Restriction map for
pF1KE0234
(Circular) (Six-base) MAPSORT of: pF1KE0234.seq Check: 6204 from: 1 to: 3875
pF1KE0234 3875 bp
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
AccI GT'mk_AC
Cuts at: 913 913
Size: 3875
Acc65I G'GTAC_C
Cuts at: 891 891
Size: 3875
AclI AA'CG_TT
Cuts at: 3715 3715
Size: 3875
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 156 731 1700 2132 2879 3147 156
Size: 575 969 432 747 268 884
Fragments arranged by size:
969 884 747 575 432 268
AflIII A'CryG_T
Cuts at: 645 1227 2331 3308 645
Size: 582 1104 977 1212
Fragments arranged by size:
1212 1104 977 582
AgeI A'CCGG_T
Cuts at: 394 2263 394
Size: 1869 2006
AleI CACnn'nnGTG
Cuts at: 280 280
Size: 3875
AlwNI CAG_nnn'CTG
Cuts at: 179 415 804 1180 2747 179
Size: 236 389 376 1567 1307
Fragments arranged by size:
1567 1307 389 376 236
ApaLI G'TGCA_C
Cuts at: 2645 2645
Size: 3875
ApoI r'AATT_y
Cuts at: 879 3228 3484 879
Size: 2349 256 1270
Fragments arranged by size:
2349 1270 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3875
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3875
AvaI C'yCGr_G
Cuts at: 895 895
Size: 3875
BamHI G'GATC_C
Cuts at: 900 900
Size: 3875
BanI G'GyrC_C
Cuts at: 362 891 1305 1553 1588 362
Size: 529 414 248 35 2649
Fragments arranged by size:
2649 529 414 248 35
BanII G_rGCy'C
Cuts at: 202 491 597 889 1919 202
Size: 289 106 292 1030 2158
Fragments arranged by size:
2158 1030 292 289 106
BbeI G_GCGC'C
Cuts at: 366 1309 1557 366
Size: 943 248 2684
Fragments arranged by size:
2684 943 248
BciVI GTATCCnnnnn_n'
Cuts at: 573 1773 2534 3875 573
Size: 1200 761 1341 573
Fragments arranged by size:
1341 1200 761 573
BclI T'GATC_A
Cuts at: 330 1395 330
Size: 1065 2810
BglI GCCn_nnn'nGGC
Cuts at: 206 680 206
Size: 474 3401
BglII A'GATC_T
Cuts at: 446 1390 3427 446
Size: 944 2037 894
Fragments arranged by size:
2037 944 894
BlpI GC'TnA_GC
Cuts at: 987 987
Size: 3875
Bme1580I G_kGCm'C
Cuts at: 1500 1593 2649 1500
Size: 93 1056 2726
Fragments arranged by size:
2726 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1087 1168 1503 3647 1087
Size: 81 335 2144 1315
Fragments arranged by size:
2144 1315 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 723 2242 723
Size: 1519 2356
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 181 471 661 181
Size: 290 190 3395
Fragments arranged by size:
3395 290 190
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1051 1930 2422 2720 1051
Size: 879 492 298 2206
Fragments arranged by size:
2206 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 403 709 3579 403
Size: 306 2870 699
Fragments arranged by size:
2870 699 306
BsaAI yAC'GTr
Cuts at: 1228 1228
Size: 3875
BsaBI GATnn'nnATC
Cuts at: 6 1389 3426 6
Size: 1383 2037 455
Fragments arranged by size:
2037 1383 455
BsaHI Gr'CG_yC
Cuts at: 188 363 1306 1554 2256 3752 188
Size: 175 943 248 702 1496 311
Fragments arranged by size:
1496 943 702 311 248 175
BsaWI w'CCGG_w
Cuts at: 299 394 1064 1585 2263 2537 2684 3095 299
Size: 95 670 521 678 274 147 411 1079
Fragments arranged by size:
1079 678 670 521 411 274 147 95
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 229 229
Size: 3875
BseYI C'CCAG_C
Cuts at: 1300 2058 2635 3131 1300
Size: 758 577 496 2044
Fragments arranged by size:
2044 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 304 304
Size: 3875
BsiEI CG_ry'CG
Cuts at: 104 1417 1463 2671 104
Size: 1313 46 1208 1308
Fragments arranged by size:
1313 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 151 597 889 1667 1857 2649 151
Size: 446 292 778 190 792 1377
Fragments arranged by size:
1377 792 778 446 292 190
BsmBI CGTCTCn'nnnn_
Cuts at: 811 811
Size: 3875
Bsp1286I G_dGCh'C
Cuts at: 151 202 491 597 889 1500 1593 1667 1857
Size: 51 289 106 292 611 93 74 190
Cuts at: 1857 1919 2649 151
Size: 62 730 1377
Fragments arranged by size:
1377 730 611 292 289 190 106 93
74 62 51
BspEI T'CCGG_A
Cuts at: 299 3095 299
Size: 2796 1079
BspHI T'CATG_A
Cuts at: 3051 3051
Size: 3875
BspMI ACCTGCnnnn'nnnn_
Cuts at: 518 743 925 1139 1441 1822 518
Size: 225 182 214 302 381 2571
Fragments arranged by size:
2571 381 302 225 214 182
BsrBI CCG'CTC
Cuts at: 50 375 2167 2221 3705 3870 50
Size: 325 1792 54 1484 165 55
Fragments arranged by size:
1792 1484 325 165 55 54
BsrDI GCAATG_nn'
Cuts at: 683 1787 683
Size: 1104 2771
BsrFI r'CCGG_y
Cuts at: 394 1873 2054 2263 394
Size: 1479 181 209 2006
Fragments arranged by size:
2006 1479 209 181
BssSI C'ACGA_G
Cuts at: 211 2146 2504 211
Size: 1935 358 1582
Fragments arranged by size:
1935 1582 358
BstAPI GCAn_nnn'nTGC
Cuts at: 415 1252 415
Size: 837 3038
BstBI TT'CG_AA
Cuts at: 2236 2236
Size: 3875
BstXI CCAn_nnnn'nTGG
Cuts at: 696 696
Size: 3875
BstYI r'GATC_y
Cuts at: 1 446 900 1367 1390 1725 1971 2983 3069
Size: 445 454 467 23 335 246 1012 86
Cuts at: 3069 3081 3427 1
Size: 12 346 449
Fragments arranged by size:
1012 467 454 449 445 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 663 107
Size: 556 3319
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1825 2006 2169 1825
Size: 181 163 3531
Fragments arranged by size:
3531 181 163
BtsI GCAGTG_nn'
Cuts at: 1118 1118
Size: 3875
DraI TTT'AAA
Cuts at: 874 874
Size: 3875
DrdI GACnn_nn'nnGTC
Cuts at: 1581 2439 1581
Size: 858 3017
EaeI y'GGCC_r
Cuts at: 110 1460 1634 2025 2052 3397 110
Size: 1350 174 391 27 1345 588
Fragments arranged by size:
1350 1345 588 391 174 27
EagI C'GGCC_G
Cuts at: 1460 1460
Size: 3875
EarI CTCTTCn'nnn_
Cuts at: 283 707 809 1898 2108 283
Size: 424 102 1089 210 2050
Fragments arranged by size:
2050 1089 424 210 102
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2083 2393 2539 3685 2083
Size: 310 146 1146 2273
Fragments arranged by size:
2273 1146 310 146
EcoICRI GAG'CTC
Cuts at: 595 887 595
Size: 292 3583
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 156 181 471 661 731 1700 2132 2879 3147
Size: 25 290 190 70 969 432 747 268
Cuts at: 3147 156
Size: 884
Fragments arranged by size:
969 884 747 432 290 268 190 70
25
EcoO109I rG'GnC_Cy
Cuts at: 1014 1014
Size: 3875
EcoRI G'AATT_C
Cuts at: 879 879
Size: 3875
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1894 1894
Size: 3875
FspI TGC'GCA
Cuts at: 1656 1656
Size: 3875
HaeII r_GCGC'y
Cuts at: 241 366 459 1309 1557 2579 3558 241
Size: 125 93 850 248 1022 979 558
Fragments arranged by size:
1022 979 850 558 248 125 93
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1717 1747 1749 1779 1717
Size: 30 2 30 3813
Fragments arranged by size:
3813 30 30 2
HincII GTy'rAC
Cuts at: 914 914
Size: 3875
KasI G'GCGC_C
Cuts at: 362 1305 1553 362
Size: 943 248 2684
Fragments arranged by size:
2684 943 248
KpnI G_GTAC'C
Cuts at: 895 895
Size: 3875
MluI A'CGCG_T
Cuts at: 3308 3308
Size: 3875
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2546 2730 2546
Size: 184 3691
MscI TGG'CCA
Cuts at: 1636 1636
Size: 3875
MslI CAynn'nnrTG
Cuts at: 280 1864 1991 280
Size: 1584 127 2164
Fragments arranged by size:
2164 1584 127
MspA1I CmG'CkG
Cuts at: 176 986 1300 1660 2673 2918 176
Size: 810 314 360 1013 245 1133
Fragments arranged by size:
1133 1013 810 360 314 245
NaeI GCC'GGC
Cuts at: 2056 2056
Size: 3875
NarI GG'CG_CC
Cuts at: 363 1306 1554 363
Size: 943 248 2684
Fragments arranged by size:
2684 943 248
NcoI C'CATG_G
Cuts at: 107 663 107
Size: 556 3319
NgoMIV G'CCGG_C
Cuts at: 2054 2054
Size: 3875
NspI r_CATG'y
Cuts at: 697 928 1428 2335 697
Size: 231 500 907 2237
Fragments arranged by size:
2237 907 500 231
PasI CC'CwG_GG
Cuts at: 852 852
Size: 3875
PciI A'CATG_T
Cuts at: 2331 2331
Size: 3875
PmeI GTTT'AAAC
Cuts at: 874 874
Size: 3875
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3014 3046 3014
Size: 32 3843
PstI C_TGCA'G
Cuts at: 922 1607 922
Size: 685 3190
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3875
PvuII CAG'CTG
Cuts at: 176 1300 1660 176
Size: 1124 360 2391
Fragments arranged by size:
2391 1124 360
RsrII CG'GwC_CG
Cuts at: 2070 2070
Size: 3875
SacI G_AGCT'C
Cuts at: 597 889 597
Size: 292 3583
SalI G'TCGA_C
Cuts at: 912 912
Size: 3875
SapI GCTCTTCn'nnn_
Cuts at: 1898 2108 1898
Size: 210 3665
SbfI CC_TGCA'GG
Cuts at: 922 922
Size: 3875
ScaI AGT'ACT
Cuts at: 3392 3392
Size: 3875
SfcI C'TryA_G
Cuts at: 33 504 918 1603 2596 2787 33
Size: 471 414 685 993 191 1121
Fragments arranged by size:
1121 993 685 471 414 191
SfoI GGC'GCC
Cuts at: 364 1307 1555 364
Size: 943 248 2684
Fragments arranged by size:
2684 943 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3875
SmaI CCC'GGG
Cuts at: 897 897
Size: 3875
SmlI C'TyrA_G
Cuts at: 1030 1945 2437 2699 1030
Size: 915 492 262 2206
Fragments arranged by size:
2206 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3875
SphI G_CATG'C
Cuts at: 928 1428 928
Size: 500 3375
StyI C'CwwG_G
Cuts at: 107 663 1009 1363 107
Size: 556 346 354 2619
Fragments arranged by size:
2619 556 354 346
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1458 1591 2050 2259 1458
Size: 133 459 209 3074
Fragments arranged by size:
3074 459 209 133
TatI w'GTAC_w
Cuts at: 648 3390 648
Size: 2742 1133
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3392 3424 3392
Size: 32 3843
Tth111I GACn'n_nGTC
Cuts at: 1672 1672
Size: 3875
XbaI T'CTAG_A
Cuts at: 906 906
Size: 3875
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 801 840 1370 801
Size: 39 530 3306
Fragments arranged by size:
3306 530 39
XmaI C'CCGG_G
Cuts at: 895 895
Size: 3875
XmnI GAAnn'nnTTC
Cuts at: 169 620 169
Size: 451 3424
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PmeI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BmgBI
BmtI Bpu10I BsaXI BsiWI BsmI BsrGI BssHII BstEII
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PflMI PfoI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI ZraI