Restriction map for
pF1KE0226
(Circular) (Six-base) MAPSORT of: pF1KE0226.seq Check: 1935 from: 1 to: 3497
pF1KE0226 3497 bp
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AccI GT'mk_AC
Cuts at: 535 535
Size: 3497
Acc65I G'GTAC_C
Cuts at: 513 513
Size: 3497
AclI AA'CG_TT
Cuts at: 3337 3337
Size: 3497
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 163 1322 1754 2501 2769 163
Size: 1159 432 747 268 891
Fragments arranged by size:
1159 891 747 432 268
AflIII A'CryG_T
Cuts at: 849 1953 2930 849
Size: 1104 977 1416
Fragments arranged by size:
1416 1104 977
AgeI A'CCGG_T
Cuts at: 1885 1885
Size: 3497
AlwNI CAG_nnn'CTG
Cuts at: 802 2369 802
Size: 1567 1930
ApaLI G'TGCA_C
Cuts at: 199 2267 199
Size: 2068 1429
ApoI r'AATT_y
Cuts at: 501 2850 3106 501
Size: 2349 256 892
Fragments arranged by size:
2349 892 256
AscI GG'CGCG_CC
Cuts at: 248 248
Size: 3497
AseI AT'TA_AT
Cuts at: 20 20
Size: 3497
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3497
AvaI C'yCGr_G
Cuts at: 167 517 167
Size: 350 3147
BamHI G'GATC_C
Cuts at: 522 522
Size: 3497
BanI G'GyrC_C
Cuts at: 513 927 1175 1210 513
Size: 414 248 35 2800
Fragments arranged by size:
2800 414 248 35
BanII G_rGCy'C
Cuts at: 389 511 1541 389
Size: 122 1030 2345
Fragments arranged by size:
2345 1030 122
BbeI G_GCGC'C
Cuts at: 931 1179 931
Size: 248 3249
BbsI GAAGACnn'nnnn_
Cuts at: 340 472 340
Size: 132 3365
BciVI GTATCCnnnnn_n'
Cuts at: 1395 2156 3497 1395
Size: 761 1341 1395
Fragments arranged by size:
1395 1341 761
BclI T'GATC_A
Cuts at: 1017 1017
Size: 3497
BglII A'GATC_T
Cuts at: 1012 3049 1012
Size: 2037 1460
BlpI GC'TnA_GC
Cuts at: 609 609
Size: 3497
Bme1580I G_kGCm'C
Cuts at: 203 1122 1215 2271 203
Size: 919 93 1056 1429
Fragments arranged by size:
1429 1056 919 93
BmgBI CAC'GTC
Cuts at: 118 118
Size: 3497
BmrI ACTGGGnnnn_n'
Cuts at: 709 790 1125 3269 709
Size: 81 335 2144 937
Fragments arranged by size:
2144 937 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1864 1864
Size: 3497
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 295 319 295
Size: 24 3473
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 673 1552 2044 2342 673
Size: 879 492 298 1828
Fragments arranged by size:
1828 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3201 3201
Size: 3497
BsaAI yAC'GTr
Cuts at: 850 850
Size: 3497
BsaBI GATnn'nnATC
Cuts at: 6 1011 3048 6
Size: 1005 2037 455
Fragments arranged by size:
2037 1005 455
BsaHI Gr'CG_yC
Cuts at: 928 1176 1878 3374 928
Size: 248 702 1496 1051
Fragments arranged by size:
1496 1051 702 248
BsaWI w'CCGG_w
Cuts at: 686 1207 1885 2159 2306 2717 686
Size: 521 678 274 147 411 1466
Fragments arranged by size:
1466 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 397 397
Size: 3497
BseYI C'CCAG_C
Cuts at: 172 399 922 1680 2257 2753 172
Size: 227 523 758 577 496 916
Fragments arranged by size:
916 758 577 523 496 227
BsiEI CG_ry'CG
Cuts at: 104 1039 1085 2293 104
Size: 935 46 1208 1308
Fragments arranged by size:
1308 1208 935 46
BsiHKAI G_wGCw'C
Cuts at: 203 389 511 1289 1479 2271 203
Size: 186 122 778 190 792 1429
Fragments arranged by size:
1429 792 778 190 186 122
Bsp1286I G_dGCh'C
Cuts at: 203 389 511 1122 1215 1289 1479 1541 2271
Size: 186 122 611 93 74 190 62 730
Cuts at: 2271 203
Size: 1429
Fragments arranged by size:
1429 730 611 190 186 122 93 74
62
BspEI T'CCGG_A
Cuts at: 2717 2717
Size: 3497
BspHI T'CATG_A
Cuts at: 2673 2673
Size: 3497
BspMI ACCTGCnnnn'nnnn_
Cuts at: 547 761 1063 1444 547
Size: 214 302 381 2600
Fragments arranged by size:
2600 381 302 214
BsrBI CCG'CTC
Cuts at: 50 161 234 1789 1843 3327 3492 50
Size: 111 73 1555 54 1484 165 55
Fragments arranged by size:
1555 1484 165 111 73 55 54
BsrDI GCAATG_nn'
Cuts at: 318 1409 318
Size: 1091 2406
BsrFI r'CCGG_y
Cuts at: 244 1495 1676 1885 244
Size: 1251 181 209 1856
Fragments arranged by size:
1856 1251 209 181
BssHII G'CGCG_C
Cuts at: 248 248
Size: 3497
BssSI C'ACGA_G
Cuts at: 1768 2126 1768
Size: 358 3139
BstAPI GCAn_nnn'nTGC
Cuts at: 207 874 207
Size: 667 2830
BstBI TT'CG_AA
Cuts at: 1858 1858
Size: 3497
BstYI r'GATC_y
Cuts at: 1 293 522 989 1012 1347 1593 2605 2691
Size: 292 229 467 23 335 246 1012 86
Cuts at: 2691 2703 3049 1
Size: 12 346 449
Fragments arranged by size:
1012 467 449 346 335 292 246 229
86 23 12
BtgI C'CryG_G
Cuts at: 188 188
Size: 3497
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 399 1447 1628 1791 399
Size: 1048 181 163 2105
Fragments arranged by size:
2105 1048 181 163
BtsI GCAGTG_nn'
Cuts at: 740 740
Size: 3497
DraI TTT'AAA
Cuts at: 496 496
Size: 3497
DrdI GACnn_nn'nnGTC
Cuts at: 1203 2061 1203
Size: 858 2639
EaeI y'GGCC_r
Cuts at: 365 1082 1256 1647 1674 3019 365
Size: 717 174 391 27 1345 843
Fragments arranged by size:
1345 843 717 391 174 27
EagI C'GGCC_G
Cuts at: 1082 1082
Size: 3497
EarI CTCTTCn'nnn_
Cuts at: 168 1520 1730 168
Size: 1352 210 1935
Fragments arranged by size:
1935 1352 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1705 2015 2161 3307 1705
Size: 310 146 1146 1895
Fragments arranged by size:
1895 1146 310 146
EcoICRI GAG'CTC
Cuts at: 387 509 387
Size: 122 3375
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 163 295 319 1322 1754 2501 2769 163
Size: 132 24 1003 432 747 268 891
Fragments arranged by size:
1003 891 747 432 268 132 24
EcoO109I rG'GnC_Cy
Cuts at: 636 636
Size: 3497
EcoRI G'AATT_C
Cuts at: 501 501
Size: 3497
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1516 1516
Size: 3497
FspI TGC'GCA
Cuts at: 1278 1278
Size: 3497
HaeII r_GCGC'y
Cuts at: 931 1179 2201 3180 931
Size: 248 1022 979 1248
Fragments arranged by size:
1248 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1339 1369 1371 1401 1339
Size: 30 2 30 3435
Fragments arranged by size:
3435 30 30 2
HincII GTy'rAC
Cuts at: 536 536
Size: 3497
KasI G'GCGC_C
Cuts at: 927 1175 927
Size: 248 3249
KpnI G_GTAC'C
Cuts at: 517 517
Size: 3497
MluI A'CGCG_T
Cuts at: 2930 2930
Size: 3497
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 219 384 2168 2352 219
Size: 165 1784 184 1364
Fragments arranged by size:
1784 1364 184 165
MscI TGG'CCA
Cuts at: 367 1258 367
Size: 891 2606
MslI CAynn'nnrTG
Cuts at: 1486 1613 1486
Size: 127 3370
MspA1I CmG'CkG
Cuts at: 289 608 922 1282 2295 2540 289
Size: 319 314 360 1013 245 1246
Fragments arranged by size:
1246 1013 360 319 314 245
NaeI GCC'GGC
Cuts at: 246 1678 246
Size: 1432 2065
NarI GG'CG_CC
Cuts at: 928 1176 928
Size: 248 3249
NgoMIV G'CCGG_C
Cuts at: 244 1676 244
Size: 1432 2065
NspI r_CATG'y
Cuts at: 550 1050 1957 550
Size: 500 907 2090
Fragments arranged by size:
2090 907 500
PciI A'CATG_T
Cuts at: 1953 1953
Size: 3497
PfoI T'CCnGG_A
Cuts at: 296 296
Size: 3497
PmeI GTTT'AAAC
Cuts at: 496 496
Size: 3497
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2636 2668 2636
Size: 32 3465
PstI C_TGCA'G
Cuts at: 544 1229 544
Size: 685 2812
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3497
PvuII CAG'CTG
Cuts at: 922 1282 922
Size: 360 3137
RsrII CG'GwC_CG
Cuts at: 1692 1692
Size: 3497
SacI G_AGCT'C
Cuts at: 389 511 389
Size: 122 3375
SalI G'TCGA_C
Cuts at: 534 534
Size: 3497
SapI GCTCTTCn'nnn_
Cuts at: 168 1520 1730 168
Size: 1352 210 1935
Fragments arranged by size:
1935 1352 210
SbfI CC_TGCA'GG
Cuts at: 544 544
Size: 3497
ScaI AGT'ACT
Cuts at: 3014 3014
Size: 3497
SfcI C'TryA_G
Cuts at: 33 540 1225 2218 2409 33
Size: 507 685 993 191 1121
Fragments arranged by size:
1121 993 685 507 191
SfoI GGC'GCC
Cuts at: 929 1177 929
Size: 248 3249
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3497
SmaI CCC'GGG
Cuts at: 519 519
Size: 3497
SmlI C'TyrA_G
Cuts at: 652 1567 2059 2321 652
Size: 915 492 262 1828
Fragments arranged by size:
1828 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3497
SphI G_CATG'C
Cuts at: 550 1050 550
Size: 500 2997
StyI C'CwwG_G
Cuts at: 146 631 985 146
Size: 485 354 2658
Fragments arranged by size:
2658 485 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1080 1213 1672 1881 1080
Size: 133 459 209 2696
Fragments arranged by size:
2696 459 209 133
TatI w'GTAC_w
Cuts at: 3012 3012
Size: 3497
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3014 3046 3014
Size: 32 3465
Tth111I GACn'n_nGTC
Cuts at: 1294 1294
Size: 3497
XbaI T'CTAG_A
Cuts at: 528 528
Size: 3497
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 992 992
Size: 3497
XmaI C'CCGG_G
Cuts at: 517 517
Size: 3497
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AscI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PciI PfoI
PmeI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AvrII BaeI BbvCI BcgI BfrBI BglI BmtI
Bpu10I BsaXI BsgI BsiWI BsmI BsmBI BsrGI BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI