 459 209 2600
Fragments arranged by size:
2600 459 209 133
TatI w'GTAC_w
Cuts at: 2916 2916
Size: 3401
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2918 2950 2918
Size: 32 3369
Tth111I GACn'n_nGTC
Cuts at: 1198 1198
Size: 3401
XbaI T'CTAG_A
Cuts at: 432 432
Size: 3401
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 896 896
Size: 3401
XmaI C'CCGG_G
Cuts at: 421 421
Size: 3401
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbvCI BcgI BciVI BclI BglII BlpI Bme1580I
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NheI NspI
PciI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbsI BfrBI BglI BmgBI BsaXI
BseRI BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstZ17I BtgI ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NcoI NdeI NotI NruI
NsiI PacI PasI PflMI PfoI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SwaI XhoI XmnI ZraI