Restriction map for
pF1KE0232
(Circular) (Six-base) MAPSORT of: pF1KE0232.seq Check: 5355 from: 1 to: 4049
pF1KE0232 4049 bp
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 176 176
Size: 4049
AccI GT'mk_AC
Cuts at: 1087 1087
Size: 4049
Acc65I G'GTAC_C
Cuts at: 1065 1065
Size: 4049
AclI AA'CG_TT
Cuts at: 3889 3889
Size: 4049
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 799 1874 2306 3053 3321 799
Size: 1075 432 747 268 1527
Fragments arranged by size:
1527 1075 747 432 268
AflIII A'CryG_T
Cuts at: 206 1401 2505 3482 206
Size: 1195 1104 977 773
Fragments arranged by size:
1195 1104 977 773
AgeI A'CCGG_T
Cuts at: 2437 2437
Size: 4049
AleI CACnn'nnGTG
Cuts at: 250 250
Size: 4049
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 911 943 911
Size: 32 4017
AlwNI CAG_nnn'CTG
Cuts at: 1354 2921 1354
Size: 1567 2482
ApaLI G'TGCA_C
Cuts at: 2819 2819
Size: 4049
ApoI r'AATT_y
Cuts at: 294 433 601 1053 3402 3658 294
Size: 139 168 452 2349 256 685
Fragments arranged by size:
2349 685 452 256 168 139
AseI AT'TA_AT
Cuts at: 20 20
Size: 4049
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4049
AvaI C'yCGr_G
Cuts at: 1069 1069
Size: 4049
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 665 698 665
Size: 33 4016
BamHI G'GATC_C
Cuts at: 1074 1074
Size: 4049
BanI G'GyrC_C
Cuts at: 1065 1479 1727 1762 1065
Size: 414 248 35 3352
Fragments arranged by size:
3352 414 248 35
BanII G_rGCy'C
Cuts at: 1063 2093 1063
Size: 1030 3019
BbeI G_GCGC'C
Cuts at: 1483 1731 1483
Size: 248 3801
BciVI GTATCCnnnnn_n'
Cuts at: 1947 2708 4049 1947
Size: 761 1341 1947
Fragments arranged by size:
1947 1341 761
BclI T'GATC_A
Cuts at: 563 624 1569 563
Size: 61 945 3043
Fragments arranged by size:
3043 945 61
BglII A'GATC_T
Cuts at: 659 1564 3601 659
Size: 905 2037 1107
Fragments arranged by size:
2037 1107 905
BlpI GC'TnA_GC
Cuts at: 1161 1161
Size: 4049
Bme1580I G_kGCm'C
Cuts at: 1674 1767 2823 1674
Size: 93 1056 2900
Fragments arranged by size:
2900 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1261 1342 1677 3821 1261
Size: 81 335 2144 1489
Fragments arranged by size:
2144 1489 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2416 2416
Size: 4049
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 577 577
Size: 4049
Bpu10I CC'TnA_GC
Cuts at: 585 585
Size: 4049
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 996 1225 2104 2596 2894 996
Size: 229 879 492 298 2151
Fragments arranged by size:
2151 879 492 298 229
BsaI GGTCTCn'nnnn_
Cuts at: 3753 3753
Size: 4049
BsaAI yAC'GTr
Cuts at: 1402 1402
Size: 4049
BsaBI GATnn'nnATC
Cuts at: 6 1563 3600 6
Size: 1557 2037 455
Fragments arranged by size:
2037 1557 455
BsaHI Gr'CG_yC
Cuts at: 1480 1728 2430 3926 1480
Size: 248 702 1496 1603
Fragments arranged by size:
1603 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1238 1759 2437 2711 2858 3269 1238
Size: 521 678 274 147 411 2018
Fragments arranged by size:
2018 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 911 941 911
Size: 30 4019
BseYI C'CCAG_C
Cuts at: 144 1474 2232 2809 3305 144
Size: 1330 758 577 496 888
Fragments arranged by size:
1330 888 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1591 1637 2845 104
Size: 1487 46 1208 1308
Fragments arranged by size:
1487 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1063 1841 2031 2823 1063
Size: 778 190 792 2289
Fragments arranged by size:
2289 792 778 190
BsmI GAATG_Cn'
Cuts at: 332 332
Size: 4049
Bsp1286I G_dGCh'C
Cuts at: 1063 1674 1767 1841 2031 2093 2823 1063
Size: 611 93 74 190 62 730 2289
Fragments arranged by size:
2289 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3269 3269
Size: 4049
BspHI T'CATG_A
Cuts at: 506 3225 506
Size: 2719 1330
BspMI ACCTGCnnnn'nnnn_
Cuts at: 176 634 1099 1313 1615 1996 176
Size: 458 465 214 302 381 2229
Fragments arranged by size:
2229 465 458 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2341 2395 3879 4044 50
Size: 2291 54 1484 165 55
Fragments arranged by size:
2291 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 848 1961 848
Size: 1113 2936
BsrFI r'CCGG_y
Cuts at: 2047 2228 2437 2047
Size: 181 209 3659
Fragments arranged by size:
3659 209 181
BsrGI T'GTAC_A
Cuts at: 347 758 347
Size: 411 3638
BssSI C'ACGA_G
Cuts at: 2320 2678 2320
Size: 358 3691
BstAPI GCAn_nnn'nTGC
Cuts at: 177 1426 177
Size: 1249 2800
BstBI TT'CG_AA
Cuts at: 2410 2410
Size: 4049
BstEII G'GTnAC_C
Cuts at: 157 157
Size: 4049
BstYI r'GATC_y
Cuts at: 1 659 1074 1541 1564 1899 2145 3157 3243
Size: 658 415 467 23 335 246 1012 86
Cuts at: 3243 3255 3601 1
Size: 12 346 449
Fragments arranged by size:
1012 658 467 449 415 346 335 246
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1999 2180 2343 1999
Size: 181 163 3705
Fragments arranged by size:
3705 181 163
BtsI GCAGTG_nn'
Cuts at: 1292 1292
Size: 4049
DraI TTT'AAA
Cuts at: 1048 1048
Size: 4049
DrdI GACnn_nn'nnGTC
Cuts at: 1755 2613 1755
Size: 858 3191
EaeI y'GGCC_r
Cuts at: 473 905 1634 1808 2199 2226 3571 473
Size: 432 729 174 391 27 1345 951
Fragments arranged by size:
1345 951 729 432 391 174 27
EagI C'GGCC_G
Cuts at: 1634 1634
Size: 4049
EarI CTCTTCn'nnn_
Cuts at: 2072 2282 2072
Size: 210 3839
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2257 2567 2713 3859 2257
Size: 310 146 1146 2447
Fragments arranged by size:
2447 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1061 1061
Size: 4049
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 577 799 1874 2306 3053 3321 577
Size: 222 1075 432 747 268 1305
Fragments arranged by size:
1305 1075 747 432 268 222
EcoO109I rG'GnC_Cy
Cuts at: 646 1188 646
Size: 542 3507
EcoRI G'AATT_C
Cuts at: 601 1053 601
Size: 452 3597
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 196 2068 196
Size: 1872 2177
FspI TGC'GCA
Cuts at: 1830 1830
Size: 4049
HaeII r_GCGC'y
Cuts at: 1483 1731 2753 3732 1483
Size: 248 1022 979 1800
Fragments arranged by size:
1800 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1891 1921 1923 1953 1891
Size: 30 2 30 3987
Fragments arranged by size:
3987 30 30 2
HincII GTy'rAC
Cuts at: 1088 1088
Size: 4049
HindIII A'AGCT_T
Cuts at: 998 998
Size: 4049
KasI G'GCGC_C
Cuts at: 1479 1727 1479
Size: 248 3801
KpnI G_GTAC'C
Cuts at: 1069 1069
Size: 4049
MluI A'CGCG_T
Cuts at: 3482 3482
Size: 4049
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2720 2904 2720
Size: 184 3865
MscI TGG'CCA
Cuts at: 475 907 1810 475
Size: 432 903 2714
Fragments arranged by size:
2714 903 432
MslI CAynn'nnrTG
Cuts at: 205 250 838 2038 2165 205
Size: 45 588 1200 127 2089
Fragments arranged by size:
2089 1200 588 127 45
MspA1I CmG'CkG
Cuts at: 1160 1474 1834 2847 3092 1160
Size: 314 360 1013 245 2117
Fragments arranged by size:
2117 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2230 2230
Size: 4049
NarI GG'CG_CC
Cuts at: 1480 1728 1480
Size: 248 3801
NgoMIV G'CCGG_C
Cuts at: 2228 2228
Size: 4049
NspI r_CATG'y
Cuts at: 210 1102 1602 2509 210
Size: 892 500 907 1750
Fragments arranged by size:
1750 907 892 500
PciI A'CATG_T
Cuts at: 206 2505 206
Size: 2299 1750
PmeI GTTT'AAAC
Cuts at: 1048 1048
Size: 4049
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 911 943 3188 3220 911
Size: 32 2245 32 1740
Fragments arranged by size:
2245 1740 32 32
PpuMI rG'GwC_Cy
Cuts at: 646 646
Size: 4049
PstI C_TGCA'G
Cuts at: 1096 1781 1096
Size: 685 3364
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4049
PvuII CAG'CTG
Cuts at: 1474 1834 1474
Size: 360 3689
RsrII CG'GwC_CG
Cuts at: 2244 2244
Size: 4049
SacI G_AGCT'C
Cuts at: 1063 1063
Size: 4049
SalI G'TCGA_C
Cuts at: 1086 1086
Size: 4049
SapI GCTCTTCn'nnn_
Cuts at: 2072 2282 2072
Size: 210 3839
SbfI CC_TGCA'GG
Cuts at: 1096 1096
Size: 4049
ScaI AGT'ACT
Cuts at: 3566 3566
Size: 4049
SfcI C'TryA_G
Cuts at: 33 323 1092 1777 2770 2961 33
Size: 290 769 685 993 191 1121
Fragments arranged by size:
1121 993 769 685 290 191
SfoI GGC'GCC
Cuts at: 1481 1729 1481
Size: 248 3801
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4049
SmaI CCC'GGG
Cuts at: 1071 1071
Size: 4049
SmlI C'TyrA_G
Cuts at: 975 1204 2119 2611 2873 975
Size: 229 915 492 262 2151
Fragments arranged by size:
2151 915 492 262 229
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4049
SphI G_CATG'C
Cuts at: 1102 1602 1102
Size: 500 3549
SspI AAT'ATT
Cuts at: 404 404
Size: 4049
StuI AGG'CCT
Cuts at: 590 811 590
Size: 221 3828
StyI C'CwwG_G
Cuts at: 1183 1537 1183
Size: 354 3695
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 537 1632 1765 2224 2433 537
Size: 1095 133 459 209 2153
Fragments arranged by size:
2153 1095 459 209 133
TatI w'GTAC_w
Cuts at: 278 347 758 3564 278
Size: 69 411 2806 763
Fragments arranged by size:
2806 763 411 69
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3566 3598 3566
Size: 32 4017
Tth111I GACn'n_nGTC
Cuts at: 1846 1846
Size: 4049
XbaI T'CTAG_A
Cuts at: 1080 1080
Size: 4049
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1544 1544
Size: 4049
XmaI C'CCGG_G
Cuts at: 1069 1069
Size: 4049
XmnI GAAnn'nnTTC
Cuts at: 297 437 961 297
Size: 140 524 3385
Fragments arranged by size:
3385 524 140
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstEII BstYI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PciI PmeI PpiI PpuMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI ApaI AscI AvrII
BbsI BbvCI BcgI BfrBI BglI BmgBI BmtI BseRI
BsgI BsiWI BsmBI BssHII BstXI BstZ17I Bsu36I BtgI
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HpaI
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmlI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SwaI XhoI ZraI